The microglial NLRP3 inflammasome is involved in human SARS-CoV-2 cerebral pathogenicity: A report of three post-mortem cases소교세포 NLRP3 인플라마솜은 인간 SARS-CoV-2 대뇌 병원성에 관여합니다: 3건의 사후 사례 보고Case Reports Published on 2021-12-152022-09-11 Journal: Journal of neuroimmunology [Category] SARS, 진단, 치료기술, 치료법, 치료제, [키워드] ACE2 age astrocytes Brain CD68 cell entry receptor cortical COVID-19 COVID-19 cases Critical death Deceased Human Human brain human SARS-CoV-2 immunofluorescence Inflammasome involved lesion lung Macrophage macrophages microglia microglial neurons NLRP3 NLRP3 inflammasome nucleocapsid Post-mortem SARS-CoV-2 SARS-CoV-2 nucleocapsid the SARS-CoV-2 therapeutic target tissues Viral [DOI] 10.1016/j.jneuroim.2021.577728 PMC 바로가기 [Article Type] Case Reports
In-House Immunofluorescence Assay for Detection of SARS-CoV-2 Antigens in Cells from Nasopharyngeal Swabs as a Diagnostic Method for COVID-19Article Published on 2021-12-132022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] Accuracy against SARS-CoV antibodies antibody benefit Cell clinically COVID-19 COVID-19 case COVID-19 patient cross-reactivity demonstrated detection detection of SARS-CoV-2 Diagnosis Diagnostic method domain evaluate highest identify immunofluorescence immunofluorescence assay individuals infected individuals mice Microscopy monoclonal antibody multiplex immunofluorescence Nasopharyngeal swab nasopharyngeal swabs non-infected NPs nucleocapsid protein polyclonal antibody positive prevalence of COVID-19 primary antibody Protein raised respiratory epithelial cell respiratory epithelial cells respiratory viruses SARS-CoV SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 N protein SARS-CoV-2 nucleocapsid SARS-CoV-2 spike protein Sensitivity and specificity smear staining Surveillance tested the epidemic viral culture virus virus-infected cell was used were stained were used [DOI] 10.3390/diagnostics11122346 PMC 바로가기 [Article Type] Article
Association of the Serum Levels of the Nucleocapsid Antigen of SARS-CoV-2 With the Diagnosis, Disease Severity, and Antibody Titers in Patients With COVID-19: A Retrospective Cross-Sectional StudyMicrobiology Published on 2021-12-092022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Analysis Antibody titer Antigen automated chemiluminescence immunoassay clinical feature collected Coronavirus disease 2019 COVID-19 Cutoff cutoff value Diagnosis diagnosis of COVID-19 group IgG antibody titers Laboratory test Level moderate N antigen Non-COVID-19 nucleocapsid antigen Patient receiver operating characteristic SARS-CoV-2 SARS-CoV-2 nucleocapsid Sensitivity and specificity serum serum sample severe COVID-19 severity significant negative correlation significantly higher symptomatic patient the disease the patient the SARS-CoV-2 titer with mild disease [DOI] 10.3389/fmicb.2021.791489 PMC 바로가기 [Article Type] Microbiology
Nucleocapsid mutations R203K/G204R increase the infectivity, fitness, and virulence of SARS-CoV-2뉴클레오캡시드 돌연변이 R203K/G204R은 SARS-CoV-2의 감염성, 적합성 및 독성을 증가시킵니다Article Published on 2021-12-082022-09-11 Journal: Cell Host & Microbe [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, 치료법, [키워드] adaptive addition analyses association B.1.1.7 Combination competition contribute COVID-19 COVID-19 severity experiment fatality fitness Frequency hamsters human lung cell human lung cells in silico Infectivity Mutation nucleocapsid nucleocapsid protein observé pandemic Phylogenetic analysis positive Protein R203K/G204R replication advantage SARS-CoV-2 SARS-CoV-2 lineage SARS-CoV-2 nucleocapsid SARS-CoV-2 variants Spike protein the SARS-CoV-2 the spike protein Transmission variant variants variants of concern Viral virulence virus [DOI] 10.1016/j.chom.2021.11.005 PMC 바로가기 [Article Type] Article
SARS-CoV-2 N Protein Induces Acute Lung Injury in Mice via NF-ĸB ActivationSARS-CoV-2 N 단백질은 NF-ĸB 활성화를 통해 쥐의 급성 폐 손상을 유발합니다Immunology Published on 2021-12-072022-09-11 Journal: Frontiers in Immunology [Category] SARS, 치료법, [키워드] acute lung injury acute respiratory syndrome addition administration antibody blocked body fluid body fluids Bone marrow C57BL/6 cell count Contamination coronavirus disease Coronavirus disease 2019 COVID-19 Cytokines denaturation elevated expression in vitro induce Infection inhibitor Injury intratracheal administration lipopolysaccharide LPS lung Lung injury Macrophage macrophage polarization macrophages mice N-protein neutrophil neutrophil infiltration NF- kappa B nuclear nucleocapsid (N) protein nucleocapsid protein p65 pathway Phosphorylation Polymyxin B produced Proinflammatory cytokine proinflammatory cytokines promoted Protein PYRROLIDINE pyrrolidine dithiocarbamate recombinant reported respiratory Result SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 nucleocapsid therapeutic target therapeutic targets toxic Treatment triggered [DOI] 10.3389/fimmu.2021.791753 PMC 바로가기 [Article Type] Immunology
Long-term specific IgG response to SARS-CoV-2 nucleocapsid protein in recovered COVID-19 patients회수 된 CovID-19 환자에서 SARS-COV-2 뉴 클레오 캡시드 단백질에 대한 장기 특이 적 IgG 반응Article Published on 2021-12-012022-09-01 Journal: Scientific Reports [Category] SARS, 진단, 치료기술, 치료법, [키워드] absence acute respiratory syndrome acute respiratory syndrome coronavirus age anti-N IgG Blood blood sampling collected coronavirus coronavirus disease Coronavirus Disease (COVID)-19 correlated COVID-19 symptom IgG IgG response immune response immunoassay Immunoglobulin Immunoglobulin G Infection Infectious diseases Microparticle Patient Pneumonia positively correlated presence or absence Protein recovered COVID-19 patient Recovered COVID-19 patients respiratory respiratory specimen respiratory specimens SARS-CoV-2 detection SARS-CoV-2 nucleocapsid Seropositivity serum sample serum samples severe acute respiratory syndrome Coronavirus significantly lower specimens symptom onset Symptoms Vaccine Virology was measured [DOI] 10.1038/s41598-021-02659-4 PMC 바로가기 [Article Type] Article
Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion인간 SARS-CoV-2 혈청전환을 검출하기 위한 뉴클레오캡시드 단백질에 기초한 간접 ELISA의 항원 생산 및 개발Article Published on 2021-12-012022-09-11 Journal: Brazilian journal of microbiology : [publication o [Category] MERS, 진단, 치료법, [키워드] Accuracy controls COVID-19 COVID-19 case COVID-19 cases described detect determine develop ELISA ELISA kit enzyme-linked immunosorbent enzyme-linked immunosorbent assay Escherichia coli exposure to expressed head Head-to-head comparison helping homogeneity human SARS-CoV-2 humoral Humoral response identify immunization Immunological test mass production nucleocapsid protein Protein purified qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 nucleocapsid sensitivity Sensitivity and specificity sera Seroconversion serological assays the SARS-CoV-2 Vaccines was used [DOI] 10.1007/s42770-021-00556-6 PMC 바로가기 [Article Type] Article
Electrochemical Capillary-Flow Immunoassay for Detecting Anti-SARS-CoV-2 Nucleocapsid Protein Antibodies at the Point of Care치료 시점에서 항-SARS-CoV-2 뉴클레오캡시드 단백질 항체를 검출하기 위한 전기화학적 모세관 흐름 면역분석법Article Published on 2021-11-262022-09-11 Journal: ACS Sensors [Category] MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] Accuracy anti-N antibodies anti-N antibody antibody Blood samples board capillary-flow device carbon Combination Device Diagnosis diagnostic reliability Electrochemistry ELISA enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay hydrophilic IgG antibodies IgG antibody immunoassay IMPROVE Infectious disease Infectious diseases integration Interpretation membrane near-field communication nitrocellulose Nitrocellulose membrane Nucleocapsid proteins performed plasma extraction POC Point point of care point-of-care point-of-care (POC) diagnostics polyester Protein Quantitative Rapid reagent reagents required SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 nucleocapsid serological test Serological tests serology Smartphone Surveillance Viral whole blood sample [DOI] 10.1021/acssensors.1c01527 PMC 바로가기 [Article Type] Article
Functional convalescent plasma antibodies and pre-infusion titers shape the early severe COVID-19 immune response기능적 회복기 혈장 항체 및 주입 전 역가는 초기 중증 COVID-19 면역 반응을 형성합니다Article Published on 2021-11-252022-09-10 Journal: Nature Communications [Category] MERS, SARS, 유전자 메커니즘, 임상, 진단, 치료기술, 치료법, [키워드] administration Anti-nucleocapsid antibodies anti-nucleocapsid antibody Anti-spike anti-Spike IgG antibodies antibody Antibody titer binding convalescent plasma Evolution function functional heterogeneous nature heterogenous hospitalized COVID-19 patient hospitalized COVID-19 patients humoral humoral immune responses Humoral immunity Humoral response humoral responses immune immune response immune responses modulation nucleocapsid-specific antibody outcomes pandemic Patient plasma profile Receptor binding recipient SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 pandemic severe COVID-19 Severe infection Symptoms the SARS-CoV-2 therapy transfer Translational immunology Trial viral infection [DOI] 10.1038/s41467-021-27201-y PMC 바로가기 [Article Type] Article
High sensitivity-low cost detection of SARS-CoV-2 by two steps end point RT-PCR with agarose gel electrophoresis visualization아가 로스 겔 전기 영동 시각화를 사용한 2 단계 종점 RT-PCR에 의한 SARS-COV-2의 고감도 낮은 비용 감지Article Published on 2021-11-042022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 agarose gel electrophoresis Amplicon analytical sensitivity carried CDC coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 end point equipment gold gold standard Laboratory limit of detection limitation Microbiology Molecular biology nasopharyngeal sample Nasopharyngeal samples outbreak pandemic PCR reaction primer protocol reagent required respiratory ribonuclease RNase P RT-PCR RT-PCR assay RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleocapsid sensitivity severe acute respiratory syndrome Coronavirus specificity Viral Viral RNA Virology [DOI] 10.1038/s41598-021-00900-8 PMC 바로가기 [Article Type] Article