Evaluation of Host Protein Biomarkers by ELISA From Whole Lysed Peripheral Blood for Development of Diagnostic Tests for Active Tuberculosis활동성 결핵 진단 테스트 개발을 위한 전 용해 말초 혈액에서 ELISA에 의한 숙주 단백질 바이오마커 평가Article Published on 2022-05-202022-09-11 Journal: Frontiers in Immunology [Category] 바이오마커, [키워드] analyses assay associative barrier Biomarker Biomarkers blood sample CALCOCO2 capture cause of death caused CD274 CD52 collected combinations complex conducted confirmatory tests consequence Control correlation country COVID-19 pandemic death defined development diagnosing Diagnosis diagnostic diagnostic test differentially expressed disease Diversity downregulated ELISA ENhance eradication evaluate expression GBP1 group groups Health Health crisis IFIT3 IFITM3 improvement Improvements incidence India individuals infected individuals Infection Infectious disease information investigated Key priorities key priority Laboratory Lateral flow device lysed negative controls nine Patient peripheral profile Protein protein expression Protein target protein targets qPCR Rapid reducing required ROC SAMD9L significantly SNX10 Stratification Test the WHO Tuberculosis validation study WHO World Health Organisation [DOI] 10.3389/fimmu.2022.854327 PMC 바로가기 [Article Type] Article
Inactivation and Recovery of High Quality RNA From Positive SARS-CoV-2 Rapid Antigen Tests Suitable for Whole Virus Genome Sequencing전체 바이러스 게놈 시퀀싱에 적합한 양성 SARS-CoV-2 신속 항원 테스트에서 고품질 RNA의 비활성화 및 회수Article Published on 2022-05-032022-09-11 Journal: Frontiers in Public Health [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료법, [키워드] aligned Antigen antigen testing applied B.1.1.7 can be used circulating variant circulating variants Community Concentration conserved coronavirus 2 demonstrated detect diagnostic diagnostic protocol diagnostic test diagnostic tests each time-point epidemiological Epidemiology Genome genomic sequence identify imperative inactivated Infection lateral flow Lateral flow device Lineage Lineage B.1.1.7 lysed lysis management Omicron variant outbreak positive positive result protocol public health qPCR Quality Rapid Rapid antigen diagnostic test replicating virus respiratory risk Risk management RNA RNA extraction RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 variant SARS-CoV-2 viral sequence Sequencing Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spread stability storage strain Suspension Template Test tested utility Variation viral nucleocapsid protein viral particle Viral particles Viral RNA virus virus genome was used were used whole virus genome sequencing. [DOI] 10.3389/fpubh.2022.863862 PMC 바로가기 [Article Type] Article
Peptide-based simple detection of SARS-CoV-2 with electrochemical readout전기화학적 판독으로 SARS-CoV-2의 펩티드 기반 단순 검출Article Published on 2022-05-012022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome coronavirus antigens approaches caused complex coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 detect followed by gold impedance Impedimetric biosensor infected patient infected patients Infectious disease Interaction limit of detection lysed nanomaterials outbreak pandemic path peptide platform Protein reported reproducibility respiratory SARS-CoV-2 SARS-CoV-2 particles SARS-CoV-2 spike protein Screen sensitivity Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 simplicity Spectroscopy spike Spike protein synthetic synthetic peptide tested the Spike virus [DOI] 10.1016/j.aca.2022.339739 PMC 바로가기 [Article Type] Article
Centrifugal Microfluidic Method for Enrichment and Enzymatic Extraction of Severe Acute Respiratory Syndrome Coronavirus 2 RNA중증 급성 호흡기 증후군 코로나바이러스 2 RNA의 농축 및 효소 추출을 위한 원심 미세 유체 방법Article Published on 2022-02-222022-09-11 Journal: Analytical Chemistry [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification analyst analyzed applicability buffer capsid Centrifugal Chain Reaction comparable complex controls coronavirus coronavirus 2 demonstrated development Diagnosis diagnostics downstream effective ENhance enrichment exposure to extraction imperative in viral inhibit Intervention Isolation lysed nasopharyngeal Nasopharyngeal swab nucleic acid nucleic acids Particle pathogen detection pathogenic Pathogens platform polymerase chain polymerase chain reaction positive purification real-time reverse transcriptase real-time RT-PCR removal respiratory reverse transcriptase Reverse transcriptase polymerase chain reaction RNA RNA extraction robust RT-PCR Saliva Sample SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA SARS-CoV-2 virion severe acute respiratory syndrome Coronavirus Surveillance Transmission Transport media Treatment tube Viral RNA viral transport media virus [DOI] 10.1021/acs.analchem.1c05215 PMC 바로가기 [Article Type] Article
A rapid real-time polymerase chain reaction-based live virus microneutralization assay for detection of neutralizing antibodies against SARS-CoV-2 in blood/serumResearch Article Published on 2021-12-102022-10-28 Journal: PLoS ONE [Category] COVID-19, MERS, SARS, [키워드] 24 hours antibody antiviral activity blood/serum calculate can be used conducted COVID-19 case cross-reactive Culture Cytopathic effect Dilution dilutions dose evaluate IgG titer individual individuals infected cell infective limiting dilution Live virus lysed microneutralization nasopharyngeal specimen neutralization Neutralizing Neutralizing antibodies neutralizing antibody Neutralizing assay PCR percentage polymerase chain positive positively correlated RBD recovering from COVID-19 responses Result RNA RT-PCR SARS-CoV-2 SARS-CoV-2 infected individuals SARS-CoV-2 microneutralization assay SARS-CoV-2 strain sera serum serum antibody serum samples shown spike structural proteins TCID50 tested the Spike tissue Vero cells viral neutralization virus-neutralizing was used were used [DOI] 10.1371/journal.pone.0259551 PMC 바로가기 [Article Type] Research Article
Computational Method‐Based Optimization of Carbon Nanotube Thin‐Film Immunosensor for Rapid Detection of SARS‐CoV‐2 VirusResearch Article Published on 2021-11-162022-10-28 Journal: Small Science [Category] COVID-19, [키워드] amplification analyzed applicability biosensors buffer carbon carbon nanotubes CNT complex correlated correlation COVID‐19 detection diagnosing Diagnosis diagnostic Diseases early stage lysed machine learning molecular nucleocapsid protein optimization Patient POC point‐of‐care Rapid SARS-CoV-2 SARS‐CoV‐2 SARS‐CoV‐2 virus sensitivity solution shearing Spread systematic analysis virus [DOI] 10.1002/smsc.202100111 PMC 바로가기 [Article Type] Research Article
Evaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings실험실 환경에서 SARS-CoV-2의 안정성 및 비활성화 방법 평가Original Investigation Published on 2021-07-012022-09-12 Journal: Medical Microbiology and Immunology [Category] MERS, SARS, 진단, [키워드] acetone acute respiratory disease addition Analysis analyzed Bio safety biosafety level Biosafety Level 3 BSL-3 Caco-2 causative agent Cell culture Corona virus COVID-19 Crystal violet Culture culture media denaturation dose effective Ethanol evaluated guanidine handling heat inactivation inactivation infected cell infected cells Infection Laboratory level 3 lysate lysates lysed lysis buffer media methanol Microscopy molecular Molecular biology Novel coronavirus novel coronavirus SARS-CoV-2 PFA Proteins removal required Research RT-qPCR SARS-CoV-2 Spread stability staining subsequent supernatant supplementary material susceptible TCID tested tissue tissue culture Vero Cell Vero cells viral sample virus [DOI] 10.1007/s00430-021-00716-3 PMC 바로가기 [Article Type] Original Investigation
Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccineArticle Published on 2021-06-162022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] Activation Analysis Biomarker Cell dendritic cell differentially expressed domains eight facilitated Gene regulation in immune cells homology immune responses in vivo inactivated Inactivated vaccine increased expression Infectious Bronchitis Virus Innate immune cells lipid metabolism lysed maturation mRNA mRNA expression mRNA translation newcastle disease virus PLIN2 positive control Potential biomarker Protein protein level Proteins proteomic Proteomic analysis provide PSMB1 significantly stimulated Stimulation subject the vaccine upregulated Vaccine vaccine-induced immune responses Vaccines were assessed [DOI] 10.1038/s41598-021-89810-3 PMC 바로가기 [Article Type] Article
Persistence of SARS-CoV-2 in the first trimester placenta leading to transplacental transmission and fetal demise from an asymptomatic mother임신 초기 태반에서 SARS-CoV-2가 지속되어 무증상 산모로부터 태반 전파 및 태아 사망으로 이어짐Case Reports Published on 2021-03-182022-09-11 Journal: Human reproduction (Oxford, England) [Category] SARS, 치료기술, [키워드] abortion absence acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amniotic Amniotic fluid Asymptomatic caused Cell Clinical course congenital coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 decidua demise early pregnancy edema Evidence evidence of fetal fetal membrane FIBRIN first trimester gestation hydrops fetalis in some indicated Infection Inflammation leukocyte leukocyte infiltration lysed membrane mother Placenta placental placental barrier Placental infection pleural effusion positive Pregnancy Pregnant woman Protein respiratory tract S proteins S1 and S2 SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 tested the S protein the Spike throat swab tissue tissues Transmission transmission of SARS-CoV-2 Ultrasonography vertical transmission villi Viral RNA virus [DOI] 10.1093/humrep/deaa367 PMC 바로가기 [Article Type] Case Reports
A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater SamplesFull Paper Published on 2021-02-222022-10-31 Journal: Global Challenges [Category] COVID-19, [키워드] biohazard clinical Community contagious COVID‐19 Diagnosis equipment evaluated extraction inactivated infectious sample Influenza influenza viruses Isolation Laboratory lysed Magnetic nanoparticles membrane nanoparticle nucleic acid Nucleic acid purification pathogen pathogen detection Pathogens Patient reagent reagents reducing respiratory syncytial virus risk RNA RNA extraction Safe Sample SARS‐CoV‐2 SARS‐CoV‐2 shown Surveillance Trizol Viral RNA wastewater wastewater surveillance widespread [DOI] 10.1002/gch2.202000068 PMC 바로가기 [Article Type] Full Paper