Receptor Affinity-Based Purification of PfEMP1 Proteinsarticle Published on 2022-12-312024-09-01 Journal: Methods in molecular biology (Clifton, N.J.) [Category] 말라리아, [키워드] Affinity purification CIDR DBL ELISA Ligand–receptor interaction malaria PfEMP1 [Article Type] article
Affinity Purification of PfEMP1-Specific Antibodies from Human Bloodarticle Published on 2022-12-312024-09-01 Journal: Methods in molecular biology (Clifton, N.J.) [Category] 말라리아, [키워드] Affinity purification antibodies ELISA malaria natural acquired immunity PfEMP1 [Article Type] article
RANBP2 and USP9x regulate nuclear import of adenovirus minor coat protein IIIaRANBP2 및 USP9x는 아데노바이러스 마이너 코트 단백질 IIIa의 핵 수입을 조절합니다Article Published on 2022-06-162022-09-11 Journal: PLoS Pathogens [Category] 유전자 메커니즘, [키워드] accumulation acute respiratory illness acute respiratory illnesses Adenovirus Affinity purification Amino acid amino acids Analysis antiviral therapeutics antiviral therapy bind binding blockade C-terminus Cell cell nuclei cells cellular competition conjunctivitis counteract COVID-19 demonstrated deregulated disparate expression Host implication indicated infected cells Infection interact Interaction interactions mass spectrometry maturation mechanism Microscopy nuclear nuclei nucleus outbreak parasites protease Protein Proteins Ranbp2 reduced regulate respiratory pathogen retention subsequent surface plasmon surface plasmon resonance transmission electron microscopy Transport USP9x Vaccine viral genome replication Viral particles Viral protein virion virion assembly virus virus-infected cell virus-infected cells wild type [DOI] 10.1371/journal.ppat.1010588 PMC 바로가기 [Article Type] Article
SARS-CoV-2 proteome microarray for COVID-19 patient sera profilingCOVID-19 환자 혈청 프로파일링을 위한 SARS-CoV-2 프로테옴 마이크로어레이Article Published on 2022-02-282022-09-12 Journal: STAR protocols [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] acute respiratory syndrome Affinity purification Antibody Response Biotechnology Biotechnology and bioengineering build Cell clinical clinical protocol Complete coronavirus COVID-19 patient E. coli eukaryotic expression facilitate Health Sciences help IgG and IgM Immunity immunology include Microarray Protein Protein Biochemistry Protein expression and purification proteome proteomics protocol SARS-CoV-2 SARS-CoV-2 proteome sera were expressed [DOI] 10.1016/j.xpro.2022.101238 PMC 바로가기 [Article Type] Article
The interactions of ZDHHC5/GOLGA7 with SARS-CoV-2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entrySARS-COV-2 스파이크 (S)와 ZDHHC5/GOLGA7의 상호 작용 및 S 단백질의 세포 내 위치, 팔미 토일 화 및 슈도 바이러스 진입에 미치는 영향Research Published on 2021-12-272022-09-10 Journal: Virology Journal [Category] SARS, 치료기술, [키워드] A549 acute respiratory syndrome acute respiratory syndrome coronavirus Affect affected Affinity purification analyzed Antiviral APT2 assays Biotin Cas9 Cell cell line cell lines cell types cells co-immunoprecipitation co-IP Colocalization complex coronavirus CRISPR CRISPR-Cas9 cysteine cysteine residue determine domain Effect Efficiency endodomain enzymatic activity enzyme fluorescence microscopy GOLGA7 had no HEK293T HEK293T cell HEK293T cells Hela HeLa cells host protein host proteins independent independent of interact Interaction knowledge mass spectrometry no significant effect palmitoylation Protein provide pseudovirus entry reason Renilla Renilla luciferase respiratory Result S protein S proteins SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 S protein SARS-CoV-2 spike severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significant effect significantly Spike protein subcellular localization the cell the S protein virus entry virus infection Virus-host interaction ZDHHC5 ZDHHC5/GOLGA7 [DOI] 10.1186/s12985-021-01722-w PMC 바로가기 [Article Type] Research
SARS‐CoV‐2–host proteome interactions for antiviral drug discovery항바이러스 약물 발견을 위한 SARS-CoV-2-호스트 프로테옴 상호작용Article Published on 2021-10-282022-09-10 Journal: Molecular Systems Biology [Category] MERS, SARS, 신약개발, 유전자 메커니즘, 치료제, [키워드] addition Affinity purification analyses Analysis antiviral activity antiviral drug Antiviral effect antiviral effects applied approach biological significance candidate drug caused cofactor cofactors complementary Compound COVID‐19 criterion Critical develop downstream drug Drug discovery effective therapy Host host cell host cell proteases host protein host proteins hub protein identify in viral inhibitory effect Interaction labeling list mass spectrometry methotrexate Microbiology, Virology & Host Pathogen Interaction molecular ORF Pharmacology & Drug Discovery protein targets protein–protein interactions proteome proteomics provided receptors Replication resource SARS‐CoV‐2 SARS‐CoV‐2 suggested understanding Viral viral pathogenesis viral replication Virtual screening virus–host interactions [DOI] 10.15252/msb.202110396 PMC 바로가기 [Article Type] Article
Interactome Analysis of the Nucleocapsid Protein of SARS-CoV-2 VirusArticle Published on 2021-09-082022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] Affinity purification Analysis antiviral strategy binding biogenesis calu-3 cells caused cellular complex damaged EIF2AK2 functions G3BP1 global pandemic HEK293T human cell human cells Infection interaction partner interactome MOV10 multifunctional N protein nucleocapsid protein Pathogenesis Protein public health responses ribonucleocapsid ribonucleoprotein ribosome biogenesis RNA SARS-CoV-2 SARS-COV-2 infection small subunit the N protein the SARS-CoV-2 Transcription translation TRIM25 viral genome replication viral–host interactome virus ZC3HAV1 ZCCHC3 [DOI] 10.3390/pathogens10091155 PMC 바로가기 [Article Type] Article
Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesisSARS-CoV-2와 인간 코로나바이러스의 상호작용은 잠재적으로 병인에 영향을 미치는 숙주 요인을 나타냅니다Article Published on 2021-09-012022-09-11 Journal: The EMBO Journal [Category] SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affecting Affinity purification BioID2 cellular response Cellular responses comparison conducted coronavirus COVID-19 disease disease pathology drug target encoded viral proteins explain Factor Host host cell host cells host-virus protein-protein interaction human coronavirus human coronaviruses human protein human proteins Interaction interactome life cycle nsp1 Pathogenesis pathway Protein protein-protein interaction Proteins provide SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 viral severe acute respiratory syndrome Coronavirus SFB-TAP survival These data these viruses treating Treatment treatment of COVID-19 valuable resource Viral proteins virus [DOI] 10.15252/embj.2021107776 PMC 바로가기 [Article Type] Article
Mapping the SARS-CoV-2–Host Protein–Protein Interactome by Affinity Purification Mass Spectrometry and Proximity-Dependent Biotin Labeling: A Rational and Straightforward Route to Discover Host-Directed Anti-SARS-CoV-2 TherapeuticsSARS-CoV-2–숙주 단백질–단백질 상호작용체 매핑(Affinity Purification Mass Spectrometry 및 Proximity-Dependent Biotin Labeling: Host-Directed Anti-SARS-CoV-2 치료제 발견을 위한 합리적이고 간단한 경로)Review Published on 2021-01-072022-09-10 Journal: International Journal of Molecular Sciences [Category] 신약개발, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 advent affinity Affinity purification affinity-purification mass spectrometry Antiviral approach approved drug cellular cellular processe cellular processes coronavirus coronavirus disease COVID-19 Drug discovery Drug repurposing effective emerging experiment Follow-up Generation help high resolution Host host cell host cells host protein identify Interaction interactome life cycle mapping mass mass spectrometry mirage offer outbreak PPI protein interactions protein–protein interactions protein–protein interaction proteomics proximity-dependent biotin labeling (BioID) reduce regulated respiratory route SARS-CoV-2 severe acute respiratory syndrome Coronavirus Spectrometry target targets therapeutic option therapeutic targets Therapeutics Viral viral life cycle virus virus entry virus–host interaction virus–host interactions [DOI] 10.3390/ijms22020532 PMC 바로가기 [Article Type] Review
SARS-Cov-2 Interactome with Human Ghost Proteome: A Neglected World Encompassing a Wealth of Biological DataCommunication Published on 2020-12-192022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] addition Affinity purification alternative ORF alternative protein anti-viral response Biological cellular co-immunoprecipitation co-IP COVID-19 outbreak database DMPK Drug repurposing eukaryotic Evidence function functional Ghost ghost protein host cell Human identify initial innate immune response Interaction interactions interactome interactomics involved metabolism missing mRNA ncRNAs Neglected non-coding RNA OpenProt ORF Perspective pharmacokinetic physiological predicted Protein proteome proteomics responsible SARS-CoV-2 target the SARS-CoV-2 the SARS-CoV-2 virus Therapeutic strategies therapeutic targets Therapies thought translated translation Viral protein Viral proteins [DOI] 10.3390/microorganisms8122036 PMC 바로가기 [Article Type] Communication