Abstract
Probing the human proteome in tissues and biofluids such as plasma is attractive for biomarker and drug target discovery. Recent breakthroughs in multiplex, antibody-based, proteomics technologies now enable the simultaneous quantification of thousands of proteins at as low as sub fg/ml concentrations with remarkable dynamic ranges of up to 10-log. We herein provide a comprehensive guide to the methodologies, performance, technical comparisons, advantages, and disadvantages of established and emerging technologies for the multiplexed ultrasensitive measurement of proteins. Gaining holistic knowledge on these innovations is crucial for choosing the right multiplexed proteomics tool for applications at hand to critically complement traditional proteomics methods. This can bring researchers closer than ever before to elucidating the intricate inner workings and cross talk that spans multitude of proteins in disease mechanisms.
【저자키워드】 proteomics, proximity extension assay, aptamer, immunoassays, multiplex, antibody array, antibody microarray, single-molecule array, technology comparison, technology advances, 【초록키워드】 Biomarker, knowledge, complement, Proteins, Protein, plasma, mechanisms, proteome, quantification, disease, Concentration, disadvantage, tissue, Comparisons, researcher, recent, biofluid, cross talk, drug target discovery, Probing, 【제목키워드】 technology, Human, detection, Depth,