Human Nasal Organoids Model SARS-CoV-2 Upper Respiratory Infection and Recapitulate the Differential Infectivity of Emerging VariantsArticle Published on 2022-08-302022-11-16 Journal: mBio [Category] SARS, 변종, [키워드] acidic pH airway Airway organoids Cell cellular ciliary ciliary damage ciliated cell Culture Delta Efficiency generate higher infectivity Human imperative in vitro model Infection Infectivity Interaction maturation Model monolayer morphologically nasal nasal epithelial cell nasal epithelium nasal organoids nasopharyngeal omicron Organoid Primary infection protocol remained replicative fitness respiratory respiratory viruses robust SARS-CoV-2 tight junction tight junctions Transmissibility Transmission upper upper respiratory infection upper respiratory tract variant variants viral pathogenesis virus spread [DOI] 10.1128/mbio.01944-22 PMC 바로가기
Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cellsOmicron이 아닌 조상 SARS-CoV-2는 일차 소아 비강 상피 세포에서 덜 효율적으로 복제합니다Article Published on 2022-08-012022-09-11 Journal: PLoS Biology [Category] Coronavirus, SARS, 변종, [키워드] acute respiratory syndrome Adults Air-liquid interface antiviral response Biology biology of SARS-CoV-2 changes in children coronavirus 2 coronavirus disease Coronavirus disease 2019 Coronavirus disease 2019 (COVID-19) COVID-19 Delta delta variant epithelial cells Evidence Infection infections less Mild symptom nasal epithelial cell nasal epithelium NEC NECs omicron pediatric replicate replicated Replication respiratory SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significantly lower Support susceptible These data vaccination Viral RNA virus VOCs widespread [DOI] 10.1371/journal.pbio.3001728 PMC 바로가기 [Article Type] Article
Major Complex Trait for Early De Novo Programming ‘CoV-MAC-TED’ Detected in Human Nasal Epithelial Cells Infected by Two SARS-CoV-2 Variants Is Promising to Help in Designing Therapeutic StrategiesCommunication Published on 2021-11-262022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] ADH5 aerobic fermentation analyses Analysis anti-SARS-CoV-2 anti-viral diagnosis and strategies artificial intelligence methodology Cell cell cycle cell cycle progression complex Critical data entry de novo defined disease severity E2F1 help Human immunology immunology paradigm shift in vitro Major melatonin microbiota nasal epithelial cell Nasal Epithelial cells performed platform primary target cell profile programming released repurposing drugs response SARS-CoV-2 SARS-CoV-2 Δ382 SARS-CoV-2 helicase SARS-COV-2 infection SARS-CoV-2 RdRp SARS-CoV-2 variant standardized protocol Strategy tested Transcriptome variant virus virus attack virus variant [DOI] 10.3390/vaccines9121399 PMC 바로가기 [Article Type] Communication
SARS-CoV-2 inhibition using a mucoadhesive, amphiphilic chitosan that may serve as an anti-viral nasal spray항 바이러스 비강 스프레이로서 작용할 수있는 점막 흡착성, 양친 매성 키토산을 사용한 SARS-COV-2 억제Article Published on 2021-10-082022-08-31 Journal: Scientific Reports [Category] SARS, 치료기술, 치료제, [키워드] A549 ACE-2 receptor adverse effect Anti-viral binding Biomedical materials Brain Cell clinical testing Concentration controls Coronavirus infections Course COVID-19 COVID-19 pandemic detect dose dosed effective electrostatic epithelial cells expressing Good host cells human airway human airway epithelial human airway epithelial cells hypothesise Infection inhibit injected limit log mammalian mechanism mice nares nasal nasal epithelial cell open Polymer Practice Prevent preventive intervention Prophylactic RATs reduce respiratory respiratory droplet Respiratory droplets respiratory tract Safe SARS-CoV-2 Spread spread of COVID-19 statistical significance TCID transgenic mice Vero E6 Vero E6 cell VERO E6 cells Viral viral entry viral infection Viral load virus [DOI] 10.1038/s41598-021-99404-8 PMC 바로가기 [Article Type] Article
Ethacridine inhibits SARS-CoV-2 by inactivating viral particlesResearch Article Published on 2021-09-032022-10-29 Journal: PLoS Pathogens [Category] COVID-19, [키워드] Air-liquid interface antiprotease antiviral activity assays binding BSL2 caused cell types cells coronavirus coronavirus SARS-CoV-2 COVID-19 demonstrated drug drugs effective FDA-approved drug host cells identify immunofluorescence inactivation inhibit inhibiting inhibitor living cell MPro nasal epithelial cell other disease plaque powerful tool Protease inhibitor Respiratory disease RT-PCR SARS-CoV-2 screened Stage viral infection viral particle Viral particles [DOI] 10.1371/journal.ppat.1009898 PMC 바로가기 [Article Type] Research Article
Immunohistochemical and qPCR Detection of SARS-CoV-2 in the Human Middle Ear Versus the Nasal Cavity: Case Series인간 중이 대 비강에서 SARS-CoV-2의 면역조직화학 및 qPCR 검출: 케이스 시리즈Case Reports Published on 2021-08-282022-09-10 Journal: Head and Neck Pathology [Category] COVID19(2023년), MERS, SARS, 치료기술, [키워드] ACE2 Adhesion Molecule angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Biomarker Cell cell adhesion molecule colonize control patients coronavirus COVID-19 COVID-19 patient detection disorder ear EpCAM epithelia epithelial epithelial cell epithelial cells evaluate Evidence expression hearing loss histologic Human immunohistochemistry implicated Infection middle Middle ear mucosa nasal Nasal cavity nasal epithelial cell Nasal Epithelial cells nasal tissue nasal tissue samples otitis otitis media pathophysiology Post-mortem qPCR Quantitative Quantitative RT-PCR receptor required RT-PCR SARS-CoV-2 SARS-CoV-2 entry sensorineural hearing loss Sery Spread Viral viral infections Viral load [DOI] 10.1007/s12105-021-01378-6 PMC 바로가기 [Article Type] Case Reports
Cytokine-Induced Modulation of SARS-CoV2 Receptor Expression in Primary Human Nasal Epithelial CellsArticle Published on 2021-07-052022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] ACE2 ACE2 receptor acute respiratory syndrome change Chronic rhinosinusitis contribute coronavirus CRS Cytokines downregulated dysregulated expression host cell Human human nasal epithelial cells immune indicate inflammatory state mucosa nasal nasal epithelial cell nasopharynx patients SARS-CoV2 SARS-CoV2 infection Support susceptibility swabbing symptoms of COVID-19 Th1 Th2 Th2 cytokine the spike protein TMPRSS2 TMPRSS2 expression Transmission upper respiratory tract viral replication virus [DOI] 10.3390/pathogens10070848 PMC 바로가기 [Article Type] Article
Fluticasone Propionate Suppresses Poly(I:C)-Induced ACE2 in Primary Human Nasal Epithelial Cells플루티카손 프로피오네이트는 1차 인간 비강 상피 세포에서 폴리(I:C) 유도 ACE2를 억제합니다Cellular and Infection Microbiology Published on 2021-04-262022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, [키워드] ACE2 ACE2 expression ACE2 protein Activation Affect affected agonist analyses angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 cell entry collected Corticosteroid COVID-19 dissemination enzyme epithelial cells evaluate examined expression expression of gene fluticasone fluticasone propionate Fold change followed by Gene Expression Health health problem HNECs host cells Human immunoblot immunofluorescence incubated Incubation individuals Infection initial innate immune interferon intranasal intranasal steroid spray investigated mRNA expression nasal Nasal cavity nasal epithelial cell nasal mucosa NFκB objective poly(I:C poly(I:C) promote propionate protease Protein quantitative PCR receptor regulated required reservoir Result SARS-CoV-2 SARS-CoV-2 cell SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 viral SARS-CoV2 Serine serine 2 significantly increased Spread steroid suppresse suppressed thought TLR TLR agonists TLR3 TMPRSS2 Toll-like receptors transmembrane transmembrane protease serine 2 Viral virus infection [DOI] 10.3389/fcimb.2021.655666 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Inhibition of acid sphingomyelinase by ambroxol prevents SARS-CoV-2 entry into epithelial cellsResearch Article Published on 2021-04-232022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, [키워드] ACE2, angiotensin-converting enzyme 2 acid sphingomyelinase Activation acute respiratory syndrome addition ambroxol applied ATCC, American Type Culture Collection cells cellular ceramide clinical study coronavirus COVID-19, Coronavirus disease 2019 DMEM, Dulbecco's modified Eagle's medium DTPA, diethylenetriaminepentaacetic acid eGFP, enhanced GFP epithelial cell epithelial cells event FCS, fetal calf serum FIASMAs, functional inhibitors of the acid sphingomyelinase fluorescence microscopy H/S, Hepes/saline human nasal epithelial cells Infection infection with SARS-CoV-2 inhalation inhibit inhibition MEM, minimum essential medium mimicking nasal epithelial cell NP-40, Nonidet P40 OGP, n-octyl-d-glucopyranoside Particle PFA, paraformaldehyde pp-VSV-SARS-CoV-2, vesicular stomatitis virus pseudoviral particles presenting SARS-CoV-2 spike protein on their surface presenting Prevent prevented purified quantified reduced required restored rhinovirus SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 spike protein SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 shown TMPRSS2, Transmembrane serine protease 2 vesicular stomatitis virus viruses Volunteer [DOI] 10.1016/j.jbc.2021.100701 PMC 바로가기 [Article Type] Research Article
Ex vivo assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells비강 상피 세포의 SARS-CoV-2 감염 예방에 있어 스핑고지질을 표적으로 하는 약물의 효능을 평가하기 위한 생체 외 분석Article Published on 2021-03-192022-08-31 Journal: STAR protocols [Category] MERS, SARS, 신약개발, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 amitriptyline antidepressant approved assays Cell isolation Cell-based assays. Complete Coronavirus-2 COVID-19 drug drugs targeting Efficacy epithelial cell epithelial cells evaluate Ex vivo Infection infection level infection levels inhibitor nasal epithelial cell offer Particle presenting protocol quantified Replication respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sphingolipid Spike protein stomatitis the SARS-CoV-2 vesicular stomatitis vesicular stomatitis virus Volunteer were infected [DOI] 10.1016/j.xpro.2021.100356 PMC 바로가기 [Article Type] Article