Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detectionSARS-CoV-2 검출을 위한 오픈 소스 RNA 추출 및 RT-qPCR 방법Research Article Published on 2021-02-032022-08-13 Journal: PLoS ONE [Category] Biochemistry, MERS, 치료기술, 치료법, [키워드] addition adoption clinical sample commercial TaqPath master mix Community correlation COVID-19 pandemic COVID-19 testing described diagnostic effective evaluate evaluated facilitated Fluorescence imaging Infection isopropanol isopropanol precipitation less limit of detection nasopharyngeal NP swab opening positive protocol purified RNA qPCR thermocycler reaction reduce reopening risk RNA copy RNA extraction RT-qPCR RT-qPCRs SARS-CoV-2 detection SARS-CoV-2 testing second shown Swab swab samples TaqPath master mix Viral Viral RNA widespread [DOI] 10.1371/journal.pone.0246647 PMC 바로가기 [Article Type] Research Article
High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1 -based plasmid vectorEEF1A1 기반 플라스미드 벡터에 의한 안정적으로 형질감염된 CHO 세포에서 단량체 SARS-CoV-2 S 단백질 RBD 320-537의 고수준 발현Research Article Published on 2021-02-022022-08-13 Journal: PLoS ONE [Category] Biochemistry, COVID-19, [키워드] Accuracy acute respiratory syndrome Alpha amplification analyzed Antigen antigenic antigenic determinants C-terminal Cell culture cell line Chinese hamster CHO cell condition coronavirus coronavirus 2 Coronaviruses described determinant domain EEF1A1 elongation factor exceeded except for expression gel gel filtration Genetic Genome glycans glycosylation Host immune response inducer mammalian mass spectrometry methotrexate monomeric N-glycosylation peptide performed Plasmid PNGase F polyacrylamide polyacrylamide gel produced Protein purification RBD RBD protein recombinant Recombinant SARS-CoV-2 RBD reducing regulatory sequence regulatory sequences respiratory S protein S proteins SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 S protein selected serological serological test Serological tests severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 signal peptide subunit Subunit vaccine the S protein transfected cells translation unique Vaccine variant Viral viral envelope viral signal was obtained [DOI] 10.1371/journal.pone.0242890 PMC 바로가기 [Article Type] Research Article
Metabolomic/lipidomic profiling of COVID-19 and individual response to tocilizumabCOVID-19의 대사체/지질체 프로파일링 및 토실리주맙에 대한 개별 반응Research Article Published on 2021-02-012022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, MERS, 진단, [키워드] absence Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 affecting age age- and sex-matched controls alteration Analysis analyzed approach biochemical caused change changes in characterized Clinical data clinical manifestations Clinical symptoms Control control group controls coronavirus disease Coronavirus-2 correlation correlations COVID-19 COVID-19 pandemic COVID-19 patient COVID-19 patients determinant EDTA evaluated First wave Florence form global health Health heterogeneity humanized monoclonal antibody induce Infection intensive care treatment interleukin-6 interleukin-6 receptor Italy Magnetic resonance spectroscopy metabolite metabolome metabolomic metabolomics MONITOR NMR-based metabolomic and lipidomic profiling Novel coronavirus novel coronavirus disease nuclear nuclear magnetic resonance open outcomes pandemic pathophysiological mechanism Patient patients patients hospitalized plasma plasma-EDTA sample plasma-EDTA samples provide recombinant humanized monoclonal antibody respiratory response to treatment SARS-CoV-2 SARS-COV-2 infection Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 small-molecule profile subset Symptom the disease Tocilizumab tocilizumab administration Treatment treatment with tocilizumab world population [DOI] 10.1371/journal.ppat.1009243 PMC 바로가기 [Article Type] Research Article
A drug repurposing screen identifies hepatitis C antivirals as inhibitors of the SARS-CoV2 main protease약물 용도 변경 화면은 C형 간염 항바이러스제가 SARS-CoV2 주요 프로테아제의 억제제임을 확인합니다Research Article Published on 2021-02-012022-08-13 Journal: PLoS ONE [Category] Biochemistry, COVID-19, [키워드] Antiviral antiviral drug antiviral drugs appear boceprevir ciluprevir Compound demonstrated dose drug Drug development Hepatitis hepatitis C Human IC50 identify in vitro inhibit inhibitor inhibitors of SARS-CoV-2 lead MPro narlaprevir protease SARS-CoV-2 SARS-CoV-2 main protease SARS-CoV-2 Mpro SARS-CoV2 main protease screened selective telaprevir therapeutic repurposing validation study Viral [DOI] 10.1371/journal.pone.0245962 PMC 바로가기 [Article Type] Research Article
Switched and unswitched memory B cells detected during SARS-CoV-2 convalescence correlate with limited symptom durationSARS-CoV-2 회복기 동안 감지된 전환 및 전환되지 않은 메모리 B 세포는 제한된 증상 지속 기간과 상관 관계가 있습니다Research Article Published on 2021-01-282022-08-13 Journal: PLoS ONE [Category] Biochemistry, 진단, 치료기술, [키워드] activated acute disease acute respiratory syndrome acute respiratory syndrome coronavirus antibody antibody-secreting plasmablasts B cell B cell response B cell responses B cell subsets causative agent CD24 CD27 characterized classical Clinical outcome composed convalescent Convalescent patients convalescent plasma treatment Coronavirus-2 correlated COVID-19 Critical disease Donor donors duration of symptom enrolled expansion Frequency health emergency identify IGD IgG1 IgM IgM response IgM responses memory memory B cell memory B cells pandemic Peripheral blood plasma antibody positive RBD Receptor binding domain remained respiratory Respiratory illness response responses SARS-CoV-2 SARS-CoV-2 immunity SARS-CoV-2 spike protein receptor binding domain seropositive subjects Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 significant negative correlation significantly Spike protein subject subjects subset symptom duration the SARS-CoV-2 while [DOI] 10.1371/journal.pone.0244855 PMC 바로가기 [Article Type] Research Article
Single cell resolution of SARS-CoV-2 tropism, antiviral responses, and susceptibility to therapies in primary human airway epithelium1차 인간 기도 상피에서 SARS-CoV-2 향성의 단일 세포 분해능, 항바이러스 반응 및 요법에 대한 감수성Research Article Published on 2021-01-282022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, 치료제, [키워드] airway epithelial cells airway epithelium Antiviral antiviral immunity antiviral responses antiviral therapies antiviral therapy array blocked Cell cell population cell type cell types cellular ciliated cell ciliated cells Ciliated epithelial cells contribute COVID-19 pathogenesis dominant element elements epithelial cell epithelial cell type epithelial cell types epithelial cells expressed Factor flow cytometry gene signatures heterogeneity heterogenous host protease host protease TMPRSS2 Human airway epithelium hyperinflammatory responses IL-6 induction infect infected cell infected cells Infection inhibited initial interferon interferon response ISG ISGs mediator outcome Pathogenesis predominant primary human airway Remdesivir required respiratory respiratory tract RNA-sequencing SARS-CoV-2 SARS-COV-2 infection secretory cell secretory cells Single Cell susceptibility susceptible therapy These cells TMPRSS2 Treatment Treatment response variety Viral viral replication viral tropism [DOI] 10.1371/journal.ppat.1009292 PMC 바로가기 [Article Type] Research Article
High-dose corticosteroid pulse therapy increases the survival rate in COVID-19 patients at risk of hyper-inflammatory response고용량 코르티코스테로이드 펄스 요법은 과염증 반응의 위험이 있는 COVID-19 환자의 생존율을 증가시킵니다Research Article Published on 2021-01-282022-08-13 Journal: PLoS ONE [Category] Biochemistry, COVID-19, [키워드] Analysis C-reactive protein can be used carried co-morbidities co-morbidity Corticosteroid corticosteroid pulse Course COVID-19 COVID-19 patient D-dimer decrease decreased mortality determine Dexamethasone Diagnostic Criteria effective intervention Efficacy ferritin following Future HDCPT High dose high dose corticosteroid pulse therapy hyper-inflammatory response identify IL-6 inclusion criteria increase increased survival initial Laboratory Lactate lactate dehydrogenase marker Methylprednisolone morbidities Mortality multivariate Cox regression Non-survivors objective observational study parameter Patient patients pro-inflammatory pro-inflammatory markers prospective observational study randomized clinical trial RCT Result risk statistically significant Stratification survival rate survival rates survivor Test the patient Therapies therapy to define treated [DOI] 10.1371/journal.pone.0243964 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cellsS1/S2 절단 부위에 돌연변이가 있는 SARS-CoV-2 변이체는 TMPRSS2 결핍 세포에서 증식하는 동안 시험관 내에서 생성됩니다Research Article Published on 2021-01-212022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, COVID-19, [키워드] alteration antivirals bind Cell cell lines cell tropism cleavage cleavage site cleaved Coronaviruses Critical de novo demonstrated direct fusion dominant entry pathway exhibited experiments facilitate generate Host host cell host cell receptor host protease in vitro inefficient infect motif mutant mutants Mutation performed plaque plaques polybasic Protein proteolytic cleavage researcher respiratory S gene S protein S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 propagation SARS-CoV-2 S gene SARS-CoV-2 variant SARS-CoV-2 variants screened Serine Severe acute respiratory syndrome severe acute respiratory syndrome-coronavirus-2 shown target cells the cell the S protein TMPRSS2 transmembrane serine protease Transmissibility type strain undergo Vaccine variant Vero Cell Vero cells Viral viral entry virus virus strains virus variants viruses wild-type strain [DOI] 10.1371/journal.ppat.1009233 PMC 바로가기 [Article Type] Research Article
Modeling the structure of the frameshift-stimulatory pseudoknot in SARS-CoV-2 reveals multiple possible conformersSARS-CoV-2에서 프레임 이동 자극 유사 매듭의 구조를 모델링하면 여러 가능한 일치 항목이 나타납니다.Research Article Published on 2021-01-192022-08-13 Journal: PLoS Computational Biology [Category] Biochemistry, COVID-19, [키워드] Analysis assist Author blind cause conformation conformations Consistency Control coronavirus COVID-19 COVID-19 pandemic determine drug target effort experiment followed by help inhibit inhibiting Interpretation junction junctions Ligand ligands modeling modulating molecular molecular dynamics not seen Nuclease pseudoknot relative expression reveal RNA SARS Coronavirus SARS-CoV-1 SARS-CoV-2 stimulatory Support the SARS-CoV-2 Therapeutics topologies treating COVID-19 triggered Viral Viral protein Viral proteins viral replication Viral RNA [DOI] 10.1371/journal.pcbi.1008603 PMC 바로가기 [Article Type] Research Article
The coronavirus proofreading exoribonuclease mediates extensive viral recombination코로나바이러스 교정 엑소리보뉴클레아제는 광범위한 바이러스 재조합을 매개합니다Research Article Published on 2021-01-192022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, MERS, 유전자 메커니즘, 진단, 치료제, [키워드] attenuation conserved coronavirus coronavirus exoribonuclease coronavirus proofreading exoribonuclease Coronavirus replication CoV CoV polymerase CoV recombination CoV replication CoVs Critical Culture defective viral genome determinant determinants Diversity DVGs ExoN Exoribonuclease Frequency generate highlight immune evasion implicated inactivation Infection inhibitor inhibitors loci maintain mechanism MERS MERS-CoV MHV MHV nsp14-ExoN molecular mechanism molecular mechanisms murine hepatitis virus novel coronaviruses nsp14 nsp14-ExoN nucleoside Nucleoside analogues outbreak pandemic Population Prevent produced Recombination recombination frequency recombination junctions Replication required responsible RNA RNA proofreading exoribonuclease RNA recombination RNA synthesis SARS-CoV-2 sequence sequences similarity sub-genomic mRNA sub-genomic mRNAs These data Viral viral recombination virulence while zoonotic CoVs [DOI] 10.1371/journal.ppat.1009226 PMC 바로가기 [Article Type] Research Article