Systematic profiling of SARS-CoV-2-specific IgG responses elicited by an inactivated virus vaccine identifies peptides and proteins for predicting vaccination efficacyArticle Published on 2021-08-172022-10-04 Journal: Cell Discovery [Category] 바이오마커, 비임상, 진단, [키워드] Antibody Response applied BBIBP-CorV China collected convalescent correlated COVID-19 COVID-19 patient Effectiveness elicited facilitate hospitalized COVID-19 patients Humoral response identify immunized immunology inactivated individual Microarray neutralization titer Nsp7 peptide Potential biomarker profile protective mechanism Protein Proteomic analysis response SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 vaccines SARS-CoV-2-specific IgG serum sample the microarray the N protein the spike protein the vaccine triggered vaccination vaccination efficacy Vaccine Vaccines virus Volunteer [DOI] 10.1038/s41421-021-00309-7 PMC 바로가기 [Article Type] Article
Transient and stabilized complexes of Nsp7, Nsp8, and Nsp12 in SARS-CoV-2 replicationSARS-COV-2 복제에서 NSP7, NSP8 및 NSP12의 과도 및 안정화 된 복합체Articles Published on 2021-08-032022-09-10 Journal: Biophysical Journal [Category] MERS, SARS, 유전자 메커니즘, [키워드] activities addition amplify atomic Combination complex complexation complexes component components conducted conformation Coordinate crystal structure crystal structures Crystallography defined Disruption DNA form functions greater high-resolution crystal structure Host host immune responses immune responses Interaction make up mechanism mRNA new virus Nsp12 Nsp7 NSP8 Propagation Protein Proteins recognizing Replication reported responsible RNA RNA-dependent RNA polymerase RTC SANS SARS-CoV-2 SARS-CoV-2 replication SAXS scattering size exclusion chromatography Structure Transcription Transient viability Viral Viral proteins Viral RNA virus [DOI] 10.1016/j.bpj.2021.06.006 PMC 바로가기 [Article Type] Articles
In silico analysis of RNA-dependent RNA polymerase of the SARS-CoV-2 and therapeutic potential of existing antiviral drugsSARS-CoV-2의 RNA 의존성 RNA 중합효소의 in silico 분석 및 기존 항바이러스제의 치료 가능성Article Published on 2021-08-012022-09-11 Journal: Computers in biology and medicine [Category] SARS, 치료제, [키워드] alteration Amino acid amino acids Analysis Antiviral antiviral drug antiviral drugs binding affinity cofactor complex Computational biology conserved coronavirus demonstrated effective highlight homologous hydrogen Hydrogen bond hydrogen bonds Infection Interaction interaction site Intervention involved mode Molecular modelling molecules Multiple Multiple sequence alignment mutable Nsp12 Nsp7 Organisms phylogenetic analyses Phylogenetic analysis RaTG13 RdRP reduced Region remained Remdesivir resulting RNA RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 virus selected selected drugs sequence Sequence alignment sofosbuvir subunit subunits sustained the binding affinity the SARS-CoV-2 the SARS-CoV-2 virus therapeutic potential therapeutic strategy uracil Viral RNA [DOI] 10.1016/j.compbiomed.2021.104591 PMC 바로가기 [Article Type] Article
Antibody landscape against SARS-CoV-2 reveals significant differences between non-structural/accessory and structural proteinsSARS-CoV-2에 대한 항체 환경은 비구조적/부속적 및 구조적 단백질 간의 상당한 차이를 보여줍니다Article Published on 2021-07-132022-09-12 Journal: Cell Reports [Category] MERS, SARS, 진단, [키워드] accessory protein accessory proteins Antibody Response Clinical outcome COVID-19 COVID-19 sera death decrease disease severity elicit facilitate humoral Humoral immunity IgG IgG antibody IgG response IgG responses IgM immune immune response Immunoglobulin immunology Microarray N protein N proteins non-structural protein non-structural/accessory proteins Non-survivors nsp1 Nsp7 NSP8 ORF3b Orf9b prevalent Protein proteome proteome microarray. RdRP reveal SARS-CoV-2 sera significant difference structural protein symptom onset the SARS-CoV-2 [DOI] 10.1016/j.celrep.2021.109391 PMC 바로가기 [Article Type] Article
Two conserved oligomer interfaces of NSP7 and NSP8 underpin the dynamic assembly of SARS-CoV-2 RdRPNSP7 및 NSP8의 2개의 보존된 올리고머 인터페이스는 SARS-CoV-2 RdRP의 동적 조립을 뒷받침합니다.AcademicSubjects/SCI00010 Published on 2021-06-042022-09-10 Journal: Nucleic Acids Research [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] (RdRp Affect antiviral drug assays assembly biochemical complex complexes conserved coronavirus Critical crystal structure Formation Genome heterodimeric identify impairment implication Interaction interface mechanism Mutagenesis Mutation non-structural protein non-structural proteins Nsp12 Nsp7 NSP8 Protein provide RdRP Replication responsible reveal RNA polymerase RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 genome selective solution stability the SARS-CoV-2 genome [DOI] 10.1093/nar/gkab370 PMC 바로가기 [Article Type] AcademicSubjects/SCI00010
Piece of the puzzle: Remdesivir disassembles the multimeric SARS-CoV-2 RNA-dependent RNA polymerase complex퍼즐 조각: Remdesivir는 다량체 SARS-CoV-2 RNA 의존성 RNA 중합효소 복합체를 분해합니다Article Published on 2021-06-012022-09-11 Journal: Cell biochemistry and biophysics [Category] SARS, 치료제, [키워드] Accelerated molecular dynamics simulation adenosine ATP binding complex computational simulations contribute Detachment disrupt domain domains finding flexible followed by global mortality higher affinity hosts Human mechanism molecular molecular dynamics Molecular dynamics simulation non-structural proteins Nsp7 NSP8 nucleotide PALM Perturbation piece RdRP Remdesivir Replication replicative reported RNA RNA-dependent RNA polymerase SARS-CoV-2 SARS-like coronavirus significantly Spread structure-based design subunit the SARS-CoV-2 underlie Viral RNA virus [DOI] 10.1007/s12013-021-00977-y PMC 바로가기 [Article Type] Article
Genome sequencing of SARS-CoV-2 in a cohort of Egyptian patients revealed mutation hotspots that are related to clinical outcomesArticle Published on 2021-04-282023-06-16 Journal: Biochimica et biophysica acta. Molecular basis of [Category] MERS, SARS, [키워드] Clinical symptoms Mutation nsp6, nsp13-helicase Nsp7 SARS-CoV-2 Spike glycoprotein signal peptide [DOI] 10.1016/j.bbadis.2021.166154 PMC 바로가기 [Article Type] Article
Genetic conservation of SARS-CoV-2 RNA replication complex in globally circulating isolates and recently emerged variants from humans and minks suggests minimal pre-existing resistance to remdesivir전 세계적으로 순환하는 분리주와 최근에 출현한 인간과 밍크 변이체에서 SARS-CoV-2 RNA 복제 복합체의 유전적 보존은 렘데시비르에 대한 최소한의 기존 내성을 시사합니다Article Published on 2021-04-012022-09-11 Journal: Antiviral Research [Category] SARS, 변종, 유전자 메커니즘, [키워드] active site addition Amino acid amino acid substitution analyzed antiviral activity approved circulating complex coronavirus COVID-19 Critical Efficacy exhibit Frequency Genetic genetic diversity Genetic variation highest Human in vitro isolate isolates mechanism molecular target Mutation nsp10 Nsp12 nsp12 RdRp. nsp13 nsp14 Nsp7 NSP8 P323L polymerase RdRP RDV Remdesivir Replication resistance risk RNA RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 sequence SARS-CoV-2 variant SARS-CoV-2 variants selected sequence sequence variation sequences South Africa Spread stability Treatment treatment of COVID-19 United Kingdom variant variants virus [DOI] 10.1016/j.antiviral.2021.105033 PMC 바로가기 [Article Type] Article
Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients기능적 SARS-CoV-2-특이적 T 세포의 조기 유도는 COVID-19 환자의 빠른 바이러스 제거 및 경증 질병과 관련이 있습니다.Case Reports Published on 2021-02-092022-09-12 Journal: Cell Reports [Category] MERS, 진단, [키워드] accelerated Acute phase4 acute SARS-CoV-2 infection antibodies antibody cellular immunity change circulating convalescence COVID-19 patient death disease disease onset disease severity functional Host humoral Humoral response immune immunological parameter implication increase in interferon longitudinal membrane Mild Nsp7 ORF3a Patient Prognostic value PROTECT Proteins respiratory tract SARS-CoV-2 viral RNA SARS-CoV-2-specific T cell Support symptomatic T cell T cell response Vaccine design viral clearance viral infection Virological with mild disease [DOI] 10.1016/j.celrep.2021.108728 PMC 바로가기 [Article Type] Case Reports
Different mutations in SARS-CoV-2 associate with severe and mild outcomeSARS-CoV-2의 다양한 돌연변이는 중증 및 경미한 결과와 관련이 있습니다.Article Published on 2021-02-012022-09-11 Journal: International journal of antimicrobial agents [Category] SARS, 변종, 치료법, [키워드] alteration Amino acid amino acid change amino acid changes applied approach association Chi-square chi-square test Clinical outcome correlated correlation Cutoff death disease evaluated false discovery rate FDR Genome Genomic alterations GISAID glycoprotein help high-risk Hospitalization Hypothesis identify in viral lead mapped Mild Mild disease Mutation mutations next generation sequencing Nsp3 nsp4 NSP6 Nsp7 nucleocapsid Nucleocapsid phosphoprotein ORF3a ORF6 ORF8 outcome Patient phosphorylated Prevalence Proteins Repository RNA RNA dependent RNA polymerase RNA polymerase SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 virus sequence Sequencing severe disease the SARS-CoV-2 virus Treatment Viral viral gene viral genes viral genome viral sequences virus [DOI] 10.1016/j.ijantimicag.2020.106272 PMC 바로가기 [Article Type] Article