PLP2 of Mouse Hepatitis Virus A59 (MHV-A59) Targets TBK1 to Negatively Regulate Cellular Type I Interferon Signaling PathwayResearch Article Published on 2011-02-182022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Activation acute respiratory syndrome assays catalytic domain cells cellular complex conserved coronavirus coronavirus cytoplasm deubiquitinase domain dose-dependent manner DUB ENCODE Evidence experiment finding identification IFN IFN signaling IFN signaling pathway IFN transcription IFN-β in vitro inactivated inactive Infection inhibit inhibited innate immune system IRF3 MHV-A59 mouse mouse hepatitis virus nonstructural protein 3 Nsp3 nuclear translocation overexpression Papain-like protease phosphorylate PLP2 Prevent principal Proteins receptor reduced Regulatory Replication reporter shown Spread supported target TBK1 TNF TRAF3 Treatment Type type I interferon upstream [DOI] 10.1371/journal.pone.0017192 PMC 바로가기 [Article Type] Research Article
Mixed infections with Chlamydia and porcine epidemic diarrhea virus – a new in vitro model of chlamydial persistenceResearch Article Published on 2010-07-272022-10-28 Journal: BMC Microbiology [Category] Coronavirus, [키워드] Alter appear Cell cells Chlamydia Chlamydiaceae Chlamydiae chronic disease Co-infection Culture detected developmental elementary body epidemic diarrhea evidenced experiment experiments heterogenous homogenous hpi Human Hypothesis immunofluorescence in vitro in vitro model Inclusion induce Infection infections investigated mixed infection NO observation PEDV persistence produced Re-infection Result TEM transmission electron microscopy Vero Cell virus [DOI] 10.1186/1471-2180-10-201 PMC 바로가기 [Article Type] Research Article
Infidelity of SARS-CoV Nsp14-Exonuclease Mutant Virus Replication Is Revealed by Complete Genome SequencingResearch Article Published on 2010-05-062022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] accumulate acute respiratory syndrome Author bioinformatics clone compared competent Complete complex consensus genome coronavirus coronavirus exoribonuclease Coronaviruses Critical Culture decrease deep sequencing demonstrated Effect Evolution examined experiment Frequency full-length genome Genetic Genome genome replication genome sequence growth Impact in vitro inactivation increase in Infidelity lack less limit mechanism MOST murine hepatitis virus mutant mutant virus Mutation nonstructural protein nonstructural protein 14 nsp14 nsp14-ExoN Nsps passage Pathogenesis powerful tool quasispecy recognize Replication replication fidelity required resulting Revealed RNA genome RNA genomes RNA virus SARS-coronavirus SARS-CoV sequence stability the Coronaviridae to define total population unique viral growth viral replication viral RNA genome viral system virus virus wild-type SARS-CoV [DOI] 10.1371/journal.ppat.1000896 PMC 바로가기 [Article Type] Research Article
Human airway epithelial cell culture to identify new respiratory viruses: Coronavirus NL63 as a modelArticle Published on 2008-12-052022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] airway amplification amplify antibody approach approaches Cell culture cell line clinical samples coronavirus CPE, cytopathic effect Culture culture supernatant cultures Cytopathic effect described detect determine different time point epithelial evaluated experiment HAE HAE culture HAE, human airway epithelium HCoV-NL63 HCoV-NL63, human coronavirus NL63 Human identify Infection lack molecular NL63 nucleic acid pathogen Pathogens Propagation reagent reported respiratory pathogen respiratory viral respiratory virus SARS-CoV, severe acute respiratory syndrome coronavirus secretion sequence Sequence analysis TEM TEM, transmission electron microscopy Transcriptome transmission electron microscopy variety virus Whole transcriptome amplification WTA, whole transcriptome amplification [DOI] 10.1016/j.jviromet.2008.10.022 PMC 바로가기 [Article Type] Article
DetectiV: visualization, normalization and significance testing for pathogen-detection microarray dataSoftware Published on 2007-09-142022-11-01 Journal: Genome Biology [Category] Coronavirus, [키워드] Analysis bioinformatics dataset DNA microarray experiment lack Microarray Normalisation offer statistical software R Visualisation [DOI] 10.1186/gb-2007-8-9-r190 PMC 바로가기 [Article Type] Software
Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape MutantsResearch Article Published on 2006-07-042022-10-29 Journal: PLoS Medicine [Category] Coronavirus, [키워드] 2003 acute respiratory syndrome Administered affected against SARS-CoV Alter Amino acid anti-SARS-CoV antibody antibody antibody concentration Antigen antigens approach arising Bacteria binding blocked Blood breadth carried caused center China close contact close person-to-person contact Combination complementary Concentration Control convalescent SARS patient coronavirus Coverage CR3014 CR3022 died different disease disease severity dose Effect effective Epidemic Epidemics epitope escape experiment feasible ferret ferrets finding generate glycoprotein Guangdong Health healthy Hong Kong Human human cell human cells human macrophage Human monoclonal antibody humans immune Immune escape immunization immunize in vitro individual effects Infected infecting Infection infectious Infectious disease information interact Interaction International killing Laboratory less limit lung damage Lung pathology mAb mAbs measure monoclonal monoclonal antibody Mutation neutralization neutralized NO organism Patient pharyngeal Prevent prevented prevention producing Prophylaxis provided quarantine recognize recognizing reduce reduction repeated replicated required Research researcher residue responsible SARS epidemic SARS-CoV SARS-CoV infection screened shown sick Spread strain suppress synergistic synergistic fashion synergy tested the disease the receptor-binding domain Travel treat variant variants viral escape viral spread virus virus Virus neutralization virus strain virus-neutralizing viruses website Wikipedia World Health Organization [DOI] 10.1371/journal.pmed.0030237 PMC 바로가기 [Article Type] Research Article
Study on the resistance of severe acute respiratory syndrome-associated coronavirusArticle Published on 2005-03-202022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] addition Complete coronavirus demonstrated Disinfection E. coli Escherichia coli experiment found Free hospital in vitro inactivation PBS persistence residue resistance SARS-CoV sodium susceptible tap Urine virus [DOI] 10.1016/j.jviromet.2005.02.005 PMC 바로가기 [Article Type] Article
Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infectionsResearch Published on 2005-02-102022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Analysis binding Camelpox cell line cellular compared criteria cytomegalovirus determined experiment expression expression level Gene Human Infected infected cells Infection mRNA expression Protein quantitative real-time PCR ranking Real-time PCR reference reference gene reported SARS Coronavirus tested viral replication virus were infected yellow fever β-actin [DOI] 10.1186/1743-422X-2-7 PMC 바로가기 [Article Type] Research