Improved sensitivity and automation of a multi-step upconversion lateral flow immunoassay using a 3D-printed actuation mechanismResearch Paper Published on 2024-01-272024-09-05 Journal: Analytical and bioanalytical chemistry [Category] update2024, [키워드] bioassays biomedical analysis Luminescence Nanoparticles polymers Rare earth elements [DOI] 10.1007/s00216-024-05156-5 PMC 바로가기 [Article Type] Research Paper
A pseudovirus-based platform to measure neutralizing antibodies in Mexico using SARS-CoV-2 as proof-of-conceptArticle Published on 2022-10-262022-11-15 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 진단, 치료제, [키워드] Accuracy antiviral immune response applied BSL-2 BSL-3 BSL-3 facilities evaluate evidenced functional in vitro Infection information Latin lentiviral particles Live virus Luminescence methodology NAb NAbs neutralizing antibody non-replicative Particle pathogen performed platform Protein pseudotyped lentiviral particles pseudovirus reporter resource Safe SARS-CoV-2 selected sensitivity serum samples specificity target cell transduced viral pathogen [DOI] 10.1038/s41598-022-22921-7 PMC 바로가기
A Cellular Assay for Spike/ACE2 Fusion: Quantification of Fusion-Inhibitory Antibodies after COVID-19 and VaccinationArticle Published on 2022-09-252022-11-15 Journal: Viruses [Category] SARS, 진단, 치료기술, [키워드] absence ACE2 antibody biosafety Cell Cell fusion co-cultured complementary Complete correlated COVID-19 detect dual-split luciferase ELISA first symptom individual Infection inhibit Interaction LIGHT Luminescence neutralizing antibody occurred Patient Proteases reduced reduction SARS-CoV-2 serology serum spike Standardization transduced vaccinated individual viral entry [DOI] 10.3390/v14102118 PMC 바로가기
Ultraviolet-coupled advanced oxidation processes for anti-COVID-19 drugs treatment: Degradation mechanisms, transformation products and toxicity evolutionResearch article Published on 2022-09-012022-10-05 Journal: Chemosphere [Category] 치료기술, 치료법, 치료제, [키워드] Analysis anti-COVID-19 drugs Appropriate Bacterial Combined COVID-19 Degradation degradation mechanism Dexamethasone drug Evolution functional H2O2 HCQ Hydroxychloroquine Intermediates analysis investigated Luminescence Luminescent bacterial test mechanisms parameter pathway Pneumonia positive predicted RDV Remdesivir risk significantly higher Toxicity Treatment UV/AOPs viruses [DOI] 10.1016/j.chemosphere.2022.134968 [Article Type] Research article
Luminescent Assay for the Screening of SARS-CoV-2 M Pro InhibitorsSARS-CoV-2 M Pro 억제제의 스크리닝을 위한 발광 분석Article Published on 2022-08-032022-09-11 Journal: Chembiochem [Category] COVID19(2023년), SARS, 치료제, [키워드] antiviral drug can be used cleavage site Enzymes exhibit imaging agents in viral Infection inhibitor inhibitors Luminescence luminescence. M pro outbreak peptide Pro probe protease Replication reported SARS-CoV-2 Screening viral replication [DOI] 10.1002/cbic.202200190 PMC 바로가기 [Article Type] Article
Intranasal inoculation of an MVA-based vaccine induces IgA and protects the respiratory tract of hACE2 mice from SARS-CoV-2 infectionMVA 기반 백신의 비강내 접종은 IgA를 유도하고 SARS-CoV-2 감염으로부터 hACE2 마우스의 호흡기를 보호합니다Article Published on 2022-06-142022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] acute respiratory syndrome administration Antigen Beta Blood CD3 CD8 coronavirus coronavirus 2 coronavirus vaccine COVID-19 COVID-19 pandemic current effective expressing firefly luciferase hACE2 IFN-γ IgA IgG immune responses Immunity immunized Immunoglobulin immunoglobulin A Immunoglobulin G induce Infection Infectious virus intramuscular intramuscular vaccination intramuscularly intranasal intranasally K18-hACE2 less luciferase Luminescence lung Lungs mice modified vaccinia virus morbidity mucosal mucosal immunity nasal turbinate nasal turbinates neutralized Neutralizing antibodies neutralizing antibody omicron Prevent prevented priming PROTECT protective immune response pseudovirus pseudoviruses raise remained respiratory respiratory immunity respiratory tract S protein S proteins SARS-CoV-2 SARS-COV-2 infection serum severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severity T cells the S protein Transmission upper respiratory tract vaccination Vaccine vaccinia virus variants Viral while Wuhan [DOI] 10.1073/pnas.2202069119 PMC 바로가기 [Article Type] Article
Hybridization chain reaction circuit-based electrochemiluminescent biosensor for SARS-cov-2 RdRp gene assaySARS-cov-2 RdRp 유전자 분석을 위한 혼성화 연쇄 반응 회로 기반 전기화학발광 바이오센서Article Published on 2022-04-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] activated amplification applied Au-g-C(3)N(4) Base pairing Chain Reaction complementary complex decrease detect Detection limit DNA ECL gradually increased HCR hybridization Luminescence nucleic acids probe quantified RdRP RdRp gene SARS-CoV-2 nucleic acid SARS-CoV-2 RdRp SARS-CoV-2 RdRp gene signal target gene the SARS-CoV-2 this system Y-DNA. [DOI] 10.1016/j.talanta.2022.123207 PMC 바로가기 [Article Type] Article
Alternative Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies중증 급성 호흡기 증후군 코로나바이러스 2 항체를 검출하는 대체 방법Review Published on 2022-03-012022-09-11 Journal: Clinics in laboratory medicine [Category] MERS, SARS, 진단, 치료기술, [키워드] approaches coronavirus 2 COVID-19 pandemic diagnostic Electrochemical detection ELISA enzyme-linked immunosorbent enzyme-linked immunosorbent assay health information immunoassay immunoassays lateral flow Luminescence measurement MOST Neutralization assay Neutralizing antibodies neutralizing antibody pros respiratory SARS-CoV-2 serologic assay serologic assays serology Spread virus [DOI] 10.1016/j.cll.2021.10.007 PMC 바로가기 [Article Type] Review
Engineered RNA biosensors enable ultrasensitive SARS-CoV-2 detection in a simple color and luminescence assay엔지니어링된 RNA 바이오센서는 간단한 색상 및 발광 분석에서 초고감도 SARS-CoV-2 검출을 가능하게 합니다.Research Article Published on 2021-09-302022-09-10 Journal: Life Science Alliance [Category] SARS, 변종, 치료기술, [키워드] Activation amplification conformational COVID-19 COVID-19 pandemic detect diagnostic diagnostics example fragment fragment of isothermal amplification lab LacZ lantern leads Luminescence Patient PHANTOM Protein reporter resource RNA SARS-CoV-2 detection SARS-CoV-2 genome SARS-CoV-2 RNA sensor Spectrophotometry the SARS-CoV-2 genome translation variant variants Viral viral evolution Viral RNA virus [DOI] 10.26508/lsa.202101213 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 RdRp Inhibitors Selected from a Cell-Based SARS-CoV-2 RdRp Activity Assay SystemArticle Published on 2021-08-112022-10-28 Journal: Biomedicines [Category] COVID-19, [키워드] activity acute respiratory syndrome adefovir dipivoxil calculated candidate caused Cell Cepharanthine conserved coronavirus Coronavirus infection Coronavirus infections COVID-19 COVID-19 pandemic double-stranded RNA drugs effective emtricitabine entecavir hydrate expression immunofluorescence inhibiting inhibitors Luminescence lycorine MERS-CoV moroxydine nucleotide Plasmid platform Prophylactic provide RdRP reliability Remdesivir replicated reported reporter plasmid reproducibility rifampin RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 RdRp SARS-CoV-2 RdRp inhibitor screened staining System telbivudine therapeutic therapeutic drug Therapeutics this system viral RNA genome Z-factor [DOI] 10.3390/biomedicines9080996 PMC 바로가기 [Article Type] Article