Discovery of Novel and Highly Potent Inhibitors of SARS CoV-2 Papain-Like Protease Through Structure-Based Pharmacophore Modeling, Virtual Screening, Molecular Docking, Molecular Dynamics Simulations, and Biological EvaluationPharmacology Published on 2022-02-212022-10-31 Journal: Frontiers in Pharmacology [Category] COVID-19, [키워드] Analysis anti-viral drug antiviral immune responses approach Biological carried complexes Compound compounds conducted COVID-19 database Dynamics dysregulation effective Host identify indicated Inflammation inhibitor inhibitory activity Interaction key amino acid maturation MD simulation modeling molecular docking molecular dynamics novel Papain-like protease pharmacophore pharmacophore modeling PLPro positive control potent predicted protease SARS CoV-2 Screening stability Treatment virus was used [DOI] 10.3389/fphar.2022.817715 PMC 바로가기 [Article Type] Pharmacology
Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing ProtocolMedicine Published on 2022-02-172022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] Amplicon amplicon drop-offs amplified ARTIC Network ARTIC V4 average Beta characterized clinical sample Concentration correlation Delta delta variant Eta Genome genome coverage IMPROVE increase in instance optimization Oxford Nanopore Technology positive control positive controls primer Primers protocol released Result SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 sequencing SARS-CoV-2 variants sequenced statistically significant the SARS-CoV-2 V3 primer set variant Whole genome sequencing [DOI] 10.3389/fmed.2022.836728 PMC 바로가기 [Article Type] Medicine
Elevated Cytokine Levels in Plasma of Patients with SARS-CoV-2 Do Not Contribute to Pulmonary Microvascular Endothelial PermeabilitySARS-CoV-2 환자의 혈장에서 증가된 사이토카인 수준은 폐 미세혈관 내피 투과성에 기여하지 않습니다Research Article Published on 2022-02-162022-09-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, [키워드] accounted ACE-2 receptor ACE2 antibody acute respiratory distress acute respiratory distress syndrome acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 acute SARS-CoV-2 infection angiopoietin antagonists antiinflammatory cytokines appear approach ARDS barrier dysfunction C3a C5a cause Cell changes in circulating Combination complement complements factors consecutive patient Control controls coronavirus cytokine Cytokines death demonstrated determine disrupt Effect eight Elevation endothelial Endothelial cell endothelial injury endothelial permeability enrolled evaluated Evidence evidence of Frequency greater growth factor heat inactivation human lung in vitro infections inhibitors labile Level lung magnitude mechanism Microvascular damage morbidity morbidity and mortality multiorgan failure Neutralizing antibodies neutralizing antibody occur patients patients hospitalized patients with SARS-CoV-2 permeability plasma positive control predicted produced proinflammatory cytokines provide pulmonary receptor receptor antagonists respiratory respiratory distress SARS-CoV-2 SARS-CoV-2 infected patients SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 patient SARS-CoV-2 patients SARS-CoV-2 plasma selected severe acute respiratory syndrome Coronavirus severe complication severe complications Spike protein subsequent susceptible syndrome the SARS-CoV-2 thrombin TNF-α Tumor tumor necrosis tumor necrosis factor tumor necrosis factor-alpha Variability Vascular vascular endothelial growth factor Vascular injury vascular permeability VEGF [DOI] 10.1128/spectrum.01671-21 PMC 바로가기 [Article Type] Research Article
Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnosticsArticle Published on 2022-02-012022-10-30 Journal: Journal of virological methods [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] American CDC Berlin can be used Charité Chinese CDC Combination Control COVID-19 Cowpea mosaic virus Diagnosis diagnostics Efficiency Genome gold standard hydrolysis in vivo In-tube control MONITOR multiplex RT-qPCR Multiplexing Nicotiana benthamiana novel nucleic acid extraction packaged Particle Patient PCR amplification plant virus polymerase chain positive control produced Production protocols Pseudogenome purified Reactions reagent reverse transcription RNA RNA extraction RT-qPCR SARS-CoV-2 sequence Specific swab sample transient expression virus virus particle Virus-like particle virus-like particles VLP [DOI] 10.1016/j.jviromet.2021.114372 PMC 바로가기 [Article Type] Article
The anti-HCV, Sofosbuvir, versus the anti-EBOV Remdesivir against SARS-CoV-2 RNA dependent RNA polymerase in silicoSARS-CoV-2 RNA 의존성 RNA 중합효소에 대한 항-HCV, Sofosbuvir 대 항-EBOV Remdesivir in silicoArticle Published on 2022-02-012022-09-11 Journal: Molecular diversity [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] active form affecting Anti-HCV antiviral drugs approved binding binding affinity binding energy binding mechanism clinically comparable complexes Control coronavirus diseases Coronaviruses COVID-19 disease docked docking drug Drug repurposing Effectiveness Efficacy FDA HCV Health help in silico inhibitor Interaction Ligand limit molecular docking molecular dynamics Molecular dynamics simulation Nsp12 nsp12 protein nucleotide polymerases positive control Protein RdRP Remdesivir reported RNA polymerase RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 RdRp SARS-CoV-2 RNA sofosbuvir target tested the binding affinity the SARS-CoV-2 therapeutic target three-dimensional structure used to evaluate Viral protein [DOI] 10.1007/s11030-020-10178-z PMC 바로가기 [Article Type] Article
A study of quality assessment in SARS-CoV-2 pathogen nucleic acid amplification tests performance; from the results of external quality assessment survey of clinical laboratories in the Tokyo Metropolitan Government external quality assessment program in 2020SARS-CoV-2 병원체 핵산 증폭 시험 성능의 품질 평가 연구; 2020년 도쿄도 외부 품질 평가 프로그램의 임상 실험실에 대한 외부 품질 평가 조사 결과Article Published on 2022-02-012022-09-11 Journal: Journal of infection and chemotherapy : official j [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] adopted amplification automated company conducted COVID-19 cycle Detection limit equipment External external quality control False negative False positive implementation Influenza Influenza virus Laboratory LAMP Loop-mediated isothermal amplification Loop-mediated isothermal amplification (LAMP) method NAAT name negative control non-specific nucleic acid nucleic acid amplification nucleic acid amplification testing nucleic acid extraction nucleic acid extraction kit participant pathogen positive control positive sample Quality control real-time RT-PCR reported RT-PCR SARS-CoV-2 target gene Tokyo virus genome [DOI] 10.1016/j.jiac.2021.10.027 PMC 바로가기 [Article Type] Article
Computational Analysis Reveals Monomethylated Triazolopyrimidine as a Novel Inhibitor of SARS-CoV-2 RNA-Dependent RNA Polymerase (RdRp)Article Published on 2022-01-262022-10-30 Journal: Molecules [Category] COVID-19, SARS, [키워드] absence against SARS-CoV analogue analysed antiviral activity Compound condition docked drug drug candidate drugs effective inhibitor essramycin Favipiravir Genomic variability HCV HIV in vitro in vivo influenza viruses inhibitor inhibitory activity knowledge Meta-analysis molecular molecular docking more difficult mutants non-structural protein non-structural proteins (NSP) novel nsp1 nsp5 NSP8 outbreak performed pharmacological positive control potential therapeutic agent Protein RdRP RdRp protein Remdesivir respiratory RNA RNA polymerase RNA-dependent RNA polymerase RNA-dependent RNA polymerase (RdRp) SARS-CoV-2 stability the SARS-CoV-2 triazolopyrimidine Vaccine viral replication virus was performed [DOI] 10.3390/molecules27030801 PMC 바로가기 [Article Type] Article
A short plus long-amplicon based sequencing approach improves genomic coverage and variant detection in the SARS-CoV-2 genomeResearch Article Published on 2022-01-132022-10-28 Journal: PLoS ONE [Category] COVID-19, [키워드] Amplicon amplify Analysis Combined Coverage COVID-19 pandemic COVID-19 positive patients Deletion deletions E484K eight evaluated example final set fraction Genome Genome sequencing genomic hybrid IMPROVE insertion insertions interval intervals K417N Multiplex-PCR Mutation mutations N501Y nasopharyngeal swab specimen nonsynonymous ORF3a protein Patient positive control primer Primers Protein provide RNA SARS-CoV-2 SARS-CoV-2 lineage SARS‐CoV‐2 Sequencing sequencing approach significantly Single nucleotide variant SNVs spike gene synonymous the SARS-CoV-2 genome variant variants of concern Viral transmission virus genome [DOI] 10.1371/journal.pone.0261014 PMC 바로가기 [Article Type] Research Article
Drug similarity and structure-based screening of medicinal compounds to target macrodomain-I from SARS-CoV-2 to rescue the host immune system: a molecular dynamics study숙주 면역계를 구하기 위해 SARS-COV-2로부터 마크로 도메인 -I를 표적화하기위한 약용 화합물의 약물 유사성 및 구조 기반 스크리닝 : 분자 역학 연구Article Published on 2022-01-012022-08-31 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 치료제, [키워드] active site ADPr Analysis approaches cause caused Compound coronavirus Critical docking docking score drug drug similarity drug target free energy Free energy calculations help Host host immune system identify immune immune system in viral Infection inhibit Interaction macrodomain Macrodomain-I molecular molecular dynamics Molecular dynamics simulation outbreak positive control public health repeated Replication reported researcher residue residues risk SARS-CoV-2 screened severe pneumonia significantly similarity small molecule small molecule inhibitor Small molecule inhibitors structure-based screening targets the disease these compound these compounds vaccine candidate vaccine candidates Viral viral replication Virtual screening Wuhan Wuhan, China [DOI] 10.1080/07391102.2020.1815583 PMC 바로가기 [Article Type] Article
An undergraduate laboratory experiment with real-world applications: Utilizing templateless polymerase chain reaction and real-time polymerase chain reaction to test for SARS-CoV-2 RNA실제 적용을 위한 학부 실험실 실험: 템플릿이 없는 중합효소 연쇄 반응과 실시간 중합효소 연쇄 반응을 활용하여 SARS-CoV-2 RNA 테스트Article Published on 2022-01-012022-09-11 Journal: Biochemistry and Molecular Biology Education [Category] SARS, 치료기술, [키워드] Biochemistry Biotechnology Chain Reaction Combination exercise experiment experimental protocols independent Laboratory laboratory exercise. Molecular biology polymerase chain polymerase chain reaction positive control primer Primers protocols Research RNA RNA extraction RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV2 [DOI] 10.1002/bmb.21593 PMC 바로가기 [Article Type] Article