A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challengeResearch article Published on 2021-11-242022-10-05 Journal: Cell Reports [Category] SARS, 비임상, [키워드] Administered candidate vaccine cGAMP disease elicit ENhance expected flagellin form generate human ACE2 Humoral immunity immune response Immunity immunized Inactivated vaccine induce Infection intranasal route local immune response mice mucosal mucosal immunity nucleocapsid Prevent preventing disease protective efficacy protective immune response respiratory tract robust S protein SARS-CoV-2 SFLmut the N protein the S protein transgenic mice upper respiratory tract URT Vaccine viral clearance viral infection [DOI] 10.1016/j.celrep.2021.110112 [Article Type] Research article
ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injuryACE2-유사 카르복시펩티다제 B38-CAP는 SARS-CoV-2로 유발된 폐 손상으로부터 보호합니다Article Published on 2021-11-232022-09-12 Journal: Nature Communications [Category] MERS, SARS, 유전자 메커니즘, 진단, [키워드] ACE2 ACE2 expression acting acute lung injury alleviate angiotensin Angiotensin II Angiotensin-converting enzyme angiotensin-converting enzyme 2 animal models carboxypeptidase cardiovascular disease cell entry COVID-19 patients decoy degrade downregulate downregulated edema Elevation endogenous enzymatic activity enzyme Evidence human ACE2 IMPROVE in vivo Infection inhibit inhibit SARS-CoV-2 lung lung edema Lung injury Lung pathology Lungs not neutralize Pathologies pathology peptides PROTECT Protective Protein receptor recombinant Respiratory distress syndrome SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected hamster SARS-CoV-2-infected hamsters significantly soluble ACE2 spike Symptom therapeutic strategy transgenic mice Treatment virus [DOI] 10.1038/s41467-021-27097-8 PMC 바로가기 [Article Type] Article
Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization AssaysSARS-CoV-2에 대한 체액 면역 평가: 플라크 감소 중화 테스트 및 기존 및 대리 중화 분석의 다중 실험실 비교 검증Research Article Published on 2021-11-172022-09-12 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, [키워드] addition antibody antibody tests approval assays characterized conducted Conventional COVID-19 COVID-19 vaccines death demonstrated ELISA ENhance enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay evaluate Evaluating flow cytometry Health Organization human ACE2 human ACE2 receptor humoral Humoral immunity immune responses Immunity immunoserology include laboratory-confirmed Laboratory-confirmed COVID-19 lentiviral luciferase multitude natural immunity neutralization neutralization test neutralization tests Neutralizing Neutralizing antibodies Neutralizing antibody response offer outlined Patient plaque-reduction neutralization test platform Precision PRNT Protective protective immune response protective immunity pseudotype quantification reference standard SARS-CoV-2 SARS-CoV-2 PRNT SARS-CoV-2-neutralizing antibodies serological Serological assay specificity specimen surrogate Test Vaccine validation World Health Organization [DOI] 10.1128/Spectrum.00886-21 PMC 바로가기 [Article Type] Research Article
Rapid Assessment of Binding Affinity of SARS-COV-2 Spike Protein to the Human Angiotensin-Converting Enzyme 2 Receptor and to Neutralizing Biomolecules Based on Computer SimulationsImmunology Published on 2021-11-112022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] Affect affinity antibody approach assessment bind binding affinities binding affinity Biomolecule can be used cause change circulating computer Coronavirus disease 2019 COVID-19 domain Dynamics Effect experimental work expression facilitate hACE2 hACE2 receptor helping host cell membrane hotspot Human human ACE2 human Angiotensin-converting enzyme infect Infection modulate molecular dynamics mutated Mutation mutations Neutralizing Neutralizing antibodies protein-protein interaction protocol Rapid S-RBD S1 domain S2 domain SARS-CoV-2 SARS-CoV-2 genome Screening selected Simulation spike spike glycoprotein Spike protein Spike-RBD the binding affinity time-consuming vaccination variant virus [DOI] 10.3389/fimmu.2021.730099 PMC 바로가기 [Article Type] Immunology
Enhanced protective immunity against SARS-CoV-2 elicited by a VSV vector expressing a chimeric spike protein키메라 스파이크 단백질을 발현하는 VSV 벡터에 의해 유발되는 SARS-CoV-2에 대한 보호 면역 강화Article Published on 2021-11-102022-09-12 Journal: Signal Transduction and Targeted Therapy [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 administration Antigen cellular receptor Chimera chimeric coronavirus COVID-19 vaccines cross-neutralizing cross-reactivity dose elicit elicited enhanced expressing Genetic glycoprotein hACE2 Human human ACE2 humoral immune response immunization immunized immunized mice indicate intramuscular intranasal lesion Lungs macaque macaques mice Mouse models NAb nAb response NAbs neutralizing antibody protective immunity RBD receiving Receptor binding domain replacing rVSV-SARS-CoV-2 S protein Safe SARS-CoV SARS-CoV S SARS-CoV-2 SARS-CoV-2 RBD sera serum significantly significantly increased similarity single dose spike Spike protein spikes the S protein the SARS-CoV-2 titers transplantation Vaccines viruses VSV wild-type [DOI] 10.1038/s41392-021-00797-9 PMC 바로가기 [Article Type] Article
Mutation Y453F in the spike protein of SARS-CoV-2 enhances interaction with the mink ACE2 receptor for host adaptionSARS-CoV-2 스파이크 단백질의 돌연변이 Y453F는 숙주 적응을 위해 밍크 ACE2 수용체와의 상호 작용을 향상시킵니다Research Article Published on 2021-11-082022-09-10 Journal: PLoS Pathogens [Category] MERS, SARS, 변종, [키워드] ACE2 ACE2 receptor Active surveillance adaptive addition Amino acid amino acid change Analysis binding circulation Cluster convalescent sera convalescent serum COVID-19 patient COVID-19 patients demonstrated develop domain employee ENhance Evolution exhibited functional Genetic variant Host human ACE2 human ACE2 receptor humans identify infected with SARS-CoV-2 initial Interaction less MONITOR Mutation Netherland Netherlands ortholog outbreak Outbreaks position Prevent RBD domain receptor reservoirs risk SARS-CoV-2 SARS-CoV-2 evolution SARS-CoV-2 RBD sequence similarity Spike protein steric Structural analysis suggested susceptible susceptible hosts the RBD the spike protein therapeutic strategy Transmission transmitted Vaccine development Viral viral RBD virus widespread Y453F zoonotic [DOI] 10.1371/journal.ppat.1010053 PMC 바로가기 [Article Type] Research Article
In silico binding profile characterization of SARS-CoV-2 spike protein and its mutants bound to human ACE2 receptorSARS-CoV-2 스파이크 단백질 및 인간 ACE2 수용체에 결합된 돌연변이체의 인 실리코(in silico) 결합 프로필 특성화Article Published on 2021-11-052022-09-11 Journal: Briefings in Bioinformatics [Category] MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affected approach binding binding affinities binding affinity binding free energies binding free energy complexes coronavirus country COVID-19 decomposition domain drug resistance free energies free energy Free energy calculation and decomposition Free energy calculations G476S hACE2 hACE2 protein hotspot Hotspot residues human ACE2 human ACE2 receptor infect interfere investigated MD simulation molecular molecular dynamics Molecular mechanics mutant nine Novel coronavirus pandemic Poisson Protein–protein interaction protocol prototype provide RBD RBD mutations RBDs receptor Receptor-binding domain respiratory SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 Spike RBD severe acute respiratory syndrome Coronavirus small molecule small molecule drug small molecule drugs spike spike glycoprotein Spike RBD/hACE2. Structure structures surface area V367F V483A Vaccine virus [DOI] 10.1093/bib/bbab188 PMC 바로가기 [Article Type] Article
Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants새로 출현한 돌연변이 변이체에 대한 보호 효능이 있는 강력한 SARS-CoV-2 중화 항체Article Published on 2021-11-022022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] antibodies antibody authentic SARS-CoV-2 virus B.1.351 bind candidate Candidates COVID-19 COVID-19 pandemic cryo-EM E484K mutation Efficacy evidenced expressing hACE2 human ACE2 Immune escape in vitro in vivo mAbs mechanism monoclonal antibodies monoclonal antibody Mutation Neutralizing Neutralizing antibodies overlapping potent Prophylactic Prophylaxis Protective Protein RBD Receptor binding domain recognize reducing SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 mutants SARS-CoV-2 neutralizing antibody SARS-CoV-2 spike SARS-CoV-2 virus spike steric Structural analysis the RBD the SARS-CoV-2 transgenic mice treat unique variant virus virus infection viruses weight loss [DOI] 10.1038/s41467-021-26539-7 PMC 바로가기 [Article Type] Article
Effect of mutation on structure, function and dynamics of receptor binding domain of human SARS-CoV-2 with host cell receptor ACE2: a molecular dynamics simulations study돌연변이가 숙주 세포 수용체 ACE2와 인간 SARS-CoV-2의 수용체 결합 도메인의 구조, 기능 및 역학에 미치는 영향: 분자 역학 시뮬레이션 연구Article Published on 2021-11-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 변종, [키워드] 6M0J ACE2 ACE2-RBD Affect Alanine alanine mutant alanine mutants Analysis angiotensin angiotensin-converting enzyme-II binding binding affinity cellular protease cellular proteases complex complexes conserved coronavirus Critical Directed mutagenesis disease severity drug Effect entry of SARS-CoV-2 enzyme exhibit FIVE flexible host cell host cell receptor human ACE2 human SARS-CoV-2 hydrogen Infection information inhibitor initiate Interface residues intermolecular hydrogen bond MD simulation MD simulations molecular molecular dynamics Molecular dynamics simulation molecular interaction motif Mutagenesis mutant Mutation priming Principal component provide RBD RBM recent Receptor binding Receptor binding domain receptor binding motif receptor binding motif. Receptor-binding domain Replication rate residue residues SARS-CoV SARS-CoV-2 SARS-CoV-2 RBD Spike protein supported the binding affinity the receptor-binding domain viral spike protein ward wild-type [DOI] 10.1080/07391102.2020.1802348 PMC 바로가기 [Article Type] Article
Whey-Derived Peptides at the Heart of the COVID-19 PandemicCOVID-19 팬데믹의 중심에 있는 유청 유래 펩타이드Article Published on 2021-10-282022-09-10 Journal: International Journal of Molecular Sciences [Category] 치료제, [키워드] ACE ACE inhibitor ACE inhibitors ACE inhibitory activity ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 approach association blood pressure blood pressure homeostasis Captopril cells component coronavirus COVID-19 COVID-19 pandemic drugs dual enzyme exhibiting heart human ACE2 hypertension inhibit inhibitory inhibitory action inhibitory activity IPP Lisinopril molecular docking molecular interaction peptide peptide sequences peptides Previous studies produced RAS RAS inhibitor RAS inhibitors receptor reduced risk renin–angiotensin system renin–angiotensin system reported respiratory SARS-CoV-2 sequence severe acute respiratory syndrome Coronavirus shown synthetic drugs therapeutic Treatment treatment of COVID-19 were assessed whey peptides [DOI] 10.3390/ijms222111662 PMC 바로가기 [Article Type] Article