Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system원핵생물 발현 시스템에서 기능적으로 활성인 재조합 SARS-CoV-2(COVID-19) 3C 유사 프로테아제 및 가용성 불활성 3C 유사 프로테아제-RBD 키메라의 생산Article Published on 2022-06-202022-09-11 Journal: Epidemiology and Infection [Category] COVID19(2023년), SARS, 치료제, [키워드] 3C-like protease acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antigenicity approach blocked carrier cells Chimer chimeric chymotrypsin co-expressed complex coronavirus COVID-19 COVID-19 vaccines cysteine cysteine protease cysteine proteases dimeric disease drug ELISAs enzyme Escherichia coli Escherichia coli BL21 exhibit expression function inactive inhibitors inhibitory main protease non-structural protein non-structural proteins Papain Papain-like protease PLPro polyproteins pp1a pp1ab processing produced Production prokaryotic expression system protease Proteases Protein purification RBD Receptor binding domain RTC SARS-CoV-2 Serine serum severe acute respiratory syndrome Coronavirus solubility Spike protein target tested the Spike Transcription vaccinated individual vaccinated individuals viral replication [DOI] 10.1017/S0950268822001078 PMC 바로가기 [Article Type] Article
[Nirmatrelvir plus ritonavir (Paxlovid) a potent SARS-CoV-2 3CLpro protease inhibitor combination][니르마트렐비르와 리토나비르(팍슬로비드)의 강력한 SARS-CoV-2 3CLpro 프로테아제 억제제 조합]Review Published on 2022-06-012022-09-11 Journal: Revista española de quimioterapia : publicación [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] 3CL 3CLpro Administered adverse effects Antiviral antiviral drug antiviral drugs antiviral efficacy approved average conserved coronavirus Coronaviruses death duration of treatment effective Effectiveness EMA English FDA Final Hospitalization IMPROVE indican inhibitory activity media metabolism Modification Nirmatrelvir NSP11 Pandemia pandemic Patient Paxlovid PF-07321332 polyproteins pp1a pp1ab processing protease Protease inhibitor Proteases Proteins reducing risk Ritonavir SARS-CoV-1 SARS-CoV-2 sequence shown Spanish therapeutic target Treatment Viral variants [DOI] 10.37201/req/002.2022 PMC 바로가기 [Article Type] Review
Detection of SARS-CoV-2 Proteins in Wastewater Samples by Mass Spectrometry질량 분석법에 의한 폐수 샘플의 SARS-CoV-2 단백질 검출Article Published on 2022-04-192022-09-11 Journal: Environmental science & technology [Category] COVID19(2023년), SARS, 치료기술, [키워드] Biomarker Chain Reaction collected COVID-19 COVID-19 infection COVID-19 pandemic detection eight Epidemiology feces health system host cell Infection information liquid chromatography−mass spectrometry mass mass spectrometry mass vaccination occurred outbreak peptide peptides polymerase chain polymerase chain reaction pp1ab Protein proteomics quantification Quantitative quantitative polymerase chain reaction Sample SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins SARS-CoV-2 RNA SARS-CoV-2 virus Spectrometry the SARS-CoV-2 virus These data transcribed Treatment unique Urine vaccination wastewater [DOI] 10.1021/acs.est.1c04705 PMC 바로가기 [Article Type] Article
A Computer-Aided Approach for the Discovery of D-Peptides as Inhibitors of SARS-CoV-2 Main ProteaseMolecular Biosciences Published on 2022-01-242022-10-31 Journal: Frontiers in Molecular Biosciences [Category] COVID-19, [키워드] 3CL pro 3CLpro approach Area caused cleavage conducted conserved cysteine protease D-peptide drug candidate drug target enzymatic assay Free energy calculation highlighting identify in silico Approach inhibiting inhibitor Mechanics molecular Molecular dynamics simulation nonstructural protein polyprotein pp1a pp1ab problem protease protease active site Protein Replication reported responsible SARS-CoV-2 SARS-CoV-2 main protease structures subsequent surface therapeutic agents Virtual screening virus [DOI] 10.3389/fmolb.2021.816166 PMC 바로가기 [Article Type] Molecular Biosciences
Regulation of the Dimerization and Activity of SARS-CoV-2 Main Protease through Reversible Glutathionylation of Cysteine 300Research Article Published on 2021-08-172022-10-29 Journal: mBio [Category] COVID-19, MERS, SARS, [키워드] activity acute respiratory syndrome bat coronaviruses Cell chymotryptic digestion cleavage coronavirus coronavirus disease COVID-19 cysteine cysteines demonstrated dimerization drug targets ENCODE experiment gel filtration glutaredoxin glutathionylation inhibit M pro main protease Modification monomer mutant oxidative stress pathophysiology polyprotein polyproteins pp1a pp1ab protease Protein provide reactive reducing regulated Replication required responsible restored Reversible SARS-CoV-2 susceptible therapeutic interventions thioltransferase ultracentrifugation undergo viral replication [DOI] 10.1128/mBio.02094-21 PMC 바로가기 [Article Type] Research Article
The SARS-CoV-2 Programmed −1 Ribosomal Frameshifting Element Crystal Structure Solved to 2.09 Å Using Chaperone-Assisted RNA CrystallographyArticles Published on 2021-07-302022-10-28 Journal: ACS chemical biology [Category] COVID-19, SARS, [키워드] Affect alteration base pair chaperone conformation conserved contribute cryo-EM crystal Crystallography disrupt drug drug design drug target element Frame function genomes High-resolution junctions nonstructural protein observé polyprotein pp1a pp1ab produced promote provide RNA SARS-CoV-2 sequence stability stop codon Structure three-dimensional structure translation viral life cycle viral replication [DOI] 10.1021/acschembio.1c00324 PMC 바로가기 [Article Type] Articles
Effect of genome composition and codon bias on infectious bronchitis virus evolution and adaptation to target tissuesResearch Article Published on 2021-04-072022-10-28 Journal: BMC Genomics [Category] Coronavirus, [키워드] accessory protein accessory proteins acting Affect affecting appear bronchitis calculated Cell coding codon Codon Bias codon usage Codons Complete contribute CpG demonstrated downloaded Effect effective Evidence Evolution expressed gene expression level Factor Genome Genome composition genomes help Host IBV increase in Infectious Bronchitis Virus innate immune response investigated involved nucleotide Odds ratio phenotype polyprotein pp1a pp1ab Protein Proteins Regulation Result selective sequence significant difference strain structural protein supplementary material synonymous target tissue target tissues tissues virus without affecting [DOI] 10.1186/s12864-021-07559-5 PMC 바로가기 [Article Type] Research Article
Role of Structural and Non-Structural Proteins and Therapeutic Targets of SARS-CoV-2 for COVID-19COVID-19에 대한 SARS-CoV-2의 구조적 및 비구조적 단백질과 치료 표적의 역할Review Published on 2021-04-062022-09-11 Journal: Cells [Category] MERS, SARS, 신약개발, 진단, 치료법, [키워드] 2019-nCoV accessory protein accessory proteins ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 Amino acid angiotensin angiotensin converting enzyme 2 Angiotensin-converting enzyme angiotensin-converting enzyme 2 bats cats causative agent cellular entry cleavage components consequence convalescent plasm Coronaviridae coronavirus coronavirus disease Coronavirus disease-19 Coronavirus-2 COVID-19 death downstream drugs encapsulate event family focus functional Genome genomic genomic RNA greater gRNA host cell host cell receptor hosts humans Infection lead mammalian MERS MERS-CoV molecular molecular function molecular target myriad non-structural protein Novel coronavirus polymerase polyproteins positive-sense pp1a pp1ab protease proteolytic cleavage RBD Receptor binding domain referred to replicase respiratory resulting RNA genome role S protein SARS-CoV SARS-CoV-2 SARS-COV-2 infection sequence identity Serine serine 2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sgRNAs similarity single-stranded Spike protein spike protein of SARS-CoV-2 Spread ssRNA structural protein structural proteins subgenomic mRNA subgenomic mRNAs synthesis target therapeutic therapy TMPRSS2 translated translation transmembrane transmembrane protease serine 2 undergo Vaccine Variability Viral Viral RNA virus [DOI] 10.3390/cells10040821 PMC 바로가기 [Article Type] Review
Spatial and temporal roles of SARS-CoV PL pro -A snapshotSARS-CoV PL pro -A 스냅샷의 공간적 및 시간적 역할Review Published on 2021-01-012022-09-11 Journal: FASEB journal : official publication of the Federa [Category] 치료법, [키워드] 3CL pro accessory protein accessory proteins Antiviral appear autocleavage C-terminus can not cleave cleaved CoV replication cysteine cysteine protease dilemma domain E3 ubiquitin ligase envelope Factor inhibit inhibitor innate immune response Interaction interferon interferon-stimulated gene ISG15 its target protein its target proteins IκBα membrane motif NF-κB nonstructural protein nsp1 Nsp3 Nsps Nucleocapsid proteins p53 Papain Papain-like protease PLPro polyprotein polyproteins pp1a pp1ab promote protease Protein provide RCHY1 released Replication SARS-CoV SARS-CoV-2 SARS-CoV-2 infections stimulator STING TNF ubiquitin unique Viral Zinc [DOI] 10.1096/fj.202002271 PMC 바로가기 [Article Type] Review
Unpacking Pandora from Its Box: Deciphering the Molecular Basis of the SARS-CoV-2 CoronavirusReview Published on 2020-12-312022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, MERS, SARS, [키워드] 2019-nCoV Basis bats cellular coronavirus coronavirus 2 COVID-19 COVID-19 pandemic Diagnosis drug feature glycosylation site Host molecular non-structural proteins novel open reading frames ORF1ab pandemic Pandora Pneumonia polyprotein pp1a pp1ab Repository respiratory RNA SARS-CoV-2 Scientific community seeking self-cleavage sequence shielding spike trimer the SARS-CoV-2 translational Transmission unique Unpacking Vaccine viral genomes virus [DOI] 10.3390/ijms22010386 PMC 바로가기 [Article Type] Review