Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution면봉 풀링: 샘플 희석을 피하는 SARS-CoV-2의 대규모 RT-qPCR 스크리닝을 위한 새로운 방법Research Article Published on 2021-02-042022-08-13 Journal: PLoS ONE [Category] MERS, 유전자 메커니즘, 진단, [키워드] Analysis asymptomatic patient collected diagnostic Dilution false-negative false-negatives False-positive false-positives Laboratory methodology Paired analysis Patient pooling populations positive individuals Prevalence ranged reduction required RT-qPCR RT-qPCR assays SARS-CoV-2 screened Swab swabs Volume [DOI] 10.1371/journal.pone.0246544 PMC 바로가기 [Article Type] Research Article
A Neutralization Assay Based on Pseudo-Typed Lentivirus with SARS CoV-2 Spike Protein in ACE2-Expressing CRFK CellsArticle Published on 2021-02-022022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] 293T ACE2 acute respiratory syndrome angiotensin-converting enzyme 2 anti-SARS-CoV-2 anti-spike antibody Antisera antiviral inhibitor applied Cell coated with contribute convalescent coronavirus COVID-19 CrFK CRFK Cell Dilution effective embryonic kidney ENhance evaluate HEK293T highly pathogenic HIV host cell human convalescent sera IC50 IgA IgG IgM Immunoglobulin immunoglobulin A IMPROVE Infection inhibitor inhibitory concentration kidney lentivirus mAbs methodology monoclonal monoclonal antibody Neutralization assay neutralizing monoclonal antibody pseudo-typed lentivirus reagent required researcher SARS CoV-2 SARS-CoV-2 spike Spike protein Spread the SARS-CoV-2 virus Virus neutralization zoonotic [DOI] 10.3390/pathogens10020153 PMC 바로가기 [Article Type] Article
Is there scientific evidence of the mouthwashes effectiveness in reducing viral load in Covid-19? A systematic reviewReview Published on 2021-02-012022-10-29 Journal: Journal of Clinical and Experimental Dentistry [Category] COVID-19, [키워드] appear article articles base Care carried chlorhexidine clinical Concentration concentrations COVID-19 decrease Dilution effective Effectiveness Evidence human saliva hydrogen hydrogen peroxide in viral in vitro independent Intervention Material and Method MERS-CoV Mouthwash mouthwashe mouthwashes performed peroxide reduce reducing reduction Research Result review Saliva SARS-CoV selected Seven Support systematic review tested Viral load virucidal viruses [DOI] 10.4317/jced.57406 PMC 바로가기 [Article Type] Review
SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virusSARS-CoV-2 특이적 항체 및 중화 분석은 바이러스에 대한 광범위한 체액성 면역 반응을 나타냅니다Article Published on 2021-01-292022-09-10 Journal: Communications Biology [Category] 진단, 치료기술, 치료법, [키워드] against SARS-CoV antibodies antibody Antibody Response assays blocked convalescent plasma COVID-19 COVID-19 patient detectable development Dilution donors Effectiveness Hospitalized hospitalized patients Host humoral humoral immune response IgA IgG antibodies IgG antibody IgG1 individual Infectious-disease diagnostics NAb NAbs negative controls Neutralization assay neutralization assays neutralization titer neutralization titers Neutralizing antibodies neutralizing antibody nucleocapsid protein Other Outpatient outpatients Patient Protein pseudotyped lentiviruses pseudovirus public health question response SARS CoV SARS CoV-2 SARS-CoV spike protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 neutralization SARS-CoV-2 spike protein sensitivity specific IgG antibody specificity spike Spike protein subject the antibody response titers Translational research Vaccine development Vaccines virus was determined [DOI] 10.1038/s42003-021-01649-6 PMC 바로가기 [Article Type] Article
In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methodsArticle Published on 2021-01-282022-10-29 Journal: Scientific Reports [Category] COVID-19, [키워드] acute respiratory syndrome Ammonium chloride chlorinated Concentration Coronavirus-2 Dilution Effectiveness evaluate global pandemic heat inactivation Infection Laboratory pathogen Pathogens Quantitative required SARS-CoV SARS-CoV-2 selected virus [DOI] 10.1038/s41598-021-82148-w PMC 바로가기 [Article Type] Article
Protective Effect of Arbidol Against Pulmonary Fibrosis and Sepsis in MicePharmacology Published on 2021-01-272022-10-31 Journal: Frontiers in Pharmacology [Category] COVID-19, [키워드] Against alleviate antiviral drug Arbidol Compliance Complication COVID-19 Cytokine storm death Dilution Effect Epidemiology evaluate the effect fecal IL-6 immunology Inflammatory Injury liver lung Lung function murine New coronavirus Patient patients with COVID-19 Perspective pro-inflammatory factor promoted pulmonary pulmonary fibrosis reduced Research Sepsis Serum level severity significantly survival rate TNF-α Treatment [DOI] 10.3389/fphar.2020.607075 PMC 바로가기 [Article Type] Pharmacology
Performance of Nucleic Acid Amplification Tests for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Prospectively Pooled Specimens전향적으로 통합된 표본에서 중증 급성 호흡기 증후군 코로나바이러스 2의 검출을 위한 핵산 증폭 테스트의 성능Expedited Published on 2021-01-012022-09-10 Journal: Emerging Infectious Diseases [Category] SARS, 진단, 치료기술, 치료제, [키워드] acid acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Algorithm analytical sensitivity characterized coronavirus coronavirus 2 coronavirus disease Coronaviruses COVID-19 cycle threshold dependent on detection diagnostic screening programs Dilution distribution Effect Efficiency envelope gene Frame independent limit of detection nucleic acid nucleic acid amplification nucleic acid amplification tests Open reading frame open reading frame 1ab performance positive proportion prospectively pooled specimens ranged respiratory respiratory infections reverse transcription PCR SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specimen System Test tested Testing virus zoonoses [DOI] 10.3201/eid2701.203379 PMC 바로가기 [Article Type] Expedited
Multiplexed detection and quantification of human antibody response to COVID-19 infection using a plasmon enhanced biosensor platformResearch article Published on 2021-01-012022-10-05 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, [키워드] antibody Antibody binding Antigen approach binding interaction biosensor Blood correlated COVID-19 COVID-19 antibody tests COVID-19 infection diagnosing diagnostic tests Dilution Efficacy ELISA enzyme evaluated fluorescent human antibody response IgA IgG IgM immunoassay Immunoglobulin immunosorbent interactions isotypes Last linear measure microsphere multiplex nucleocapsid protein pandemic Patient plasmonic platform quantification Quantitative SARS CoV-2 sensitivity serum serum IgG serum sample target test result was used yielding [DOI] 10.1016/j.bios.2020.112679 [Article Type] Research article
Lack of Serologic Evidence of Infection Among Health Care Personnel and Other Contacts of First 2 Confirmed Patients With COVID-19 in Illinois, 20202020년 일리노이주에서 처음 2명의 COVID-19 확인된 환자의 의료 종사자 및 기타 접촉자 간의 감염에 대한 혈청학적 증거 부족Article Published on 2021-01-012022-09-11 Journal: Public health reports (Washington, D.C. : 1974) [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibodies antibody Antibody testing Asymptomatic Atypical Care Characteristics collected collected data Community condition Confirmed Contact Contact tracing coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 determine Dilution dilutions eligible enrolled enzyme-linked immunosorbent enzyme-linked immunosorbent assay Epidemiology Evidence evidence of exposure to Follow-up Health Health care Infection initial Interaction medium Other participant Patient Personal protective equipment PPE provided question questionnaire reported respiratory risk SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody testing SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 spike secondary transmission Seroconversion serologic serologic testing serology serum severe acute respiratory syndrome Coronavirus specimen symptom onset tested Transmission Transmission dynamics virus with COVID-19 [DOI] 10.1177/0033354920966064 PMC 바로가기 [Article Type] Article
Comparison of four commercial RT‐PCR diagnostic kits for COVID‐19 in ChinaResearch Article Published on 2020-12-152022-10-30 Journal: Journal of Clinical Laboratory Analysis [Category] COVID-19, SARS, [키워드] amplification curve approved China clinical Concentration coronavirus disease COVID‐19 COVID‐19 patient COVID‐19 patients COVID‐19 Detection limit detection rate diagnostic diagnostics Dilution dilutions drug evaluated examined expected feasible initial N gene Nasopharyngeal swab nasopharyngeal swab specimen Nasopharyngeal swab specimens nasopharyngeal swabs ORF1ab ORF1ab gene Patient performed RNA RT‐PCR RT‐PCR selected sensitivity Sensitivity and specificity specificity specimen sputum Sputum sample sputum samples Stage state Suspected target the disease [DOI] 10.1002/jcla.23605 PMC 바로가기 [Article Type] Research Article