An ultra-sensitive one-pot RNA-templated DNA ligation rolling circle amplification-assisted CRISPR/Cas12a detector assay for rapid detection of SARS-CoV-2Article Published on 2023-03-012024-09-05 Journal: Biosensors & bioelectronics [Category] update2024, [키워드] CRISPR/Cas12a operator RCA RNA-Templated DNA ligation SARS-CoV-2 [DOI] 10.1016/j.bios.2023.115179 PMC 바로가기 [Article Type] Article
A Dual-Encoded Bead-Based Immunoassay with Tunable Detection Range for COVID-19 Serum EvaluationArticle Published on 2022-09-122022-11-15 Journal: Angewandte Chemie (International ed. in English) [Category] 진단, [키워드] antigens Asymptomatic case C-reactive protein Circle Concentration Coronavirus 2019 COVID-19 COVID-19 antibody detection Disease progression help identify immunoassay Inflammatory biomarker interleukin-1β interleukin-6 magnitude Microfluidic Immunoassays multiplex Multiplexing nucleocapsid Patient procalcitonin Prognosis Range RCA Receptor binding domain Rolling Rolling circle amplification sensitivity serological treat Tunable Detection Range. [DOI] 10.1002/anie.202203706 PMC 바로가기
Collection and detection of SARS-CoV-2 in exhaled breath using face mask안면 마스크를 사용하여 호기에서 SARS-CoV-2 수집 및 감지Article Published on 2022-08-182022-09-11 Journal: PLoS ONE [Category] SARS, 치료기술, 치료제, [키워드] addition breath collected Collection Cross-infection electrostatic examined expected face Face mask Face masks Genes Mask Masks Molecular tests Nasopharyngeal swab Nasopharyngeal swab samples noninvasive nucleic acid ORF1 ORF1ab pandemic PCR PCR assay PCR assays PCR test PCR tests positive Prevent PROTECT protocol quantity RCA RdRP reduce RNA extraction Rolling circle amplification SARS-CoV-2 SARS-CoV-2 test SARS-CoV-2 tests significantly subjects target gene Target genes Transmission utility virus virus detection was performed was selected [DOI] 10.1371/journal.pone.0270765 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 RNA through tandem isothermal gene amplification without reverse transcription역전사 없이 탠덤 등온 유전자 증폭을 통한 SARS-CoV-2 RNA 검출Article Published on 2022-06-152022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] Accuracy alternative amplification amplified Analysis cDNA complex composed COVID-19 COVID-19 epidemic Cycling detect detection diagnose Diagnosis DNA emission form G-quadruplex gene amplification generate hairpin structure isothermal Isothermal gene amplification linear primer Primers produced RCA repeated reverse transcription Rolling Rolling circle amplification SARS-COV-2 infection SARS-CoV-2 RNA SARS-CoV-2 viral SARS-CoV-2 viral RNA sensitivity single-stranded DNA ssDNA strand Strand displacement amplification subsequent Thioflavin Thioflavin T. viral genes Viral RNA [DOI] 10.1016/j.aca.2022.339909 PMC 바로가기 [Article Type] Article
Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an exampleReview article Published on 2022-06-012022-10-05 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] advance amplification Bio-detection chain Characteristics Circle clinical settings Combination COVID-19 COVID-19 pandemic Diagnosis diagnostics Efficiency example feature greater limitations Molecular diagnosis NAAT nucleic acid amplification Nucleic acid amplification technologies offer PCR POCT point-of-care point-of-care diagnostics RCA reaction REcombinase Research Rolling RPA SARS-CoV-2 sensitivity Sensitivity and specificity specificity stability unique [DOI] 10.1016/j.bios.2022.114109 [Article Type] Review article
Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticlesArticle Published on 2022-02-182022-10-29 Journal: Nature Communications [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] AuNP Bioanalytical chemistry biosensors current DNA DNA and RNA DNA nanostructures exhibiting experiment Gold nanoparticle Nanoparticles nucleic acid overcome RCA Rolling circle amplification SARS-CoV-2 RNA simple Spectroscopy [DOI] 10.1038/s41467-022-28627-8 PMC 바로가기 [Article Type] Article
A novel isothermal method for amplification of long specific amplicon from linear templateArticle Published on 2022-02-172022-10-29 Journal: Scientific Reports [Category] COVID-19, [키워드] amplification analyzed applied Biological techniques can be used Cre recombinase CREA diagnostic DNA downstream evaluated generate genomic isothermal limit linear nucleic acid amplification Pathogens RCA Recombination RNA RNA templates SARS-CoV-2 spike sequence subsequent Surveillance Variation [DOI] 10.1038/s41598-022-06785-5 PMC 바로가기 [Article Type] Article
Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping통합 미세 시스템에서의 롤링 서클 증폭 : 대규모 다중 다중 병원체 진단 및 유전자형 분석을 향한 미세한 보석Review Published on 2021-10-122023-05-28 Journal: Accounts of Chemical Research [Category] Coronavirus, Fulltext, MERS, SARS, 변종, 유전자 메커니즘, 진단, 치료기술, 치료제, [키워드] amplification Analysis Antibiotic resistance antibiotic resistance gene antimicrobial resistance Automation Bacterial base pair biomedical engineering Biotechnology Circle clinical laboratories diagnostics dissect example exploration facilitate focus fungal genetic material genotyping gold gold standard green hand handling human disease include infected cell infected cells integration intrinsically isothermal isothermal amplification mechanism miniaturization molecular multiomics analysis NAAT nucleic acid nucleic acid amplification nutshell opens opportunity path pathogen Pathogenesis Pathogens PLP point-of-care probe RCA reported Research resistance genes robust Rolling Rolling circle amplification RT-PCR SARS-CoV-2 pandemic seamless sensitivity sequence Signal transduction spatial information specificity Streamline subject the reader threat tissue translate translation Vaccines variety Viral viral variant [DOI] 10.1021/acs.accounts.1c00438 PMC 바로가기 [Article Type] Review
Padlock probe-based rolling circle amplification lateral flow assay for point-of-need nucleic acid detection필요 시점 핵산 검출을 위한 자물쇠 프로브 기반 롤링 서클 증폭 측면 유동 분석Article Published on 2021-06-282022-09-11 Journal: The Analyst [Category] SARS, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome amplification Analysis antimicrobial resistance Bacterial binding site biosafety can be used cleave complex complexity Culture diagnostics digestion DNA DNA and RNA double-stranded environmental monitoring Epidemiology faster food safety help inherent isothermal isothermal amplification method isothermal amplification methods lateral flow lateral flow immunoassay limit limit of detection linear NAAT Novel coronavirus Nuclease nucleic acid nucleic acid amplification tests Nucleic acid detection padlock pathogen Pathogens PCR plan platform polymerase probe provide RCA reaction referred to resistance gene respiratory robustness Rolling circle amplification Saliva SARS-CoV-2 sensitive Severe acute respiratory syndrome severe acute respiratory syndrome-coronavirus-2 significantly single-stranded DNA targets Therapeutics tissue extract turnaround time water [DOI] 10.1039/d1an00399b PMC 바로가기 [Article Type] Article
Barriers Influencing Vaccine Development Timelines, Identification, Causal Analysis, and Prioritization of Key Barriers by KOLs in General and Covid-19 Vaccine R&DPublic Health Published on 2021-04-202022-10-31 Journal: Frontiers in Public Health [Category] COVID-19, MERS, SARS, [키워드] affected Analysis barrier benefit can be used Causal cause causes concerning COVID-19 vaccine development cycle development Difficult disease high risk highest identification impacted impair Innovation knowledge KOLs lack pathogen Pathogens preclinical study ranking of innovation barriers RCA respond response ROI target timeline timelines Vaccine Vaccine development vaccine development timelines Vaccines [DOI] 10.3389/fpubh.2021.612541 PMC 바로가기 [Article Type] Public Health