Sequencing Using a Two-Step Strategy Reveals High Genetic Diversity in the S Gene of SARS-CoV-2 after a High-Transmission Period in Tunis, Tunisia2단계 전략을 사용한 시퀀싱은 튀니지, 튀니스에서 높은 전염 기간 후 SARS-CoV-2의 S 유전자에서 높은 유전적 다양성을 보여줍니다Research Article Published on 2021-11-102022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 analyzed Characteristics choice collected coronavirus country COVID-19 decrease detectability Diversity effort genetic variability genomic genomic mutations help identify include initial Molecular tests Mutation nasopharyngeal specimen Next-generation sequencing next-generation sequencing technology NGS other variant other variants partial sequence partial sequences pathogenicity performed Period primer recent reported resource respiratory S gene SARS-CoV-2 SARS-CoV-2 variant selected sequenced Sequencing severe acute respiratory syndrome Coronavirus specimen Spike protein strain Strains Strategy Surveillance transmission rate Tunis Tunisia variant variants of concern variants of interest Viral viral S viral strain viral strains virus viruses VoC VOCs VOI VOIs whole-genome sequencing [DOI] 10.1128/Spectrum.00639-21 PMC 바로가기 [Article Type] Research Article
Evaluation of the clinical and analytical performance of the Seegene allplex™ SARS-CoV-2 variants I assay for the detection of variants of concern (VOC) and variants of interests (VOI)관심 변이체(VOC) 및 관심 변이체(VOI) 검출을 위한 Seegene allplex™ SARS-CoV-2 변이체 I 분석의 임상 및 분석 성능 평가Article Published on 2021-11-012022-09-11 Journal: Journal of Clinical Virology [Category] SARS, 변종, 치료기술, [키워드] 95% CI calculated clinical study Clinical use COVID-19 cross-reactivity demonstrated detect diagnostic E484K mutation evaluated gargle genotyping Interference limit of detection microorganism microorganisms Mutation N501Y nasopharyngeal NP swab positive reference standard reproducibility RT-PCR S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 variant SARS-CoV-2 variants sensitivity Sequencing specificity specimen specimens stability Swab variant variant of concern (VOC) Variant of interest (VOI) variants of concern variants of interest VoC WGS whole genome Whole genome sequencing [DOI] 10.1016/j.jcv.2021.104996 PMC 바로가기 [Article Type] Article
Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S geneResearch Article Published on 2021-10-292022-10-28 Journal: PLoS ONE [Category] Coronavirus, MERS, [키워드] Analysis Antigen circulating classical COE Complete concerning conserved contagious coronavirus diarrhoea disease Epidemic epitope etiological agent European Genome identify knowledge molecular morbidity and mortality neutralizing epitope parents PEDV Phylogenetic analysis Poland positive positive result quantitative real-time PCR reveal reverse transcription RT-qPCR S gene sequence single-stranded RNA virus strain Strains substitutions tested unique viral disease virus virus strains [DOI] 10.1371/journal.pone.0258318 PMC 바로가기 [Article Type] Research Article
Development of a Multiplex Tandem PCR (MT-PCR) Assay for the Detection of Emerging SARS-CoV-2 Variants신흥 SARS-COV-2 변이체의 검출을위한 멀티 플렉스 탠덤 PCR (MT-PCR) 분석의 개발Communication Published on 2021-10-082022-09-01 Journal: Viruses [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] absence Alpha Applying approach B.1.1.7 B.1.1.7 lineage Chain Reaction clinical isolates clinical setting Concordance defined detection development evaluated example facilitated FIVE Genome sequencing genomic hospital identify in vitro diagnostic test information international public health isolate K417 Lineage Mutation National nucleic acid nucleotide P681 PCR polymerase chain polymerase chain reaction polymorphism Polymorphisms presence or absence Public health responses reference strain S gene SARS-CoV-2 SARS-CoV-2 lineage Sequence analysis Sequencing single nucleotide Single nucleotide polymorphism SNP tandem turnaround time UK hospital utilised variant variants variants of SARS-CoV-2 [DOI] 10.3390/v13102028 PMC 바로가기 [Article Type] Communication
New infections by SARS-CoV-2 variants of concern after natural infections and post-vaccination in Rio de Janeiro, Brazil브라질 리우데자네이루에서 자연 감염 및 예방 접종 후 우려되는 SARS-CoV-2 변종에 의한 새로운 감염Article Published on 2021-10-012022-09-11 Journal: Infection, genetics and evolution : journal of mol [Category] SARS, 변종, [키워드] analyzed Brazil carried caused ChAdOx1 Clinicians concern CoronaVac dissemination dose doses example First wave FIVE Human identify IgG titer IgG titers Immune escape Immunity Infection infections mild symptoms National Cancer Institute natural infection new infection pandemic Prevalence re-infected Reinfection Rio de Janeiro S gene SARS-CoV-2 SARS-CoV-2 variant sequencing approach sterilizing immunity subject susceptibility the vaccine Transmissibility vaccination Vaccine vaccinee variant variants variants of concern virus VoC [DOI] 10.1016/j.meegid.2021.104998 PMC 바로가기 [Article Type] Article
Evaluation of two RT-PCR screening assays for identifying SARS-CoV-2 variantsSARS-CoV-2 변이체 식별을 위한 두 가지 RT-PCR 스크리닝 분석 평가Article Published on 2021-10-012022-09-11 Journal: Journal of Clinical Virology [Category] SARS, 변종, 치료기술, 치료제, [키워드] analyzed B.1.1.7 B.1.1.7 lineage B.1.1.7 variant B.1.1.7 variants collected COVID-19 determine discrepancies Genome sequencing ID triplex Immune escape indicated Isolation MONITOR positive profile RT-PCR RT-PCR kit S gene SARS CoV-2 SARS-CoV-2 SARS-CoV-2 variant Screen Sequencing significantly higher specimen Spread strain Strains suggested TaqPath Thermofisher triplex variant variants variants of concern variants of concern (VOC) virus load virus spread VoC VOCs [DOI] 10.1016/j.jcv.2021.104969 PMC 바로가기 [Article Type] Article
OmniSARS2: A Highly Sensitive and Specific RT-qPCR-Based COVID-19 Diagnostic Method Designed to Withstand SARS-CoV-2 Lineage EvolutionArticle Published on 2021-09-262022-10-28 Journal: Biomedicines [Category] COVID-19, [키워드] Accuracy affected analytical sensitivity B.1 B.1.1 B.1.1.7 B.1.177 clinical sample Comparative genomics conserved Coronaviridae coronavirus COVID-19 COVID-19 pandemic cross-reactivity detect diagnostic methods diagnostics E gene Evolution Extensive Genome genomic highest human betacoronavirus increase in knowledge less Lineage lineages molecular multiplex RT-qPCR Mutation ORF1ab Pathogens performed Prevalence Probability Region RT-qPCR S gene SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 lineage sensitive sensitivity Specific specificity Strains Taking the SARS-CoV-2 genome transmission of SARS-CoV-2 Viral detection virus viruses Volume while [DOI] 10.3390/biomedicines9101314 PMC 바로가기 [Article Type] Article
A new qualitative RT-PCR assay detecting SARS-CoV-2SARS-COV-2를 검출하는 새로운 정 성적 RT-PCR 분석Article Published on 2021-09-232022-09-01 Journal: Scientific Reports [Category] SARS, 진단, 치료기술, 치료법, [키워드] (RdRp) Biotechnology carried caused choice Complete COVID-19 infections Critical detect Diagnosis diagnosis of COVID-19 diagnostic distribution envelope except for exceptional infections Internal control Laboratory Microbiology nucleic acid nucleic acid extraction nucleocapsid open overcome pandemic probe RdRP Real-time PCR RNA polymerase RNA-dependent RNA polymerase RT-PCR RT-PCR assay S gene SARS-CoV-2 Virology β-actin [DOI] 10.1038/s41598-021-98114-5 PMC 바로가기 [Article Type] Article
Next-Generation Sequencing Reveals Four Novel Viruses Associated with Calf DiarrheaArticle Published on 2021-09-232022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Amino acid Analysis bovine bovine astrovirus Bovine coronavirus bovine kobuvirus bovine norovirus calf calf diarrhea common disease Complete conserved develop diarrhea disease effective Genome genome analysis help involved knowledge lowest Mutation Next-generation sequencing novel positive S gene Sequencing similarity strain tested the S1 subunit virus [DOI] 10.3390/v13101907 PMC 바로가기 [Article Type] Article
Molecular evolutionary characteristics of SARS‐CoV‐2 emerging in the United StatesResearch Article Published on 2021-09-202022-10-04 Journal: Journal of medical virology [Category] SARS, 변종, 진단, [키워드] addition Amino acid beta coronavirus bioinformatics Bioinformatics analysis calculate Characteristics coding decrease Evolution Genome Health highly pathogenic Hypothesis medium molecular molecular evolution Mutation nonsynonymous mutation nucleotide Positive selection RNA viruses S gene S protein SARS‐CoV‐2 SARS‐CoV‐2 sequence Seven Spike protein statistically significant Surveillance the United State The United States virus virus variants was used were used whole genome whole genome sequence [DOI] 10.1002/jmv.27331 PMC 바로가기 [Article Type] Research Article