The Upper Respiratory Tract of Felids Is Highly Susceptible to SARS-CoV-2 InfectionFelids의 상부 호흡기는 SARS-CoV-2 감염에 매우 취약합니다Article Published on 2021-09-302022-09-10 Journal: International Journal of Molecular Sciences [Category] SARS, 진단, 치료기술, [키워드] ACE2 ACE2 expression air–liquid interface Cell Cell culture cultures detection epithelial cell epithelial cells Ex vivo felines highlight Human Infection inoculated less lion lung Lungs nasal mucosa natural nose pathological changes predatory primary cell primary cell cultures question replicate Replication respiratory respiratory tract risk SARS-CoV-2 SARS-COV-2 infection Strong susceptibility susceptible tissue Trachea tracheal tract upper upper respiratory tract Viral viral replication virus transmission [DOI] 10.3390/ijms221910636 PMC 바로가기 [Article Type] Article
TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cellsArticle Published on 2021-09-232022-10-04 Journal: Cell Death & Disease [Category] 신약개발, [키워드] Activation anti-TNF Apoptosis autoinflammatory disease biochemical biopsy canonical CASP8 caspase caspase-8 cell death Cell death and immune response contribute Critical Crohn's disease Crohn’s disease cytokine death dependence driven by driver epithelial Ex vivo feature Genetic Gut Host IBD IEC IFN-γ immunopathology independent induce Inflammatory bowel disease inhibitor interferons intestinal epithelial cell Intestinal Organoids involved JAK inhibitor kill killing kinase loss-of-function mechanisms MLKL mucosal necroptosis Organoid pathway required retained sustained synergistic T cells therapeutic efficacy TNF-α TNFR1 Tumour-necrosis factors ZBP1 [DOI] 10.1038/s41419-021-04151-3 PMC 바로가기 [Article Type] Article
Inhibitory activity of FDA-approved drugs cetilistat, abiraterone, diiodohydroxyquinoline, bexarotene, remdesivir, and hydroxychloroquine on COVID-19 main protease and human ACE2 receptor: A comparative in silico approachResearch article Published on 2021-09-222022-10-05 Journal: Informatics in Medicine Unlocked [Category] 신약개발, 치료제, [키워드] ACE2 ACE2 receptor animal models claimed clinical trials COVID-19 Culture demonstrated drug drugs Dynamic simulation effective inhibitor Ex vivo FDA-approved drug host cell human ACE2 Hydroxychloroquine in silico Approach Infection inhibit inhibitor inhibitors investigated main protease MD simulation molecular molecular docking organ organoids Patient Potential treatment protease Protein Proteins Remdesivir respiratory virus SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 main protease specific treatment systemic effect tested the SARS-CoV-2 virus were used [DOI] 10.1016/j.imu.2021.100745 [Article Type] Research article
Ex vivo SARS-CoV-2 infection of human lung reveals heterogeneous host defense and therapeutic responses인간 폐의 생체 외 SARS-CoV-2 감염은 이질적인 숙주 방어 및 치료 반응을 나타냅니다Research Article Published on 2021-09-222022-09-10 Journal: JCI Insight [Category] SARS, 치료기술, 치료제, [키워드] animal models antiviral activity Biology bridge Cell cell line cell lines cell viability Cellular immune response Chloroquine clinical risk factor Clinical risk factors comparable COVID-19 COVID-19 infection COVID-19 pathogenesis cryobank CXCL8 Defense demonstrated detectable Dexamethasone Donor donors Drug screening Drug screens endothelial epithelial Ex vivo expression had no HCoV HCoV-OC43 heterogeneity heterogeneous heterogenous Host host response human infection human lung human lung tissue human SARS-CoV-2 IFNB1 IL6 Immune cell Infection Inflammation library mechanism metformin molecules N-hydroxycytidine OC43 Pathogens Population predicted preserved Remdesivir respiratory Respiratory pathogens reveal Sample SARS-CoV-2 SARS-COV-2 infection severe COVID-19 Sirolimus subset suppressed susceptibility susceptibility to infection therapeutic response tissue tissues viability Viral viral growth viral replication [DOI] 10.1172/jci.insight.148003 PMC 바로가기 [Article Type] Research Article
Co-Spray Dried Nafamostat Mesylate with Lecithin and Mannitol as Respirable Microparticles for Targeted Pulmonary Delivery: Pharmacokinetics and Lung Distribution in RatsArticle Published on 2021-09-192022-10-28 Journal: Pharmaceutics [Category] COVID-19, [키워드] acute respiratory syndrome Administered administration Analysis blood plasma Bronchoalveolar lavage fluid caused co-spray coronavirus Coronavirus disease 2019 COVID-19 distribution Dry powder inhaler Ex vivo in vitro in vivo inhibitor intratracheal administration intravenous lecithin lung lung tissue mannitol mesylate Microparticle nafamostat Nafamostat mesylate new strain pharmacokinetics pulmonary RAT respiratory tract SARS-CoV-2 SARS-CoV-2 S spray stability suppresse tissue transmembrane serine protease [DOI] 10.3390/pharmaceutics13091519 PMC 바로가기 [Article Type] Article
Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19광범위하게 지시된 SARS-CoV-2-특이적 CD4+ T 세포 반응에는 급성 및 소실된 COVID-19 환자의 막 및 핵단백질 내에서 자주 검출되는 펩티드 특이성이 포함됩니다.Research Article Published on 2021-09-162022-09-10 Journal: PLoS Pathogens [Category] SARS, 치료기술, 치료법, 치료제, [키워드] assays Author average binding affinity breadth CD4+ T cell Cohort cohort of patient COVID-19 cytokine data set defined dominant elicited ELISPOT ENCODE enzyme epitope Epitopes Ex vivo examined FIVE Frequency HLA HLA binding HLA class II ICS IFN-γ immune response immune responses immunological Immunopathogenesis in vitro include individual individual immune response interferon interferon-γ intracellular cytokine staining M protein membrane MHC class N protein novel nucleoprotein one patient overlapping Patient patients peptide peptides phenotype Protein resolved response response frequency SARS-CoV-2 envelope SARS-CoV-2 genome SARS-COV-2 infection screened Specificities specificity Spike protein structural protein structural proteins subsequent T cell T cell epitope T cell response T cell responses T cells tetramer the N protein the SARS-CoV-2 the spike protein to define Vaccine design Viral Viral proteins vitro cultivation [DOI] 10.1371/journal.ppat.1009842 PMC 바로가기 [Article Type] Research Article
Systemic Human Neutrophil Lipocalin Associates with Severe Acute Kidney Injury in SARS-CoV-2 PneumoniaArticle Published on 2021-09-142022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] 30-Day mortality Activation Acute kidney injury AKI analyzed Associate clinical Cohort Concentration COVID-19 COVID-19 patient CRRT cumulative risk Cutoff death dialysis dichotomized dimer dysfunction eight elevated Ex vivo greater HNL Human Increased increased risk intensive care invasive ventilation investigated Mild Mortality neutrophil Neutrophils nine organ dysfunction Patient PCR-confirmed performed Peripheral blood plasma progressed renal replacement therapy Respiratory failure risk ROC SARS-CoV-2 SARS-COV-2 infection shown suggested systemic systemically treated vasopressors Whole blood [DOI] 10.3390/jcm10184144 PMC 바로가기 [Article Type] Article
Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5Article Published on 2021-09-072022-10-04 Journal: Nature Communications [Category] 유전자 메커니즘, [키워드] Activation Calu-3 cell caused CCL5 change circulating clade Contact Ex vivo experiment functional Human IFN IFNB1 IFNL1 immune gene in vitro innate immune isolate Lineage lung cell MERS-CoV Middle East nosocomial outbreak onward transmission pandemic parental phenotypic Pneumonia receptor reduced remained Replication replicative fitness respiratory syndrome coronavirus reveal Saudi Arabia sequence Signaling significantly increased South Korea spread to Strains Surveillance viral epidemiology viral evolution Viral immune evasion virus viruses [DOI] 10.1038/s41467-021-25519-1 PMC 바로가기 [Article Type] Article
Differential plasmacytoid dendritic cell phenotype and type I Interferon response in asymptomatic and severe COVID-19 infection차등 혈장 세포질 수지상 세포 표현형 및 무증상 및 심한 COVID-19 감염에서 I 형 인터페론 반응Research Article Published on 2021-09-022022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, SARS, 유전자 메커니즘, [키워드] absence accompanied adaptive immune responses Anti-viral anti-viral IFN anti-viral response antiviral responses Asymptomatic asymptomatic SARS-CoV-2 infected subjects Cell cells cellular characterized chemokine chemokines circulating contribute coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pathogenesis COVID-19 patient Critically ill Critically ill patient critically ill patients cytokine Cytokine storm Cytokines death Defense dendritic cell dendritic cells disease severity early phase elevated ENhance epithelial cells event Evidence Ex vivo expression fine-tune Frequency hospitalized patient hospitalized patients Host host defense Host-directed therapies host-directed therapy human peripheral blood mononuclear cell IFN IFN-stimulated gene Immune cell immune cells immune responses Immunity IMPROVE in vitro induce Infection Inflammatory cytokine Inflammatory cytokines inflammatory phenotype inhibiting viral replication Innate innate immune response Innate immunity Interaction interferon interferons investigated low frequency Lung epithelial cells mechanism mononuclear cell multi-organ failure neuropilin observation Pathogenesis PBMC PD-L1 marker pDC/type I IFN axis Peripheral blood pharmacological strategy phenotype plasmacytoid dendritic cell plasmacytoid dendritic cells pro-inflammatory cytokine pro-inflammatory cytokines producing productive viral replication progression protective role regulate Regulation released responsible resulting SARS-CoV-2 SARS-CoV-2 infected subject SARS-COV-2 infection SARS-CoV-2 pandemic secreted secretion serum severe COVID-19 Severe COVID-19 Infection severe COVID-19 patients stratified the cytokine storm the interferon tissue damage TLR7/8 type I type I and III IFNs type I and III interferons Type I IFN type I IFN producing cells Viral viral replication Viral resistance virus viruses [DOI] 10.1371/journal.ppat.1009878 PMC 바로가기 [Article Type] Research Article
Human Kidney Spheroids and Monolayers Provide Insights into SARS-CoV-2 Renal Interactions인간 신장 회전 타원체 및 단층은 SARS-CoV-2 신장 상호 작용에 대한 통찰력을 제공합니다Article Published on 2021-09-012022-09-11 Journal: Journal of the American Society of Nephrology : JA [Category] SARS, 유전자 메커니즘, [키워드] ACE2 acute kidney Acute kidney injury acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 African green monkey AKI baseline level cause Cell cell death cellular proliferation clone Col1a1 Control coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Culture cultures cytopathic cytopathic damage Cytopathic effect dedifferentiation demonstrated disease Elevation epithelial cell epithelial cells Ex vivo exhibited fibrosis green monkey help higher infectivity Human human kidney human tubular kidney cells identity infect Injury insight Interaction interferon interferon pathway Intervention kidney Kidney disease kidney infection kidney spheroids kidney stem cells less LRP2 mechanisms monolayer monolayers morbidity outcome patients with COVID-19 presenting pulmonary involvement reduction in renal renal progenitors renal tubular renal tubular epithelial cells. Replication replication of SARS-CoV-2 SARS-CoV-2 severe acute respiratory syndrome Coronavirus SNAI2 Spheroid STAT6 Support Symptom the disease tubular cell Vero E6 viral entry viral infection virus with COVID-19 [DOI] 10.1681/ASN.2020111546 PMC 바로가기 [Article Type] Article