UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteinsArticle Published on 2021-07-052022-10-29 Journal: Scientific Reports [Category] COVID-19, SARS, [키워드] acute respiratory syndrome Analysis cause Contaminated Contamination coronavirus demonstrated dose Effect electronic microscopy Evidence full-length Genome Health immunoblotting inactivated indicated mechanism Microbiology morphology negative correlation nucleocapsid protein Optics and photonics pandemic Protein R 2 reduced remained RT-PCR assay RT-qPCR SARS-CoV-2 Transmission Treatment ultraviolet C viral genome viral particle Viral proteins viral spike virus virus infectivity virus transmission [DOI] 10.1038/s41598-021-93231-7 PMC 바로가기 [Article Type] Article
Identification of a High-Frequency Intrahost SARS-CoV-2 Spike Variant with Enhanced Cytopathic and Fusogenic Effects세포변성 및 융합생성 효과가 강화된 고주파수 인트라호스트 SARS-CoV-2 스파이크 변이체 식별Research Article Published on 2021-06-292022-09-10 Journal: mBio [Category] MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amino acid amino acids analyzed coronavirus COVID-19 cytopathic effects data sets disease Diversity Effect enhanced Evolution exhibit exonuclease Factors Frequency gene encoding genetic diversity Genetic variant genetic variants High-throughput sequencing Host hosts identification infected cell infected individual infected individuals involved M protein Missense mutation molecular molecular evolution MOST Mutation Neutral produced Proofreading Protein Quasispecies Replication respiratory resulting RNA-dependent RNA polymerase S protein SARS-CoV-2 SARS-CoV-2 variants sequence sequencing data severe acute respiratory syndrome Coronavirus spike Spike protein Syncytia syncytium formation the S protein transmitted Variation variations Viral viral spike virus [DOI] 10.1128/mBio.00788-21 PMC 바로가기 [Article Type] Research Article
Development of SARS-CoV-2 Specific IgG and Virus-Neutralizing Antibodies after Infection with Variants of Concern or VaccinationArticle Published on 2021-06-252022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] anti-N IgG Anti-RBD IgG anti-S IgG AstraZeneca avidity B.1.1.7 B.1.351 BNT162b2 ChAdOx1 concern contribute convalescent COVID-19 cVNT demonstrated detectable development domain Evidence examined humoral immune response Humoral immunity IgG immune response immunoblot Immunoglobulin increase in Infection Maturity Moderna mRNA-1273 neutralization Pfizer/BioNTech RBD reactivity robust SARS-CoV-2 SARS-COV-2 infection second dose sera Specific starting sVNT tested titer vaccination vaccine dose vaccinee vaccinees variants of concern viral spike virus variants virus-neutralizing antibodies virus-neutralizing antibody VoC was determined were used [DOI] 10.3390/vaccines9070700 PMC 바로가기 [Article Type] Article
Structure-based Design of a Specific, Homogeneous Luminescence Enzyme Reporter Assay for SARS-CoV-2SARS-CoV-2에 대한 특정 균질 발광 효소 리포터 분석의 구조 기반 설계Article Published on 2021-06-252022-09-11 Journal: Journal of Molecular Biology [Category] MERS, SARS, 진단, 치료기술, [키워드] antibody applied assays binding C-terminal catalytic activity complementary Complementation COVID-19 detectable develop diagnostic enzyme epitope Epitopes Express Gaussia Luciferase high affinity Incubation luciferase other diseases pathogenic PCA promise Protein protein-fragment complementation assay recombinant recombinant antibodies reporter reporters S-protein SARS CoV SARS CoV-2 SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 virus sensitivity Specific specificity spike spike (S) protein steric the SARS-CoV-2 virus threat Treatment Trimers Viral viral spike Virus-like particle virus-like particles [DOI] 10.1016/j.jmb.2021.166983 PMC 바로가기 [Article Type] Article
Attractive and repulsive residue fragments at the interface of SARS-CoV-2 and hACE2Article Published on 2021-06-152022-10-29 Journal: Scientific Reports [Category] COVID-19, [키워드] addition antibody binding binding affinity Biophysics conformation Contact coronavirus cryo-EM Epitopes hACE2 human ACE2 receptor human cell identify include indicate initial Interaction intrinsically involved mechanism Microscopy molecular biophysics Molecular modelling Other promote Protein Receptor binding domain residue S-RBD SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 coronavirus SARS-CoV-2 spike protein Stage structural biology the SARS-CoV-2 van der Waal viral spike Virology X-ray crystallography [DOI] 10.1038/s41598-021-91877-x PMC 바로가기 [Article Type] Article
SARS-CoV-2, a Threat to Marine Mammals? A Study from Italian SeawatersArticle Published on 2021-06-032022-10-28 Journal: Animals : an Open Access Journal from MDPI [Category] COVID-19, [키워드] ACE-2 ACE-2 receptor affected Analysis angiotensin Animal host anticipated carried characterized consequence coronavirus coronavirus disease-2019 Critical death discharge evaluate event expression extreme functional immunohistochemistry interactions Italian Italian wastewater management species lung tissue main receptor mammal management marine mammals MERS-CoV Occurrence overlapped pandemic persistence Population populations Prevent Protein public health question raise raised receptor reported respiratory respiratory tracts responsible risk SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pandemic Seawater sequence homology simple specimens susceptibility susceptible the SARS-CoV-2 threat Transmission transmitted viral spike zoonotic transmission [DOI] 10.3390/ani11061663 PMC 바로가기 [Article Type] Article
Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 GenesArticle Published on 2021-05-312022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] antigen processing arising binding bioinformatics cell line Comparative complex complications of COVID-19 contribute COVID-19 pandemic Critical disease down-regulated down-regulated gene down-regulation Endoplasmic reticulum endoribonuclease (NSP15) enrichment analysis expression facilitate global public health heart development Heat shock Host host gene Human Immunity insulin signaling pathway Interaction methyltransferase Methyltransferase (NSP16) nervous system neurodegeneration neutrophil degranulation Nucleocapsid (NP) nucleocapsid protein offer oxidative phosphorylation pathway phosphatidylinositol 3-kinase positive Protein Regulation response RNA sequencing RNA-sequencing S1 subunit S2 subunit SARS-CoV-2 SARS-CoV-2 pathogenesis SARS-CoV-2 proteins SARS-CoV-2 virus Signaling signaling pathway spike Spike S1 Spike S2 Stress target host cell transcriptomic transcriptomic alteration up-regulated viral gene viral spike virus receptor [DOI] 10.3390/microorganisms9061193 PMC 바로가기 [Article Type] Article
Avian antibodies (IgY) targeting spike glycoprotein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inhibit receptor binding and viral replicationResearch Article Published on 2021-05-282022-10-28 Journal: PLoS ONE [Category] COVID-19, SARS, [키워드] ACE2 acute respiratory syndrome Angiotensin converting enzyme-2 animal model antibody applied binding binding interactions binding site carried caused Concentration coronavirus COVID-19 Critical Culture effective evaluated feasibility feasible global pandemic glycoprotein human Ace2 protein immunized Immunoglobulin in vitro Infection Infectious disease inhibit inhibited inhibiting initial intranasal intranasally mitigate mucosal pandemic peak concentration peaking produced Prophylaxis PROTECT Protective Protein raised receptor Receptor binding reducing replication of SARS-CoV-2 respiratory pathogen Result resulting SARS-CoV-2 shown significantly specificity spike glycoprotein spread of COVID-19 the SARS-CoV-2 virus titer Vaccine Vero Cell viral replication viral spike virulence virus [DOI] 10.1371/journal.pone.0252399 PMC 바로가기 [Article Type] Research Article
The Spike of Concern—The Novel Variants of SARS-CoV-2우려의 급증 - SARS-CoV-2의 새로운 변종Review Published on 2021-05-272022-09-10 Journal: Viruses [Category] SARS, 변종, [키워드] angiotensin angiotensin I angiotensin I converting enzyme 2 antibody B.1.1.28 B.1.1.298 B.1.1.7 B.1.351 B.1.427 B.1.429 B.1.617 B1.526 collected Contact contact with D614G mutation enzyme Evolution hallmark human Ace2 protein India K417 Marseille-4 mutagenic Mutation N-terminal domain Neutralising Antibodies neutralising antibody North novel NTD open P.1 P.2 pandemic promote Protein RBD receptor Receptor binding domain receptor complex residue SARS-CoV-2 sequence identity South Africa South America spike spike mutation spike protomer surface areas the RBD United Kingdom variant variants Viral viral spike viral spread while Wuhan [DOI] 10.3390/v13061002 PMC 바로가기 [Article Type] Review
Scrutinizing Coronaviruses Using Publicly Available Bioinformatic Tools: The Viral Structural Proteins as a Case StudyMolecular Biosciences Published on 2021-05-242022-10-31 Journal: Frontiers in Molecular Biosciences [Category] COVID-19, [키워드] Analysis antibody antiviral molecule assumed beta-coronavirus Bioinformatic cause caused contribute COVID-19 DeepView molecular graphics program disease Effect effort envelope protein glycoprotein immunomodulator impair knowledge life-threatening Lung infection macromolecule membrane protein MERS-CoV molecular nucleocapsid protein other organ pandemic Protein SARS-CoV SARS-CoV-2 Spike protein stimulate structural protein the SARS-CoV-2 virus tissues Vaccine development viral spike virus viruses [DOI] 10.3389/fmolb.2021.671923 PMC 바로가기 [Article Type] Molecular Biosciences