More tools for our toolkit: The application of HEL-299 cells and dsRNA-nanoparticles to study human coronaviruses in vitroArticle Published on 2022-11-012022-11-15 Journal: Virus research [Category] SARS, 치료법, 치료제, [키워드] ACE2 receptor Analysis Antiviral antiviral genes antiviral response antiviral therapy can be used Cell cell line cells Combination competent Complete coronavirus Coronavirus replication CXCL10 cytidylic acid double-stranded expand fibroblast Gene Expression HCoV-229E HCoV-NL63 help Human human coronavirus human coronaviruses human lung IFN IFNs IFNβ in vitro increase in inducer Infection inhibit innate immune interferons ISG15 limit MERS-CoV Nanoparticles Pathogens Poly I:C. post infection PROTECT provided required SARS-CoV-2 SARS-CoV-2 pandemic stimulate translated Treatment Type I IFN type I interferon virus production were infected while [DOI] 10.1016/j.virusres.2022.198925 PMC 바로가기
An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19N 단백질 결합 앱타머-상보성 올리고뉴클레오티드를 COVID-19의 초고감도 검출을 위한 프로브로 사용하는 전기화학적 앱타센서Article Published on 2022-10-012022-09-11 Journal: Biosensors & bioelectronics [Category] 치료기술, 치료제, [키워드] affected applied aptamer binding blood sample Blood samples changes in complementary Complementary DNA Concentration conformational COVID-19 COVID-19 epidemic COVID-19 in patients DNA double-stranded Early detection effective Electrochemical aptasensor gold hybridization Incubation linear relationship log logarithm N protein N protein detection probe R 2 Rapid Rapid COVID-19 diagnosis Regenerable aptamer sensor. reported sensor solution target the N protein throat swab throat swabs vaccination volunteers was measured [DOI] 10.1016/j.bios.2022.114436 PMC 바로가기 [Article Type] Article
Pin-Pointing the Key Hubs in the IFN-γ Pathway Responding to SARS-CoV-2 InfectionArticle Published on 2022-09-302022-11-16 Journal: Viruses [Category] SARS, 치료법, [키워드] A549 Analysis biopsy boost case-control study CBL confirmation COVID-19 COVID-19 patients displaying double-stranded expression gene expression profile highlighting host response Hub human cell line IFN-stimulated gene IFN-γ Immunotherapy in vitro infected with SARS-CoV-2 Infection ISG ISG15 ISGs ISGs. JAK2 liver lung lung cell MAP2K6 mice murine model MX1 MX2 OAS1 pathway patients positive correlation positive patients Responding RUNX3 SARS-CoV-2 SARS-COV-2 infection Stat1 target the patient tracheal treated upregulation Viral load was performed [DOI] 10.3390/v14102180 PMC 바로가기
New challenges in human monkeypox outside Africa: A review and case report from ItalyReview article Published on 2022-09-012022-10-05 Journal: Travel medicine and infectious disease [Category] 비임상, 원숭이두창, [키워드] Anal Case report caused confirmed case Contact diagnosed Diagnosis disease DNA virus double-stranded Endemic HIV Human human-to-human transmission Italian Italy men Monkeypox New orthopoxvirus outbreak PCR Portugal positive Poxviridae responsible rodent Sample Sex Singapore skin lesions the United State United Kingdom USA Viral load virus virus infection zoonotic disease [DOI] 10.1016/j.tmaid.2022.102386 [Article Type] Review article
Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3 Research Published on 2022-08-302022-10-05 Journal: Nature Communications [Category] 변종, 유전자 메커니즘, 진단, [키워드] atomic binding cleavage Complete complex CRISPR Degradation degrade detect DNA DNA degradation double-stranded dsDNA dynamic elicit Escherichia coli exhibit Genome in vitro mechanism Microscopy multi non-specific NTS nucleic acid PAM Partial priming Protein R-loop recruited Release sequence single-stranded DNA spacer ssDNA Type type I [DOI] 10.1038/s41467-022-32618-0 [Article Type] Research
Flipped over U: structural basis for dsRNA cleavage by the SARS-CoV-2 endoribonuclease뒤집힌 U: SARS-CoV-2 엔도리보뉴클레아제에 의한 dsRNA 절단의 구조적 기초Article Published on 2022-08-122022-09-11 Journal: Nucleic Acids Research [Category] SARS, 유전자 메커니즘, 치료법, [키워드] activate Activation active site cleavage cleave coronavirus Coronavirus replication Critical cryo-EM structure cryo-EM structures double-stranded dsRNA endoribonuclease evade form generate Host immune MDA5 mechanism Nsp15 Pattern recognition pattern recognition receptor platform processing recognize replication-transcription complex RNA SARS-CoV-2 SARS-CoV-2 Nsp15 single-stranded site-directed mutagenesis structures substrates the SARS-CoV-2 uridine Vesicle viral replication [DOI] 10.1093/nar/gkac589 PMC 바로가기 [Article Type] Article
Monkeypox: An emerging global threat during the COVID-19 pandemicReview article Published on 2022-08-012022-10-05 Journal: Journal of microbiology, immunology, and infection [Category] 원숭이두창, 치료기술, 치료제, [키워드] activity affected African antiviral therapy Atypical body fluids Care Case isolation clade Contact Contact tracing COVID-19 COVID-19 pandemic develop Diagnosis Diseases DNA virus double-stranded effective strategy Features genetic data Health health system human-to-human transmission include laboratory-confirmed lesion mitigate Monkeypox nucleic acid amplification outbreak Outbreaks patients polymerase chain public health Rash reported skin lesion Spread symptom onset Symptoms Transmission vaccination virus virus infection West [DOI] 10.1016/j.jmii.2022.07.004 [Article Type] Review article
Discovery Proteomics Analysis Determines That Driver Oncogenes Suppress Antiviral Defense Pathways Through Reduction in Interferon-β Autocrine StimulationDiscovery Proteomics 분석을 통해 드라이버 종양유전자가 인터페론-β 자가분비 자극 감소를 통해 항바이러스 방어 경로를 억제한다는 사실이 확인되었습니다.Article Published on 2022-07-012022-09-11 Journal: Molecular & Cellular Proteomics : MCP [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, [키워드] activated AKT Antiviral approach autocrine BRAF c-MYC Cancer cancer cell line cancer cell lines cancers Cell cell line cellular DDX58 develop double-stranded double-stranded RNA downregulation driven by driving dsRNA EGFR expression functional hallmark HER2 immune evasion Infection interferon KRAS lower expression MEK molecular MYC OAS oncogene oncogenes. oncogenic osteosarcoma Pancreatic cancer phenotypic profiles Protein Proteins proteome provide reduced regulated response RIG-I RNA virus SARS-CoV-2 shown Stimulation susceptibility susceptibility to infection therapeutic treated type 1 interferon [DOI] 10.1016/j.mcpro.2022.100247 PMC 바로가기 [Article Type] Article
Development and validation of a PCR-free nucleic acid testing method for RNA viruses based on linear molecular beacon probes선형 분자 비콘 프로브를 기반으로 하는 RNA 바이러스에 대한 PCR-free 핵산 검사 방법 개발 및 검증Article Published on 2022-06-112022-09-11 Journal: Journal of Nanobiotechnology [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification analyses applicability bind classical complementary Concentration confocal microscopy coronavirus CSFV Detection limit development diagnostic distribution domain double-stranded FAM Fever fluorophore host cells human diseases linear molecular Molecular beacon probes N genes nucleic acid nucleic acid extraction nucleic acid testing nucleic acids Pandemics PCR-free platform POC point-of-care probe qRT-PCR Quantitative RNA RNA virus RNA viruses SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 N gene severe acute respiratory syndrome Coronavirus supported tissues viral nucleic acid viral nucleic acids virus were used [DOI] 10.1186/s12951-022-01470-1 PMC 바로가기 [Article Type] Article
Insights into the specificity for the interaction of the promiscuous SARS-CoV-2 nucleocapsid protein N-terminal domain with deoxyribonucleic acids난잡한 SARS-CoV-2 뉴클레오캡시드 단백질 N-말단 도메인과 데옥시리보핵산의 상호작용에 대한 특이성에 대한 통찰력Article Published on 2022-04-012022-09-11 Journal: International journal of biological macromolecules [Category] COVID19(2023년), SARS, 치료법, [키워드] activity approximation binding Binding specificity cleft dissect DNA DNA binding DNA/RNA binding protein double-stranded feature inhibitors insight Interaction Local motif multifunctional N-terminal domain non-specific nucleic acid nucleocapsid parameter preference Protein RNA RNA binding protein SARS-CoV-2 nucleocapsid SARS-CoV-2 nucleocapsid protein sequence specificity starting point suggested the N protein therapeutic target thermodynamic thymidine Transcription transcriptional regulatory sequence transcriptional regulatory sequences TRS virus infectivity β-sheet [DOI] 10.1016/j.ijbiomac.2022.01.121 PMC 바로가기 [Article Type] Article