membrane protein – Page 7 – Communicable Diseases Research

Antibody Response to SARS-CoV-2 Membrane Protein in Patients of the Acute and Convalescent Phase of COVID-19

COVID-19의 급성 및 회복기 환자에서 SARS-CoV-2 막 단백질에 대한 항체 반응

Immunology

Published on 2021-08-042022-09-11
Journal: Frontiers in Immunology [Category] MERS, SARS, 진단, 치료법,

MALDI-ToF protein profiling as a potential rapid diagnostic platform for COVID-19

Research article

Published on 2021-08-012022-10-05
Journal: Journal of Mass Spectrometry and Advances in the Clinical Lab [Category] 바이오마커, 변종, 진단,

[키워드] liquid chromatography mass spectrometry ACE2angiotensin-converting enzyme 2 Analysis analyzed angiotensin-converting enzyme 2 area under the curve Asymptomatic AUCarea under the curve Biomarker Cohort Concordance controls Coronavirus disease 2019 COVID-19 COVID-19 pandemic COVID-19 testing COVID-19coronavirus disease 2019 CT cycle threshold demonstrated develop diagnostic Diagnostic method dithiothreitol DTTdithiothreitol E Proteinenvelope protein Emergency use authorization envelope protein EUAemergency use authorization FDAFood and Drug Administration Food and Drug Administration human donor human serum IgAimmunoglobulin A IgGimmunoglobulin G IgMimmunoglobulin M immunoglobulin A Immunoglobulin G immunoglobulin M immunoglobulins inactivated LC-MS limit of detection limitation LoDlimit of detection M Proteinmembrane protein MALDI-ToF MSmatrix-assisted laser desorption/ionization-time of flight mass spectrometry matrix-assisted laser desorption/ionization-time of flight mass spectrometry membrane protein N Proteinnucleocapsid protein nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs Non-invasive NPnasopharyngeal nucleocapsid protein pilot study platform positive individuals Protein proteome RBDReceptor Binding Domain receiver operating characteristic Receptor binding domain Reverse transcriptase quantitative polymerase chain reaction Ribonucleic acid RNARibonucleic acid ROCreceiver operating characteristic RT-qPCR S Proteinspike protein Saliva SARS-CoV-2 SARS-CoV-2 strains SARS-CoV-2 virus SARS-CoV-2severe acute respiratory syndrome coronavirus 2 sensitivity severe acute respiratory syndrome coronavirus 2 specificity specimen Spike protein VEPviral envelope protein viral envelope protein WHOWorld Health Organization with COVID-19 World Health Organization
[DOI] 10.1016/j.jmsacl.2021.09.001 [Article Type] Research article

T cell-mediated response to SARS-CoV-2 in liver transplant recipients with prior COVID-19

COVID-19 이전에 간 이식 환자에서 SARS-CoV-2에 대한 T 세포 매개 반응

Article

Published on 2021-08-012022-09-11
Journal: American journal of transplantation : official jou [Category] MERS, SARS, 진단, 치료기술,

[키워드] 1:1 acute respiratory syndrome age antigens CD4 CD8 Cell-mediated immunity cell-mediated response clinical research/practice Complication complication: infectious control group coronavirus COVID-19 COVID-19 diagnosis cytokine declining Diagnosis ELISA ICS IgG antibodies IgG responses immune immune modulation Immunosuppression immunosuppression/immune modulation impair Infection infection and infectious agents infection and infectious agents - viral infectious infectious agent infectious agents Infectious disease interferon intracellular cytokine staining inverse correlation liver Liver transplantation liver transplantation/hepatology magnitude median membrane membrane protein monitoring: immune. MOST no significant difference no significant differences p value Patient patients proportion recipient recipients respiratory response SARS-CoV-2 SARS-CoV-2 IgG antibody Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus SFU Spearman T cell were measured
[DOI] 10.1111/ajt.16708 PMC 바로가기 [Article Type] Article

Screening Antibodies Raised against the Spike Glycoprotein of SARS-CoV-2 to Support the Development of Rapid Antigen Assays

Article

Published on 2021-07-272022-10-27
Journal: ACS Omega [Category] COVID-19,

[키워드] antibody Antigen antigens Asymptomatic case Biomarker coronavirus Coronavirus-2 country cross-reactivity detection development diagnose Diagnosis diagnostic diagnostic test diagnostic tests diagnostic value disease early stage envelope protein group identify immunoassay immunoassays include infected case Infection lateral flow leading management membrane protein molecular monoclonal antibody nucleocapsid protein promote provided Rapid Rapid diagnostic test Research resources SARS-CoV-2 SARS-CoV-2 spike protein Screening sensitivity serologic serologic tests spike glycoprotein Spike protein Support Surveillance the Spike therapeutic Transmission viral pathogen
[DOI] 10.1021/acsomega.1c01321 PMC 바로가기 [Article Type] Article

Highly feasible immunoprotective multicistronic SARS-CoV-2 vaccine candidate blending novel eukaryotic expression and Salmonella bactofection

새로운 진핵생물 발현과 살모넬라 박토펙션을 혼합한 매우 실현 가능한 면역보호 멀티시스트론 SARS-CoV-2 백신 후보

Article

Published on 2021-07-222022-09-11
Journal: Journal of advanced research [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단,

[키워드] Analysis anti-SARS-CoV-2 immune responses Antibody titers antigen-specific antibody Antiviral CD4 CD8 Cell-mediated immunity Cellular immune response co-expression COVID-19 Cytometry demonstrated determine develop DNA domain effective Efficacy Efficiency elicited epitope Epitopes eukaryotic expressing expression feasible Flow flow cytometry Frame fused gene delivery heptad repeat highest humoral immune responses immunized mice immunofluorescence assay immunogenicity IMPROVE in vivo increase in investigated mechanism membrane protein mice mRNA Multicistronic expression multivalent vaccine neutralizing antibody titers nsp13 offer Open reading frame pandemic peptide polarization Protein protein expression raised RBD recall Receptor-binding domain recorded RNA robust Safe Salmonella SARS-CoV-2 SARS-CoV-2 gene SARS-CoV-2 genes SARS-CoV-2 neutralizing antibody SARS-CoV-2 protein SARS-CoV-2 proteins SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants sera Spike protein splenocyte splenocytes suggested T cell T cells targets Th1 Th1 response Th2 immunity the spike protein the vaccine Vaccine vaccine candidate vaccine construct variant variants were used western blot Western blot analysis
[DOI] 10.1016/j.jare.2021.07.007 PMC 바로가기 [Article Type] Article

Tagged extracellular vesicles with the RBD of the viral spike protein for delivery of antiviral agents against SARS-COV-2 infection

SARS-COV-2 감염에 대한 항바이러스제 전달을 위한 바이러스 스파이크 단백질의 RBD로 태그가 지정된 세포외 소포

Article

Published on 2021-07-102022-09-11
Journal: Journal of controlled release : official journal o [Category] SARS, 치료제,

[키워드] accumulate ACE2 ACE2 receptor antiviral agent Antiviral agents approach binding domain Cell cellular cellular entry develop domain effective encapsulate endocytosed Express Extracellular extracellular vesicle Extracellular vesicles fusion hACE2 highlight in vivo Infection Interaction lung tissue lung tissues membrane membrane protein Membrane proteins modify molecular provide pseudovirus infection RBD Receptor binding domain receptor binding domain (RBD) Receptor-binding domain recognize required Safe SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus siRNA siRNAs specific tissue specific tissues Spike protein spread of COVID-19 suppress Tagged target cells target tissue target tissues targeting the RBD the receptor-binding domain therapeutic potential Therapeutics tissue Transgenic mouse viral spike protein
[DOI] 10.1016/j.jconrel.2021.05.049 PMC 바로가기 [Article Type] Article

Poor humoral and T-cell response to two-dose SARS-CoV-2 messenger RNA vaccine BNT162b2 in cardiothoracic transplant recipients

흉부 이식 수혜자의 2회 용량 SARS-CoV-2 메신저 RNA 백신 BNT162b2에 대한 체액 및 T 세포 반응 불량

Original Paper

Published on 2021-07-092022-09-11
Journal: Clinical Research in Cardiology [Category] MERS, 진단, 치료법,

[키워드] Abbott age analysed anti-SARS-CoV-2 average BioNTech/Pfizer (BNT162b2) vaccine blood sample Blood samples BNT162b2 BNT162b2 vaccine booster dose cellular response control group COVID-19 infection detectable domain domains dose ELISA Euroimmun Evidence evidence of excluded exhibited functional Genscript healthy heart humoral IgG immune response immune responses immunoassays Immunocompromised patient Immunocompromised patients immunogenicity Immunoglobulin infectious agent infectious agents inhibitory interferon interferon-γ lung majority membrane protein Messenger RNA Neutralizing antibodies neutralizing antibody nucleocapsid Organ transplant Patient patients peptides Pfizer-BioNTech Poor prime Qiagen QuantiFERON recipient recipients robust SARS-CoV-2 Seroconversion shown significant increase Specific T-cell response Spike protein stimulated T-cell T-cell Response tested titre Transplant recipients vaccination Vaccine Vaccines Viral viral spike was measured Whole blood
[DOI] 10.1007/s00392-021-01880-5 PMC 바로가기 [Article Type] Original Paper

Inhibition of SARS CoV Envelope Protein by Flavonoids and Classical Viroporin Inhibitors

Microbiology

Published on 2021-07-082022-10-31
Journal: Frontiers in Microbiology [Category] COVID-19,

[키워드] acute respiratory syndrome blocked buffer Cell cell viability assay classical conserved contribute coronavirus E protein Effect effective envelope envelope protein epigallocatechin expressed expression Extracellular family Coronaviridae flavonoid genus Betacoronavirus HEK293 cell inflammasome activation inhibited inhibition inhibitor inhibitors inhibitory activity introduced involved ion channel inhibitors kDa membrane protein novel antiviral drug patch-clamp electrophysiology plasma membrane proliferation Protein quercetin RNA virus SARS CoV SARS CoV-1 SARS-CoV SARS-CoV E SARS-CoV-2 sequence signal peptide single-stranded positive-sense suppress tested transfer transmembrane viability viroporins virus Virus life cycle
[DOI] 10.3389/fmicb.2021.692423 PMC 바로가기 [Article Type] Microbiology

Remdesivir Strongly Binds to RNA-Dependent RNA Polymerase, Membrane Protein, and Main Protease of SARS-CoV-2: Indication From Molecular Modeling and Simulations

Pharmacology

Published on 2021-07-072022-10-31
Journal: Frontiers in Pharmacology [Category] COVID-19,

Recombinant SARS-CoV-2 envelope protein traffics to the trans -Golgi network following amphipol-mediated delivery into human cells

Accelerated Communication

Published on 2021-07-052022-10-30
Journal: The Journal of Biological Chemistry [Category] COVID-19,

[키워드] acute respiratory syndrome AF488, Alexa Flour 488 amphipol antiviral therapies approach Cell cells cellular conserved coronavirus CoV CoV, coronavirus delivery disrupt E protein envelope envelope protein ER, endoplasmic reticulum ERGIC, ER–Golgi complex intermediate compartment expression human cell insertion ion channel LAMP1, lysosomal-associated membrane protein 1 Lysosomes membrane membrane protein Membrane proteins membranes NBD, nitrobenzoxadiazole NIH, National Institutes of Health Particle Pathogenesis plasma membrane Polymer progeny Protein purification recombinant S2-E-NBD, NBD-labeled S2-E S2-E, SARS-CoV-2 envelope protein SARS SARS-CoV-2 envelope SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 TGN, trans-Golgi network trans-Golgi network vesicles WGA, wheat germ agglutinin
[DOI] 10.1016/j.jbc.2021.100940 PMC 바로가기 [Article Type] Accelerated Communication