On-Site and Visual Detection of the H5 Subtype Avian Influenza Virus Based on RT-RPA and CRISPR/Cas12aArticle Published on 2024-05-102024-09-05 Journal: Viruses [Category] update2024, [키워드] CRISPR/Cas12a H5 AIVs RT-RPA Visual detection [DOI] 10.3390/v16050753 PMC 바로가기 [Article Type] Article
Rapid detection of avian influenza virus based on CRISPR-Cas12aarticle Published on 2023-11-132024-09-05 Journal: Virology Journal [Category] update2024, [키워드] Avian influenza virus CRISPR/Cas system detection RT-RPA [DOI] 10.1186/s12985-023-02232-7 PMC 바로가기 [Article Type] article
Rapid and Visual Detection of SARS-CoV-2 RNA Based on Reverse Transcription-Recombinase Polymerase Amplification with Closed Vertical Flow Visualization Strip Assayarticle Published on 2023-01-092024-09-05 Journal: Microbiology Spectrum [Category] update2024, [키워드] amplification detection RT-RPA SARS-CoV-2 Viral RNA Visual [DOI] 10.1128/spectrum.02966-22 PMC 바로가기 [Article Type] article
iSCAN-V2: A One-Pot RT-RPA–CRISPR/Cas12b Assay for Point-of-Care SARS-CoV-2 DetectionBioengineering and Biotechnology Published on 2022-01-212022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] amplification biosensing clinical contagious diseases COVID-19 CRISPR-Cas systems, Cas12b CRISPR-Dx Critical cross-contamination detection diagnostic Factors feature inherent isothermal amplification isothermal amplification method less molecular Nucleic acid detection palindromic repeat pathogen detection Patient PCR platform POC POC Dx point-of-care polymerase Protein Rapid Reactions risk RT-qPCR RT-RPA SARS-CoV-2 SARS-CoV-2 detection sensitivity specificity Spread temperature [DOI] 10.3389/fbioe.2021.800104 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
Evaluation of recombinase-based isothermal amplification assays for point-of-need detection of SARS-CoV-2 in resource-limited settingsArticle Published on 2021-11-072023-06-15 Journal: International Journal of Infectious Diseases [Category] MERS, SARS, [키워드] Diagnosis Point-of-need REcombinase RT-RAA RT-RPA SARS-CoV-2 [DOI] 10.1016/j.ijid.2021.11.007 PMC 바로가기 [Article Type] Article
Single- and Two-Stage, Closed-Tube, Point-of-Care, Molecular Detection of SARS-CoV-2SARS-CoV-2의 1단계 및 2단계, 폐쇄관, 현장 진료, 분자 검출Article Published on 2021-09-282022-09-11 Journal: Analytical Chemistry [Category] SARS, 치료기술, 치료제, [키워드] Amplicon amplicons amplification analytical sensitivity carried Chain Reaction COVID-19 pandemic Cycling described detection diagnostic test effective first stage fluorescent Instrumentation Isolation isothermal amplification LAMP loop mediated isothermal amplification molecular N gene nasopharyngeal swabs not need nucleic acid ORF1ab oropharyngeal swabs Patient PCR assays polymerase chain polymerase chain reaction primer processed provide quantitative polymerase chain reaction reagent real time Region reverse transcription RNA RT-LAMP RT-qPCR RT-RPA Saliva SARS-CoV-2 SARS-CoV-2 test sensitivity Support tested threshold Vaccine Viral load viral loads virus virus genome [DOI] 10.1021/acs.analchem.1c03016 PMC 바로가기 [Article Type] Article
Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification AssayArticle Published on 2021-09-252022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] 3′ untranslated region amplification Analysis canine conserved coronavirus decrease detection Detection limit develop diagnostic DNA amplification effort Endemic evaluate facilitate Fecal sample Feline coronavirus Feline infectious peritonitis FIP gastroenteritis identify Infection isothermal amplification Laboratory nucleic acid Pathogens performed point-of-need testing polymerase Population real-time RT-PCR REcombinase recombinase polymerase amplification resulting reverse transcription RNA RT-PCR RT-RPA running Sensitivity and specificity temperature tested Testing viral burden virus [DOI] 10.3390/pathogens10101237 PMC 바로가기 [Article Type] Article
Detection of Infectious Viruses Using CRISPR-Cas12-Based AssayArticle Published on 2021-08-282022-10-27 Journal: Biosensors [Category] COVID-19, [키워드] acute respiratory syndrome Amplicon amplification anticipated applicability cause change cleavage complex Concentration coronavirus Coronavirus disease-19 COVID-19 COVID-19 patients CRISPR-Cas12 detection fluorescent gRNA Infectious disease infectious viruses isothermal lateral flow MERS-CoV Middle East nasal swab outbreak palindromic repeat POCT point-of-care polymerase probes produced Protein public health reliability respiratory syndrome coronavirus reverse transcription RNA RT-RPA SARS-CoV SARS-CoV-2 sensitivity specificity sputum virus viruses [DOI] 10.3390/bios11090301 PMC 바로가기 [Article Type] Article
Detection of Infectious Viruses Using CRISPR-Cas12-Based AssayArticle Published on 2021-08-282022-10-27 Journal: Biosensors [Category] Coronavirus, COVID-19, SARS, [키워드] acute respiratory syndrome Amplicon amplification anticipated applicability cause change cleavage complex Concentration coronavirus Coronavirus disease-19 COVID-19 COVID-19 patients CRISPR-Cas12 detection fluorescent gRNA Infectious disease infectious viruses isothermal lateral flow MERS-CoV Middle East nasal swab outbreak palindromic repeat POCT point-of-care polymerase probes produced Protein public health reliability respiratory syndrome coronavirus reverse transcription RNA RT-RPA SARS-CoV SARS-CoV-2 sensitivity specificity sputum virus viruses [DOI] 10.3390/bios11090301 PMC 바로가기 [Article Type] Article
Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2SARS-CoV-2의 다중화된 CRISPR 기반 진단을 위한 자율적인 랩온페이퍼Article Published on 2021-07-132022-09-11 Journal: Lab on a chip [Category] SARS, 진단, 치료기술, [키워드] Accuracy amplification autonomous caused clinical sample comparable coronavirus COVID-19 COVID-19 infection COVID-19 pandemic CRISPR detect develop diagnosis of SARS-CoV-2 diagnostics early diagnosis Infectious disease Infectious diseases Internal control molecular multiplex other infectious diseases pandemic paper platform polymerase public health emergency Rapid respiratory Respiratory Coronavirus reverse transcription RT-PCR RT-RPA SARS-CoV-2 SARS-CoV-2 virus severe acute respiratory coronavirus Spread Swab Viral Viral RNA [DOI] 10.1039/d1lc00293g PMC 바로가기 [Article Type] Article