Evolutionary and structural analysis elucidates mutations on SARS-CoV2 spike protein with altered human ACE2 binding affinity인간 ACE2 결합 친화도가 변경된 SARS-CoV2 스파이크 단백질의 돌연변이를 설명하는 진화적 및 구조적 분석Article Published on 2021-01-292022-09-11 Journal: Biochemical and Biophysical Research Communication [Category] 변종, [키워드] ACE2 ACE2 binding affinity Alter Analysis analyzed binding binding affinity binding energy binding free energy docking Efficacy ENhance evaluate facilitate FIVE free energy Free energy calculations G476S glycoprotein host cell human ACE2 hydrogen Hydrogen bond identify Mutation pandemic Population Population variants Positive selection Proteomic analysis RBD RBD variant RBD variants RBD-ACE2 Receptor-binding domain Recognition reduced residue reveal SARS-CoV2 SARS-CoV2 genome Selection Selection bias spike spike glycoprotein Spike protein spike variant Structural analysis the RBD the receptor-binding domain V367F V483A variant variations while Wuhan [DOI] 10.1016/j.bbrc.2021.01.035 PMC 바로가기 [Article Type] Article
Metabolic reprogramming and epigenetic changes of vital organs in SARS-CoV-2–induced systemic toxicitySARS-CoV-2에 의한 전신 독성에서 중요한 기관의 대사 재프로그래밍 및 후성 유전적 변화Research Article Published on 2021-01-252022-09-12 Journal: JCI Insight [Category] MERS, 진단, [키워드] administration Affect animal animals atrophy Biomarker Biomarkers change changes in complications of COVID-19 contribute COVID-19 COVID-19 pathogenesis COVID-19 phenotypes demonstrated develop DNA methylation edema electron microscopy Epigenetic epigenetics expressing expression Extrapulmonary failure failure to thrive followed by heart rate human ACE2 immune response gene immune response genes induce Intermediary metabolism internal organ internal organs lethality lymphopenia manifestation Manifestations metabolic metabolism metabolite metabolomic profiling Microscopy morbidity mortality rate much higher Multiorgan multiple organ multiple organs multiple tissue multiple tissues murine model Myocardial neutrophilia organ oxidative phosphorylation Pathogenesis pathogenic performed Peripheral blood phenotype SARS-CoV-2 significantly lower TCA cycle Toxicity transgene tricarboxylic acid weight loss [DOI] 10.1172/jci.insight.145027 PMC 바로가기 [Article Type] Research Article
Development of Plant-Produced Recombinant ACE2-Fc Fusion Protein as a Potential Therapeutic Agent Against SARS-CoV-2Plant Science Published on 2021-01-072022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] ACE2 ACE2-Fc ACE2-Fc fusion protein acute respiratory syndrome Against agent angiotensin-converting enzyme 2 Anti-SARS-CoV-2 Activity approved benthamiana binding cell entry receptor clinical trials coronavirus coronavirus disease COVID-19 development Efficacy exhibited expressed Fc region fused fusion fusion protein global public health host cell human ACE2 human IgG1 in vitro inhibit SARS-CoV-2 MERS-CoV molecular farming neutralization Nicotiana benthamiana plant-produced recombinant protein Potential produced RBD Receptor binding domain recombinant recombinant fusion protein SARS-CoV SARS-CoV-2 SARS-COV-2 infection suppressed therapeutic therapeutic drug Treatment Vaccine Vero Cell viral infection [DOI] 10.3389/fpls.2020.604663 PMC 바로가기 [Article Type] Plant Science
Elucidating Interactions Between SARS-CoV-2 Trimeric Spike Protein and ACE2 Using Homology Modeling and Molecular Dynamics SimulationsChemistry Published on 2021-01-052022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 Alanine Area binding build cause confirmed case coronavirus disease COVID-19 crystal structure death develop drug Dynamics enzyme facilitate FIVE full-length spike protein homology homology modeling host cell host cell receptor host cells human ACE2 in silico in viral Infection interacting residue Interaction Mechanics membrane fusion modeling Molecular dynamics simulation molecular dynamics simulations monomer Mutant SARS-CoV-2 outer membrane Prevent Protein RBD Receptor binding domain receptor protein reported residue respiratory responsible SARS-CoV-2 SARS-COV-2 infection Simulation spike Spike protein ssRNA surface the SARS-CoV-2 the spike protein treat triggering trimer trimeric Trimeric spike protein understanding Vaccine virion was used wild-type SARS-CoV-2 [DOI] 10.3389/fchem.2020.622632 PMC 바로가기 [Article Type] Chemistry
Cross-species recognition of SARS-CoV-2 to bat ACE2ACE2에 대한 SARS-CoV-2의 종간 인식530 Published on 2021-01-052022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] 변종, [키워드] Rhinolophus macrotis ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 attenuate bACE2 bat bat ACE2 bats benefit bind binding binding domain caused complex conserved coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Evidence expressing global health hACE2 Health host cell host cells human ACE2 human ACE2 receptor infect Infection Interaction less molecular basis mutant pandemic pseudotyped pseudotyped SARS-CoV-2 virus RBD RBD binding receptor Receptor binding domain residue residues respiratory SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 receptor SARS-CoV-2 spike protein receptor binding domain SARS-CoV-2 virus severe acute respiratory syndrome Coronavirus Significance Spike protein the SARS-CoV-2 Variation variations virus virus infection was determined Y41H [DOI] 10.1073/pnas.2020216118 PMC 바로가기 [Article Type] 530
Functional prediction and frequency of coding variants in human ACE2 at binding sites with SARS-CoV-2 spike protein on different populations다양한 집단에서 SARS-CoV-2 스파이크 단백질이 있는 결합 부위에서 인간 ACE2의 코딩 변이체의 기능적 예측 및 빈도Comment Published on 2021-01-012022-09-11 Journal: Journal of medical virology [Category] SARS, 변종, [키워드] binding site coding variant Frequency human ACE2 Population SARS-CoV-2 spike protein [DOI] 10.1002/jmv.26126 PMC 바로가기 [Article Type] Comment
Evolutionary and structural analysis elucidates mutations on SARS-CoV2 spike protein with altered human ACE2 binding affinity인간 ACE2 결합 친화도가 변경된 SARS-CoV2 스파이크 단백질의 돌연변이를 설명하는 진화적 및 구조적 분석Article Published on 2021-01-012022-09-11 Journal: Biochemical and Biophysical Research Communication [Category] SARS, 변종, [키워드] ACE2 ACE2 binding affinity Alter Analysis analyzed binding binding affinity binding energy binding free energy docking Efficacy ENhance evaluate facilitate FIVE free energy Free energy calculations G476S glycoprotein host cell human ACE2 hydrogen Hydrogen bond identify Mutation pandemic Population Population variants Positive selection Proteomic analysis RBD RBD variant RBD variants RBD-ACE2 Receptor-binding domain Recognition reduced residue reveal SARS-CoV2 SARS-CoV2 genome Selection Selection bias spike spike glycoprotein Spike protein spike variant Structural analysis the RBD the receptor-binding domain V367F V483A variant variations while Wuhan [DOI] 10.1016/j.bbrc.2020.11.075 PMC 바로가기 [Article Type] Article
High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme고친화성 나노바디는 SARS-CoV-2 스파이크 수용체 결합 도메인과 인간 안지오텐신 전환 효소의 상호작용을 차단합니다Article Published on 2020-12-222022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 변종, 진단, [키워드] ACE2 angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 antibody fragment approved Biochemistry block COVID-19 pandemic derivative diagnostic Drug development effective enzyme expressing form HEK293 cell HEK293 cells high affinity human ACE2 Infection inhalation Interaction lead lentivirus Medical research nanobodies nanobody nebulization potency Proteins pseudotyped Receptor binding domain responsible SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein receptor binding domain Spike protein the SARS-CoV-2 the spike protein therapeutic Treatment variant virus [DOI] 10.1038/s41598-020-79036-0 PMC 바로가기 [Article Type] Article
Functional Complexes of Angiotensin-Converting Enzyme 2 and Renin-Angiotensin System Receptors: Expression in Adult but Not Fetal Lung Tissue안지오텐신 전환 효소 2 및 레닌-안지오텐신 시스템 수용체의 기능적 복합체: 태아 폐 조직이 아닌 성인에서의 발현Article Published on 2020-12-162022-09-12 Journal: International Journal of Molecular Sciences [Category] MERS, SARS, 진단, [키워드] ACE2 activate acute respiratory syndrome alteration analogy angiotensin Angiotensin II angiotensin receptor Angiotensin-converting enzyme angiotensin-converting enzyme 2 assays binding CAMP CD26 Cell cell entry chemokine Chemokine receptor cleave complexes component Coronaviruses COVID-19 CXCL12 CXCR4 dipeptidyl peptidase Dipeptidyl peptidase-4 downregulation energy transfer enzyme expression fetal G-protein highlight Human human ACE2 Human immunodeficiency virus immunodeficiency virus infect interact Interaction Ligand lung lung tissue Lungs macromolecular Mas receptor MAS1 mechanism membrane mitogen-activated protein kinase organs performed potential therapeutic targets Protein protein kinase RAS receptor receptors Renin renin-angiotensin system respiratory SARS-COV-2 infection SARS-CoV-2 receptor SDF-1 Severe acute respiratory syndrome Signal transduction Signaling System target cell target cells upregulation while [DOI] 10.3390/ijms21249602 PMC 바로가기 [Article Type] Article
Temperature dependence of the SARS-CoV-2 affinity to human ACE2 determines COVID-19 progression and clinical outcomeResearch Article Published on 2020-12-162022-10-31 Journal: Computational and Structural Biotechnology Journal [Category] COVID-19, [키워드] Abstract ACE2 affinity Analysis bind binding binding affinity body temperature Caco-2 cells cause Clinical data Clinical outcome Coronaviruses COVID-19 COVID-19 patients COVID-19 progression demonstrated determine early stage Efficiency enzyme II evidenced explained febrile Fever homolog human ACE2 human ACE2 receptor human cell indicated Infection Inflammatory response less Meta-analysis Molecular dynamics simulation patients penetrate protective factor Protein pseudovirus entry SARS-CoV SARS-CoV-2 SARS-CoV-2 virus Spike protein structural basis temperature Temperature dependence the binding affinity the SARS-CoV-2 Vero viral infection Viral protein viral spike protein [DOI] 10.1016/j.csbj.2020.12.005 PMC 바로가기 [Article Type] Research Article