Integrated High-Throughput Centrifugal Microfluidic Chip Device for Pathogen Detection On-SiteArticle Published on 2024-06-192024-09-05 Journal: Biosensors [Category] update2024, [키워드] centrifugal microfluidic chip Coriolis force Device hydrophobic valve LAMP nucleic acid extraction paraffin valve siphon valve [DOI] 10.3390/bios14060313 PMC 바로가기 [Article Type] Article
Absolute Quantification of Hepatitis B Core Antigen (HBcAg) Virus-like Particles and Bound Nucleic AcidsArticle Published on 2023-12-212024-09-05 Journal: Viruses [Category] update2024, [키워드] analytical characterization gene delivery HBcAg nucleic acid extraction RP-HPLC silica spin column virus-like particles [DOI] 10.3390/v16010013 PMC 바로가기 [Article Type] Article
Rapid, sensitive, and specific, low-resource molecular detection of Hendra virusarticle Published on 2023-02-102024-09-05 Journal: One Health [Category] update2024, [키워드] Hendra virus isothermal amplification N gene nucleic acid amplification test nucleic acid extraction Nucleic acid lateral flow point-of-care Rapid test recombinase polymerase amplification [DOI] 10.1016/j.onehlt.2023.100504 PMC 바로가기 [Article Type] article
An external quality assessment for the molecular testing of the SARS-CoV-2 virus genome in Zhejiang Province, ChinaArticle Published on 2022-11-012022-11-15 Journal: Diagnostic microbiology and infectious disease [Category] COVID19(2023년), SARS, 진단, [키워드] Accuracy Asymptomatic CDC China Combination Concentration concentrations Concordance COVID-19 COVID-19 pandemic Culture diagnostics external quality assessment false-negative False-positive Genome infected individual Laboratory medium molecular molecular diagnostics negative sample nucleic acid extraction organized participated PCR Population positive sample provide public health rapid expansion reagents SARS-CoV-2 SARS-CoV-2 test SARS-CoV-2 tests serial dilution the SARS-CoV-2 virus viral titer Zhejiang [DOI] 10.1016/j.diagmicrobio.2022.115766 PMC 바로가기
Validation Study of a Direct Real-Time PCR Protocol for Detection of Monkeypox VirusArticle Published on 2022-11-012022-11-15 Journal: The Journal of molecular diagnostics : JMD [Category] 치료기술, [키워드] Accuracy approach coronavirus disease COVID-19 described detection develop Direct eliminating imperative IMPROVE International Laboratory Laboratory testing Monkeypox nucleic acid extraction outbreak pandemic PCR protocol public health emergency Real-time PCR reducing sensitivity The United States validation virus World Health Organization [DOI] 10.1016/j.jmoldx.2022.09.001 PMC 바로가기
Development and application of LAMP assays for the detection of enteric adenoviruses in fecesArticle Published on 2022-08-312022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] acute gastroenteritis Adenovirus amplification Asymptomatic carried children clinical sample colorimetric detection Combination comparable Comparative Complete detect Detection limit development diagnostic diagnostic capability diagnostics DNA DNA extraction electrophoretic separation equipment false-positive result faster fluorescent Fluorescent probe gastroenteritis gold standard Hepatitis human adenoviruses 40 and 41 identify Infection infections intestinal isothermal Laboratory LAMP Loop-mediated isothermal amplification many cases microorganisms molecular molecular diagnostics nucleic acid extraction Nucleic acid material outcomes pathogen pathogen detection Patient PCR reduce reported resources SARS-CoV-2 sensitivity sensitivity of PCR Severe case severity specificity threshold viral loads virus [DOI] 10.1128/spectrum.00516-22 PMC 바로가기
High-Throughput COVID-19 Testing of Naso-Oropharyngeal Swabs Using a Sensitive Extraction-Free Sample Preparation MethodArticle Published on 2022-08-312022-11-15 Journal: Microbiology Spectrum [Category] SARS, 치료기술, [키워드] approach calibrated Cobas costly COVID-19 detection system diagnosis of SARS-CoV-2 diagnostic Direct direct method downstream extraction-free extraction-free method gold standard High-throughput Infection International lack less log medium naso-oropharyngeal swab nasopharyngeal swab specimen nucleic acid extraction offer ORF1ab outcome PCR PCR assay per day PlexPCR pool positive sample preparation proteinase protocol purification quantification RdRP reagent reagents reduction in required Respiratory Coronavirus reverse transcription-PCR RNA Roche Sample SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitive sensitivity tested Testing time-consuming Transport Treatment Viral load Viral RNA WHO [DOI] 10.1128/spectrum.01358-22 PMC 바로가기
Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)역전사 루프 매개 등온 증폭(RT-LAMP)을 사용한 SARS-CoV-2 면봉 샘플에 대한 임상 테스트Article Published on 2022-08-182022-09-11 Journal: BMC Infectious Diseases [Category] SARS, 진단, 치료기술, [키워드] 95% CI Care change clinical clinical testing Diagnosis diagnostic Efficiency end point equipment isothermal amplification Laboratory limit Nasopharyngeal swab Nasopharyngeal swab samples nucleic acid nucleic acid extraction pH sensitive indicator. phenol Phenol red positive reaction RNA extraction RNA extraction kit RT-LAMP SARS-CoV-2 SARS-CoV-2 swab sample sensitive indicator sensitivity specificity was used [DOI] 10.1186/s12879-022-07684-w PMC 바로가기 [Article Type] Article
RT-qPCR and ATOPlex sequencing for the sensitive detection of SARS-CoV-2 RNA for wastewater surveillance폐수 감시를 위한 SARS-CoV-2 RNA의 민감한 검출을 위한 RT-qPCR 및 ATOPlex 시퀀싱Article Published on 2022-07-152022-09-11 Journal: Water research [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 appear approach assays assessment China CDC Combination Concentration Concentration method condition coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 deep sequencing detect detection Detection limit detection rate Detection sensitivity enveloped virus greater handling impacted less limit of detection MONITOR multiplex Mutation N1 and N2 nucleic acid nucleic acid extraction offer ORF1ab pandemic PCR platform powerful tool Probability promise quantitation quantitative PCR recovery required Result reverse transcription RT-qPCR RT-qPCR assay RT-qPCR assays SARS-CoV-2 SARS-CoV-2 RNA Sequencing severe acute respiratory syndrome Coronavirus shown Spread Surveillance US CDC variants variants of concern viral genome virus VoC wastewater [DOI] 10.1016/j.watres.2022.118621 PMC 바로가기 [Article Type] Article
Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settingsCDC 2019 SARS-CoV-2 분석을 사용한 직접 PCR: 제한된 리소스 설정에 대한 최적화Article Published on 2022-07-112022-09-12 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] amplification annealing temperature CDC clinical samples COVID-19 COVID-19 pandemic demonstrated diagnostics Direct Effect elevated evaluated extraction step Factor maintain methodology N2 set nucleic acid extraction offer overcome pathogen Patient PCR positive protocol qRT-PCR Reactions reported resource SARS-CoV-2 sensitivity specificity specimen viral transport media Volume VTM widespread [DOI] 10.1038/s41598-022-15356-7 PMC 바로가기 [Article Type] Article