T Cells Plead for Rejuvenation and Amplification; With the Brain’s Neurotransmitters and Neuropeptides We Can Make It Happen회춘과 증폭을 위한 T 세포 주장; 뇌의 신경전달물질과 신경펩티드로 우리는 그것을 실현할 수 있습니다Review Published on 2021-03-222022-09-11 Journal: Frontiers in Immunology [Category] SARS, 유전자 메커니즘, [키워드] activate activated acute SARS-CoV-2 infection Adoptive Cell Therapy aging binding Brain Can Cancer Cell cell surface chemotactic chronic infections clinical trials CNS cytokine cytokine secretion damage decrease Depression diagnostic Dopamine Effect Effects Efficacy Ex vivo expressed extravasation Gene Expression Glutamate GnRH high risk Immunotherapy IMPROVE induce Infection infections Inflammation Injury integrin integrin-mediated killing limit microorganism microorganisms Migration Mortality natural Nerve-Driven Immunity nervous Neuropeptide Neuropeptide Y Neuropeptides neurotransmitter Neurotransmitters organization organizations other diseases other treatments overcome PD-1 proliferation protocols receptor Rejuvenation repeated SARS-COV-2 infection senescent Somatostatin Stress suggested System T cell T cells tested the cell therapeutic tissue repair trauma [DOI] 10.3389/fimmu.2021.617658 PMC 바로가기 [Article Type] Review
Ex vivo assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells비강 상피 세포의 SARS-CoV-2 감염 예방에 있어 스핑고지질을 표적으로 하는 약물의 효능을 평가하기 위한 생체 외 분석Article Published on 2021-03-192022-08-31 Journal: STAR protocols [Category] MERS, SARS, 신약개발, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 amitriptyline antidepressant approved assays Cell isolation Cell-based assays. Complete Coronavirus-2 COVID-19 drug drugs targeting Efficacy epithelial cell epithelial cells evaluate Ex vivo Infection infection level infection levels inhibitor nasal epithelial cell offer Particle presenting protocol quantified Replication respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sphingolipid Spike protein stomatitis the SARS-CoV-2 vesicular stomatitis vesicular stomatitis virus Volunteer were infected [DOI] 10.1016/j.xpro.2021.100356 PMC 바로가기 [Article Type] Article
Antibody-guided in vivo imaging of Aspergillus fumigatus lung infections during antifungal azole treatmentArticle Published on 2021-03-172022-10-04 Journal: Nature Communications [Category] 바이오마커, [키워드] A. fumigatus approach Aspergillus Aspergillus fumigatus can be used caused Complete Critical Diagnosis diagnostic procedure drug early diagnosis Efficacy Ex vivo fluorescence microscopy fungal Fungal infection humans Immunocompromised in vivo Infection Intervention Invasion Invasive pulmonary aspergillosis IPA life-threatening lung Lung disease Lung infection Medical imaging molecular MONITOR monoclonal antibody Non-invasive pathogen patient survival Prevent reveal the disease therapeutic options therapy Treatment [DOI] 10.1038/s41467-021-21965-z PMC 바로가기 [Article Type] Article
Integrated cytokine and metabolite analysis reveals immunometabolic reprogramming in COVID-19 patients with therapeutic implicationsArticle Published on 2021-03-122022-10-04 Journal: Nature Communications [Category] SARS, 바이오마커, 치료기술, 치료법, [키워드] Affect alteration arginine association change chemokine Correlation analysis COVID-19 COVID-19 patient COVID-19 patients CRS cytokine cytokine release Cytokines Diseases Ex vivo healthy controls Host hyperinflammation IL-1α IL-1β IL-6 immune response implication Inflammatory response Injury M-CSF metabolic pathway metabolism metabolite metabolite analysis metabolomic metabolomics Mild modulate mononuclear cell outcome Peripheral blood Prognostic markers proinflammatory Proinflammatory cytokine purine Regulatory reveal rhesus macaque SARS-CoV-2 SARS-COV-2 infection serum sample severe COVID-19 patients stratified syndrome therapeutic therapeutic target [DOI] 10.1038/s41467-021-21907-9 PMC 바로가기 [Article Type] Article
In the eye of the storm: SARS‐CoV‐2 infection and replication at the ocular surface?Human Clinical Article Published on 2021-03-122022-10-28 Journal: Stem cells translational medicine [Category] COVID-19, SARS, [키워드] ACE2 acute respiratory syndrome Analysis carried Cell clinical presentation Conjunctiva conjunctival Cornea Corneal coronavirus coronavirus disease COVID‐19 COVID‐19 Culture Efficiency evaluate Ex vivo Factor feature highlight immune response Infection limbus ocular Ocular surface organ organ and animal models organ systems Organoid pandemic performed prophylactic treatment recent Replication SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‐CoV‐2 Schematic scRNA‐seq shown Single Cell Spread tears therapy TMPRSS2 translated [DOI] 10.1002/sctm.20-0543 PMC 바로가기 [Article Type] Human Clinical Article
Deciphering the state of immune silence in fatal COVID-19 patientsArticle Published on 2021-03-052022-10-04 Journal: Nature Communications [Category] SARS, 바이오마커, 신약개발, 진단, 치료기술, 치료법, 치료제, [키워드] Activation Analysis BAL Blood blood sample CD4-positive T cells Cell cell type characterized Clinical outcome COVID-19 COVID-19 patient Critical CXCR6 disease dysregulation effector memory Evolution Ex vivo expression fatal COVID-19 feature Features functional hallmark highlighting immune immune mechanism immune signature Immune suppression immunological impairment lung lymphoid lymphoid cell Monocytes myeloid cells naïve pandemic pathology Patient Prognosis Regulatory SARS-CoV-2 severe COVID-19 severe patients severity Single-cell RNA sequencing single-cell RNA-seq analysis T cell subset tissue tissue site to define unique viral infection [DOI] 10.1038/s41467-021-21702-6 PMC 바로가기 [Article Type] Article
SARS-CoV-2 mutations in MHC-I-restricted epitopes evade CD8 + T cell responsesMHC-I 제한 에피토프의 SARS-CoV-2 돌연변이는 CD8 + T 세포 반응을 회피합니다Research Articles Published on 2021-03-042022-09-10 Journal: Science Immunology [Category] Fulltext, SARS, 변종, 진단, [키워드] abrogated binding CD8 Cell COVID-19 patient COVID-19 patients COVID-19 severity CTL cytotoxic activity deep sequencing epitope Epitopes evade Ex vivo exhibited highlight IFN-γ Immunity implicated in vitro isolates MHC-I MHC-I epitope MHC-I epitopes mutant nonsynonymous mutation Nonsynonymous mutations peptide peptides Point mutation point mutations proliferation response RNA sequencing SARS-CoV-2 SARS-CoV-2 mutation SARS-CoV-2 mutations SARS-CoV-2 virus Sequencing Surveillance T cell T cell epitope T cell epitopes T cells tetramer transcriptional response Viral virus [DOI] 10.1126/sciimmunol.abg6461 PMC 바로가기 [Article Type] Research Articles
Inactivation and Elimination of SARS-CoV-2 in Biosamples Using Simple Fixatives and UltrafiltrationProtocol Published on 2021-02-272022-10-30 Journal: Methods and Protocols [Category] COVID-19, [키워드] accelerated affected Analysis biocontainment biological sample BSL-3 BSL2 cause Cell Cell culture Complete conducted COVID-19 cut-off downstream analysis effective Effectiveness eliminating Elimination Ethanol Ex vivo fixative formalin handling Health host cell in vitro in vivo model inactivated inactivation individual Infection infection risk kDa Laboratory limit lung tissue lysates media membrane membrane filter molecular weight neutral buffered formalin pandemic paraformaldehyde pathogenesis of SARS-CoV-2 pathogenicity pathological analysis plaque forming units reduce Research respiratory Safe SARS-CoV-2 SARS-CoV-2 infected cell significantly simple standard procedure subsequent tissue Treatment Ultrafiltration undetectable viral inoculum virus [DOI] 10.3390/mps4010018 PMC 바로가기 [Article Type] Protocol
Inflammation and Antiviral Immune Response Associated With Severe Progression of COVID-19COVID-19의 심각한 진행과 관련된 염증 및 항바이러스 면역 반응Clinical Trial Published on 2021-02-182022-08-13 Journal: Frontiers in Immunology [Category] MERS, 바이오마커, 신종인플루엔자, 유전자 메커니즘, 임상, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 applied coronavirus coronavirus disease coronavirus disease-2019 COVID-19 COVID-19 severity Disease progression disease severity downregulation Ex vivo Functional enrichment analysis H1N1 H1N1 virus healthy controls hemagglutinin host immune system hypercytokinemia IFNs immune cells immune response immune signature impair impaired function include indicate indicated Infection Inflammation Inflammatory Inflammatory response Influenza influenza A interferons LAG3 microarray analysis mild COVID-19 patient MMP9 mononuclear cell mononuclear cells myeloid cell myeloid cells Neutrophils novel respiratory disease overactivation overactive patients patients with influenza PBMC PBMCs Peripheral blood Peripheral blood mononuclear cells potential mechanism potential mechanisms progression promote proportion Protein regulate Regulation respiratory Respiratory disease respond response SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severe and mild COVID-19 patients severe COVID-19 severe SARS severe SARS-CoV-2 severity Signaling SKAP1 spike Spike protein Stimulation suggested T cell T cells T cells TLR6 Transcriptome Type I IFN type I IFNs type I interferon type I interferons unique upregulation whole genome transcriptome with COVID-19 [DOI] 10.3389/fimmu.2021.631226 PMC 바로가기 [Article Type] Clinical Trial
Carboxypeptidase B blocks ex vivo activation of the anaphylatoxin-neutrophil extracellular trap axis in neutrophils from COVID-19 patientsResearch Published on 2021-02-082022-11-01 Journal: Critical Care [Category] COVID-19, MERS, [키워드] Activation anaphylatoxin antibodies Bilirubin block carboxypeptidase Carboxypeptidase B cascade CCK-8 assay Cell cell-free DNA Coagulation Coagulopathy complement component contribute COVID-19 COVID-19 patient COVID-19 patients CPB2 Critically ill patient cytotoxicity Degradation degrade demonstrated Disease progression endothelial enzyme evaluated Ex vivo Extracellular help homolog Immunofluorescent staining immunothrombosis inactive Intervention lactate dehydrogenase myeloperoxidase NET NETs neutrophil Neutrophil extracellular trap pathological role plasma prevalent Prevent proinflammatory mediators recruited Regulation Result risk of death thrombosis thrombotic event total bilirubin Vascular vascular endothelial cell with COVID-19 [DOI] 10.1186/s13054-021-03482-z PMC 바로가기 [Article Type] Research