Bi-ECDAQ: An electrochemical dual-immuno-biosensor accompanied by a customized bi-potentiostat for clinical detection of SARS-CoV-2 Nucleocapsid proteinsBi-ECDAQ: SARS-CoV-2 뉴클레오캡시드 단백질의 임상 검출을 위한 맞춤형 이중-전위기(bi-potentiostat)와 함께 제공되는 전기화학적 이중 면역 바이오센서Article Published on 2022-05-012022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, 치료법, [키워드] accompanied acquisition active site active sites All-in-one data acquisition platform approach Bi-potentiostat readout system carboxyl comparable complex COVID-19 COVID-19 patient Data acquisition diagnostics Diseases Dual working screen-printed electrode Electrochemical immunosensing eliminating flexible functional incompatible lack limit of detection matrix effect N-protein Nasopharyngeal swab nasopharyngeal swab sample nucleocapsid protein offered performed point-of-care Protein Recognition resulting SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 nucleocapsid protein Screen sensitive assay sensitivity significantly lower unmet need [DOI] 10.1016/j.bios.2022.114018 PMC 바로가기 [Article Type] Article
Longitudinal Randomized Cohort Study of SARS-CoV-2 Antibody Seroprevalence in the St. Petersburg Population상트페테르부르크 인구에서 SARS-CoV-2 항체 혈청유병성에 대한 종단 무작위 코호트 연구Randomized Controlled Trial Published on 2022-04-272022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 임상, 진단, 치료기술, 치료법, [키워드] 95% CI Adults age anti-RBD antibodies antibody Antigen antigen receptor antigen receptor binding domain approved calculated calculator carried characteristic children Cohort conducted confidence Confidence intervals coronavirus coronavirus SARS-CoV-2 COVID-19 COVID-19 pandemic COVID-19 patient decrease died effort enzyme enzyme immunoassays FIVE form herd immunity Immunity immunoassays implementation increase in indicated interval less longitudinal material methodology nucleocapsid nucleocapsid antigen Older organized pandemic participated Participation Pasteur Institute percentage percentages Population proportion Quantitative Randomized RBD Receptor binding Receptor binding domain required Russia SARS-CoV-2 SARS-CoV-2 nucleocapsid seropositive Seropositivity Seroprevalence Seven significantly St. Petersburg Stage stages statistical significance statistically statistically significant vaccination Vaccine Vaccines Volunteer volunteers were assessed were recorded z-test [DOI] 10.3390/v14050913 PMC 바로가기 [Article Type] Randomized Controlled Trial
Insights into the specificity for the interaction of the promiscuous SARS-CoV-2 nucleocapsid protein N-terminal domain with deoxyribonucleic acids난잡한 SARS-CoV-2 뉴클레오캡시드 단백질 N-말단 도메인과 데옥시리보핵산의 상호작용에 대한 특이성에 대한 통찰력Article Published on 2022-04-012022-09-11 Journal: International journal of biological macromolecules [Category] COVID19(2023년), SARS, 치료법, [키워드] activity approximation binding Binding specificity cleft dissect DNA DNA binding DNA/RNA binding protein double-stranded feature inhibitors insight Interaction Local motif multifunctional N-terminal domain non-specific nucleic acid nucleocapsid parameter preference Protein RNA RNA binding protein SARS-CoV-2 nucleocapsid SARS-CoV-2 nucleocapsid protein sequence specificity starting point suggested the N protein therapeutic target thermodynamic thymidine Transcription transcriptional regulatory sequence transcriptional regulatory sequences TRS virus infectivity β-sheet [DOI] 10.1016/j.ijbiomac.2022.01.121 PMC 바로가기 [Article Type] Article
Unraveling the antiviral activity of plitidepsin against SARS-CoV-2 by subcellular and morphological analysis세포내 및 형태학적 분석을 통한 SARS-CoV-2에 대한 플리티뎁신의 항바이러스 활성 규명Article Published on 2022-04-012022-09-11 Journal: Antiviral Research [Category] Coronavirus, COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] absence Affect Alpha Analysis antitumor agent Antiviral antiviral activity antiviral drugs Antiviral treatment approach biogenesis block caused Cell Complete coronavirus COVID-19 double-stranded RNA dsRNA Efficacy electron electron microscopy elongation factor eukaryotic translation evaluate the effect Extracellular genome replication group host protein infected Vero E6 cells Interaction labeling mechanism mechanism of action Microscopy morphogenesis Morphological morphological analysis New coronavirus new coronavirus SARS-CoV-2 offer organelle pandemic plasma membrane Plitidepsin Post-infection progeny Protein provided SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid shown Structure subcellular Support target TEM transmission electron microscopy treated unique Vero E6 Vero E6 cell VERO E6 cells Vesicle viral infection Viral particles viral replication virion virus viruses [DOI] 10.1016/j.antiviral.2022.105270 PMC 바로가기 [Article Type] Article
Identification of Natural SARS-CoV-2 Infection in Seroprevalence Studies Among Vaccinated Populations백신 접종 집단 사이의 혈청유병성 연구에서 자연적인 SARS-CoV-2 감염의 확인Article Published on 2022-04-012022-09-11 Journal: Mayo Clinic proceedings [Category] COVID19(2023년), MERS, SARS, 진단, 치료법, [키워드] 95% CI Accuracy age All participants an area antibody Antibody assays Antibody testing Area Blood can not capillary capillary blood Cohort composed conducted determine determined by elevated enrolled enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay female Health care Health care worker health care workers identification IgG IgG antibodies IgG antibody IgGs individual individuals Infection MOST natural natural infection nucleocapsid nucleocapsid protein participant Participants performed Population predicted Protein reactive reactivity reported SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 nucleocapsid separated Seroprevalence significantly specificity Spike protein Spike proteins statistically vaccinated individuals vaccination Vaccination Status was determined was tested were assessed [DOI] 10.1016/j.mayocp.2022.02.002 PMC 바로가기 [Article Type] Article
SARS COV-2 anti-nucleocapsid and anti-spike antibodies in an emergency department healthcare worker cohort: September 2020 – April 2021응급실 의료 종사자 집단의 SARS COV-2 항뉴클레오캡시드 및 항스파이크 항체: 2020년 9월 - 2021년 4월Article Published on 2022-04-012022-09-11 Journal: The American journal of emergency medicine [Category] COVID19(2023년), MERS, SARS, 진단, 치료법, [키워드] 95% CI age anti-spike antibodies anti-spike antibody antibody antibody level antibody levels Antibody Response Antigen baseline BNT162b2 Breakthrough infection breakthrough infections conducted Course COVID-19 infection decrease described emergency department examined Factor Healthcare system healthcare worker IgG antibodies IgG antibody immunogenicity incidence Infection mRNA mRNA-1273 nucleocapsid nucleocapsid antigen participant positive predictor prospective cohort study reduced SARS-CoV-2 SARS-CoV-2 antibodies SARS-COV-2 infection SARS-CoV-2 nucleocapsid SARS-CoV-2 spike antigen seronegative participant strained tested the antibody response the SARS-CoV-2 threshold vaccination Vaccine Vaccine immunogenicitiy. were measured [DOI] 10.1016/j.ajem.2022.01.055 PMC 바로가기 [Article Type] Article
Development of magnetic particle-based chemiluminescence immunoassay for measurement of SARS-CoV-2 nucleocapsid proteinSARS-CoV-2 뉴클레오캡시드 단백질 측정을 위한 자성입자 기반 화학발광 면역분석법 개발Article Published on 2022-04-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료법, 치료제, [키워드] addition antibody antigen detection BALB/c mice capture caused Cell cell line cells chemiluminescence Chemiluminescence enzyme immunoassay chemiluminescence immunoassay Clinical diagnosis coding sequence Coefficient of variance coronavirus disease Coronavirus disease 2019 COVID-19 detection antibody develop development diagnostic disease E. coli cell E. coli cells ELISA enzyme enzyme immunoassay enzyme-linked immunosorbent enzyme-linked immunosorbent assay evaluated expressed expression fused global pandemic highest immunized immunoassay IPTG kDa mAb mAbs molecular weight monoclonal antibodies monoclonal antibody nucleic acid Nucleic acid detection nucleocapsid protein pandemic disease Precision Protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid secreted selected sensitivity spleen Spread structural protein variance was obtained [DOI] 10.1016/j.jviromet.2022.114486 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 nucleocapsid antigen from serum can aid in timing of COVID-19 infection혈청에서 SARS-CoV-2 뉴클레오캡시드 항원 검출은 COVID-19 감염 시기에 도움이 될 수 있습니다Article Published on 2022-04-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료법, [키워드] acute COVID-19 Analytical specificity Antibody testing Antigen antigenemia Combination convalescent phase COVID-19 COVID-19 disease COVID-19 infection COVID-19 patient demonstrated detection diagnostic disease enzyme enzyme immunoassay enzyme-linked immunosorbent enzyme-linked immunosorbent assay information Kinetics marker median N antigen nucleocapsid nucleocapsid antigen onset of disease onset of symptoms Patient PCR PCR positive PCR-confirmed PCR-confirmed COVID-19 plasma samples positive positive PCR resolved respiratory respiratory sample Respiratory samples RNA SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 nucleocapsid SARS-CoV-2 RNA sensitivity serum serum and plasma serum sample serum samples specificity specimen specimens upper respiratory tract URT was collected [DOI] 10.1016/j.jviromet.2022.114469 PMC 바로가기 [Article Type] Article
Targeting SARS-CoV-2 nucleocapsid oligomerization: Insights from molecular docking and molecular dynamics simulationsSARS-CoV-2 뉴클레오캡시드 올리고머화 표적화: 분자 도킹 및 분자 역학 시뮬레이션의 통찰력Article Published on 2022-04-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, [키워드] Analysis anti-N drugs antiviral drug antiviral drugs binding binding affinity C-terminal C-terminal domain caused complex complexes Compound computational approach COVID-19 CTD distribution drug drugs effective examined filament FIVE followed by free energy free energy landscape highest binding affinity hydrogen Hydrogen bond increase indicated infect inhibitor insight MD simulation molecular molecular docking molecular dynamics Molecular dynamics simulation N protein nucleocapsid oligomerization outbreak packaged parameter PCA PDB population distribution potential mechanism potential mechanisms Protein Rapamycin Replication RMSD RMSF Sapanisertib SARS-CoV-2 nucleocapsid SARS-CoV-2 virus self-assembly Silmitasertib stability targeting the SARS-CoV-2 virus Transcription Viral protein Viral RNA virions [DOI] 10.1080/07391102.2020.1839563 PMC 바로가기 [Article Type] Article
Evaluation of saliva self-collection devices for SARS-CoV-2 diagnosticsSARS-CoV-2 진단을 위한 타액 자가 수집 장치 평가Article Published on 2022-03-252022-09-11 Journal: BMC Infectious Diseases [Category] SARS, 치료기술, 치료법, [키워드] affected analyzed assistance bottleneck buffer Community condition COVID-19 pandemic current determined by diagnostic Diagnostic method diagnostic testing diagnostics evaluate evaluated expand funnel implementation incubated individual individuals Large-scale testing no significant difference Pathogens problems professionals profiles quantity respiratory pathogen Respiratory pathogens RNase P Safe Saliva SARS-CoV-2 SARS-CoV-2 nucleocapsid sensitivity significant difference Supply chain temperature were used [DOI] 10.1186/s12879-022-07285-7 PMC 바로가기 [Article Type] Article