Identification of Small Molecule Inhibitors of the Deubiquitinating Activity of the SARS-CoV-2 Papain-Like Protease: in silico Molecular Docking Studies and in vitro Enzymatic Activity AssayChemistry Published on 2020-12-082022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] activity Antiviral Antiviral effect approach binding binding site candidate canonical catalytic caused Characteristics cleave Compound compounds Consequences coronavirus COVID-19 Critical crystal structures deubiquitinase deubiquitinase inhibitors docking Effects enzymatic activity evaluate functional gallate high affinity Hypericin identification immune regulation in silico in vitro indicated inhibited inhibiting inhibitor inhibitors mechanism molecular docking molecule pandemic Papain-like protease polypeptides pp1a pp1ab protease Protease activity Protease inhibitor Proteases public health Replication SARS-CoV-2 Seven shown small molecule subsequent the SARS-CoV-2 the SARS-CoV-2 virus these compound trials [DOI] 10.3389/fchem.2020.623971 PMC 바로가기 [Article Type] Chemistry
Identification of Lysine Acetylation Sites on MERS-CoV Replicase pp1abResearch Published on 2020-11-232022-10-30 Journal: Molecular & Cellular Proteomics : MCP [Category] Coronavirus, [키워드] acetylated acetylation Affect Analysis Bioinformatic bioinformatics conserved coronavirus disease enzyme activity event events expressing facilitate Factor Factors HDAC HEK293 cells Host host response identification immunoaffinity immunoprecipitation infected cell life-threatening mechanism MERS MERS-CoV NCBI database occur pandemic peptide peptides polyprotein post-translational modifications pp1ab predict predicted Protein regulate Regulatory replicase Replication reported responsible RNA virus sequence SIRT1 Site subject upstream viral infection Viral protein viral replication viruses was performed western blotting [DOI] 10.1074/mcp.RA119.001897 PMC 바로가기 [Article Type] Research
Room-temperature neutron and X-ray data collection of 3CL M pro from SARS-CoV-2Research Communications Published on 2020-10-012022-10-28 Journal: Acta Crystallographica. Section F [Category] COVID-19, MERS, SARS, [키워드] 3CL 3CL Mpro catalytic catalytic dyad catalytic site cleavage collected Cys145 cysteine protease described determine enzyme functional His41 inactive information M pro mechanism neutron diffraction polyproteins pp1a pp1ab Proteins protonation state replication of SARS-CoV-2 residue SARS-CoV-2 X-ray X-ray data collection X-ray diffraction [DOI] 10.1107/S2053230X20011814 PMC 바로가기 [Article Type] Research Communications
Arterivirus nsp12 versus the coronavirus nsp16 2′- O -methyltransferase: comparison of the C-terminal cleavage products of two nidovirus pp1ab polyproteinsStandard Published on 2015-09-012022-10-30 Journal: The Journal of General Virology [Category] Coronavirus, [키워드] acting analysed Analysis aromatic residue arteritis arterivirus assumed biochemical assay Bioinformatics analysis C-terminal cleavage conserved coronavirus detect domain ENCODE enzyme equine family Coronaviridae genetics implicated knowledge Methylation mRNA mRNAs Nidovirale nidovirus Nsp12 ORF1b phenotype polyprotein pp1ab Protein Proteins Replication similarity statistically significant Substitution subunit transcripts tyrosine virus yielding β-strands [DOI] 10.1099/vir.0.000209 PMC 바로가기 [Article Type] Standard