ACE2 decoy receptor generated by high-throughput saturation mutagenesis efficiently neutralizes SARS-CoV-2 and its prevalent variants고처리량 포화 돌연변이 유발에 의해 생성된 ACE2 미끼 수용체는 SARS-CoV-2 및 그 유행하는 변이체를 효율적으로 중화합니다Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 binding ACE2-RBD binding approach binding binding affinity C-terminal cellular receptor changes in convalescent sera Critical critical point D614G decoy receptor domain Effect fused global pandemic glycosylation site High-throughput screening increase in Microarray monoclonal antibodies Mutagenesis mutations neutralization neutralize neutralized Omicron variant Point mutation prevalent protease Protein protein engineering Pseudotyped virus RBD RBD binding RBD-ACE2 receptor Receptor binding domain rescued SARS-CoV-2 SARS-CoV-2 virus significantly single-cell soluble ACE2 Spike protein the SARS-CoV-2 the SARS-CoV-2 virus therapeutic potential Treatment Vaccine variant variants viral entry viral spike protein wild-type [DOI] 10.1080/22221751.2022.2079426 PMC 바로가기 [Article Type] Article
Converting non-neutralizing SARS-CoV-2 antibodies into broad-spectrum inhibitorsArticle Published on 2022-11-012022-11-15 Journal: Nature chemical biology [Category] SARS, 변종, 진단, [키워드] ACE2 acute respiratory syndrome addition angiotensin-converting enzyme 2 antibody binding component conserved coronavirus defined Diseases epitope Epitopes highlighting inhibitor inhibitors inhibitory linker monoclonal neutralize Neutralizing activity non-neutralizing antibody omicron pandemic receptor SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 evolution SARS-CoV-2 variant target the SARS-CoV-2 Treatment viral spike protein VOCs [DOI] 10.1038/s41589-022-01140-1 PMC 바로가기
Multiplex Hybrid Antigen-Capture LC-MRM Quantification in Sera and Nasal Lining Fluid of AZD7442, a SARS-CoV-2-Targeting Antibody CombinationArticle Published on 2022-11-012022-11-15 Journal: Analytical Chemistry [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료제, [키워드] antibodies antibody applicability approach binding capture reagent clinical analysis Combination complementarity-determining region conditions coronavirus disease Critical demonstrated domain extension Final hybrid implementation Infection Ligand limit lining mAb macromolecules Magnetic beads matrix effect monoclonal antibody multidimensional nasal offer patient population peptide performed platform quantification reagent reagents reproducibility required sensitivity serum significantly Tandem mass spectrometry Treatment vaccination viral spike protein [DOI] 10.1021/acs.analchem.2c01320 PMC 바로가기
An antibody that neutralizes SARS-CoV-1 and SARS-CoV-2 by binding to a conserved spike epitope outside the receptor binding motifArticle Published on 2022-10-282022-11-15 Journal: Science Immunology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] ACE2 receptor acute respiratory syndrome antibody Beta binding conserved coronavirus cryo-EM structure Delta delta variant demonstrated develop DNA epitope Evolution glycosylation site identify immune lung mice neutralize neutralizing activities omicron RBD Receptor binding domain receptor binding motif reduced SARS Coronavirus SARS coronaviruses SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2-specific B cell sequenced single-cell the RBD therapeutic variant Viral load viral spike protein were infected [DOI] 10.1126/sciimmunol.abp9962 PMC 바로가기
Deep mutational learning predicts ACE2 binding and antibody escape to combinatorial mutations in the SARS-CoV-2 receptor-binding domainArticle Published on 2022-10-132022-11-15 Journal: Cell [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] ACE2 ACE2 binding antibody antibody treatment artificial intelligence COVID-19 COVID-19 pandemic deep deep learning deep sequencing develop directed evolution domain Evolution of SARS-CoV-2 machine learning mutated variant Mutation mutations Neutralizing antibodies neutralizing antibody omicron predict Predictive Protein protein engineering RBD RBD variants representing SARS-CoV-2 variant Selection sequence the SARS-CoV-2 therapeutic Trajectories Vaccine variant variants viral escape viral spike protein yeast yeast display [DOI] 10.1016/j.cell.2022.08.024 PMC 바로가기
Antioxidant, Anti-inflammatory, and Immunomodulatory Roles of Nonvitamin Antioxidants in Anti-SARS-CoV-2 TherapyArticle Published on 2022-10-132022-11-15 Journal: Journal of medicinal chemistry [Category] COVID19(2023년), SARS, 바이오마커, 치료제, [키워드] ACE2 receptor Activation activities alleviate Alleviating alveolar antioxidant antiviral role conformational change COVID-19 Cytokine storm Dipeptidyl peptidase 4 Direct dosage Endothelium favor furin Helicase host receptor hyperinflammation Illness severity immunogenic Inflammatory Inflammatory responses influences membranes Organ failure oxidative stress Papain-like protease pathology pathway protease reactive oxygen specy Receptor binding domain Replication risk RNA-dependent RNA polymerase role serine 2 the spike protein transmembrane vicious cycle viral spike protein [DOI] 10.1021/acs.jmedchem.2c01134 PMC 바로가기
Histologic, viral, and molecular correlates of heart disease in fatal COVID-19Research article Published on 2022-10-012022-10-05 Journal: Annals of diagnostic pathology [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] ACE2 expression activated arrhythmia Arrhythmias cardiac connexin 43 Control COVID-19 disease dysfunction edema endocytosed Endothelial cell evidenced experiment expression fatal COVID-19 greater heart heart tissue histologic host response IL6 immunohistochemistry In situ hybridization induce interstitial Macrophage manifestation Migration molecular multifocal myocarditis Myocardium Myocyte nucleocapsid protein obesity perivascular predominant Protein Proteins quantified risk RNA SARS-CoV-2 spike protein SARS-CoV2 severe COVID-19 sodium Spike protein the spike protein tissue TNFα Type II Diabetes unique Viral protein viral spike protein would increase [DOI] 10.1016/j.anndiagpath.2022.151983 [Article Type] Research article
Peimine inhibits variants of SARS-CoV-2 cell entry via blocking the interaction between viral spike protein and ACE2Article Published on 2022-10-012022-11-16 Journal: Journal of food biochemistry [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 acute respiratory syndrome addition Analysis binding affinity block Calu-3 caused cell entry complementary coronavirus Coronavirus disease 2019 COVID-19 COVID-19 patients docking drug energy transfer evade exhibit Fritillaria functional glycoprotein Hydrogen bond Infection inhibit inhibit SARS-CoV-2 inhibitor Interaction lung cells molecular docking analysis N501Y patients peimine. Protein SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 viral infection significantly small molecule suggested suppresse the spike protein treatment for COVID-19 Vaccine variant variant spike variants of concern variants of SARS-CoV-2 viral entry viral infection viral infections viral spike viral spike protein VoC VOCs [DOI] 10.1111/jfbc.14354 PMC 바로가기
Comparison of Intracellular Transcriptional Response of NHBE Cells to Infection with SARS-CoV-2 Washington and New York StrainsArticle Published on 2022-09-202022-11-15 Journal: Frontiers in Cellular and Infection Microbiology [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] 24 hour acute respiratory syndrome affected analyzed bioinformatics caused Cell cellular coronavirus COVID-19 D614G mutation Data mining death DEGs different time point differentially expressed gene dysregulated Effect epithelial experiment Gene ontology Genome global pandemic GO terms Host host cells host response Host transcriptional response host-virus hpi identify immune IMPROVE include Infection infections inhibited Intracellular mechanism Mutation New York NHBE pathway Pathways Patient positive Post-infection reported response resulting RNA sequencing data SARS-CoV-2 SARS-CoV-2 isolate severe disease shown Signaling signaling pathways similarity stability strain Strains These data transcript translation viral isolate viral spike protein Virology virus virus replication Washington while Wuhan, China [DOI] 10.3389/fcimb.2022.1009328 PMC 바로가기
Cellular and Humoral Immune Responses to SARS-CoV-2 Vaccination in Inflammatory Bowel Disease PatientsArticle Published on 2022-09-082022-11-15 Journal: Journal of Crohn's & colitis [Category] SARS, 진단, 치료기술, [키워드] analysed anti-nucleocapsid antibody anti-SARS-CoV-2 vaccine anti-spike antibody antibody baseline bowel cellular Cohort cohorts Concentration controls COVID-19 decrease functional humoral IBD individual Inflammatory Inflammatory bowel disease inhibitor Necrosis no difference nucleocapsid antigen Patient patients positive recipient released repeated response SARS-CoV-2 SARS-CoV-2 IgG SARS-CoV-2 IgG antibody SARS-CoV-2-specific T-cell response Seven simple T-lymphocyte the healthy Treatment vaccinated individual vaccination Vaccine vaccine dose vaccinee viral spike protein was performed were measured [DOI] 10.1093/ecco-jcc/jjac048 PMC 바로가기