High sensitivity-low cost detection of SARS-CoV-2 by two steps end point RT-PCR with agarose gel electrophoresis visualization아가 로스 겔 전기 영동 시각화를 사용한 2 단계 종점 RT-PCR에 의한 SARS-COV-2의 고감도 낮은 비용 감지Article Published on 2021-11-042022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 agarose gel electrophoresis Amplicon analytical sensitivity carried CDC coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 end point equipment gold gold standard Laboratory limit of detection limitation Microbiology Molecular biology nasopharyngeal sample Nasopharyngeal samples outbreak pandemic PCR reaction primer protocol reagent required respiratory ribonuclease RNase P RT-PCR RT-PCR assay RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleocapsid sensitivity severe acute respiratory syndrome Coronavirus specificity Viral Viral RNA Virology [DOI] 10.1038/s41598-021-00900-8 PMC 바로가기 [Article Type] Article
Genomics-guided targeting of stress granule proteins G3BP1/2 to inhibit SARS-CoV-2 propagationSARS-CoV-2 전파를 억제하기 위한 스트레스 과립 단백질 G3BP1/2의 유전체학 유도 표적화Article Published on 2021-11-012022-09-11 Journal: International journal of biological macromolecules [Category] SARS, 유전자 메커니즘, 치료법, [키워드] Analysis candidate drug candidate drugs COVID-19 mitigation Decitabine docking drug drugs enrichment expression FOXA1 G3BP1 G3BP1/2 G3BP2 Gene Expression Gene set enrichment Genes GTPase Host host cells identify Imatinib inhibit inhibit SARS-CoV-2 Meta-analysis modulating molecular docking analysis much higher N protein nucleocapsid nucleocapsid protein Panel Propagation Protein Proteins regulatory element SARS-CoV-2 SARS-CoV-2 N protein SARS-CoV-2 nucleocapsid SIN3A SRF Stress stress granule SYK TGFBR2 the SARS-CoV-2 upstream YY1 [DOI] 10.1016/j.ijbiomac.2021.09.018 PMC 바로가기 [Article Type] Article
Longitudinal humoral antibody response to SARS-CoV-2 infection among healthcare workers in a New York City hospital뉴욕시 병원의 의료 종사자들 사이에서 SARS-CoV-2 감염에 대한 세로 체액성 항체 반응Article Published on 2021-10-262022-09-11 Journal: BMJ Open [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] addition age anti-S anti-S antibody antibodies antibody Antibody Response Asymptomatic clinical symptom Clinical symptoms completion conducted correlation COVID-19 COVID-19 symptom COVID-19 symptoms decay detect Diseases domain duration of symptom Dynamics ELISA evaluate faster Fever followed by full-length S Gastrointestinal symptom gastrointestinal symptoms HCW HCWs healthcare worker Healthcare workers hospital humoral humoral immune response humoral immune responses Humoral response immune responses immunology Infection Infectious diseases initial longitudinal longitudinal study Mount Sinai New York New York City outcome participant participated Phase 1 Phase 2 positive positive correlation Protein Quantitative reactive Receptor-binding domain S protein SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-COV-2 infection SARS-CoV-2 nucleocapsid SARS-CoV-2 PCR serology testing Significant South subject subsequent symptomatic System titre Viral was performed [DOI] 10.1136/bmjopen-2021-051045 PMC 바로가기 [Article Type] Article
SARS-CoV-2 exploits host DGAT and ADRP for efficient replicationArticle Published on 2021-10-262022-10-04 Journal: Cell Discovery [Category] 치료제, [키워드] acute respiratory syndrome adipocyte Cohort Complete coronavirus Course COVID-19 DGAT1 dominant dysregulation enzyme facilitate Factor Gene Expression Host inhibitor Mechanisms of disease metabolism metabolomics plasma Protein pulmonary inflammation reduce Replication respiratory tract infection SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 protein SARS-CoV-2 replication significantly suppress the disease Treatment triacylglycerol viral genome viral replication [DOI] 10.1038/s41421-021-00338-2 PMC 바로가기 [Article Type] Article
Cardiac Fibrosis Is a Risk Factor for Severe COVID-19심장 섬유증은 심각한 COVID-19의 위험 요소입니다Immunology Published on 2021-10-222022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Analysis biochemical analysis Blood cardiac cellular Clinical deterioration Clinical outcome Cohort Contact controls coronavirus coronavirus disease Coronavirus disease 2019 correlated strongly COVID-19 COVID-19 patients Deceased disease severity echocardiographic echocardiography elevated emergency department explain Factor fibrosis growth factor heart Increased Infection left lung Lungs marker matched controls mRNA mRNA level mRNA levels Myocardium NRP-1 Patient patients hospitalized performed predicted Prevalence pulmonary fibrosis quantification reported respiratory risk risk factor RNA SARS-CoV-2 SARS-CoV-2 nucleocapsid severe disease progression sIL1 TGF-β1 TGF-β1 transforming growth factor ventricular Viral viral entry with COVID-19 [DOI] 10.3389/fimmu.2021.740260 PMC 바로가기 [Article Type] Immunology
Infrequent Placental and Fetal Involvement in SARS-CoV-2 Infection: Pathology Data from a Large Medical CenterArticle Published on 2021-10-162022-10-29 Journal: Journal of Developmental Biology [Category] COVID-19, [키워드] accompanied analyzed association Autopsy center Clinical severity COVID-19 determine fetal fetal demise Frequency IHC immunohistochemistry Infection Inflammation Infrequent intervillositis Involvement ISH Mortality Neonate PCR performed Perinatal Placenta placental Placental infection positive positive COVID-19 Pregnancy protective role Pulmonary endothelium RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid SARS-CoV-2 PCR selected serology tested tissue tissue homogenates Transmission virus women [DOI] 10.3390/jdb9040045 PMC 바로가기 [Article Type] Article
Quantifying SARS-CoV-2 nucleocapsid antigen in oropharyngeal swabs using single molecule array technology단일 분자 어레이 기술을 사용하여 구인두 면봉에서 SARS-CoV-2 뉴클레오캡시드 항원 정량화Article Published on 2021-10-132022-09-10 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료법, [키워드] 95% CI ambition analyzed Antigen Biochemistry can be used clinical sensitivity clinically comparable correlated Culture cut-off demonstrated develop house lysis buffer Microbiology nucleocapsid antigen oropharyngeal swab oropharyngeal swabs Point positive real time real-time RT-PCR reference method routine clinical practice RT-PCR Sample SARS-CoV-2 SARS-CoV-2 infected patient SARS-CoV-2 infected patients SARS-CoV-2 nucleocapsid SARS-CoV-2 RNA SARS-CoV-2 virus sensitivity Simoa specificity Testing treated virus culture was used [DOI] 10.1038/s41598-021-99807-7 PMC 바로가기 [Article Type] Article
SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNASARS-COV-2 Nucleocapsid 단백질은 바이러스 게놈 RNA와 응축수를 형성한다Research Article Published on 2021-10-112022-09-10 Journal: PLoS Biology [Category] MERS, SARS, 유전자 메커니즘, 치료법, [키워드] acute respiratory syndrome coronavirus Alter asymmetric Base pairing cause coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 Cross-linking Deletion disrupt form genomic RNA global health Health host cells identify in vitro infected cells Infection inhibit Interaction interactions mammalian cells mass spectrometry mechanism membrane N protein N proteins nucleocapsid pandemic proliferation Protein respiratory RNA RNA genome SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 nucleocapsid severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 shape small molecule Small molecules substrate the N protein the SARS-CoV-2 the SARS-CoV-2 genome Viral viral genome viral genomic RNA viral particle Viral RNA virion while [DOI] 10.1371/journal.pbio.3001425 PMC 바로가기 [Article Type] Research Article
Prevalence of IgG antibodies induced by the SARS-COV-2 virus in asymptomatic adults in Nuevo Leon, Mexico멕시코 누에보레온의 무증상 성인에서 SARS-COV-2 바이러스에 의해 유도된 IgG 항체의 유병률Article Published on 2021-10-012022-09-11 Journal: Journal of medical virology [Category] MERS, SARS, 진단, 치료법, [키워드] 95% CI 95% confidence interval Adults age analyzed Asymptomatic asymptomatic individual Asymptomatic individuals carried coronavirus COVID-19 COVID-19 vaccine cross-sectional cross-sectional study Diagnosis distribution eligible Evidence evidence of Geographic distribution highest house IgG IgG antibodies IgG antibody Immunoglobulin Immunoglobulin G individual Latin America MOST no difference no differences nucleocapsid protein Prevalence preventive measure preventive measures previous diagnosis promotion Protein reported SARS-COV-2 infection SARS-CoV-2 nucleocapsid SARS-CoV2 screened Seroprevalence Sex Symptom virus was done Week [DOI] 10.1002/jmv.27131 PMC 바로가기 [Article Type] Article
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasmaCOVID-19 회복기 혈장에서 SARS-CoV-2 특이 IgG 검출을 위한 다중 코로나바이러스 항원 어레이 평가Article Published on 2021-10-012022-09-11 Journal: Journal of Immunological Methods [Category] MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] Antibody Response Antibody test Antibody titers Antigen Antigen array antigens comparable convalescent plasma convalescent plasma samples coronavirus COVID-19 COVID-19 pandemic cross-reactivity domain ELISA ELISAs Endemic evaluated Extracellular focus FRNT full-length HCoV HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HKU1 IgG IgG antibody Inc InDevR lack Live virus MERS MERS-CoV Mitigation Neutralizing activity neutralizing antibody neutralizing antibody titers NL63 nucleocapsid nucleocapsid IgG OC43 other coronavirus other coronaviruses plasma samples platform positive result positive sample proximal question reactivity Receptor binding domain reduction remained reported Sample SARS-CoV SARS-CoV spike SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-CoV-2 IgG SARS-CoV-2 IgG antibody SARS-CoV-2 nucleocapsid Sensitivity and specificity significantly higher specificity spike Spike antigen the antibody response the SARS-CoV-2 while [DOI] 10.1016/j.jim.2021.113104 PMC 바로가기 [Article Type] Article