Inhibition of acid sphingomyelinase by ambroxol prevents SARS-CoV-2 entry into epithelial cells
Research Article
[키워드] ACE2, angiotensin-converting enzyme 2
acid sphingomyelinase
Activation
acute respiratory syndrome
addition
ambroxol
applied
ATCC, American Type Culture Collection
cells
cellular
ceramide
clinical study
coronavirus
COVID-19, Coronavirus disease 2019
DMEM, Dulbecco's modified Eagle's medium
DTPA, diethylenetriaminepentaacetic acid
eGFP, enhanced GFP
epithelial cell
epithelial cells
event
FCS, fetal calf serum
FIASMAs, functional inhibitors of the acid sphingomyelinase
fluorescence microscopy
H/S, Hepes/saline
human nasal epithelial cells
Infection
infection with SARS-CoV-2
inhalation
inhibit
inhibition
MEM, minimum essential medium
mimicking
nasal epithelial cell
NP-40, Nonidet P40
OGP, n -octyl-d -glucopyranoside
Particle
PFA, paraformaldehyde
pp-VSV-SARS-CoV-2, vesicular stomatitis virus pseudoviral particles presenting SARS-CoV-2 spike protein on their surface
presenting
Prevent
prevented
purified
quantified
reduced
required
restored
rhinovirus
SARS-CoV-2
SARS-CoV-2 entry
SARS-CoV-2 spike protein
SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2
shown
TMPRSS2, Transmembrane serine protease 2
vesicular stomatitis virus
viruses
Volunteer
[DOI] 10.1016/j.jbc.2021.100701 PMC 바로가기 [Article Type] Research Article
[DOI] 10.1016/j.jbc.2021.100701 PMC 바로가기 [Article Type] Research Article