A Generic, Scalable, and Rapid Time-Resolved Förster Resonance Energy Transfer-Based Assay for Antigen Detection—SARS-CoV-2 as a Proof of ConceptResearch Article Published on 2021-05-182022-10-29 Journal: mBio [Category] COVID-19, SARS, [키워드] acute respiratory syndrome antibodies Antigen antigen detection antigen test association buffer Cell concept coronavirus coronavirus disease COVID-19 cycle threshold demonstrated diagnostic Donor energy energy transfer Generic increase in Infectious virus Isolation Laboratory Limits of detection mix and read Nasopharyngeal swab nasopharyngeal swabs neutralize pandemic pathogen performed polyclonal positive positive result proof Rapid Rapid diagnostic test Recombinant protein resonance resources reverse transcription-PCR S protein SARS-CoV-2 SARS-CoV-2 nucleoprotein SARS-CoV-2 RT-PCR sensitivity specificity Testing TR-FRET viral infections virus isolation [DOI] 10.1128/mBio.00902-21 PMC 바로가기 [Article Type] Research Article
A bivalent recombinant vaccine targeting the S1 protein induces neutralizing antibodies against both SARS‐CoV‐2 variants and wild‐type of the virusOriginal Article Published on 2021-05-172022-10-30 Journal: MedComm [Category] COVID-19, [키워드] ACE2 binding acute respiratory syndrome antibody production Antigen AS03 B.1.351 variant bivalent vaccine Clinical use composed COVID‐19 D614G E484K effective enzyme exhibited Express hACE2 immunization immunized induce inhibiting K417N mice Mutant strains N501Y neutralization neutralizing antibody other variants pandemic protein subunit vaccine receptor‐binding domain Recombinant protein S1 subunit SARS‐CoV‐2 SARS‐CoV‐2 subunit the S1 protein transgenic mice Vaccine variant virus Virus Disease were measured [DOI] 10.1002/mco2.72 PMC 바로가기 [Article Type] Original Article
A simple, sensitive, and low‐cost FACS assay for detecting antibodies against the native SARS‐CoV‐2 spike protein천연 SARS-CoV-2 스파이크 단백질에 대한 항체를 검출하기 위한 간단하고 민감하며 저렴한 FACS 분석Original Article Published on 2021-05-122022-09-10 Journal: Immunity, Inflammation and Disease [Category] SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antibodies antibody Antibody Response Antibody responses Antibody titer Antibody titers applied Asymptomatic Care CHO cell CHO cells coronavirus COVID‐19 COVID‐19 patient COVID‐19 patients detectable develop Disease progression donors Epidemic expression vector expression vectors FACS flow cytometry Germany GFP Hamburg Health Health care HEK cell help hospital hospitalized patients household contacts human serum identify individual Infectious diseases Infectious virus lab Microscopy MONITOR nuclear Protein Recombinant protein recombinant proteins remained respiratory responses SARS‐CoV‐2 secondary antibody social distancing spike Spike protein Symptom symptomatic contacts the epidemic transfected cell transfected cells trials utility vaccination Virology virus [DOI] 10.1002/iid3.446 PMC 바로가기 [Article Type] Original Article
Review of Covid-19 vaccine clinical trials – A puzzle with missing piecesCovid-19 백신 임상 시험 검토 - 누락된 조각이 있는 퍼즐Review Published on 2021-04-102022-09-10 Journal: International Journal of Biological Sciences [Category] MERS, SARS, 변종, 임상, [키워드] adenoviral vector asymptomatic infection Breastfeeding clinical trial clinical trials COVID-19 pandemic Different subgroups Efficacy enrolled exclusion criteria female geographic location group inactivated Inclusion initial mRNA outbreak pandemic participant pharmaceutical companies phase Pregnancy Protein question Recombinant protein released Research review SARS-CoV-2 subgroup subgroups Trial vaccination Vaccine vaccine candidate vaccine candidates vaccine efficacy Viral viral outbreak viral variant Viral variants [DOI] 10.7150/ijbs.59170 PMC 바로가기 [Article Type] Review
Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal SpeciesPlant Science Published on 2021-04-072022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] Chlamydomoans reinhardtii Chlorella vulgaris Agrobacterium Antigen approach binding domain Chlamydomona Chlorella develop DNA efficient effort Express expression FGF fibroblast geminiviral vector Genetic genetic transformation green algae growth factor Health increase in Infection microalgae microorganism nuclear nuclear transformation nutrient requirements organism overcome platform Protein Proteins provide quantification RBD recombinant Recombinant protein Replication repress Safe SARS-CoV-2 receptor Specy subcloned therapeutic therapeutic proteins Transient vector Viral vector were used western blot [DOI] 10.3389/fpls.2021.650820 PMC 바로가기 [Article Type] Plant Science
Immunotherapy for COVID-19: Evolving treatment of viral infection and associated adverse immunological reactionsCOVID-19에 대한 면역요법: 바이러스 감염 및 관련 면역학적 부작용의 치료 진화Review Published on 2021-04-012022-09-12 Journal: Transfusion and apheresis science : official journ [Category] MERS, SARS, 진단, 치료법, [키워드] administration Adsorption Analysis Anti-viral drugs approved AstraZeneca average biological marker clinical combination vaccine convalescent Coronavirus convalescent plasma Coronavirus COVID-19 Coronavirus inflammation Coronavirus neutralizing antibodies Coronavirus vaccines Course Covid 19 lymphopenia. COVID-19 dose effective Efficacy FDA Guidance immune immunological reaction Immunotherapy include indication infected patient Infection Inflammation lymphopenia Moderna monoclonal antibodies mRNA vaccine Neutralizing neutralizing antibody Pfizer plasma produced Quantitative Recombinant protein recruited Safe simian adenovirus-vectored vaccine single dose stability subjects T-cell Treatment trials Vaccine Vaccines viral infection virus [DOI] 10.1016/j.transci.2021.103093 PMC 바로가기 [Article Type] Review
Application of Viral Vectors for Vaccine Development with a Special Emphasis on COVID-19COVID-19에 특별히 중점을 둔 백신 개발을 위한 바이러스 벡터의 적용Review Published on 2020-11-182022-09-01 Journal: Viruses [Category] SARS, 비임상, 신약개발, 임상, 진단, [키워드] animal model antigens application breath Cancer cancers cellular response clinical trials COVID-19 COVID-19 vaccine COVID-19 vaccines development DNA DNA-based vaccines dose doses elicited evaluated expression flexibility generate Human humoral immunization Infectious disease Infectious diseases lethal doses Neutralizing antibodies neutralizing antibody pathogen Pathogens Protein protein expression Recombinant protein RNA RNA virus RNA-based vaccines Safe self-replicating RNA vectors Trial Tumor tumor cells tumor-associated antigens vaccine candidate Vaccine development vector Viral Viral particles viral surface viral vaccines Viral vector viral vectors viruses [DOI] 10.3390/v12111324 PMC 바로가기 [Article Type] Review
A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2Article Published on 2020-11-182022-10-30 Journal: Molecules [Category] COVID-19, [키워드] addition Alter Ammonium baculovirus bind binding binding affinity Cancer COVID-19 Culture Dipeptidyl peptidase 4 Diseases domain DPP4 ELISA full-length spike protein Human human cell human DPP4 hydrophobic Inflammatory insect cell insect cells insulin resistance Interaction MERS MERS virus novel pharmacological target posttranslational modification procedure produced protease Protein protocol purification question RBD recombinant Recombinant protein SARS-CoV-2 SARS-CoV-2 spike protein Spike protein steatosis surface receptor the SARS-CoV-2 the spike protein therapy type 2 diabete understanding virus were measured [DOI] 10.3390/molecules25225392 PMC 바로가기 [Article Type] Article
Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteinsOriginal Article Published on 2020-11-122023-07-02 Journal: European Journal of Clinical Microbiology & Infect [Category] SARS, [키워드] Colloidal gold immunochromatography assay (GICA) Recombinant protein SARS-CoV-2 Serological diagnosis [DOI] 10.1007/s10096-020-04102-4 PMC 바로가기 [Article Type] Original Article
Yeast-expressed SARS-CoV recombinant receptor-binding domain (RBD219-N1) formulated with aluminum hydroxide induces protective immunity and reduces immune enhancementArticle Published on 2020-11-032023-07-12 Journal: Vaccine [Category] SARS, [키워드] coronavirus Eosinophil infiltration Recombinant protein Severe acute respiratory syndrome Vaccine [DOI] 10.1016/j.vaccine.2020.09.061 PMC 바로가기