An in vitro model for assessment of SARS-CoV-2 infectivity by defining the correlation between virus isolation and quantitative PCR value: isolation success of SARS-CoV-2 from oropharyngeal swabs correlates negatively with Cq value바이러스 분리와 정량적 PCR 값 간의 상관 관계를 정의하여 SARS-CoV-2 감염성을 평가하기 위한 시험관 내 모델: 구강 인두 면봉에서 SARS-CoV-2의 분리 성공은 Cq 값과 음의 상관 관계가 있습니다.Research Published on 2021-04-072022-09-01 Journal: Virology Journal [Category] MERS, SARS, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Applying calculated caused Cell Cell culture Chain Reaction coronavirus correlation COVID-19 patient Cq value decrease Diagnosis E-gene Epidemiological study experiment high risk highest in vitro in vitro model indication induce Infection infectious infective Infectivity inoculated Isolation Laboratory testing less long-lasting negative correlation novel Oropharyngeal oropharyngeal swab pandemic Pathogens Patient PCR polymerase chain polymerase chain reaction positive Probability Public public health qPCR Quantitation of viral infectivity Quantitative quantitative PCR quantitative polymerase chain reaction question raise respiratory Result retrospective risk factor risk of transmission RNA SARS-CoV-2 SARS-CoV2 severe acute respiratory syndrome Coronavirus significantly symptomatic Transmissibility Transmission Transmission pattern in vitro Viral Viral load viral pathogens virus virus isolation Virus titration [DOI] 10.1186/s12985-021-01542-y PMC 바로가기 [Article Type] Research
Identification of multipotent drugs for COVID-19 therapeutics with the evaluation of their SARS-CoV2 inhibitory activityResearch Article Published on 2021-04-072022-10-04 Journal: Computational and Structural Biotechnology Journal [Category] 신약개발, [키워드] Abstract Affect affected analyzed average bioinformatics cause Cell changed contagious coronavirus COVID-19 COVID-19 disease COVID-19 therapeutics disease manifestation docking drug Drug repurposing expression FDA approved drug help human host human protein IC50 identification identify in-silico inhibitory activity lung molecular dynamics network analysis nine pathogen Polypharmacology predicted molecule Protein Proteins SARS-CoV2 SARS-CoV2 infection targeting therapeutic option Treatment virus [DOI] 10.1016/j.csbj.2021.04.014 PMC 바로가기 [Article Type] Research Article
Cytotoxic T-lymphocyte elicited vaccine against SARS-CoV-2 employing immunoinformatics frameworkArticle Published on 2021-04-072022-10-04 Journal: Scientific Reports [Category] MERS, SARS, 신약개발, [키워드] acute respiratory syndrome Analysis assays binding binding interaction Biophysics caused Computational biology and bioinformatics coronavirus COVID-19 Critical CTL CTL epitope development effective elicit elicited epitope explicit fluctuation global pandemic Healthcare system Host immune regulators immune response immunization immunodominant immunoinformatics immunological parameter in silico in vitro in vivo information linker magnitude membrane memory cell MHC Molecular docking study Molecular dynamics simulation novel coronavirus disease predicted Protein receptors reduced Region residue RMSD robust SARS-CoV-2 SARS-CoV2 spike glycoprotein Strains T-cell TLR2 Vaccine vaccine candidate Variation [DOI] 10.1038/s41598-021-86986-6 PMC 바로가기 [Article Type] Article
Distinct SARS-CoV-2 antibody reactivity patterns in coronavirus convalescent plasma revealed by a coronavirus antigen microarray코로나바이러스 항원 마이크로어레이로 밝혀진 코로나바이러스 회복기 혈장의 뚜렷한 SARS-CoV-2 항체 반응성 패턴Article Published on 2021-04-062022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, [키워드] acute COVID-19 Adaptive immunity administration analyses Analysis analyzed antibody reactivity Antibody Response Antibody responses Antigen antigens array assays CCP chemiluminescence immunoassay clinical Clinical studies Cluster Clustering analysis collected Computational approaches Concordance convalescent plasma coronavirus COVID-19 case COVID-19 cases COVID-19 convalescent plasma Cutoff disease Donor Emergency use EUA FDA Generalized linear model Hierarchical Hierarchical clustering analysis Humoral immunity identify immunoassay Immunoglobulin IMPROVE Infection intensity MERS Microarray multiplex neutralization Neutralization assay offer plasma plasma sample plasma samples positive Predictive pseudovirus random Random forest reactive reactivity reactivity pattern recombinant proteins reporter Result SARS-1 SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-COV-2 infection SARS-CoV2 serum specimen the SARS-CoV-2 therapeutic efficacy threshold thresholds Total total Ig training set Treatment vaccination vaccine immunogenicity Viral viral particle was used with COVID-19 [DOI] 10.1038/s41598-021-87137-7 PMC 바로가기 [Article Type] Article
Adhatoda Vasica attenuates inflammatory and hypoxic responses in preclinical mouse models: potential for repurposing in COVID-19-like conditionsAdhatoda Vasica는 전임상 마우스 모델에서 염증 및 저산소 반응을 약화: COVID-19 유사 조건에서 용도 변경 가능성Research Published on 2021-04-062022-08-31 Journal: Respiratory Research [Category] MERS, 신약개발, 유전자 메커니즘, 진단, 치료제, [키워드] adaptive Adaptive immunity Adhatoda vasica Airway inflammation Angiogenesis anti-inflammatory effect Anti-inflammatory effects attenuate Bleomycin Blood coagulation cecum Coagulation condition conditions COVID-19 COVID-19 pneumonia downregulation Effect Extract feature fibrosis growth growth factor herbal medicine HIF-1α highlight hyperinflammation hypoxia hypoxic IL-6 IMPROVE Inflammation Inflammatory ligation lung mice molecular Mouse models murine model murine models naïve observation oral administration pathology pathway phenotype Pneumonia pulmonary fibrosis Puncture reduced Replication response Result SARS-CoV2 Sepsis siRNA supplementary material survival rate tested TGF-β1 thrombosis Transcriptome transforming growth factor treated Treatment upregulation Vero Cell Vero cells Viral Viral load [DOI] 10.1186/s12931-021-01698-9 PMC 바로가기 [Article Type] Research
Clinical characteristics and outcomes of COVID-19 patients with diabetes mellitus in KuwaitResearch Article Published on 2021-04-052022-10-31 Journal: Heliyon [Category] COVID-19, [키워드] Asymptomatic C-reactive protein Characteristics clinical Clinical characteristics Comorbidities Comorbidity complications contributing to COVID-19 COVID-19 patient COVID-19 patients CRP death diabete Diabetes Mellitus Diabetic disease severity fasting blood glomerular filtration rate Glucose had more hypertension ICU admission incidence increase in Inflammation Kuwait Laboratory laboratory investigation Logistic regression analysis management Mortality objective outcome pathophysiology Patient Prevalence recorded Result Retrospective study SARS-CoV2 severe respiratory symptom significantly higher single-center understanding worsening [DOI] 10.1016/j.heliyon.2021.e06706 PMC 바로가기 [Article Type] Research Article
Evaluation of the effectiveness and safety of adding ivermectin to treatment in severe COVID-19 patients중증 코로나19 환자 치료에 이버멕틴 추가의 유효성 및 안전성 평가Clinical Trial Published on 2021-04-042022-07-29 Journal: BMC Infectious Diseases [Category] 임상, 치료제, [키워드] added affecting Alter average Azithromycin azithromycin- blood lymphocyte count C-reactive protein can be used cause changes in Clinical improvement clinical recovery clinical response control group control groups COVID-19 COVID-19 disease COVID-19 pandemic COVID-19 pneumonia COVID19 pneumonia CRP D-dimer level D-dimer levels decrease drug Effect effective Effectiveness enteral use evaluate evaluated Favipiravir ferritin FiO2 follow-up period gene mutation gene mutations genetic test genetic tests groups Hydroxychloroquine increase in investigated Ivermectin ivermectin metabolism ivermectin treatment laboratory parameter Laboratory parameters lymphocyte material metabolism Mortality mortality rate mortality rates Mutation oxygen oxygen saturation PaO2 partial pressure Partial pressure of oxygen Patient patients performing sequence analysis peripheral capillary phase 3 study Pneumonia prognostic protocol protocols randomization Randomized reduction in reference treatment Result Sanger method SARS CoV-2 SARS-CoV2 Sequence analysis serum serum C-reactive protein severe COVID-19 severe COVID-19 patient Severe COVID-19 pneumonia single-blind solution SpO2 study group supplementary material the study group toxic effect toxic effects Treatment two groups were excluded were given while PMC 바로가기 [Article Type] Clinical Trial
Plasma biomarker profiling of PIMS-TS, COVID-19 and SARS-CoV2 seropositive children – a cross-sectional observational study from southern India.PIMS-TS, COVID-19 및 SARS-CoV2 혈청양성 아동의 혈장 바이오마커 프로파일링 - 인도 남부의 횡단면 관찰 연구.Observational Study Published on 2021-04-022022-08-31 Journal: EBioMedicine [Category] MERS, 바이오마커, 유전자 메커니즘, 임상, [키워드] acute COVID-19 Analysis Biomarker Care CCL11 CCL19 CCL2 CCL20 CCL5 chemokine chemokines Chennai children Children's Hospital clinical phenotype clinical phenotypes conditions controls correlated country COVID-19 COVID-19 infection cross-sectional CRP CXCL10 cytokine Cytokine storm Cytokines elevated finding GM-CSF granzyme granzyme B growth factor Growth factors hospital Hyperinflammatory IFNα IFNβ IFNγ IL-1 IL-10 IL-15 IL-17A IL-1α IL-2 IL-33 IL-6 immune India Infection Inflammatory lymphocyte Lymphocyte count management MIS-C multiplex negative correlation paediatric inflammatory multisystem syndrome Pathogenesis performed PIMS PIMS-TS plasma biomarker positive correlation presenting pro-inflammatory cytokines SARS-CoV-2 SARS-COV-2 infection SARS-CoV2 seropositive significantly sodium sodium levels syndrome TNFα trajectory unique VEGF [DOI] 10.1016/j.ebiom.2021.103317 PMC 바로가기 [Article Type] Observational Study
Tocilizumab treatment in critically ill patients with COVID-19: A retrospective observational study코로나19 중증 환자에 대한 토실리주맙 치료: 후향적 관찰 연구Observational Study Published on 2021-04-012022-09-12 Journal: International Journal of Infectious Diseases [Category] SARS, 임상, 진단, 치료법, [키워드] acute respiratory distress syndrome administration attenuate chimeric antigen receptor conducted COVID-19 COVID-19 patients Critically ill Critically ill patient Cytokine release syndrome Cytokine storm death disease severity distress dysregulated immune response group hospital hospital discharge intensive care interleukin-6 receptor Intubated invasive measurement mechanical ventilation not different on mechanical ventilation outcome Patient patients patients treated percentage Pneumonia pro-inflammatory cytokine progressed required Respiratory disease retrospective SARS-CoV2 Secondary infection severe COVID-19 infections subgroup of patient Tocilizumab treat Treatment venous [DOI] 10.1016/j.ijid.2021.02.057 PMC 바로가기 [Article Type] Observational Study
In-silico approaches to detect inhibitors of the human severe acute respiratory syndrome coronavirus envelope protein ion channel인간 중증 급성 호흡기 증후군 코로나바이러스 외피 단백질 이온 채널의 억제제를 검출하기 위한 인-실리코 접근법Article Published on 2021-04-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 치료기술, [키워드] Absorption acute respiratory syndrome amino acids approach binding caused computational approach Computational approaches coronavirus coronavirus disease Coronaviruses COVID-19 detect disease docking drug E protein envelope protein enveloped virus excretion help humans inhibit inhibitor Interaction ion channel metabolism modeling modulate molecular molecular dynamics outbreak pandemic Pathogenesis Protein quest random recent reduced reported Result rule SARS-CoV2 Severe acute respiratory syndrome Toxicity turn vertebrates virus α-helix [DOI] 10.1080/07391102.2020.1751300 PMC 바로가기 [Article Type] Article