Receptor binding domain proteins of SARS-CoV-2 variants produced in Nicotiana benthamiana elicit neutralizing antibodies against variants of concernNicotiana benthamiana에서 생산된 SARS-CoV-2 변이체의 수용체 결합 도메인 단백질은 우려되는 변이체에 대한 중화 항체를 유도합니다Article Published on 2022-09-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (alpha Alpha Beta binding domain booster vaccination Booster vaccine cloned COVID-19 cross-reactive Day Delta Delta variants dose Effective vaccines elicit Epsilon evaluated expression vector Fc fragment fragment of Gamma gene encoding health problem human IgG1 immune response immunized immunogenicity induce intramuscularly mutant Mutation Neutralizing antibodies neutralizing antibody Neutralizing antibody response neutralizing antibody responses Nicotiana benthamiana plant-produced recombinant protein produced Protein public health RBD RBD-Fc RBD-Fc fusion protein Receptor-binding domain Recombinant protein required respiratory SARS-CoV-2 SARS-CoV-2 strains SARS-CoV-2 variant SARS-CoV-2 variants Severe acute respiratory syndrome significantly Spread subunit Subunit vaccine tested the receptor-binding domain the vaccine the vaccines transient expression Vaccine variant variant vaccine. variants variants of concern VOCs Wuhan [DOI] 10.1002/jmv.27881 PMC 바로가기 [Article Type] Article
Transient production of receptor-binding domain of SARS-CoV-2 in Nicotiana benthamiana plants induces specific antibodies in immunized miceNicotiana benthamiana 식물에서 SARS-CoV-2의 수용체 결합 도메인의 일시적인 생산은 면역된 마우스에서 특정 항체를 유도합니다Article Published on 2022-07-012022-09-12 Journal: Molecular biology reports [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] affected antibodies antigen expression benthamiana caused Cell coronavirus COVID-19 pandemic diagnostic system domain drug evaluate Host identity immune immunization immunized mice immunogenicity induce Interaction mice N. benthamiana Nicotiana benthamiana PBS plant platform produced Protein RBD RBD protein RBDr receptor ACE2 SARS-CoV-2 SARS-CoV-2 virus Scientific community specific antibody Spike protein spread of COVID-19 Support the receptor-binding domain the SARS-CoV-2 Transient treated vaccine candidates Vaccines viral surface [DOI] 10.1007/s11033-022-07402-4 PMC 바로가기 [Article Type] Article
Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnosticsArticle Published on 2022-02-012022-10-30 Journal: Journal of virological methods [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] American CDC Berlin can be used Charité Chinese CDC Combination Control COVID-19 Cowpea mosaic virus Diagnosis diagnostics Efficiency Genome gold standard hydrolysis in vivo In-tube control MONITOR multiplex RT-qPCR Multiplexing Nicotiana benthamiana novel nucleic acid extraction packaged Particle Patient PCR amplification plant virus polymerase chain positive control produced Production protocols Pseudogenome purified Reactions reagent reverse transcription RNA RNA extraction RT-qPCR SARS-CoV-2 sequence Specific swab sample transient expression virus virus particle Virus-like particle virus-like particles VLP [DOI] 10.1016/j.jviromet.2021.114372 PMC 바로가기 [Article Type] Article
In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan StructuresChemistry Published on 2022-02-012022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 receptor Affect Analysis antibody Antigen approach benthamiana binding Blood Group Blood group antigen carbohydrate cellular co-expressed complex contribute CR3022 domain ENhance Epitopes functional glycan glycoengineering glycosylated glycosylation group Human human sera IgG immune evasion immunogenic in vivo individual Interaction Modification N-glycan Nicotiana benthamiana non-infected performed plants posttranslational modification Production purified RBD recombinant SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 spike protein sera serological assays shown Structure structures Subunit vaccine the RBD the SARS-CoV-2 variant Viral antigen viral envelope protein virus virus infection virus transmission [DOI] 10.3389/fchem.2021.816544 PMC 바로가기 [Article Type] Chemistry
Reproducibility and flexibility of monoclonal antibody production with Nicotiana benthamianaNicotiana benthamiana를 사용한 단일 클론 항체 생산의 재현성 및 유연성Article Published on 2022-01-012022-09-12 Journal: mAbs [Category] 진단, [키워드] biologic clinical development demonstrated flexible good manufacturing practices Infection Infectious disease initiated mAb monoclonal antibody Nicotiana benthamiana outbreak pandemic plant Plant made pharmaceuticals plants platform produced reported reproducibility SARS-CoV-2 coronavirus Support Therapies These data ZMapp [DOI] 10.1080/19420862.2021.2013594 PMC 바로가기 [Article Type] Article
High-Yield Production of Receptor Binding Domain of SARS-CoV-2 Linked to Bacterial Flagellin in Plants Using Self-Replicating Viral Vector pEffArticle Published on 2021-12-062022-10-28 Journal: Plants [Category] COVID-19, [키워드] conditions coronavirus COVID-19 COVID-19 pandemic cytosol denaturing dialysis expressed expression flagellin fraction fused fusion protein Genome insoluble intranasal mucosal Ni-NTA Nicotiana benthamiana plant plants Production Protein purification purified RBD reached Receptor binding domain recombinant vaccine remained reported required Salmonella Typhimurium SARS-CoV-2 SARS-CoV-2 spike glycoprotein tissue Vaccine vector virus [DOI] 10.3390/plants10122682 PMC 바로가기 [Article Type] Article
Soluble Human Angiotensin- Converting Enzyme 2 as a Potential Therapeutic Tool for COVID-19 is Produced at High Levels In Nicotiana benthamiana Plant With Potent Anti-SARS-CoV-2 ActivityPlant Science Published on 2021-12-062022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] ACE2 ACE2 protein activity acute respiratory syndrome angiotensin Anti-SARS-CoV-2 Activity antiviral drug calculated candidate caused coronavirus coronavirus disease country COVID-19 demonstrated enzyme form global public health glycosylated glycosylated form high risk Human IC50 in vitro incubated Infection inhibitory concentration Level Ni-NTA Nicotiana benthamiana pandemic patients with COVID-19 plant plant transient expression potent Potential produced purification receptor Safe SARS-CoV-2 SARS-CoV-2 infections SARS-CoV-2 vaccine soluble ACE2 Spike protein spread to temperature the SARS-CoV-2 therapeutic therapeutic agent Treatment [DOI] 10.3389/fpls.2021.742875 PMC 바로가기 [Article Type] Plant Science
SARS-CoV-2 Spike Protein-Induced Interleukin 6 Signaling Is Blocked by a Plant-Produced Anti-Interleukin 6 Receptor Monoclonal AntibodyArticle Published on 2021-11-202022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] acute respiratory distress acute respiratory syndrome anti-IL-6 receptor anti-IL-6R caused coronavirus COVID-19 COVID-19 pandemic COVID-19 patients cytokine signaling Cytokine storm death Efficacy examined expressed feasibility homogeneity IL-6 IL-6 receptor (IL-6R) IL-6 signaling IL-6R in vitro model inhibit interleukin 6 interleukin 6 (IL-6) mAb monoclonal antibody monoclonal antibody (mAb) mononuclear cell Nicotiana benthamiana Peripheral blood plant plant-made antibody plant-made therapeutics producing Proinflammatory cytokine purification reducing sarilumab SARS-CoV-2 SARS-COV-2 infection shown spike spike protein (S) suppressed syndrome the spike protein utility [DOI] 10.3390/vaccines9111365 PMC 바로가기 [Article Type] Article
Production and Characterization of Nucleocapsid and RBD Cocktail Antigens of SARS-CoV-2 in Nicotiana benthamiana Plant as a Vaccine Candidate against COVID-19Article Published on 2021-11-172022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] antibody Antigen antigens Antiviral candidate characterization co-expressed co-expression cocktail COVID-19 COVID-19 pandemic COVID-19 vaccine demonstrated domain drugs effective elicited global public health high risk immunization immunized mAb mice monoclonal N protein Neutralizing activity Nicotiana benthamiana nucleocapsid nucleocapsid protein plant polyclonal antibody produced Production Protein purification purified RBD RBD of SARS-CoV-2 RBD-specific antibody reactivity Safe SARS-CoV-2 SARS-CoV-2 coronavirus strain Support therapeutic transient expression system vaccine candidate Vaccines variant virus [DOI] 10.3390/vaccines9111337 PMC 바로가기 [Article Type] Article
Increased in vitro neutralizing activity of SARS-CoV-2 IgA1 dimers compared to monomers and IgG단량체 및 IgG에 비해 SARS-CoV-2 IgA1 이량체의 시험관 내 중화 활성 증가Article Published on 2021-11-022022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Antigen antigen binding binding complex coronavirus exhibited expressed expression feature glycosylation homogeneous human proteins IgA IgG IgG1 in vitro Increased monoclonal monoclonal antibodies monomer Neutralizing activity neutralizing monoclonal antibodies neutralizing monoclonal antibody Nicotiana benthamiana plant plant expression plants respiratory SARS-CoV-2 SARS-CoV-2 neutralization severe acute respiratory syndrome Coronavirus significantly increased variant Variation [DOI] 10.1073/pnas.2107148118 PMC 바로가기 [Article Type] Article