The stalk domain of SARS-CoV-2 NSP13 is essential for its helicase activitySARS-CoV-2 NSP13의 줄기 도메인은 헬리카제 활성에 필수적입니다Article Published on 2022-04-232022-09-11 Journal: Biochemical and Biophysical Research Communication [Category] COVID19(2023년), SARS, 변종, 치료법, [키워드] affected ATPase binding ability caused connection COVID-19 demonstrated domain domains dsRNA effort enzymatic activity enzyme flexible linker Health Helicase highlight insertion involved lack linker nsp13 provide remained Rigid connection RNA SARS-CoV-2 SARS-CoV-2 nsp13 SARS-CoV-2 replication stalk thermal stability Transcription was impaired [DOI] 10.1016/j.bbrc.2022.02.068 PMC 바로가기 [Article Type] Article
Multiplex quantification of C-terminal alpha-1-antitrypsin peptides provides a novel approach for characterizing systemic inflammationArticle Published on 2022-03-092022-10-04 Journal: Scientific Reports [Category] 바이오마커, [키워드] addition allergies alpha-1-antitrypsin approach Biomarker Biomarkers Blood C-terminal cohort of patient detect diagnostic disease Diseases EDTA enzymatic activity excessive immune response experiment FIVE Health healthy controls Human plasma in healthy individual in vitro in vivo infections initial knowledge Molecular medicine nine Occurrence peptide provide quantification Research Sepsis serine protease serum significantly higher systemic inflammation variety [DOI] 10.1038/s41598-022-07752-w PMC 바로가기 [Article Type] Article
Inhibition of ACE2-Spike Interaction by an ACE2 Binder Suppresses SARS-CoV-2 EntryACE2 바인더에 의한 ACE2-스파이크 상호작용의 억제는 SARS-CoV-2 진입을 억제합니다Article Published on 2022-03-072022-09-11 Journal: Angewandte Chemie (International ed. in English) [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] ACE2 ACE2 Inhibition Allostery binder Compound COVID-19 COVID-19 pandemic cultured cell cultured cells effective enzymatic activity host cell host cells impairing inhibit SARS-CoV-2 inhibited inhibiting inhibition Interaction middle modulating physiological functions PPI PPI Inhibition Prevent SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 Spike RBD SARS-CoV-2 variant SARS-CoV-2 variants small molecule suppressed therapeutic strategy Vaccine variant viral infection virus entry [DOI] 10.1002/anie.202115695 PMC 바로가기 [Article Type] Article
In silico screening-based discovery of novel covalent inhibitors of the SARS-CoV-2 3CL proteaseSARS-CoV-2 3CL 프로테아제의 새로운 공유 억제제의 실리코 스크리닝 기반 발견Article Published on 2022-03-052022-09-11 Journal: European journal of medicinal chemistry [Category] SARS, 치료제, [키워드] 3CL 3CL pro acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antiviral target approach carried catalytic complex Compound coronavirus coronavirus disease Coronavirus disease 2019 covalent binding covalent inhibitor COVID-19 crystal crystal structure database effective enzymatic activity evaluated example feasible identify inhibitor Interaction ligand binding pocket predicted protease provided residue SARS-CoV-2 SARS-CoV-2 3C-Like protease seeking severe acute respiratory syndrome Coronavirus small molecule the SARS-CoV-2 Treatment Virtual screening X-ray [DOI] 10.1016/j.ejmech.2022.114130 PMC 바로가기 [Article Type] Article
Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entryImatinib 및 methazolamide는 ACE2 효소 활성을 높이고 바이러스 진입을 억제하여 COVID-19 유발 대사 합병증을 개선합니다Article Published on 2022-03-012022-09-11 Journal: Cell Metabolism [Category] COVID19(2023년), SARS, 신약개발, 유전자 메커니즘, [키워드] ACE2 ACE2 binding ACE2 pathway Activation cardiovascular system Complication Compound coronavirus disease Coronavirus disease 2019 COVID-19 database disease enzymatic activator enzymatic activity harpagoside identify Imatinib impairment IMPROVE in vivo inhibited inhibiting insulin key factor kidney liver mechanism metabolic complication methazolamide methazolamide. multiple tissue multiple tissues Perturbation SARS-CoV-2 severe metabolic Spike protein systemic disease treat Treatment viral entry Virtual screening virus infection [DOI] 10.1016/j.cmet.2022.01.008 PMC 바로가기 [Article Type] Article
Obatoclax inhibits SARS-CoV-2 entry by altered endosomal acidification and impaired cathepsin and furin activity in vitroCoronaviruses Published on 2022-02-102022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, COVID19(2023년), SARS, [키워드] Acidic acute respiratory syndrome Affect antiviral activity Antiviral compound approach B.1.1.7 B.1.351 B.1.617.2 Beta blocked cathepsin L caused Cell clinical virus isolates Compound Concentration coronavirus Coronavirus disease 2019 COVID-19 cysteine protease Delta effective endocytosis Endosomal acidification enzymatic activity expressing furin furin protease global pandemic hACE2 HIV human Angiotensin-converting enzyme human lung in vitro inhibit intestinal cell MCL-1 mechanisms membrane fusion Obatoclax prevented Protein pseudotype reducing reporter S protein Safe SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 spike SARS-CoV-2 variant set off suggested the SARS-CoV-2 Treatment unmet need virion virus virus entry [DOI] 10.1080/22221751.2022.2026739 PMC 바로가기 [Article Type] Coronaviruses
Aloin isoforms (A and B) selectively inhibits proteolytic and deubiquitinating activity of papain like protease (PLpro) of SARS-CoV-2 in vitroArticle Published on 2022-02-092022-10-04 Journal: Scientific Reports [Category] Coronavirus, COVID19(2023년), MERS, SARS, 변종, 치료제, [키워드] 3CLpro Aloin Anti-viral coronavirus CoV Critical deubiquitinase Diseases effective enzymatic activity fluoride Host Hydrogen bond in vitro Infection Infectious diseases ingredient inhibit inhibited inhibiting inhibitory activity initial Interaction isoform isomer mechanism molecular MOST nasal nostril PLPro predicted protease proteolytic proteolytic activity replication of SARS-CoV-2 required responsible Salicylate SARS-CoV-2 Simulation study sodium Spread viral infection viral replication virus [DOI] 10.1038/s41598-022-06104-y PMC 바로가기 [Article Type] Article
Design, synthesis and in vitro evaluation of novel SARS-CoV-2 3CL pro covalent inhibitors새로운 SARS-CoV-2 3CL pro 공유 억제제의 설계, 합성 및 시험관 내 평가Article Published on 2022-02-052022-09-11 Journal: European journal of medicinal chemistry [Category] 신약개발, 치료제, [키워드] 3CL pro 3CLpro antiviral drug antiviral drugs coronavirus Coronavirus infection Coronavirus infections covalent inhibitors. COVID-19 pandemic cysteine demonstrated disease effort enzymatic activity in viral in vitro inhibitor inhibitors inhibitors of SARS-CoV-2 MERS MPro Outbreaks platform protease Replication reported SARS-CoV SARS-CoV-2 SARS-CoV2 starting point synthesis viral replication viruses was used were used [DOI] 10.1016/j.ejmech.2021.114046 PMC 바로가기 [Article Type] Article
Pro108Ser mutation of SARS-CoV-2 3CL pro reduces the enzyme activity and ameliorates the clinical severity of COVID-19SARS-COV-2 3CL PRO의 PRO108SER 돌연변이는 효소 활성을 감소시키고 COVID-19의 임상 적 심각성을 개선시킨다.Article Published on 2022-01-252022-09-10 Journal: Scientific Reports [Category] 변종, 유전자 메커니즘, 임상, 진단, 치료기술, 치료법, 치료제, [키워드] 3CL pro Amino acid amino acid residue characterization chymotrypsin Clinical course Clinical severity clinical trial Controlled clinical trial COVID-19 COVID-19 in patient death demonstrated deuterium enzymatic activity enzyme enzyme activity Enzyme mechanisms hospital hydrogen infected with SARS-CoV-2 Infection inhibitor International Lineage lower risk mass spectrometry mutant Mutation oxygen oxygen supplementation Patient patients with SARS-CoV-2 PF-07321332 Placebo positioned proportion protease Randomized reduce reduced required SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 strains strain Support symptom onset the patient treated Trial viral infection virus [DOI] 10.1038/s41598-022-05424-3 PMC 바로가기 [Article Type] Article
Small molecule interactions with the SARS-CoV-2 main protease: In silico all-atom microsecond MD simulations, PELE Monte Carlo simulations, and determination of in vitro activity inhibitionSARS-CoV-2 주요 프로테아제와의 소분자 상호작용: 인실리코 전 원자 마이크로초 MD 시뮬레이션, PELE 몬테카를로 시뮬레이션 및 시험관 내 활성 억제 결정Article Published on 2022-01-012022-09-11 Journal: Journal of molecular graphics & modelling [Category] MERS, SARS, 변종, 진단, 치료제, [키워드] active site acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Antiviral caused clinical evaluation clinical trials concentrations coronavirus COVID-19 COVID-19 pandemic Critical Cyanidin-3-O-glucoside demonstrated domain dose-dependent manner Effective vaccines enzymatic activity eradication experiments FDA GC376 glucoside highlight Hypericin in vitro in vitro activity inhibit inhibitor Interaction less Ligand ligands M pro main protease MD simulations molecular molecular dynamics Molecular modelling notable Orphan Drug performed positive control Previous studies protease respiratory Safe SARS-CoV-2 SARS-CoV-2 main protease severe acute respiratory syndrome Coronavirus situation small molecule stability the SARS-CoV-2 Trajectories Vaccine hesitancy variants of concern was used [DOI] 10.1016/j.jmgm.2021.108050 PMC 바로가기 [Article Type] Article