A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human CoronavirusesArticle Published on 2016-11-232022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, [키워드] Acute respiratory infection characteristic clinical clinical samples coronavirus demonstrated detection Detection sensitivity Diagnosis distribution fluorescent HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 hospitalized patient Human human coronaviruses IMPROVE Infection management melting Melting curve melting curve analysis melting temperature (Tm) multiplex quantitative RT-PCR (RT-qPCR) multiplex RT-qPCR primer Rapid respiratory virus RT-qPCR Sequencing Simultaneous SYBR Green I SYTO 9 temperature Treatment was used [DOI] 10.3390/ijms17111880 PMC 바로가기 [Article Type] Article
HTCC: Broad Range Inhibitor of Coronavirus EntryResearch Article Published on 2016-06-012022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] acute infection block chloride common cold coronavirus drug effective inhibitor HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 highly pathogenic Hospitalization human coronavirus human coronaviruses humans Immunocompromised patient Infants Infection inhibit inhibitor Interaction MERS coronaviruses Polymer Range receptor respiratory infection severe disease therapy virus [DOI] 10.1371/journal.pone.0156552 PMC 바로가기 [Article Type] Research Article
Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in ChinaResearch Paper Published on 2016-04-152022-10-28 Journal: Science China. Life sciences [Category] Coronavirus, [키워드] Analysis antigenic antigenicity Antisera Beijing canine Canine coronavirus canine respiratory coronavirus China cloned conserved coronavirus Coronavirus infection cross-react cross-reactivity E. coli Escherichia coli expressed expression Final group HCoV-229E HKU1 human coronaviruses indicated mice N gene NL63 nucleocapsid protein OC43 polyacrylamide gel purified raised Respiratory Coronavirus SDS-PAGE sequenced size exclusion chromatography sodium dodecyl sulfate Swab virus were expressed [DOI] 10.1007/s11427-016-5038-1 PMC 바로가기 [Article Type] Research Paper
Molecular characterization of human coronaviruses and their circulation dynamics in Kenya, 2009–2012Research Published on 2016-02-012022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] Acute respiratory infection all age group amplification Analysis analyzed cells characterized children circulation diagnosed distribution eight Evidence FIVE HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 Hospitalization Human human coronavirus human coronaviruses identity Immunity individual Infection information inoculated isolate Kenya MERS-CoV molecular molecular characterization monolayer morbidity nasopharyngeal specimen polymerase positive Prevalence Primers real time Respiratory illness responsible Result RT-PCR sequence homology Sequencing shown specimen specimens strain Strains Variation virus [DOI] 10.1186/s12985-016-0474-x PMC 바로가기 [Article Type] Research
Glucose-6-Phosphate Dehydrogenase Enhances Antiviral Response through Downregulation of NADPH Sensor HSCARG and Upregulation of NF-κB SignalingArticle Published on 2015-12-172022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] activity Alpha Analysis Antiviral antiviral response Cell cells coronavirus Degradation dehydrogenase DNA binding downregulated enterovirus examined Exogenous expression G6PD glucose-6-phosphate dehydrogenase GTPase HCoV-229E HSCARG human coronavirus 229E IDH1 increased expression Infection inhibited IκB mechanism MX1 NADPH Necrosis NF-κB NF-κB signaling nicotinamide adenine dinucleotide overexpression phosphate promoter Protein reduced response resulting sensor siRNA suppressed susceptibility susceptible TNF- α upregulated viral gene expression viral infection virus infection [DOI] 10.3390/v7122966 PMC 바로가기 [Article Type] Article
The Nucleocapsid Protein of Human Coronavirus NL63Research Article Published on 2015-02-202022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Analysis binding C-terminal domain Cell cell cycle progression cellular processes Characteristics Coronaviral coronavirus described disease expressing feature Genetic genomes HCoV-229E HCoV-NL63 homologous Human human coronavirus human coronavirus 229E infected cells involved N-terminal domain NL63 nucleic acid nucleocapsid protein nucleus oligomerization other coronaviruses pathogen Protein respiratory tract responsible reveal SARS-CoV severity subcellular localization the N protein transfected cells unique viral replication [DOI] 10.1371/journal.pone.0117833 PMC 바로가기 [Article Type] Research Article
Human Coronaviruses Associated with Upper Respiratory Tract Infections in Three Rural Areas of GhanaResearch Article Published on 2014-07-312022-10-28 Journal: PLoS ONE [Category] Coronavirus, MERS, [키워드] Area ARI asymptomatic subject case control study chain children conducted control subjects controls determine GenBank HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 Human human coronavirus Infection infections MERS-CoV Middle East morbidity and mortality no difference occurred Older positive reaction recruited respiratory respiratory syndrome coronavirus respiratory tract infection Result sequence subject Swab tested tract upper upper respiratory tract virus detection viruses [DOI] 10.1371/journal.pone.0099782 PMC 바로가기 [Article Type] Research Article
Human coronavirus NL63 replication is cyclophilin A-dependent and inhibited by non-immunosuppressive cyclosporine A-derivatives including AlisporivirArticle Published on 2014-02-222022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] alisporivir ALV, Alisporivir Caco-2 cell Cell culture cellular Concentration coronavirus Coronavirus replication CoV CoV infection CoV replication CsA/CsD, cyclosporine A/D cyclophilin cyclophilin A Cyclosporine/FK506-non-immunosuppressive derivatives CypA/B, cyclophilin A/B derivative EC50, 50% effective inhibitory concentration effective drug FK506 FKBP FKBP, FK506-binding protein growth HCoV-229E HCoV-NL63 Host Human human coronavirus Infection inhibit inhibited Inhibition of viral replication inhibitors magnitude New NIM811 NL63 PPIase, peptidyl prolyl cis/trans isomerase Prevent qPCR analysis Replication required SARS-CoV shown therapeutic approaches therapy virus replication Weber [DOI] 10.1016/j.virusres.2014.02.010 PMC 바로가기 [Article Type] Article
First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhoodResearch Article Published on 2013-09-162022-10-28 Journal: BMC Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome Adult Adults age anti-S IgG anti-S IgM antibody applied Asymptomatic Beijing blood sample Cell childhood children detect detectable detected Evidence General population HCoV-229E healthy human coronavirus IgM immunofluorescence assay (IFA Indirect immunofluorescence assay Infection majority occurred Prevalence proportion Protein recombinant S protein respiratory tract sample Result Seroconversion serum serum sample Spike protein tested virus were expressed [DOI] 10.1186/1471-2334-13-433 PMC 바로가기 [Article Type] Research Article
Identification and Characterization of a Novel Alpaca Respiratory Coronavirus Most Closely Related to the Human Coronavirus 229EArticle Published on 2012-12-122022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] 229E acute respiratory disease alpaca anthroponosis appear characterization coronavirus demonstrated FIVE Frame Genome genomic sequencing HCoV-229E Human human coronavirus humans identification identity isolate molecular evolution MOST novel Novel coronavirus Nucleocapsid gene nucleotide occurred respiratory reverse zoonosis spike gene sequence UTR virus virus transmission zoonosis [DOI] 10.3390/v4123689 PMC 바로가기 [Article Type] Article