Full-Length Computational Model of the SARS-CoV-2 Spike Protein and Its Implications for a Viral Membrane Fusion MechanismArticle Published on 2021-06-112022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] binding cause comprehensive view Computational approaches coronavirus COVID-19 death Effect event facilitate flu full-length fusion fusion mechanism highlight Host initiate mechanism membrane membrane fusion Model morbidity neutralizing antibody notable pandemic Proteolytic processing regulate S protein SARS-CoV-2 SARS-CoV-2 virus spike Spike protein the S1 subunit the SARS-CoV-2 the spike protein variant viral entry viral genome viral infection [DOI] 10.3390/v13061126 PMC 바로가기 [Article Type] Article
Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfateResearch Article Published on 2021-06-062022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, SARS, [키워드] ACE2 addition binding block Cell cellular clinical parameter COVID-19 decrease epithelial cell epithelial cells explain expressed expression glycoprotein heparan sulfate host cells human lung hypoxia indicate infection rate inhibit inhibited Interaction lactoferrin lung modulating NRP1 offer pathophysiology pathway Prevent protein level RBD receptor reduce reduced reduction SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein Syndecan-1 the RBD the receptor-binding domain the S1 subunit the spike protein therapeutic Treatment VERO E6 cells [DOI] 10.1080/22221751.2021.1932607 PMC 바로가기 [Article Type] Research Article
Rapid development of neutralizing and diagnostic SARS-COV-2 mouse monoclonal antibodies중화 및 진단용 SARS-COV-2 마우스 단일클론항체의 신속한 개발Article Published on 2021-05-062022-09-01 Journal: Scientific Reports [Category] SARS, 진단, [키워드] accelerated Amino acid angiotensin angiotensin converting enzyme Applied immunology binding cell types correlated COVID-19 pandemic Critical diagnostic enzyme epithelial cells Epitopes functional functions hACE2 immunization immunohistochemical staining immunology Infectious diseases Interaction Lung epithelial cells lung tissue mAb mAbs monoclonal antibody mouse monoclonal antibody Neutralizing Protein Rapid RBD reagent receptor Receptor binding domain Research S1 subunit SARS-CoV-2 screening process sequence Spike protein staining the S1 subunit the spike protein therapeutic Viral viral neutralization [DOI] 10.1038/s41598-021-88809-0 PMC 바로가기 [Article Type] Article
SARS-CoV-2-specific humoral and cellular immunity in two renal transplants and two hemodialysis patients treated with convalescent plasma회복기 혈장으로 치료받은 2명의 신장 이식 및 2명의 혈액투석 환자에서 SARS-CoV-2 특이 체액성 및 세포성 면역Case Reports Published on 2021-05-012022-09-11 Journal: Journal of medical virology [Category] MERS, SARS, 진단, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibody Antibody titers antiviral drug antiviral immunity Antiviral treatment Case report cellular cellular immunity cellular response Cellular responses Chronic kidney disease Clinical improvement clinically close monitoring convalescent convalescent plasma coronavirus COVID-19 Day Deceased described discharged ELISA ELISPOT ELISpot responses enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay Hemodialysis hospital humoral IgG immune response immune responses Immunoglobulin Immunoglobulin G interferon kidney Kidney disease kidney transplant kidney transplant recipient Kidney transplantation Neutralization assay neutralization titer Neutralizing antibody titer Patient patients treated Receptor-binding domain Remdesivir renal respiratory response S1 subunit SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2-specific antibodies SARS-CoV-2-specific antibody severe acute respiratory syndrome Coronavirus specific antibodies Spike protein the receptor-binding domain the S1 subunit the SARS-CoV-2 therapy Transplant Treatment treatment regimen typical symptom typical symptoms undetectable Viral Viral load were infected [DOI] 10.1002/jmv.26840 PMC 바로가기 [Article Type] Case Reports
Structural impact on SARS-CoV-2 spike protein by D614G substitutionReport Published on 2021-04-302022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome added angiotensin-converting enzyme 2 appear bind binding domain biochemical circulating coronavirus COVID-19 pandemic D614G D614G substitution domain dominant epidemiologist Evolution facilitate forms full-length functional glycine Host host receptor increase Infection Interaction investigated less membrane fusion Microscopy modulate Mutation prefusion conformation Premature Prevent Protein S trimer SARS-CoV-2 SARS-CoV-2 spike protein Science spike spike trimer Spread structural rearrangement Structure Substitution the S1 subunit the SARS-CoV-2 the spike protein Vaccine development variant variants of concern viral entry viral spike viral spread [DOI] 10.1126/science.abf2303 PMC 바로가기 [Article Type] Report
Bioinformatic prediction of immunodominant regions in spike protein for early diagnosis of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Bioinformatics Published on 2021-04-082022-10-28 Journal: PeerJ [Category] COVID-19, [키워드] acute respiratory syndrome Amino acid antibody Antibody detection Antigen antigen detection Antigen-capture antigenicity approach Bioinformatic bioinformatics Bioinformatics analysis caused coronavirus COVID-19 Critical detection system early diagnosis epitope expected generate glycosylation site high accuracy hydrophilicity immunodominant immunodominant epitopes Immunodominant fragments mice Mutation nucleic acid Pandemics peptide Region Result S1 subunit SARS-CoV-2 SARS-CoV-2 detection selected sequence specificity spike Spike protein stimulate strong immune response the S1 subunit virus particle [DOI] 10.7717/peerj.11232 PMC 바로가기 [Article Type] Bioinformatics
Endothelial cell damage is the central part of COVID-19 and a mouse model induced by injection of the S1 subunit of the spike protein내피 세포 손상은 COVID-19의 중심 부분이며 스파이크 단백질의 S1 서브유닛을 주입하여 유도된 마우스 모델Article Published on 2021-04-012022-09-11 Journal: Annals of diagnostic pathology [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] ACE2 Analysis Autopsy Behavior Brain Brain tissues C5b-9 caspase caspase 3 caspase-3 cell damage Complication COVID-19 demonstrated detect diagnostic died endothelial Endothelial cell endothelial cell damage endothelial damage envelope eosin examined fatal COVID-19 full length hematoxylin Hematoxylin and eosin host response IL6 immunohistochemistry increased thirst incubated injected injection Localization membrane protein Membrane proteins MFSD2a mice neurologic neurologic signs Neuron neuronal neuronal cell neuronal cells NOS pathologist pathology positive Protein Proteins pseudovirion pseudovirions S1 subunit S2 subunit SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins SARS-CoV2 SHIP1 spike Spike protein subunit subunits symptomatic symptomatic COVID-19 tail the S1 subunit the spike protein tissue TNFα Viral RNA were used wild type [DOI] 10.1016/j.anndiagpath.2020.151682 PMC 바로가기 [Article Type] Article
Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged MiceArticle Published on 2021-03-292022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] activity acute respiratory syndrome adjuvant aged mice antibodies approved B cell cellular immunity coronavirus domain ectodomain Effect effective Efficacy Emergency use full-length globe High dose humans IgG antibody IgG1 IgG2a immune responses immunogenic immunogenicity information inhibiting interferon-γ isotypes low dose mice Neutralizing Neutralizing activity pandemic disease RBD-hACE2 inhibition Receptor binding domain Result S1 and S2 SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike-specific SARS-CoV-2 vaccine secreting spike subunit T cell the S1 subunit vaccination Vaccine vaccine antigen vaccine candidate vaccine dose [DOI] 10.3390/vaccines9040316 PMC 바로가기 [Article Type] Article
Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies인간의 강력한 중화 항체에 의한 SARS-CoV-2 감염의 2가 결합 및 억제를 위한 구조적 기초Article Published on 2021-03-172022-09-12 Journal: Cell Research [Category] Fulltext, SARS, 진단, [키워드] ACE2 acute respiratory syndrome binding candidate contributing to coronavirus coronavirus disease correlation COVID-19 cryo-EM structure Cryoelectron microscopy disrupting enzyme Fab feature full-length IgG Interaction Intervention mechanism Mechanisms of disease monoclonal antibody NAb neutralization neutralizing activities Neutralizing activity neutralizing antibody Protein provide RBD receptor Receptor binding domain S1 subunit SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected individual the S protein the S1 subunit triggering trimeric S protein unique viral spike [DOI] 10.1038/s41422-021-00487-9 PMC 바로가기 [Article Type] Article
SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interfaceSARS-CoV-2 중화 인간 재조합 항체는 RBD-ACE2 인터페이스에서 결합하는 팬데믹 전 건강한 기증자로부터 선택됩니다Article Published on 2021-03-112022-09-10 Journal: Nature Communications [Category] SARS, 변종, 진단, 치료제, [키워드] ACE2 ACE2 receptor ACE2-RBD acute respiratory disease anti-SARS-CoV-2 antibody Antibody therapy binding Biotechnology candidate caused Cell cell entry coronavirus COVID-19 enzyme expressing Expression systems healthy healthy donor human ACE2 human donor IgG independent Infection Infectious diseases inhibit Interaction material naïve neutralization neutralize Neutralizing offer pandemic phage RBD RBD-ACE2 receptor Receptor binding domain recognizing recovering patient Respiratory disease S1 subunit sarbecovirus SARS-CoV-2 SARS-CoV-2 virus SARS-CoV-2 virus infection scFv selected Spike protein subsequent the S1 subunit the spike protein therapeutic unique VeroE6 cells Viral viral infection viral spike protein virus virus infection [DOI] 10.1038/s41467-021-21609-2 PMC 바로가기 [Article Type] Article