Combined Subcutaneous-Intranasal Immunization With Epitope-Based Antigens Elicits Binding and Neutralizing Antibody Responses in Serum and Mucosae Against PRRSV-2 and SARS-CoV-2에피토프 기반 항원과 결합된 피하-비강내 면역화는 PRRSV-2 및 SARS-CoV-2에 대한 혈청 및 점막의 결합 및 중화 항체 반응을 유도합니다Article Published on 2022-03-312022-09-12 Journal: Frontiers in Immunology [Category] MERS, SARS, 진단, [키워드] Administered Against animal antibodies antibody Antigen antigens Antiviral approaches asymptomatic infection baculovirus binding binding antibody Combination Combined Concentration conserved elicit emerging and re-emerging viruses envelope protein epitope epitope-based antigens evaluate evaluated expressed fluids generate GP5 Humoral response immunization immunization strategy immunized induce Infection inhibited inoculated Interaction intranasal intranasally investigated Local memory response mucosal mucosal antibodies Mucosal immunization nasal Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody New Prevent produced promoted Protein RBD-ACE2 receptor-binding motif recombinant baculovirus recombinant proteins recombinant proteins. reduce response responses SARS-CoV-2 serum serum antibody serum sample subcutaneously the S protein Transmission vaccinated individuals Vaccine design vaginal swabs virus [DOI] 10.3389/fimmu.2022.848054 PMC 바로가기 [Article Type] Article
An easy pipeline for one-step purification of SARS-CoV-2 nucleocapsid protein from insect cell suspension culture곤충 세포 현탁액 배양에서 SARS-CoV-2 뉴클레오캡시드 단백질의 1단계 정제를 위한 간편한 파이프라인Article Published on 2022-01-012022-09-11 Journal: Journal of virological methods [Category] SARS, 변종, 치료기술, 치료법, [키워드] affinity Affinity chromatography average baculovirus buffer cellular response Cellular responses cloned COVID-19 COVID-19 pandemic Culture expression of SARS-CoV-2 followed by Genome high-density imidazole Infection insect cell insect cells N gene N protein nucleocapsid nucleocapsid protein One-step purification Protein purification purified resulting SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 variant SARS-CoV-2 variants Seroconversion serological test Serological tests Sf9 cell supernatant total protein Transposition tris Tris-HCl vaccination was used [DOI] 10.1016/j.jviromet.2021.114341 PMC 바로가기 [Article Type] Article
An Integrated Platform for Serological Detection and Vaccination of COVID-19COVID-19의 혈청학적 검출 및 백신 접종을 위한 통합 플랫폼Immunology Published on 2021-12-232022-09-11 Journal: Frontiers in Immunology [Category] SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome coronavirus adjuvant Animal experiment animal experiments anti-S anti-S antibody antibodies antibody Antibody Response Antigen baculovirus caused Cell complex coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients detect detectable detection detection system disease disease control effective elicited facilitate generate immunization Improvements insect insect cell insect cells maintain membrane mice Neutralizing antibodies neutralizing antibody nucleocapsid pandemic Patient platform Protein respiratory SARS-CoV-2 SARS-CoV-2 detection Serological detection serum severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 stimulated vaccination Vaccine Vaccines were used western blot [DOI] 10.3389/fimmu.2021.771011 PMC 바로가기 [Article Type] Immunology
Development of a recombinant vaccine containing a spike S1-Fc fusion protein induced protection against MERS-CoV in human DPP4 knockin transgenic miceArticle Published on 2021-10-302022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] addition alum adjuvant antigen-specific neutralizing antibody baculovirus BALB/c mice BSL, biosafety level CEF, chicken embryo fibroblast cells Chinese hamster CHO, Chinese hamster ovary coronavirus demonstrated development Dipeptidyl peptidase-4 DPP4 effective ELISA, enzyme-linked immunosorbent assay Extracellular family Coronaviridae Fc fragment Fc, fragment of crystalline Fragment of crystalline (Fc) molecule fused fusion protein genus Betacoronavirus highly pathogenic human DPP4 humoral immune response Humoral immunity IgG IgG4 immunogenicity inflammatory cell infiltration inoculated lack MERS-CoV MERS-CoV infection MERS-CoV, Middle East respiratory syndrome coronavirus MERS, Middle East respiratory syndrome mice Middle East Middle East respiratory syndrome Coronavirus Neutralizing antibodies PBS, phosphate-buffered saline PBST, phosphate-buffered saline in 0.05 % Tween 20 PCR, polymerase chain reaction PFU, plaque-forming unit Protective Protein Proteins RBD, receptor-binding domain respiratory syndrome coronavirus RLU, relative light unit S1 subunit SARS-CoV, severe acute respiratory syndrome coronavirus secretion SFM, serum-free medium Spike protein therapeutic transgenic mice Vaccine vaccine candidate Vaccine development viral challenge viral outbreak virus [DOI] 10.1016/j.jviromet.2021.114347 PMC 바로가기 [Article Type] Article
Robust Evaluation of Ultraviolet-C Sensitivity for SARS-CoV-2 and Surrogate CoronavirusesResearch Article Published on 2021-10-202022-10-30 Journal: Microbiology Spectrum [Category] COVID-19, MERS, SARS, [키워드] absorbance adopted baculovirus baculovirus vector collimated beam coronavirus COVID-19 pandemic Disinfection distribution dose Effectiveness eliminating Evidence for disinfection hazard HCoV-229E HCoV-OC43 highlight HuCoV 229E HuCoV OC43 inactivated inactivation indicator organism intensity irradiation medium methodology MHV mouse hepatitis virus optical organism reducing reduction SARS-CoV-2 sensitivity surrogate T1 bacteriophage Treatment ultraviolet UV light Variability virus [DOI] 10.1128/Spectrum.00537-21 PMC 바로가기 [Article Type] Research Article
Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologiesArticle Published on 2021-10-072023-08-30 Journal: Applied Microbiology and Biotechnology [Category] E형 간염, [키워드] AcMNPV BacMam baculovirus BEVS human diseases [DOI] 10.1007/s00253-021-11615-1 PMC 바로가기
Production of SARS-CoV-2 Virus-Like Particles in Insect CellsArticle Published on 2021-05-262022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] bacmid baculovirus cause Cell cloned competent COVID-19 disease Escherichia coli expression generate Health Infection insect insect cell insect cells morphology multiple cloning site Particle Plasmid platform Production promoter promoters S gene S protein SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 VLP virions virus-like particles VLPs were expressed [DOI] 10.3390/vaccines9060554 PMC 바로가기 [Article Type] Article
The Next Set of COVID-19 Vaccines: Leveraging New Development Platforms to Increase Access for More People Around the WorldReview Published on 2021-04-012023-08-30 Journal: Clinical therapeutics [Category] E형 간염, [키워드] baculovirus COVID recombinant adenovirus SARS-CoV-2 Vaccines [DOI] 10.1016/j.clinthera.2021.03.007 PMC 바로가기 [Article Type] Review
Human endogenous retrovirus-enveloped baculoviral DNA vaccines against MERS-CoV and SARS-CoV2Article Published on 2021-03-192022-10-30 Journal: NPJ Vaccines [Category] COVID-19, [키워드] acute respiratory syndrome animal models baculovirus Cell-mediated immunity Complete Coronavirus-2 COVID19 DNA DNA vaccine DNA vaccines domain envelope gene expressing feasibility gene delivery Genome Human human cells humoral immunized induce MERS-CoV Middle East NTD pandemic spread provided RBD respiratory syndrome coronavirus retrovirus S1 subunit SARS-CoV-2 SARS-CoV2 SARS-CoV2 RBD Support Vaccine Vaccines viral infections [DOI] 10.1038/s41541-021-00303-w PMC 바로가기 [Article Type] Article
Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus코로나 바이러스의 초고감도 검출 및 비활성화를 위한 앱타머 접합 금 나노스타 기반 거리 의존 나노입자 표면 에너지 전달 분광법Article Published on 2021-03-042022-09-11 Journal: The journal of physical chemistry letters [Category] SARS, 치료기술, [키워드] ACE2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Antigen aptamer baculovirus binding Conjugated Corona Coronavirus infection demonstrated detection Diagnosis DNA energy energy transfer GNS gold limit of detection membrane nanoparticle outbreak persist Protein Protein binding pseudotyped Receptor binding recombinant antigen rhodamine SARS-CoV-2 spike SARS-CoV-2 spike protein Spectroscopy Spike protein surface virus virus infection [DOI] 10.1021/acs.jpclett.0c03570 PMC 바로가기 [Article Type] Article