Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through ‘reverse phenotyping’단일 세포 RNA 시퀀싱은 '역 표현형'을 통해 SARS-CoV-2 반응성 T 세포의 생체 외 서명을 밝힙니다.Article Published on 2021-07-262022-09-10 Journal: Nature Communications [Category] SARS, 진단, 치료기술, [키워드] absence activated Activation acute respiratory syndrome Antigen antigenic antigenic stimulation approach cellular cellular immunity characterisation cohorts Conventional coronavirus disease Coronavirus disease 2019 correspondence COVID-19 detect Ex vivo high resolution human leukocyte antigen identify immune response in vitro in vivo independent induce Infection introduced leukocyte Patient patients phenotype Phenotypes phenotypic phenotypic effect powerful tool presence or absence provide reactive respiratory respiratory tract reveal RNA sequencing SARS-CoV-2 sequence sequences Severe acute respiratory syndrome severe disease Single Cell Single-cell RNA sequencing Stimulation T cell T cell receptor T cell response T cells T-cell receptor TCR TCRs the SARS-CoV-2 tissue tissues transcriptional viral infection virus [DOI] 10.1038/s41467-021-24730-4 PMC 바로가기 [Article Type] Article
Comparison of metabolites and variety authentication of Amomum tsao-ko and Amomum paratsao-ko using GC–MS and NIR spectroscopyArticle Published on 2021-07-262022-10-04 Journal: Scientific Reports [Category] 바이오마커, [키워드] Amomum Analysis Biochemistry Biomarker can be used China component discrepancy Gas Chromatography-Mass Spectrometry metabolite metabolites metabolomics morphology near-infrared spectroscopy NIRS partial least square PCA phenotype phenotypic Plant sciences principal component analysis tissues variety were used [DOI] 10.1038/s41598-021-94741-0 PMC 바로가기 [Article Type] Article
Emerging Approaches to Understanding Microvascular Endothelial Heterogeneity: A Roadmap for Developing Anti-Inflammatory TherapeuticsReview Published on 2021-07-212022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, [키워드] Affect alteration blood vessel bMFA cellular and molecular Developing differentially expressed endothelial barrier permeability Endothelial cell Endothelium expressed functional variation Hemostasis heterogeneity in vitro model in vivo Inflammation inflammatory disease inner layer intrinsic leukocyte leukocytes Microphysiological systems microvascular endothelial cells Morphological neutrophil recruitment organ organ damage organ-specific organs phenotypic protein kinase Cδ proteomics reactive oxygen specy regulate regulated Regulation RNA sequencing Sepsis systemic systemic inflammatory response Therapeutics transmigration understanding Variation Vascular endothelium [DOI] 10.3390/ijms22157770 PMC 바로가기 [Article Type] Review
SARS-CoV-2 Spike Mutations, L452R, T478K, E484Q and P681R, in the Second Wave of COVID-19 in Maharashtra, IndiaArticle Published on 2021-07-202022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] ACE2 binding acute respiratory syndrome Affect Against Analysis approach B.1.617.1 B.1.617.2 binding change circulating cleavage Continuous coronavirus country COVID-19 D614G Epidemiology Evolution furin cleavage site genomic help identify India indicated L452R Lineage lineages mAbs Maharashtra modeling monoclonal antibody Mutant strains Mutation neutralization P681R pandemic phenotypic RBD RBD mutation RBD mutations reported residue responsible resulting SARS-CoV-2 second second wave sequence Sequencing spike Spike protein strain Strains Structural analysis T478K the receptor-binding domain the spike protein Transmissibility Transmission variant VoC VOCs Wave whole genome whole genomes [DOI] 10.3390/microorganisms9071542 PMC 바로가기 [Article Type] Article
Multiplex SARS-CoV-2 Genotyping Reverse Transcriptase PCR for Population-Level Variant Screening and Epidemiologic Surveillance인구 수준 변이 스크리닝 및 역학 감시를 위한 다중 SARS-CoV-2 유전자형 역전사효소 PCRArticle Published on 2021-07-192022-09-11 Journal: Journal of Clinical Microbiology [Category] SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 among others approach Area B.1.1.7 B.1.351 B.1.427 B.1.429 B.1.526 clinically concerning Concordance coronavirus COVID-19 detect E484K E484K mutation E484K mutations equipment facilitate failure rate Genomic surveillance Genotype genotyping Infection L452R Laboratory large cohort multiplex Mutation mutations N501Y N501Y mutation NAAT nonsynonymous nucleic acid nucleic acid amplification nucleic acid amplification test P.2 variants pandemic PCR phenotypic Prevalence public health qPCR resource reverse transcriptase reverse transcriptase PCR RT-qPCR San Francisco San Francisco Bay SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2-positive specimen screened Screening Sequencing severe acute respiratory syndrome Coronavirus specificity specimen Spike protein subset Support Surveillance unique variant viral spike protein whole-genome sequencing [DOI] 10.1128/JCM.00859-21 PMC 바로가기 [Article Type] Article
Differing susceptibility of C57BL/6J and DBA/2J mice—parents of the murine BXD family, to severe acute respiratory syndrome coronavirus infectionLetter to the Editor Published on 2021-07-192022-11-01 Journal: Cell & Bioscience [Category] COVID-19, [키워드] accompanied acute respiratory syndrome Asymptomatic BXD family C57BL/6J caused Cohort complex conditions coronavirus coronavirus disease-2019 Coronavirus infection Coronavirus-2 COVID-19 DBA/2J develop disease manifestation Disease progression dissect evidenced Factors feasibility fraction Genetic genetic difference genetics Health Host infected individuals Infection intranasal life-threatening MA15 majority mice microbial infections Mild symptom modulate mouse-adapted SARS-CoV murine Novel coronavirus outcome pandemic parent phenotypic pulmonary viral Replication SARS-CoV SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe disease strain Support susceptibility sustained System systemically systems genetics Variation viral challenge viral clearance viral replication Virus-host interaction weight loss [DOI] 10.1186/s13578-021-00656-8 PMC 바로가기 [Article Type] Letter to the Editor
Recombinant SARS-CoV-2 genomes circulated at low levels over the first year of the pandemicResearch Article Published on 2021-07-152022-10-28 Journal: Virus Evolution [Category] COVID-19, [키워드] Adjusting Analysis approach Betacoronavirus Characteristics characterized circulating circulation clade clades Co-infection coronavirus coronavirus 2 criteria D614G de novo mutation determine E484K effort Evidence feasibility generate Genetic Genome Genotype hotspot indicated L452R Lineage lineages majority marker MONITOR Mutation N501Y pandemic parental phenotypic Phylogenetic analysis predicted Probability recombinant recombinants Recombination Region respiratory SARS-CoV-2 SARS-CoV-2 genome sequence strain subset Substitution Support the SARS-CoV-2 genome Transmissibility unique USA Variation virulence virus while [DOI] 10.1093/ve/veab059 PMC 바로가기 [Article Type] Research Article
Myeloid phenotypes in severe COVID-19 predict secondary infection and mortality: a pilot studyResearch Published on 2021-07-142022-10-28 Journal: Annals of Intensive Care [Category] COVID-19, [키워드] Admission Analysis analyzed Antigen Cell Characteristics characterized circulating COVID-19 COVID-19 patient Critical Critically ill critically ill patients diagnose dysregulation evaluate expression flow cytometry HLA-DR host response Immune cell immunological Infection intensive care leukocyte lymphocyte mHLA-DR monocyte Mortality myeloid myeloid cell myeloid-derived suppressor cells Occurrence Patient patients died patients with COVID-19 phenotype phenotypic Phenotyping pilot study predict Result Secondary infection Sepsis Sepsis-induced immunodepression severe coronavirus disease severe COVID-19 significantly subpopulation supplementary material was performed [DOI] 10.1186/s13613-021-00896-4 PMC 바로가기 [Article Type] Research
Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium인간 재구성 기관지 상피에서 혈통 B.1.1.7의 SARS-CoV-2 20I/501Y.V1 변이체의 복제 적합성Observation Published on 2021-07-062022-09-10 Journal: mBio [Category] MERS, SARS, 변종, [키워드] 20I/501Y.V1 B.1.1.7 both virus both viruses bronchial Bronchial epithelium cellular Characteristics circulating competition contribute coronavirus CoV D614G epithelium European Evidence Ex vivo experiment fitness genetic diversity Human in vitro Lineage Lineage B.1.1.7 New coronavirus no difference phenotypic Population regions replaced replicate Replication replication kinetics replicative fitness respiratory Result SARS-CoV-2 strain variant viruses [DOI] 10.1128/mBio.00850-21 PMC 바로가기 [Article Type] Observation
Low SARS‐CoV‐2 seroprevalence in a cohort of Brazilian sickle cell disease patients: Possible effects of emphasis on social isolation for a population initially considered to be at very high riskShort Report Published on 2021-06-172022-10-31 Journal: Ejhaem [Category] COVID-19, [키워드] Cell Clinical severity Cohort Contagion contributed COVID‐19 determinant disease Effect Factor General population high risk higher risk Infection Older age Patient phenotypic Prevalence remains uncertain SARS‐CoV‐2 SCD serologic social distancing social isolation was performed [DOI] 10.1002/jha2.254 PMC 바로가기 [Article Type] Short Report