Temporal dynamics of SARS-CoV-2 genome and detection of variants of concern in wastewater influent from two metropolitan areas in ArkansasArticle Published on 2022-11-252022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Affect approach Arkansas association Asymptomatic changes in Community complementary contribute coronavirus COVID-19 COVID-19 case COVID-19 testing death detect diagnostic Factors fecal genetic diversity Genetic variant genomic change highlight identity infected individual investigated nucleocapsid ORF1ab polymerase chain polyprotein public health quantified Quantitative recorded RNA sequencing RT-qPCR RT-qPCR assay SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 mutations SARS-CoV-2 RNA SARS-CoV-2 variant sensitivity silent Support Surface glycoprotein Surveillance TaqMan assay target Temporal the disease transmission of SARS-CoV-2 variants variants of concern viral gene viral titer virus virus genome was used wastewater Wastewater. [DOI] 10.1016/j.scitotenv.2022.157546 PMC 바로가기
Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2Article Published on 2022-11-152022-10-06 Journal: Sens Actuators B Chem [Category] 진단, [키워드] acute respiratory syndrome amplification approach caused coronavirus COVID-19 COVID-19 pandemic cross-contamination Detection limit diagnostics Dumbbell-type triplex molecular switch Epidemics Frame gold standard High-fidelity independent Logic-gate molecular Molecular assay N gene nucleic acid amplification ORF1ab probe recognizing Region RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 RNA. spacer [DOI] 10.1016/j.snb.2022.132579 PMC 바로가기 [Article Type] Article
Wastewater-based epidemiological surveillance to monitor the prevalence of SARS-CoV-2 in developing countries with onsite sanitation facilitiesArticle Published on 2022-10-152022-11-15 Journal: Environmental pollution (Barking, Essex : 1987) [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] absence approach collected Complete Contagion correlation country COVID-19 COVID-19 pandemic Critical detect Developing developing countries disease drain epidemiological Epidemiology facilitate genetic component genetic material help highest identification investigated Isolation MONITOR N genes National National surveillance program Onsite sanitation ORF1ab outbreak pandemic positive Prevalence reported RT-qPCR Rural Hotspot based sampling sampling SARS-CoV-2 SARS-CoV-2 monitoring SARS-CoV-2 RNA service Surveillance urban Urban Drain based sampling viral copy number Wastewater-based epidemiology (WBE). were used [DOI] 10.1016/j.envpol.2022.119679 PMC 바로가기
A novel vaccine based on SARS-CoV-2 CD4+ and CD8+ T cell conserved epitopes from variants Alpha to OmicronArticle Published on 2022-10-062022-11-15 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] 100% conserved Analysis Antigen Beta caused CD4 CD4+ CD8 CD8+ T cell clade clades composed confirmed cases conserved COVID-19 cross-protective deaths Delta epitope Epitopes Epsilon Eta Gamma Genome genomes GISAID greater highly antigenic HLA binding hydrophobic IFN-γ IL-10 IL-12 IL-2 IL-6 immune response immune responses immunoinformatics in silico approaches in vivo Lambda long-lasting majority memory Metadata molecular docking Mortality Mutation non-toxic offer omicron ORF ORF1ab ORF8 proteins peptide performed promoted Protein reduced Reinfection SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 variants secretion severity Strains suffered T cell epitope TGF-β Th1 the Spike the vaccine TLR4 Vaccine variant variants virus virus transmission WHO Wuhan [DOI] 10.1038/s41598-022-21207-2 PMC 바로가기
RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiationSARS-CoV-2 RNA 검출 및 변이체 분화를 위한 다중 형광 프로브를 결합한 RT-LAMP 분석Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition applied can be used clinical setting clinical settings detect Fluorescent probes Genes isothermal amplification limit of detection Loop-mediated isothermal amplification molecular mutant Mutation N gene observation ORF1 ORF1ab P681H P681R positive result primer set probe quantification regions reverse transcription Reverse transcription loop-mediated isothermal amplification (RT-LAMP) RT-LAMP S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 viral RNA sensitivity simple specificity Spread Transcription type strain variant Variant differentiation. viral RNA detection virus wild type wild type strain wild-type [DOI] 10.1016/j.talanta.2022.123644 PMC 바로가기 [Article Type] Article
Whole-genome sequencing of SARS-CoV-2 reveals diverse mutations in circulating Alpha and Delta variants during the first, second, and third waves of COVID-19 in South Kivu, east of the Democratic Republic of the CongoResearch article Published on 2022-09-012022-10-05 Journal: International Journal of Infectious Diseases [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] A.23.1 Analysis B.1.351 B.1.617.2 circulating circulation coding sequence consensus genome COVID-19 D614G Delta delta variant Diagnosis disease severity dominant Genes Genetic evolution Genetic variation genome coverage genomic global pandemic highlight identify include information isolate Lineage management mapped molecular MOST Mutation nucleocapsid protein objective ORF1ab ORF3 outbreak Oxford P.1 pandemic pangolin Phylogenetic analysis Point mutation processed reference genome responsible Result reveal SARS-CoV-2 SARS-CoV-2 isolate sequence sequenced Sequencing Seven South South Kivu subsequent Surveillance synonymous the SARS-CoV-2 genome Treatment variant variants were used whole-genome sequencing Wuhan [DOI] 10.1016/j.ijid.2022.05.041 [Article Type] Research article
Mutational dynamics across VOCs in International travellers and Community transmission underscores importance of Spike-ACE2 interaction국제 여행자의 VOC와 지역 사회 전파에 걸친 돌연변이 역학은 Spike-ACE2 상호 작용의 중요성을 강조합니다.Article Published on 2022-09-012022-09-11 Journal: Microbiological research [Category] COVID19(2023년), SARS, 변종, [키워드] A570D Alpha analysed B.1.617.2 binding binding affinity change Community community transmission comparable COVID-19 case COVID-19 pandemic D1118H D950N Delta demonstrated different time point different time points disequilibrium early phase emergence Evolution functional G142D Genomic surveillance India Interaction International investigated L452R Lineage linkage Linkage disequilibrium Mutation mutation analysis mutations N501Y notable omicron ORF1 ORF1ab overlap predict raised S982A SARS-CoV-2 selective spike spike mutations spike-ACE2 Spread Structural analysis Transmissibility Transmission transmission pattern unique vaccination variants variants of concern. VoC VOCs [DOI] 10.1016/j.micres.2022.127099 PMC 바로가기 [Article Type] Article
SARS-CoV-2 transmission from infected owner to household dogs and cats is associated with food sharingArticle Published on 2022-09-012022-11-15 Journal: International Journal of Infectious Diseases [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] Active surveillance addition animal anthropogenic association cats collected coronavirus country COVID-19 COVID-19 pandemic dogs Ecuador food Food sharing gene target IMPROVE Interaction Latin Latin America nasal swab oral ORF1ab organization pets polymerase chain positive Quantitative reported reverse transcription risk factor Risk factors risk of transmission SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 surveillance SARS-CoV-2 test SARS-CoV-2 transmission SARS-CoV-2. Sequencing shown Spread statistically significant Support tested Total Transmission transmission of SARS-CoV-2 was performed whole genome yielding [DOI] 10.1016/j.ijid.2022.05.049 PMC 바로가기
Detection of SARS-CoV-2 in different human biofluids using the loop-mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil루프 매개 등온 증폭 분석을 사용하여 다양한 인간 생체 유체에서 SARS-CoV-2 검출: 브라질 Fortaleza의 전향적 진단 연구Article Published on 2022-09-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] 95% CI 95% confidence interval Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adopted biofluid Brazil caused Chain Reaction collected coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 detect detection Diagnosis diagnostic evaluated gold gold standard human gene isothermal amplification LAMP Loop-mediated isothermal amplification management Molecular diagnosis Molecular diagnosis. Nasopharyngeal swab nasopharyngeal swabs ORF1 ORF1ab oropharyngeal swabs pandemic Patient polymerase chain polymerase chain reaction primer primer set Primers processed Quantitative quantitative polymerase chain reaction RT-LAMP RT-qPCR SARS-CoV-2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus specificity [DOI] 10.1002/jmv.27842 PMC 바로가기 [Article Type] Article
COVID salivary diagnostics: A comparative technical studyCOVID 타액 진단: 비교 기술 연구Article Published on 2022-09-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 assumption can be used Care Chain Reaction coronavirus coronavirus disease Coronavirus disease 2019 COVID COVID-19 Delta diagnostic diagnostics direct methods eliminating envelope golden standard heat heat-released RNA high accuracy indicate individuals infected individual infected individuals Intervention Isolation mass mass surveillance molecular molecular diagnostics Mouthwash nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs noninvasive NPs Open reading frame open reading frame 1 open reading frame 1ab ORF1ab pandemic patients polymerase chain polymerase chain reaction promote remained reported respiratory restriction RNA RNA extraction robust RT-PCR Saliva Saliva. salivary sampling SARS-CoV-2 sensitivity severe acute respiratory syndrome Coronavirus specificity Surveillance the patient Viral viral gene viral genes virus spread while widespread [DOI] 10.1002/jmv.27883 PMC 바로가기 [Article Type] Article