nsp14 – Page 7 – Communicable Diseases Research

Comparative Genomic Analysis of Rapidly Evolving SARS-CoV-2 Reveals Mosaic Pattern of Phylogeographical Distribution

Research Article

Published on 2020-07-282022-10-29
Journal: mSystems [Category] COVID-19,

SARS-CoV-2 nsp13, nsp14, nsp15 and orf6 function as potent interferon antagonists

Research Article

Published on 2020-06-202022-10-31
Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS,

Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA

530

Published on 2018-01-092022-10-29
Journal: Proceedings of the National Academy of Sciences of [Category] Coronavirus,

[키워드] Accuracy activities acute respiratory syndrome antiviral activity Antiviral compound Antiviral treatment association canonical cofactor complex coronavirus coronavirus RNA CoV CoVs domain drug resistance Efficacy enzyme Evolution explain fold evolution Genetic Genome genomic RNA guanine Health in vitro in vivo inactivation Middle East molecular basis mutagenic Mutation rates Nsp12 nsp14 nucleotide Pathogens pathway polymerase Proofreading provided replaced ribavirin RNA RNA genome RNA polymerase RNA synthesis RNA virus SARS-CoV Significance significantly stability These viruses threat unique viral genome Viral RNA virus replication
[DOI] 10.1073/pnas.1718806115 PMC 바로가기 [Article Type] 530

Proofreading-Deficient Coronaviruses Adapt for Increased Fitness over Long-Term Passage without Reversion of Exoribonuclease-Inactivating Mutations

Research Article

Published on 2017-11-072022-10-29
Journal: mBio [Category] Coronavirus, MERS,

[키워드] accounted adaptive evolution amino acid change catalytic competitive fitness coronavirus CoV cultured cell demonstrated disrupt Exoribonuclease explained fitness function Genotype Hypothesis Increased less MHV murine hepatitis virus mutant mutant virus Mutation mutations nonstructural protein 14 Nsp12 nsp14 nsp14-ExoN nucleoside analogue Nucleoside analogues passage phenotype plus-strand RNA virus Proofreading Protein reduced Replication replication fidelity residue responsible reversion RNA genome RNA synthesis RNA virus RNA viruses RNA-dependent RNA polymerase SARS-CoV selected single-stranded positive-sense stability Support susceptibility susceptible virulence wild-type yielding
[DOI] 10.1128/mBio.01503-17 PMC 바로가기 [Article Type] Research Article

Molecular epidemiological study of feline coronavirus strains in Japan using RT-PCR targeting nsp14 gene

Methodology Article

Published on 2015-03-112022-10-28
Journal: BMC veterinary research [Category] Coronavirus,

[키워드] addition Amino acid Analysis Blood caused clinically clone coding region conserved contribute coronavirus coronavirus strain detect develop Direct Epidemiological study Epidemiology fatal disease Feline coronavirus Feline infectious peritonitis genetic diversity Genome Genotype genotyping heterogeneous Infection Japan NCBI nsp14 PCR PCR method PCR product performed Phylogenetic analysis phylogenetic study phylogenetically predominant prevalent quasispecy Registered reported Result RT-PCR sequence Sequencing serotypes specimen strain Strains supplementary material TGEV type I variant variants virus detection WGS whole genome sequence
[DOI] 10.1186/s12917-015-0372-2 PMC 바로가기 [Article Type] Methodology Article

Biochemical and Structural Insights into the Mechanisms of SARS Coronavirus RNA Ribose 2′-O-Methylation by nsp16/nsp10 Protein Complex

Research Article

Published on 2011-10-132022-10-29
Journal: PLoS Pathogens [Category] Coronavirus,

[키워드] Analysis antiviral drug assays Author biochemical canonical catalytic catalyzed cellular cofactor complex composed coronavirus demonstrated different Donor drug target drugs ENCODE eukaryotic eukaryotic virus found function Immune escape in viral indicated innate immune insight inter-molecular Interaction methylated methyltransferase modify mRNA mRNA stability mRNAs non-structural protein nsp14 promote protein translation required ribose ribosome RNA RNA stability RNAs S-adenosyl-L-methionine SAM SARS Coronavirus SARS-CoV shown stimulatory Structural analysis subunit subunits unique Viral RNA virus
[DOI] 10.1371/journal.ppat.1002294 PMC 바로가기 [Article Type] Research Article

Infidelity of SARS-CoV Nsp14-Exonuclease Mutant Virus Replication Is Revealed by Complete Genome Sequencing

Research Article

Published on 2010-05-062022-10-29
Journal: PLoS Pathogens [Category] Coronavirus,

[키워드] accumulate acute respiratory syndrome Author bioinformatics clone compared competent Complete complex consensus genome coronavirus coronavirus exoribonuclease Coronaviruses Critical Culture decrease deep sequencing demonstrated Effect Evolution examined experiment Frequency full-length genome Genetic Genome genome replication genome sequence growth Impact in vitro inactivation increase in Infidelity lack less limit mechanism MOST murine hepatitis virus mutant mutant virus Mutation nonstructural protein nonstructural protein 14 nsp14 nsp14-ExoN Nsps passage Pathogenesis powerful tool quasispecy recognize Replication replication fidelity required resulting Revealed RNA genome RNA genomes RNA virus SARS-coronavirus SARS-CoV sequence stability the Coronaviridae to define total population unique viral growth viral replication viral RNA genome viral system virus wild-type SARS-CoV
[DOI] 10.1371/journal.ppat.1000896 PMC 바로가기 [Article Type] Research Article

In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation

Research Article

Published on 2010-04-222022-10-29
Journal: PLoS Pathogens [Category] Coronavirus,

[키워드] 2003 Activation activity acute respiratory syndrome addition Author CAP cellular mRNA Complete complex coronavirus Coronaviruses CoV Critical drug design enzyme etiological agent event expression generate Genome host cell in viral in vitro inactive include induce infected cell Influenza virus inhibition assay initiated involved membrane-bound methylated Methylation methyltransferase mRNA mRNAs Mutation non-structural protein nsp14 predicted produced Protein replicate ribosomes RNA RNA genome RNA synthesis RNA transcript S-adenosyl-L-methionine SARS-coronavirus SARS-CoV SARS-CoV infected cells selective sequence target translation variety viral mRNAs viral nonstructural proteins Viral protein virus
[DOI] 10.1371/journal.ppat.1000863 PMC 바로가기 [Article Type] Research Article

Genome-Wide Analysis of Protein-Protein Interactions and Involvement of Viral Proteins in SARS-CoV Replication

Research Article

Published on 2008-10-012022-10-28
Journal: PLoS ONE [Category] Coronavirus,

Analysis of Intraviral Protein-Protein Interactions of the SARS Coronavirus ORFeome

Research Article

Published on 2007-05-232022-10-28
Journal: PLoS ONE [Category] Coronavirus,