SARS-CoV-2 S1 and N-based serological assays reveal rapid seroconversion and induction of specific antibody response in COVID-19 patientsArticle Published on 2020-10-062022-10-04 Journal: Scientific Reports [Category] MERS, SARS, 유전자 메커니즘, 치료기술, [키워드] Antibody Response caused collected Contact COVID-19 COVID-19 patient COVID-19 patients CoVs cross-reactivity cut off different time point disease onset ELISA Epidemiological study healthy controls human coronavirus IgG antibody Inclusion individuals Infection nucleocapsid populations positive RT-PCR SARS-CoV-2 SARS-CoV-2 S1 sensitivity Sensitivity and specificity sera Seroconversion Serological assay Serological testing seropositive specific IgM specificity spike glycoprotein Spread subunit tested the antibody response viral infection virus virus spread [DOI] 10.1038/s41598-020-73491-5 PMC 바로가기 [Article Type] Article
Multicenter evaluation of two chemiluminescence and three lateral flow immunoassays for the diagnosis of COVID-19 and assessment of antibody dynamic responses to SARS-CoV-2 in TaiwanResearch Article Published on 2020-10-012022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] 2019-nCoV 95% CI 95% confidence interval Abbott Abbott SARS-CoV-2 IgG acute respiratory syndrome antibody Antibody Response antibody tests autoantibodies Characteristics chemiluminescence chemiluminescence immunoassay chemiluminescence immunoassays CMV coronavirus coronavirus disease COVID-19 COVID-19 infection cross-reactivity demonstrated Diagnosis diagnosis of COVID-19 diagnostic evaluate FIVE IgG/IgM antibody Infection Interpretation lateral flow immunoassay Lateral flow immunoassays LFIAs multicenter non-COVID-19 patient Patient patient with COVID-19 patients with COVID-19 Pneumonia Rapid response Retrospective study risk Roche SARS-CoV-2 sensitivity sera Seroconversion seroconversion time serological serological test serum sample specificity suggested symptom onset Taiwan Test [DOI] 10.1080/22221751.2020.1825016 PMC 바로가기 [Article Type] Research Article
Persistence of Anti-SARS-CoV-2 Antibodies in Non-Hospitalized COVID-19 Convalescent Health Care WorkersArticle Published on 2020-10-012022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] addressed affected anti-SARS-CoV-2 antibodies Antibody Response Antibody titer Blood collected correlation COVID-19 COVID-19 infections ELISA assay expressing full length General population healthcare worker ICU IgA antibody IgG IgM immune response Infection intensive care unit magnitude Patient patients hospitalized persistence Prevalence pro-inflammatory cytokine pro-inflammatory mediator pro-inflammatory mediators profile Protein question RBD Re-infection SARS-CoV-2 SARS-COV-2 infection sera Serological testing short duration spike subject tested the antibody the N protein viral clearance Viral protein worker [DOI] 10.3390/jcm9103188 PMC 바로가기 [Article Type] Article
SARS-CoV-2 antibodies, serum inflammatory biomarkers and clinical severity of hospitalized COVID-19 patientsResearch article Published on 2020-10-012022-10-05 Journal: Journal of Clinical Virology [Category] SARS, 바이오마커, [키워드] analyzed anti-RBD antibody approaches association Blood C reactive protein Clinical data Clinical severity collected comparable Complete correlation COVID-19 COVID-19 patient COVID-19 severity CRP demographic different time point domain ELISA event exhibited ferritin GFP hospitalized COVID-19 patient hospitalized patient ICU ICU patient IgG IgGs IL-6 inflammatory biomarkers intensive care interleukin-6 investigated LDH Neutralizing antibodies neutralizing antibody non-ICU patient onset of symptom Patient platform pro-inflammatory biomarkers Protein Pseudotyped virus RBD recognizing Result retrieved retrospective SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 IgG SARS-CoV-2 RBD sera serum inflammatory biomarker Serum level suggested Support were measured [DOI] 10.1016/j.jcv.2020.104611 [Article Type] Research article
COVID-19 vaccine BNT162b1 elicits human antibody and TH1 T cell responses Research Published on 2020-09-302022-10-05 Journal: Nature [Category] 유전자 메커니즘, 임상, 치료기술, 치료제, [키워드] acute respiratory syndrome Adults age antibody Antibody Response BNT162b1 CD4+ CD4+ T cell CD4+ T cells CD8+ CD8+ T cell Cohort Concentration coronavirus disease Coronavirus-2 COVID-19 COVID-19 vaccine cytokine response dose effective elicit elicited ENCODE fraction geometric mean healthy immune response individual individuals mechanisms MOST mRNA vaccine neutralized non-randomized nucleoside-modified mRNA observer-blinded Open-label pandemic participant placebo-controlled produced PROTECT pseudovirus RBD RBD-binding IgG Receptor binding domain reported response responses robust SARS-CoV-2 SARS-CoV-2 spike variants sera serum Spread T cell T cell response T helper the SARS-CoV-2 titre Tolerability Trial vaccination Vaccine vaccine trial [DOI] 10.1038/s41586-020-2814-7 [Article Type] Research
S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 SubunitResearch Article Published on 2020-09-252022-10-29 Journal: mBio [Category] COVID-19, SARS, [키워드] accompanied acute respiratory syndrome Analysis analyzed anti-N IgG anti-S2 antibody antibody production asked B cell B cell response circulating conserved convalescent coronavirus coronavirus disease correlated strongly COVID-19 cross-reactive disease ENhance frequencies generate healthy donor Human human coronavirus OC43 IgG IgG antibodies IgG level IgG titer immune memory immune protection Immunity individuals Infection MBC memory B cell memory B cells mononuclear cell MOST non-SARS-CoV-2 protein nucleocapsid pandemic PBMCs Peripheral blood Population Postinfection Production Protein provide RBD reactive Receptor binding domain reflect Reinfection S2 subunit SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 protein SARS-CoV-2 proteins sera specificity Spike protein Spread subject subjects susceptibility susceptible the RBD the S protein the SARS-CoV-2 virus Virus-specific IgG [DOI] 10.1128/mBio.01991-20 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primatesResearch Article Published on 2020-09-242022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome aluminium adjuvant Analysis antigenicity appear candidate convalescent coronavirus coronavirus disease COVID-19 COVID-19 patient dose ectodomain elicit ELISA enzyme-linked immunosorbent evaluated expressed expression glycosylation highly pathogenic homotrimer immunization immunogenicity Inclusion induce insect cell insect cell expression system lowest mammalian cell mice Microscopy monomer neutralization non-human primate offer pandemic prefusion produced Protein RBD reactivity Receptor binding domain recombinant COVID-19 vaccine reduced regimen S protein S-2P S-protein S1 subunit SARS-CoV-2 SARS-CoV-2 spike sera spike subjects suggested the mean therapeutic titre Transmission trimer Vaccine wild-type [DOI] 10.1080/22221751.2020.1821583 PMC 바로가기 [Article Type] Research Article
Cross-Protection against MERS-CoV by Prime-Boost Vaccination Using Viral Spike DNA and ProteinVaccines and Antiviral Agents Published on 2020-09-232022-10-30 Journal: Journal of Virology [Category] COVID-19, [키워드] addition amino acids binding cause cell-mediated immune response Cellular immune response cellular receptor Combination Coronavirus vaccines Coronaviruses cross-neutralization cross-protection cross-protective demonstrated develop DNA DNA plasmid DNA viruses effective examined expected Heterologous high mortality human DPP4 humoral immunization immunized immunized mice induce interferon MERS-CoV mice Middle East mouse Mutation Neutralizing antibodies neutralizing antibody platform priming promote Protein provided RBD Recombinant protein regimen respiratory syndrome coronavirus RNA virus S-protein S-pseudovirion sequence sera severe respiratory illness spike spike glycoprotein strain subunit suggested the receptor-binding domain vaccination Vaccine Vaccine development Vaccines variant viral infection viral strain viruses wild-type [DOI] 10.1128/JVI.01176-20 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
Serological Tests for SARS-CoV-2 Coronavirus by Commercially Available Point-of-Care and Laboratory Diagnostics in Pre-COVID-19 Samples in JapanBrief Report Published on 2020-09-232022-11-01 Journal: Diseases [Category] COVID-19, [키워드] Combination COVID-19 COVID-19 patient diagnostics ELISA enzyme-linked immunosorbent European examined excluded False positive false positive rate healthy subject IgG investigated Japan Lateral less LFA Monte Carlo method performed Population positive prevalent reported Sample SARS-CoV-2 SARS-CoV-2 coronavirus sera serological survey serological test specificity Test unlikely [DOI] 10.3390/diseases8040036 PMC 바로가기 [Article Type] Brief Report
Subunit Nanovaccine with Potent Cellular and Mucosal Immunity for COVID-19COVID-19에 대한 강력한 세포 및 점막 면역을 가진 서브유닛 나노백신Article Published on 2020-09-212022-09-12 Journal: ACS applied bio materials [Category] MERS, SARS, 진단, [키워드] activating acute respiratory syndrome adjuvants alum adjuvant cationic CD4 CD8 cells cellular cellular immunity coronavirus COVID-19 CpG elicit humoral humoral immune response Humoral immunity IgA antibody Immunity infecting inhibit SARS-CoV-2 Liposome mice nanoadjuvant nanovaccine pandemic potent receptor SARS-CoV-2 sera significantly strong T-cell the S1 subunit Vero cells virus [DOI] 10.1021/acsabm.0c00668 PMC 바로가기 [Article Type] Article