Dilution – Page 13 – Communicable Diseases Research

In vitro virucidal activity of Echinaforce®, an Echinacea purpurea preparation, against coronaviruses, including common cold coronavirus 229E and SARS-CoV-2

Research

Published on 2020-09-092022-10-28
Journal: Virology Journal [Category] COVID-19, SARS,

Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA

Editor's Choice

Published on 2020-09-052022-10-29
Journal: Journal of Medical Microbiology [Category] COVID-19, SARS,

[키워드] alleviate average bypassing CDC clinical sample Combination conducted COVID-19 detect diagnostic diagnostics Dilution E-gene enzyme evaluated FAM FDA implementation intervals isothermal amplification Laboratory medium Nasopharyngeal swab nasopharyngeal swabs nucleocapsid primer required resource Result reverse transcription RNA extraction kit RNA purification RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA SARS-CoV-2 virus screened Sensitivity and specificity specimen TCID the SARS-CoV-2 thermocycler thermocyclers Transport tube universal transport media UTM validation virus detection
[DOI] 10.1099/jmm.0.001238 PMC 바로가기 [Article Type] Editor's Choice

Evaluation of Four Commercial Kits for SARS-CoV-2 Real-Time Reverse-Transcription Polymerase Chain Reaction Approved by Emergency-Use-Authorization in Korea

Medicine

Published on 2020-08-132022-10-31
Journal: Frontiers in Medicine [Category] COVID-19,

[키워드] 2019-nCoV approved association chain clinical sample collected consecutive sample Coronavirus infectious disease 2019 COVID-19 COVID-19 patient cross-reactivity detection detection rate Diagnosis Dilution effective emergency-use-authorization Epidemics EUA evaluated examined expressed Follow-up indicated limit of detection log Nasopharyngeal swab nCoV negative control NPs parameters PCR performance polymerase chain reaction positive Quality control reaction real time Real-time reverse-transcription PCR reverse transcription RNA rRT-PCR SARS-CoV SARS-CoV-2 sensitivity analysis severe acute respiratory syndrome coronavirus 2 sputum Stool transcribed Treatment Urine Vaccine Viral concentration was determined
[DOI] 10.3389/fmed.2020.00521 PMC 바로가기 [Article Type] Medicine

Pooling of coronavirus tests under unknown prevalence

Short Paper

Published on 2020-08-062022-11-01
Journal: Epidemiology and Infection [Category] COVID-19, MERS,

[키워드] absence coronavirus coronavirus disease COVID-19 diagnostic testing Dilution Effects Epidemiology False positive FIVE Laboratory tests Mathematical modelling Novel coronavirus pandemic pool pooling Prevalence probe protocol reduce
[DOI] 10.1017/S0950268820001752 PMC 바로가기 [Article Type] Short Paper

Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates

Original Article

Published on 2020-07-282022-11-01
Journal: The New England Journal of Medicine [Category] COVID-19, MERS, SARS,

[키워드] acute respiratory syndrome Analysis antibody Antigen BAL BAL fluid CD4 T-cell CD8 T-cell responses changes in coronavirus coronavirus disease detectable Dilution dose eight evaluate geometric mean titer groups histopathological Inflammation inhibitory lower airway lower airways lung mRNA-1273 mRNA-1273 vaccine nasal swab Neutralizing activity pathologic performed polymerase chain Prevent primate quantification Replication response Result robust SARS-CoV-2 serum Seven specimen specimens Spike protein T-cell T-cell Response Th2 type 1 helper undetectable Vaccine vaccine group viral genome viral replication were assessed
[DOI] 10.1056/NEJMoa2024671 PMC 바로가기 [Article Type] Original Article

A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2

Article

Published on 2020-07-072022-10-31
Journal: Heliyon [Category] COVID-19,

[키워드] Accuracy Analysis Analyzer automated clinical sample clinical samples Cohort COVID COVID qPCR analyzer COVID-19 COVID-19 detection assay COVID-19 pandemic detect develop diagnosis of SARS-CoV-2 diagnostics Dilution evaluated experiment Genomics graphical user interface Graphical user interface (GUI) human RNA in vitro infected patient infected with SARS-CoV-2 Infectious disease Internal control less limit Microbial genomics Molecular biology multiplex Patient performed qPCR qRT-PCR Real-time PCR Real-time RT- PCR real-time RT-PCR reference gene RNase P SARS-CoV-2 SARS-CoV-2 testing TaqMan tested the SARS-CoV-2 viral transcripts Virology
[DOI] 10.1016/j.heliyon.2020.e04405 PMC 바로가기 [Article Type] Article

Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay

Article

Published on 2020-07-072022-10-06
Journal: Journal of virological methods [Category] MERS, SARS, 진단,

[키워드] 2019-nCoV acute respiratory syndrome addition assays baseline CDC China clinical samples conducted coronavirus coronavirus 2 COVID-19 Critical detect detection rate Detection sensitivity determine develop Diagnosis diagnostic Dilution disease spread DNA IMPROVE Improved Japan N1 and N2 Nasopharyngeal swab nasopharyngeal swabs Outbreaks patients polymerase chain positive positive control primer probe probes provided Quencher required respiratory Result RT-PCR RT-PCR assay SARS-CoV-2 sensitivity sequence specificity Spread tested the SARS-CoV-2 threshold USA virus were used Wuhan
[DOI] 10.1016/j.jviromet.2020.113926 PMC 바로가기 [Article Type] Article

Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples

임상 샘플에서 직접 SARS-CoV-2 게놈의 대규모 병렬 시퀀싱을 위한 여러 접근 방식

Research

Published on 2020-06-302022-08-13
Journal: Genome Medicine [Category] SARS, 유전자 메커니즘,

[키워드] (coronavirus disease 2019 Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amplicon amplicon method Amplicon sequencing analyses Analysis applied approach approaches best capture capture method caused Characteristics clinical sample clinical samples Complete coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cultured isolate demonstrated diagnostics Dilution Efficiency eight Emerging infectious diseases Epidemic Epidemiology Evolution examined Factor full-length SARS-CoV-2 genetic information Genome Genome sequencing genomes Genomic surveillance Guidance Hybrid capture inter- and intra-individual variations inter-individual inter-individual and intra-individual variations intra-host dynamics intra-individual variation iSNV knowledge Metatranscriptomic sequencing Multiple Multiplex PCR novel multiplex PCR amplicon offer PCR amplicon Quasispecies reported respiratory Result SARS-CoV-2 SARS-CoV-2 genome scarcity sensitivity Sequencing severe acute respiratory syndrome Coronavirus Therapeutics ultra-high-throughput metatranscriptomic variations Viral Viral load viral loads viral read Viral transmission virus Virus evolution Virus-host interaction Virus-host interactions viruses whole-genome sequencing
[DOI] 10.1186/s13073-020-00751-4 PMC 바로가기 [Article Type] Research

Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs

Article

Published on 2020-06-202022-10-29
Journal: International Journal of Molecular Sciences [Category] COVID-19, SARS,

Evaluating the efficacy and safety of human anti-SARS-CoV-2 convalescent plasma in severely ill adults with COVID-19: A structured summary of a study protocol for a randomized controlled trial

Letter

Published on 2020-06-082022-10-29
Journal: Trials [Category] COVID-19, SARS,

[키워드] activity adverse event allergic reaction anti-SARS-CoV-2 anti-SARS-CoV-2 antibody Anti-SARS-CoV-2 plasma Anti-viral blinded Blood blood product center Chest radiography Clinical improvement Clinical outcome clinical status clinical trial clinician collected Columbia University control arm convalescent plasma coronavirus COVID-19 COVID-19 symptom criteria cumulative Date defined Dilution discharge dissemination Donor ECMO Efficacy Efficacy and safety element evaluate Evaluating Evidence extracorporeal membrane oxygenation hazard hazard ratio HBV HCV HIV hospital Hospitalized hospitalized patient IgA deficiency immune incidence include Infection Influenza Invasive mechanical ventilation mechanical ventilation multi-organ failure nasopharyngeal Nasopharyngeal swab New York Non-invasive number objective occur oropharyngeal swab outcome oxygen saturation participant Participants patients hospitalized PCR test PCR-negative per-protocol analysis peripheral capillary plasma platform post-hoc analysis Pregnancy primary endpoint protocol randomization Randomized Randomized controlled trial randomized, controlled trial randomly permuted block receive Registered Remdesivir requiring high-flow oxygen requiring supplemental oxygen Research Respiratory failure retrospective Ritonavir safety endpoint Sample size SARS-CoV-2 SARS-CoV-2 antibody SARS-COV-2 infection screened Serious Adverse Event seven-point ordinal scale severe COVID-19 severe SARS-CoV-2 severely ill adult single-center spike trimer SpO2 status study participant study period Study protocol supplemental oxygen tested therapy transfused Treatment treatment for COVID-19 Trial undergo USA Version Volunteer Wald test website with COVID-19 World Health Organization ZIKV
[DOI] 10.1186/s13063-020-04422-y PMC 바로가기 [Article Type] Letter