Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types하이드로겔 입자는 여러 샘플 유형에서 SARS-CoV-2 RNA의 검출을 향상시킵니다.Article Published on 2020-12-302022-08-31 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] average Biological techniques Biotechnology CDC Cycle threshold value diagnostic EUA hydrogel IMPROVE medium Microbiology nCoV nucleic acid nucleic acid extraction Particle Particles positive sample Qiagen real-time RT-PCR RNA extraction Saliva saliva samples SARS-CoV-2 SARS-CoV-2 RNA tested Transport transport medium Viral Viral load Viral RNA viral titer viral titers virus Volume [DOI] 10.1038/s41598-020-78771-8 PMC 바로가기 [Article Type] Article
Viral and Antibody Kinetics of COVID-19 Patients with Different Disease Severities in Acute and Convalescent Phases: A 6-Month Follow-Up Study급성 및 회복기의 다양한 질병 중증도를 가진 COVID-19 환자의 바이러스 및 항체 동력학: 6개월 추적 연구Article Published on 2020-12-012022-09-11 Journal: Virologica Sinica [Category] MERS, SARS, 진단, 치료법, [키워드] acute phase acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibody Antibody Response asymptomatic individual Asymptomatic individuals caused China Clinical features close contact Cohort coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus disease 2019 (COVID-19) COVID-19 COVID-19 patient Cycle threshold value Delayed disappearance disease severity Economy Health IgG IgM IgM antibodies IgM antibody Infection Kinetics median Mild Most patients nucleocapsid protein occurred onset of symptoms Patient patients with SARS-CoV-2 Protein reduction in respiratory RT-qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid Seroconversion seropositive serum antibody serum antibody response severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) severe disease Spread symptomatic cases Symptomatic patients symptoms onset the antibody the vaccine These data titre Viral Viral load Viral RNA Viral RNA shedding viral shedding [DOI] 10.1007/s12250-020-00329-9 PMC 바로가기 [Article Type] Article
Relationship of the cycle threshold values of SARS-CoV-2 polymerase chain reaction and total severity score of computerized tomography in patients with COVID 19코로나19 환자의 SARS-CoV-2 중합효소연쇄반응 주기역치와 전산화단층촬영의 총 중증도 점수의 관계Article Published on 2020-12-012022-09-11 Journal: International Journal of Infectious Diseases [Category] SARS, 치료기술, 치료제, [키워드] acute lung inflammation age both groups buffer calculated Care Chain Reaction changes Chest Chest CT Chest tomography Computerized tomography contagious COVID 19 COVID-19 COVID-19 patient COVID-19 patients Critical Ct value Ct values cycle threshold Cycle threshold value died early stage early stages evaluate evaluated Factor FIVE help Hospitalization Hospitalized hospitalized patients identify in both groups lung lobes median age Medical care Mortality Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nasopharynx nucleic acid older patient older patients Outpatient outpatients Patient patients performed polymerase chain polymerase chain reaction primer Primers probe probes quantified ranged RdRP RdRp gene relationship reverse transcriptase Reverse transcriptase polymerase chain reaction RT-PCR RT-PCR test RT-PCR tests SARS-CoV-2 SARS-CoV-2 RNA severity significantly higher Spread Total Total severity score transcriptase TSS Turkey Viral Viral load viral nucleic acid virus was related [DOI] 10.1016/j.ijid.2020.09.1449 PMC 바로가기 [Article Type] Article
Genetic diversity of SARS-CoV-2 and clinical, epidemiological characteristics of COVID-19 patients in Hanoi, Vietnam베트남 하노이에서 SARS-CoV-2의 유전적 다양성과 COVID-19 환자의 임상, 역학적 특성Research Article Published on 2020-11-172022-08-13 Journal: PLoS ONE [Category] MERS, SARS, 유전자 메커니즘, [키워드] accompanied Admission analysed Asia B.1.1 lineage Characteristics China circulating Cluster country COVID-19 case COVID-19 patient Cycle threshold value D614G diarrhoea disease severity dominant dry cough epidemiological Europe European European country Fever Genetic variant genomes hospitalisation Illumina Illumina Miseq Local male median median age MOST Mutation mutations NC_045512 New Nsp12 P323L Patient positive patient Prevalence reference sequence reflected Result Russia SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 RT-PCR sequence homology sequenced sore throat strain Strains Symptom transition Tropical Diseases variant viruses [DOI] 10.1371/journal.pone.0242537 PMC 바로가기 [Article Type] Research Article
Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCRDispatch Published on 2020-10-012022-10-31 Journal: Emerging Infectious Diseases [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome coronavirus coronavirus disease COVID-19 Cycle threshold value Diagnosis distribution initial mass pooling positive Rapid Real-time PCR respiratory respiratory infections reverse transcription PCR RT-PCR SAR-CoV-2 SARS SARS-CoV-2 SARS-related coronavirus Screening sensitivity Severe acute respiratory syndrome severe acute respiratory syndrome coronavirus 2 South Korea specimen specimen pooling specimens Surveillance tested upper viruses zoonoses [DOI] 10.3201/eid2610.201955 PMC 바로가기 [Article Type] Dispatch
Heat inactivation decreases the qualitative real-time RT-PCR detection rates of clinical samples with high cycle threshold values in COVID-19Research article Published on 2020-09-012022-10-05 Journal: Diagnostic microbiology and infectious disease [Category] SARS, 치료기술, [키워드] Accuracy Affect caused clinical sample clinical samples conducted Consensus COVID-19 COVID-19 pandemic Cycle threshold value decrease detection rate Diagnosis gold standard heat heat inactivation heat-inactivation inactivated inactivation Inpatient pathogenicity positive previous study protocol qualitative detection Qualitative real-time RT-PCR real-time RT-PCR RNA detection SARS-CoV-2 SARSCoV-2 significantly suspected patient throat swab Transmissibility urgency [DOI] 10.1016/j.diagmicrobio.2020.115109 [Article Type] Research article
Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020Rapid Communication Published on 2020-08-132022-10-31 Journal: Eurosurveillance [Category] COVID-19, Fulltext, MERS, [키워드] acute respiratory syndrome analysed Asymptomatic coronavirus coronavirus disease correlation COVID-19 cycle threshold Cycle threshold value Decline Duration England Infectious virus infectiousness Mild-to-moderate persist public health policy respiratory virus RT-PCR SARS-CoV-2 symptom onset symptomatic upper respiratory tract Viral load virus [DOI] 10.2807/1560-7917.ES.2020.25.32.2001483 PMC 바로가기 [Article Type] Rapid Communication
Comparison of hydroxychloroquine, lopinavir/ritonavir, and standard of care in critically ill patients with SARS-CoV-2 pneumonia: an opportunistic retrospective analysisResearch Published on 2020-07-112022-11-01 Journal: Critical Care [Category] COVID-19, [키워드] acute respiratory syndrome Admission changes in Characteristics Clinical data compassionate use Coronavirus-2 Critically ill patient critically ill patients Cycle threshold value demonstrated drug Effect Efficacy Escalation Extra-corporeal membrane oxygenation group groups Hydroxychloroquine in viral intensive care unit intubation Lopinavir/ritonavir Mortality no difference no significant difference occurred outbreak outcome Patient plasma Pneumonia Primary outcome proportion Randomized controlled trial renal replacement therapy required respiratory tracts Result Retrospective analysis SARS-CoV-2 secondary specific treatment Standard of care Support therapeutic options treated Treatment ventilator-free day Viral load Virological [DOI] 10.1186/s13054-020-03117-9 PMC 바로가기 [Article Type] Research
Suitability and Sufficiency of Telehealth Clinician-Observed, Participant-Collected Samples for SARS-CoV-2 Testing: The iCollect Cohort Pilot StudyOriginal Paper Published on 2020-06-252022-10-31 Journal: JMIR Public Health and Surveillance [Category] COVID-19, [키워드] Analysis Blood clinical collected COVID-19 Cycle threshold value DBS sample Diagnosis diagnostic enrolled home testing Identifier indicated Laboratory nucleic acid objective Oropharyngeal oropharyngeal swab pandemic participant performed pilot study quantification Quantitative report Respiratory Coronavirus Result returned RNase P Saliva Sample SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 testing serology serology test serology testing specimen specimens submission Support telehealth Testing utility [DOI] 10.2196/19731 PMC 바로가기 [Article Type] Original Paper
Presymptomatic SARS-CoV-2 Infections and Transmission in a Skilled Nursing FacilityOriginal Article Published on 2020-04-242022-11-01 Journal: The New England Journal of Medicine [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Asymptomatic Atypical Cluster conducted contributed coronavirus cough Cycle threshold value demonstrated died evaluated facility Fever first positive Hospitalized identify Infection intensive care median median time Mortality nasopharyngeal nucleotide Nursing Oropharyngeal participated polymerase chain positive positive test result presymptomatic presymptomatic resident Prevent Rapid resident Result rRT-PCR Sample SARS-CoV-2 SARS-COV-2 infection sequence sequenced Sequencing Shortness of breath specimen Spread Symptom symptomatic Symptoms tested Transmission transmission of SARS-CoV-2 typical symptom viral culture virus were recorded widespread [DOI] 10.1056/NEJMoa2008457 PMC 바로가기 [Article Type] Original Article