Flipped over U: structural basis for dsRNA cleavage by the SARS-CoV-2 endoribonuclease뒤집힌 U: SARS-CoV-2 엔도리보뉴클레아제에 의한 dsRNA 절단의 구조적 기초Article Published on 2022-08-122022-09-11 Journal: Nucleic Acids Research [Category] SARS, 유전자 메커니즘, 치료법, [키워드] activate Activation active site cleavage cleave coronavirus Coronavirus replication Critical cryo-EM structure cryo-EM structures double-stranded dsRNA endoribonuclease evade form generate Host immune MDA5 mechanism Nsp15 Pattern recognition pattern recognition receptor platform processing recognize replication-transcription complex RNA SARS-CoV-2 SARS-CoV-2 Nsp15 single-stranded site-directed mutagenesis structures substrates the SARS-CoV-2 uridine Vesicle viral replication [DOI] 10.1093/nar/gkac589 PMC 바로가기 [Article Type] Article
Sec61 Inhibitor Apratoxin S4 Potently Inhibits SARS-CoV-2 and Exhibits Broad-Spectrum Antiviral ActivitySec61 억제제 아프라톡신 S4는 SARS-CoV-2를 강력하게 억제하고 광범위한 항바이러스 활성을 나타냅니다Article Published on 2022-07-082022-09-11 Journal: ACS Infectious Diseases [Category] COVID19(2023년), SARS, 진단, 치료법, 치료제, [키워드] activity anticancer Antiviral antiviral activities antiviral activity blocked broad-spectrum antivirals cellular characterization COVID-19 Dengue dengue virus double-membrane vesicles dsRNA elicit Endoplasmic reticulum high potency Host host-directed therapeutics. human cells impacted in vitro in vivo Influenza influenza A influenza A virus inhibited inhibitor Microscopy Nile Pandemics Prevent prevented Protein Proteins reduced SARS-CoV-2 Sec61 shown Spike protein tested the spike protein translocation transmission electron microscopy vesicles Viral viral infection Viral protein viral replication virus West Zika [DOI] 10.1021/acsinfecdis.2c00008 PMC 바로가기 [Article Type] Article
Endothelial Cells as a Key Cell Type for Innate Immunity: A Focused Review on RIG-I Signaling Pathway선천성 면역을 위한 핵심 세포 유형으로서의 내피 세포: RIG-I 신호 전달 경로에 대한 집중 검토Review Published on 2022-07-052022-09-11 Journal: Frontiers in Immunology [Category] 치료법, [키워드] Activation anti-viral response Atherosclerosis Bacteria Bacterial bacterial and viral infections bloodstream cardiovascular disease cardiovascular diseases caused Cell cell type close contact contact with COVID-19 danger signals DDX58 demonstrated disorder disorders dsDNA dsRNA endothelial Endothelial cell endothelial cells Endothelial dysfunction Evidence expression function heterogeneous highlight host antiviral response immune immune functions immune surveillance Immunity Infectious disease Inflammation inhibited Innate Innate immunity interferons invading pathogen Ligand Like LPS macrophages microbial infection Panax notoginseng pathogen Pathogens PPARγ provided PRR recent receptor retinoic acid review RIG-I saponins shown signal signaling pathway Surveillance translational Type Vascular endothelium viral infection viral infections virus virus infection [DOI] 10.3389/fimmu.2022.951614 PMC 바로가기 [Article Type] Review
Discovery Proteomics Analysis Determines That Driver Oncogenes Suppress Antiviral Defense Pathways Through Reduction in Interferon-β Autocrine StimulationDiscovery Proteomics 분석을 통해 드라이버 종양유전자가 인터페론-β 자가분비 자극 감소를 통해 항바이러스 방어 경로를 억제한다는 사실이 확인되었습니다.Article Published on 2022-07-012022-09-11 Journal: Molecular & Cellular Proteomics : MCP [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, [키워드] activated AKT Antiviral approach autocrine BRAF c-MYC Cancer cancer cell line cancer cell lines cancers Cell cell line cellular DDX58 develop double-stranded double-stranded RNA downregulation driven by driving dsRNA EGFR expression functional hallmark HER2 immune evasion Infection interferon KRAS lower expression MEK molecular MYC OAS oncogene oncogenes. oncogenic osteosarcoma Pancreatic cancer phenotypic profiles Protein Proteins proteome provide reduced regulated response RIG-I RNA virus SARS-CoV-2 shown Stimulation susceptibility susceptibility to infection therapeutic treated type 1 interferon [DOI] 10.1016/j.mcpro.2022.100247 PMC 바로가기 [Article Type] Article
Sequence-Specific Features of Short Double-Strand, Blunt-End RNAs Have RIG-I- and Type 1 Interferon-Dependent or -Independent Anti-Viral Effects짧은 이중 가닥, 평활 말단 RNA의 서열 특이적 특징은 RIG-I 및 유형 1 인터페론 의존성 또는 독립적 항바이러스 효과를 갖는다Article Published on 2022-06-282022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, 치료제, [키워드] adaptors Adenovirus Anti-viral anti-viral effect Antiviral Antiviral agents Antiviral effect antiviral effects assays Cell cell cycle cell cycle genes cell line cells cellular complementary cytoplasmic DNA DNA virus downregulated dsRNA dsRNAs Effect elicited fibroblast Gene Expression H3N2 human airway IFN IFN-alpha IFN-I IFNAR2 independent independent of indicated Infection Influenza influenza A influenza A virus Influenza virus interferon knock-out library MDA5 mechanism mimicking molecular patterns MYD88 organism Organisms pathogen pathogen-associated molecular patterns Pathogens Randomized receptor required retinoic acid retinoic acid-inducible gene I RIG-I RNA RNA therapy RNA virus RNAs SARS-CoV-2 sequence short double-strand blunt-end RNA. transcriptomics transfection Type ZNFX1 [DOI] 10.3390/v14071407 PMC 바로가기 [Article Type] Article
Evidence of Infection of Human Embryonic Stem Cells by SARS-CoV-2SARS-CoV-2에 의한 인간 배아 줄기 세포 감염의 증거Article Published on 2022-06-102022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 cell types coronavirus described differentially expressed dsRNA embryonic embryos enterocytes Evidence evidence of Gut hepatocytes hESC Human immunofluorescence microscopy infect Infection interferon-stimulated gene interferon-stimulated genes ISGs life cycle low expression mechanism neurons question replicate Replication replication of SARS-CoV-2 reported Respiratory system RNA S protein SARS-CoV-2 SARS-CoV-2 receptors severe acute respiratory syndrome Coronavirus significantly STEM stem cell supernatant supernatants Support syncytium threshold upregulated variety Viral RNA viral titer viral titers virion virus were infected [DOI] 10.3389/fcimb.2022.911313 PMC 바로가기 [Article Type] Article
Discovery and Use of Long dsRNA Mediated RNA Interference to Stimulate Antiviral Protection in Interferon Competent Mammalian Cells인터페론 유능한 포유동물 세포에서 항바이러스 보호를 자극하기 위한 긴 dsRNA 매개 RNA 간섭의 발견 및 사용Article Published on 2022-05-062022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] Antiviral Antiviral effect antiviral response antiviral responses cell line cells cellular cleaved comparable competent complementary Concentration cornerstone CoV dependent on Dicer dsRNA Evidence explanation expressing fluorescent functional gene targeting generate green fluorescent protein HCoV HCoV-229E human coronavirus human coronaviruses IFN immune defense induce inducer inhibit Interference interferons knockdown Lineage Long long dsRNA mammalian mammalian cell mammalian cells Mediated mRNA pathway platform preventing infection produced protection Protein provide required responsible retained RISC RNA RNA interference RNA-induced silencing complex RNAi RNAi pathway SARS-CoV-2 replication sequence shown significantly siRNA siRNAs stimulate stomatitis suppress therapeutic These cells threshold type I interferon vesicular stomatitis vesicular stomatitis virus viral inhibition virus VSV-GFP [DOI] 10.3389/fimmu.2022.859749 PMC 바로가기 [Article Type] Article
The stalk domain of SARS-CoV-2 NSP13 is essential for its helicase activitySARS-CoV-2 NSP13의 줄기 도메인은 헬리카제 활성에 필수적입니다Article Published on 2022-04-232022-09-11 Journal: Biochemical and Biophysical Research Communication [Category] COVID19(2023년), SARS, 변종, 치료법, [키워드] affected ATPase binding ability caused connection COVID-19 demonstrated domain domains dsRNA effort enzymatic activity enzyme flexible linker Health Helicase highlight insertion involved lack linker nsp13 provide remained Rigid connection RNA SARS-CoV-2 SARS-CoV-2 nsp13 SARS-CoV-2 replication stalk thermal stability Transcription was impaired [DOI] 10.1016/j.bbrc.2022.02.068 PMC 바로가기 [Article Type] Article
Unraveling the antiviral activity of plitidepsin against SARS-CoV-2 by subcellular and morphological analysis세포내 및 형태학적 분석을 통한 SARS-CoV-2에 대한 플리티뎁신의 항바이러스 활성 규명Article Published on 2022-04-012022-09-11 Journal: Antiviral Research [Category] Coronavirus, COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] absence Affect Alpha Analysis antitumor agent Antiviral antiviral activity antiviral drugs Antiviral treatment approach biogenesis block caused Cell Complete coronavirus COVID-19 double-stranded RNA dsRNA Efficacy electron electron microscopy elongation factor eukaryotic translation evaluate the effect Extracellular genome replication group host protein infected Vero E6 cells Interaction labeling mechanism mechanism of action Microscopy morphogenesis Morphological morphological analysis New coronavirus new coronavirus SARS-CoV-2 offer organelle pandemic plasma membrane Plitidepsin Post-infection progeny Protein provided SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid shown Structure subcellular Support target TEM transmission electron microscopy treated unique Vero E6 Vero E6 cell VERO E6 cells Vesicle viral infection Viral particles viral replication virion virus viruses [DOI] 10.1016/j.antiviral.2022.105270 PMC 바로가기 [Article Type] Article
Virus-specific editing identification approach reveals the landscape of A-to-I editing and its impacts on SARS-CoV-2 characteristics and evolution바이러스 특정 편집 식별 접근 방식은 A-to-I 편집 환경과 SARS-CoV-2 특성 및 진화에 미치는 영향을 보여줍니다.Article Published on 2022-03-212022-09-11 Journal: Nucleic Acids Research [Category] SARS, 변종, 유전자 메커니즘, 치료법, [키워드] accelerated activate Affect antigenicity Antiviral approach atlas average cell types Characteristics component components correlated dsRNA event Evidence Evolution expression framework Genome highlight Host hotspot Human IFN response Impact induce infected with SARS-CoV-2 innate immune response intensity MDA5 provide provided regulated resource reveal RNA RNA virus RNA-seq data SARS-CoV-2 SARS-CoV-2 evolution SARS-COV-2 infection spike gene the epidemic tissue viral dsRNA viral infectivity Viral RNA [DOI] 10.1093/nar/gkac120 PMC 바로가기 [Article Type] Article