Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum AdjuvantsImmunology Published on 2022-01-122022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, [키워드] acute respiratory syndrome adjuvant adjuvants Administered alum alum adjuvant antigen-specific antibody approach cartilage coronavirus coronavirus disease COVID-19 COVID-19 pandemic effective Effectiveness efficient Euglena evaluated examined expression expression vector highlight host cells induce induction Infection inoculation mice motif mouse serum neutralizing antibody optimization paramylon produced Protein purified S protein SARS-CoV-2 secreted serum Silkworm-baculovirus expression vector system spike spike (S) protein Spread subunit the S protein therapeutic agents trimeric Vaccine Vaccine development was used [DOI] 10.3389/fimmu.2021.803647 PMC 바로가기 [Article Type] Immunology
Distinctive Biomarker Features in the Endotheliopathy of COVID-19 and Septic SyndromesCOVID-19 및 패혈성 증후군의 내피병증에서의 독특한 바이오마커 특징Comparative Study Published on 2022-01-012022-09-11 Journal: Shock (Augusta, Ga.) [Category] 바이오마커, [키워드] Activation ADAMTS adhesion analyzed Biomarker Biomarkers both groups circulating clinical disease collected complement Complement activation contributing to Control COVID-19 COVID-19 disease COVID-19 disease severity COVID-19 group COVID-19 patient COVID-19 patients COVID-19 phenotype COVID-19 pneumonia Critical critical COVID-19 patient critical COVID-19 patients Critically ill critically ill COVID-19 patients Culture disease disease severity dsDNA dysregulation e parameters endothelial Endothelial cell endothelial cells endothelial damage endotheliopathy except for Factor fibrinolysis fibrinolytic healthy donor healthy donors help heparan sulfate Hospitalization ICU in both groups increased expression information intensive care life-threatening mitigate moderate morbidity and mortality motif NETs neutrophil Neutrophil extracellular trap Neutrophil extracellular traps parameter pathophysiology Patient Phenotypes plasma samples Pneumonia progression receptor Result Sepsis sepsis syndrome septic Septic shock severity of COVID-19 significantly significantly higher significantly lower SIRS Stage Stress sulfate syndrome systemic inflammatory response systemic inflammatory response syndrome therapeutic strategy thrombomodulin TNF volunteers von Willebrand factor vWF was related with COVID-19 [DOI] 10.1097/SHK.0000000000001823 PMC 바로가기 [Article Type] Comparative Study
Deep mutational engineering of broadly-neutralizing nanobodies accommodating SARS-CoV-1 and 2 antigenic driftSARS-CoV-1 및 2 항원 드리프트를 수용하는 광범위 중화 나노바디의 심층 돌연변이 공학Article Published on 2022-01-012022-09-11 Journal: mAbs [Category] SARS, 변종, 진단, [키워드] ACE2 amino acids antibody antibody engineering antigenic applied binding contribute cross-reactivity deep deep mutational scanning Efficiency epitope high affinity immunodominant Interaction molecular motif nanobodies nanobody neutralize Neutralizing Pathogens RBD Receptor-binding domain Recognition regions sarbecovirus SARS-CoV-1 SARS-CoV-1 and 2 SARS-CoV-1 RBD SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-CoV-2 RBD screened specificity Substitution variant VHH72 virus yeast yeast surface display. [DOI] 10.1080/19420862.2022.2076775 PMC 바로가기 [Article Type] Article
Intra-Host SARS-CoV-2 Evolution in the Gut of Mucosally-Infected Chlorocebus aethiops (African Green Monkeys)점막에 감염된 클로로세 부스 아티 옵스 (African Green Monkeys)의 장에서 호스트 내 SARS-COV-2 진화Brief Report Published on 2022-01-012022-09-10 Journal: Viruses [Category] 변종, 치료기술, [키워드] African African green monkey (Chlorocebus aethiops) African green monkeys Amino acid amino acid change Cell culture Chlorocebus collected D215G ENhance Evolution furin furin cleavage site green green monkeys H655Y Host human disease Humoral immunity identify in viral in vivo motif Mutation ORF rectal swab Rectal swabs respiratory RNA SARS-CoV-2 SARS-CoV-2 evolution SARS-COV-2 infection SARS-CoV-2 variant SARS-CoV-2 variants sequenced therapy trajectory Transmissibility Vaccine variant of SARS-CoV-2 Viral viral evolution viral infection Viral RNA virus [DOI] 10.3390/v14010077 PMC 바로가기 [Article Type] Brief Report
Analysis of TCR Repertoire by High-Throughput Sequencing Indicates the Feature of T Cell Immune Response after SARS-CoV-2 InfectionArticle Published on 2021-12-272022-10-31 Journal: Cells [Category] COVID-19, SARS, [키워드] analyzed caused CD4 CD8 CDR3 characteristic clearance clone Clustering Combination contributing to convalescent convalescent COVID-19 patients coronavirus Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients dominant donors feature Frequency group healthy control immune memory immune response Infection Infectious disease Invasion J gene motif provide Recombination repertoire response SARS-CoV-2 SARS-CoV-2 antigen sequence Sequencing Specificities stages T cell T cell receptor T cell response TCR the healthy the SARS-CoV-2 virus virus recognition [DOI] 10.3390/cells11010068 PMC 바로가기 [Article Type] Article
Enhanced replication fitness of MERS-CoV clade B over clade A strains in camelids explains the dominance of clade B strains in the Arabian PeninsulaCoronaviruses Published on 2021-12-172022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, [키워드] adaptive alpaca Amino acid amino acid substitution Analysis animal Antiviral Arabian Peninsula binding caused clade decrease difference double-stranded elicited enhanced Epidemic explain Frame hosts Human Hypothesis IFN IFN response immunological parameters in vivo infecting Infection inoculated interferon-stimulated gene ISGs MERS-CoV Middle East Middle East respiratory syndrome Coronavirus moderate motif nasal nasal mucosa orf4a post inoculation Protein Replication replicative reported respiratory syndrome coronavirus respiratory tract RNA S proteins strain subsequent Transcription Transmission variant viral loads viral shedding Virological virus while [DOI] 10.1080/22221751.2021.2019559 PMC 바로가기 [Article Type] Coronaviruses
Molecular Characterization of Infectious Bronchitis Virus Strain HH06 Isolated in a Poultry Farm in Northeastern ChinaVeterinary Science Published on 2021-12-162022-11-01 Journal: Frontiers in Veterinary Science [Category] COVID-19, [키워드] Analysis bovine characterization characterized China clade clades coronavirus Coronaviruses CoV CoVs domain Epidemic Evolution explain farm Genetic Genome glycoprotein HCoV-OC43 Host IBV indicated Infectious Bronchitis Virus Isolated maintain mammalian MERS-CoV Molecular epidemiology motif Phylogenetic Phylogenetic relationship poultry protein sequence Recombination S gene S protein SARS CoV-2 SARS-CoV SARS-CoV-2 selected selection pressure sequence spike Spread strain variant virulent viruses zoonosis [DOI] 10.3389/fvets.2021.794228 PMC 바로가기 [Article Type] Veterinary Science
Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallographyArticle Published on 2021-12-162022-10-04 Journal: Nature Communications [Category] SARS, 변종, 치료제, [키워드] Analysis antibodies antibody Antibody fragment therapy Antibody fragments Antigen binding domain biochemical biologic classical coronavirus Critical delta variant effective epitope exceeded flexible full-length greater highlight identify immune system Immunoglobulin inaccessible lineages live SARS-CoV-2 mechanism motif neutralize New Phylogenetic Protein Protein design pseudotype RBD receptor Receptor binding domain recognize SARS-CoV-2 SARS-CoV-2 mutants SARS-CoV-2 neutralization SARS-CoV2 SARSCoV-2 screened shark the RBD the SARS-CoV-2 therapeutic agent unique utility variable viral neutralization virus Virus neutralization X-ray crystallography [DOI] 10.1038/s41467-021-27611-y PMC 바로가기 [Article Type] Article
The fatty acid site is coupled to functional motifs in the SARS-CoV-2 spike protein and modulates spike allosteric behaviourCommunications Published on 2021-12-112022-10-31 Journal: Computational and Structural Biotechnology Journal [Category] COVID-19, [키워드] Abstract ACE2, angiotensin-converting 2 enzyme Affect Alter binding binding pocket binding site CD, connector domain CH, central helix change conformational Contact D614G FA, fatty acid fatty FP, fusion peptide FPPR, fusion-peptide proximal region functional furin cleavage site Genetic variation host cell HR1, heptad repeat 1 human ACE2 receptor identify LA, linoleic acid Ligand MD, molecular dynamics membrane fusion MERS, Middle East respiratory syndrome modulate Molecular dynamics simulation motif mutant Mutation NTD, N-terminal domain Protein RBD, receptor binding domain RBM, receptor-binding motif receptor-binding motif Region regions removal RMB, receptor binding motif SARS-CoV-2 spike protein SARS-CoV-2, severe acute respiratory syndrome 2 SARS, Severe acute respiratory syndrome Science significantly Simulation Spike protein the SARS-CoV-2 the SARS-CoV-2 virus transmitted variant [DOI] 10.1016/j.csbj.2021.12.011 PMC 바로가기 [Article Type] Communications
First diagnosis of thrombotic thrombocytopenic purpura after SARS-CoV-2 vaccine – case reportCase Report Published on 2021-12-112022-10-28 Journal: BMC Nephrology [Category] COVID-19, SARS, [키워드] Acute kidney injury addition Admission alteration antibody Case report Corticosteroid COVID-19 vaccines described Diagnosis diagnostic differential diagnosis disintegrin dose excluded Fever Final first dose first symptom headache Hematuria Hemolysis hemolytic anemia immune initial initiated malaise male Mild Moderna motif mRNA-1273 mRNA-based nausea neurological normalized pathophysiological Patient petechial bleeding plasma exchange Platelet purpura receive receiving reported resolved SARS-CoV-2 SARS-CoV-2 vaccination SARS-CoV-2 vaccine Symptom the patient therapy thrombocyte thrombocytopenia thrombotic Thrombotic thrombocytopenic Thrombotic thrombocytopenic purpura USA vaccination Vaccine Vaccines vomiting while [DOI] 10.1186/s12882-021-02616-3 PMC 바로가기 [Article Type] Case Report