Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA ExtractionResearch Article Published on 2021-06-082022-10-27 Journal: BioMed Research International [Category] Coronavirus, COVID-19, MERS, SARS, [키워드] alcohol analysed assessment average checked Concentration Council COVID-19 determinant E gene E-gene evaluate extracted RNA extraction group ICMR Isopropyl molecular Nanodrop parameter pool positive protocol purification purified purified RNA qRT-PCR reagents real-time RT-PCR Research RNA RNA purification RNAseP RT-PCR SARS-CoV-2 Screening second set selected sensitivity Sensitivity and specificity significantly increased specificity specimen specimens spectrophotometer step suggested treated Treatment was tested [DOI] 10.1155/2021/6653950 PMC 바로가기 [Article Type] Research Article
Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection ManagementSARS-CoV-2의 식별 및 정량화를 위한 qRT-PCR, dPCR 및 바이러스 적정의 상관관계: 감염 관리를 위한 새로운 접근 방식Comparative Study Published on 2021-05-282022-09-01 Journal: Viruses [Category] SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus analytical methods assays can not causative agent cell cultures Chain Reaction coronavirus coronavirus disease Coronavirus disease 2019 correlation COVID-19 CT Ct value Culture diagnostic diagnostic routine diagnostics dose dPCR Endpoint extraction gold gold standard identification infecting infectious methodological New pandemic polymerase chain polymerase chain reaction protocol provide purification qRT-PCR Research respiratory resulting RNA RNA copies RNA copy RNA extraction SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specimen TCID50/mL tissue tissue culture titration to define Viral viral burden Viral RNA viral titration Wuhan Wuhan, China [DOI] 10.3390/v13061022 PMC 바로가기 [Article Type] Comparative Study
Efficacy and safety of Xuanfei Baidu granules for treating COVID-19 A protocol for systematic review and meta-analysis코로나19 치료를 위한 Xuanfei Baidu 과립의 효능 및 안전성 체계적인 검토 및 메타 분석을 위한 프로토콜 A4400 Published on 2021-05-212022-08-31 Journal: Medicine [Category] meta-analysis, SARS, 임상, 치료제, [키워드] Analysis average Baidu bias risk carried checked China Chinese clinical symptom Computed tomography Corona corona virus disease 2019 country COVID-19 COVID-19 diagnosis COVID-19 patient database Diagnosis disease effective Efficacy and safety evaluate evaluated evaluator Evidence extraction Granule Health Hospital stay include Infrastructure Intervention journal knowledge list lung management Meta Meta-analysis Negative conversion nucleic acid plan prevalent protocol Randomized controlled trials registration number registry released responsible review Science searched statistical systematic review technology Traditional Chinese medicine treating COVID-19 Treatment treatment of COVID-19 Trial Virus Disease Web website Wuhan Wuhan, China Xuanfei Baidu granule [DOI] 10.1097/MD.0000000000025653 PMC 바로가기 [Article Type] 4400
SalivaSTAT: Direct-PCR and Pooling of Saliva Samples Collected in Healthcare and Community Setting for SARS-CoV-2 Mass SurveillanceArticle Published on 2021-05-192022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] alleviate CDC comparable COVID-19 diagnostic evaluated extraction extraction-free FDA homogenization limitation limitations mass media PerkinElmer pooling positive protocol quantification RT-PCR RT-PCR assay RT-PCR kits RT-PCR test Saliva saliva sample Sample SARS-CoV-2 setting stages tested Treatment widespread [DOI] 10.3390/diagnostics11050904 PMC 바로가기 [Article Type] Article
Assessment of 12 qualitative RT-PCR commercial kits for the detection of SARS-CoV-2SARS-CoV-2 검출을 위한 12개의 정성적 RT-PCR 상용 키트 평가Article Published on 2021-05-012022-09-11 Journal: Journal of medical virology [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 assessment BGI can be used carried Chain Reaction change clinical sample comparable coronavirus COVID-19 Ct value Ct values Cycling Diagnosis extraction gene target in vitro in vitro diagnostics indicate individual Interpretation less Lyra MONITOR nCoV nCOV-2019 Novel coronavirus oligonucleotide patients with SARS-CoV-2 polymerase chain reaction protocol Public public health emergency real-time reverse transcriptase-polymerase chain reaction recommendations reverse transcriptase RNA RT-PCR RT-PCR kit RT-PCR kits SARS-CoV-2 scored selected sensitivity severe acute respiratory syndrome Coronavirus specificity specimen target tested thermocycler virus [DOI] 10.1002/jmv.26900 PMC 바로가기 [Article Type] Article
Will the extraction of COVID-19 from wastewater help flatten the curve?폐수에서 COVID-19를 추출하면 곡선을 평평하게 만드는 데 도움이 될까요?Article Published on 2021-05-012022-09-11 Journal: Chemosphere [Category] SARS, 치료기술, [키워드] absence And disadvantages approach Blancmange challenges complex coronavirus country COVID-19 determine Disadvantages distributions Efficiency excreta expel extraction Extraction method feces help hotspot humans hygiene identify lack Limited lockdown Log-normal distribution mathematical methodology movement Normal distribution Occurrence persistence quantification recommendation Research resulting Sanitation SARS-CoV-2 sewage suggested temperature Transmission Treatment Viral load waste wastewater Wastewater treatment widespread [DOI] 10.1016/j.chemosphere.2020.129429 PMC 바로가기 [Article Type] Article
Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCRSARS-CoV-2 RT-PCR에 대한 풀링된 비인두 또는 타액 표본에 대한 자동화된 고처리량 액체 기반 RNA 추출 플랫폼 평가Article Published on 2021-04-022022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] automated comparable COVID-19 COVID-19 pandemic Ct values detect diagnostics Efficiency extraction GAPDH inhibitors Large-scale testing liquid-based nucleic acid extraction magnetic bead Mass screening Nasopharyngeal swab no significant differences nucleic acid nucleic acid extraction Oropharyngeal paucity of data platform pooled specimens positive positive rate real-time RT-PCR RNA RNA extraction RT-PCR Saliva saliva specimen saliva specimens SAR-CoV-2 SARS-CoV-2 SARS-CoV-2 RT-PCR significant difference significant differences specimen specimens the SARS-CoV-2 was performed was used [DOI] 10.3390/v13040615 PMC 바로가기 [Article Type] Article
Pediatric dental emergency management and parental treatment preferences during COVID-19 pandemic as compared to 2019Research article Published on 2021-04-012022-10-05 Journal: Saudi Journal of Biological Sciences [Category] 치료법, [키워드] aerosol bivariate analysis children comparable COVID-19 COVID-19 pandemic Dental emergency Emergency extraction infected with COVID-19 investigated lockdown management pandemic parental pediatric Pediatric patient performed Precaution predominant preference provided Retrospective study risk of COVID-19 south India statistical significance Total treated Treatment was performed worsened [DOI] 10.1016/j.sjbs.2021.02.002 [Article Type] Research article
Rare SARS-CoV-2 antibody development in cancer patients암 환자에서 희귀 SARS-CoV-2 항체 개발Article Published on 2021-04-012022-09-11 Journal: Seminars in oncology [Category] MERS, SARS, 진단, 치료기술, [키워드] adaptive analyzed antibody antibody concentration assumed bead BGI Cancer cancer patient CAST Course COVID-19 develop Diagnosis doubt extraction humoral Hypothesis Immunity immunoassay individual infected with SARS-CoV-2 knowledge MERS-CoV multiple testing other coronaviruses pandemic Pathogenesis Patient positive provided qPCR Roche RT-qPCR SARS-CoV SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV2 SARS-CoV2 antibody development. SARS-CoV2 infection subsequent understanding Vaccine development were used [DOI] 10.1053/j.seminoncol.2020.12.003 PMC 바로가기 [Article Type] Article
Association Between Renin-Angiotensin-Aldosterone System Inhibitors and Clinical Outcomes in Patients With COVID-19 A Systematic Review and Meta-analysisResearch Published on 2021-03-312022-10-29 Journal: JAMA Network Open [Category] COVID-19, SARS, [키워드] 95% CI ACEi ACEIs adjusted adjusted analyses Adjusted analysis Admission AEs angiotensin receptor blocker angiotensin-converting enzyme inhibitor ARB ARBs article articles assisted ventilation association assumed benefit Case series case-control studies clinical Clinical outcome clinical outcomes Clinicians Cohort studies Complication contrary coronavirus disease covariates COVID-19 cross-sectional database decrease defined diagnosed duplicates eligible evaluated exacerbate extraction finding Future higher risk hypertension Importance increased risk indicated intensive care Laboratory measure Meta-analysis Mortality multiple comorbidities objective Odds ratio outcome Patient patients with COVID-19 Point Protective radiological random-effects model randomized clinical trial receive receiving recommendation reductions in Relevance Result review risk risk of death screened searched Selection severe adverse event severe AE significant decrease significantly lower Source Study design subgroup analysis subgroup of patient synthesis System systematic review total patient total patients Treatment was used were assessed with COVID-19 [DOI] 10.1001/jamanetworkopen.2021.3594 PMC 바로가기 [Article Type] Research