Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
Production of single-cycle infectious SARS-CoV-2 through a trans-complemented repliconArticle Published on 2022-12-012022-11-16 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome approach BAC Cell cells clone coated Coronavirus-2 COVID-19 culture supernatant did not produce elicit expression generate IFN immune response infected cell Infection Infectious virus Live attenuated vaccine memory N protein nucleocapsid omicron overexpression plaques platform Production progeny provide Replication replicon rescued resulting RNA robust S protein SARS-CoV-2 single-cycle infectious virus. Strains transient expression transmission electron microscopy type I interferon Vaccine Viral RNA virion virus [DOI] 10.1002/jmv.28057 PMC 바로가기
A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccineArticle Published on 2022-12-012022-11-15 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acute respiratory syndrome analysed antibody antibody titres BALB/c BALB/c mouse boost CD4 CD8 cellular cellular immunity coronavirus coronavirus disease COVID-19 COVID-19 vaccine Delta/B.1.617.2 variant of concern effective eight elicited emerging and re-Emerging coronaviruses glycoprotein homologous humoral Humoral immunity immunization Japanese LC16m8 less mice motif multifunctional neutralized Neutralizing activity patients with COVID-19 polybasic prime-boost immunization. provide recombinant replication-competent viral S-specific antibody S1/S2 cleavage site SARS-CoV-2 serum sample significantly lower Strains T-cell immune responses T-cell immunity T-cell Response the S protein Vaccine Vaccine development vaccine platform variant vector viral vectors virus Wuhan [DOI] 10.1080/22221751.2022.2122580 PMC 바로가기
Longevity of immunity following COVID-19 vaccination: a comprehensive review of the currently approved vaccinesCOVID-19 백신 접종 후 면역 장수: 현재 승인된 백신에 대한 종합 검토Review Published on 2022-11-302022-09-12 Journal: Human vaccines & immunotherapeutics [Category] MERS, 진단, [키워드] Ad26.COV2.S anti-Spike IgG antibody antibody persistence approved Asymptomatic AZD1222 BNT162b1 BNT162b2 BNT162b2 vaccine BNT162b2 vaccines booster dose Cell-mediated immunity COVID-19 COVID-19 vaccination detectable first dose Humoral immunity IgA antibodies immune response Immunity immunogenicity induce long-term immunity Long-term protection Longevity mRNA-1273 mRNA-1273 vaccine neutralizing effect peaked persistence Perspective produced provide SARS-CoV-2 Spread systematic review T-cell T-cell immunity T-cell Response Vaccine Vaccines. [DOI] 10.1080/21645515.2022.2037384 PMC 바로가기 [Article Type] Review
One-click investigation of shape influence of silver nanostructures on SERS performance for sensitive detection of COVID-19Article Published on 2022-11-222022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, [키워드] Ag nanostructure approach Concentration COVID-19 dendritic Diagnosis Diseases indicated investigated magnitude Microdroplets array Mini-pillar platform morphology multiple parameter Novel coronavirus provide reproducibility RNA sequence SARS-CoV-2 sensitive sensitivity SERS. the SARS-CoV-2 transfer [DOI] 10.1016/j.aca.2022.340523 PMC 바로가기
Comprehensive Fragment Screening of the SARS-CoV-2 Proteome Explores Novel Chemical Space for Drug DevelopmentArticle Published on 2022-11-142022-11-15 Journal: Angewandte Chemie (International ed. in English) [Category] COVID19(2023년), SARS, 변종, 신약개발, 치료기술, 치료제, [키워드] binding binding site Chemical chemotypes Comprehensive Consensus COVID19-NMR development drug drug design Drug discovery fragment fragment screening functional identify International Intervention Ligand NMR NMR spectroscopy novel performed predict protease Protein Proteins proteome protocol provide public health RNA genome SARS-CoV-2 SARS-CoV-2. Screening Space the SARS-CoV-2 Vaccines variant variants of concern was used [DOI] 10.1002/anie.202205858 PMC 바로가기
Evaluating correlates of protection for mix-match vaccine against COVID-19 VOCs with potential of evading immunityArticle Published on 2022-11-082022-11-15 Journal: Vaccine [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] appear Bayesian Beta ChAdOx1 Clinical efficacy Correlate of protection COVID-19 D614G Delta Delta VOC distribution Efficacy ENhance evaluate Evaluating finding Heterologous highlight homologous Immunity inversion Mix-match mRNA-1273 neutralizing antibody omicron pandemic phase 3 trial predicted Predictive previous study provide ranged reduction reductions in strain vaccination Vaccine variant Variant of concern. variants variants of concern VoC VOCs was used while Wuhan [DOI] 10.1016/j.vaccine.2022.10.011 PMC 바로가기
Characterization and immunogenicity of SARS-CoV-2 spike proteins with varied glycosylationArticle Published on 2022-11-082022-11-15 Journal: Vaccine [Category] SARS, 진단, [키워드] 293 cells 293F cell acute respiratory syndrome addition binding caused Cell changed characterization convalescent sera coronavirus Coronavirus disease-19 COVID-19 Critical discrepancy effective Efficacy elicited exhibited expression globe glycosylated glycosylation glycosylation pattern homogeneity host cells IgG immunization immunogenicity insect cell mammalian cells mice Neutralizing antibody response neutralizing antibody titre pandemic Protein Proteins provide reactivity Receptor binding regimen robust S protein S-2P SARS-CoV-2 SARS-CoV-2 spike protein spike Spike. the S protein thermal stability Vaccine Vaccine design Variation virus virus entry while [DOI] 10.1016/j.vaccine.2022.09.057 PMC 바로가기
Evaluation of commercially available fully automated and ELISA-based assays for detecting anti-SARS-CoV-2 neutralizing antibodiesArticle Published on 2022-11-082022-11-15 Journal: Scientific Reports [Category] SARS, 치료기술, [키워드] acute respiratory syndrome administration affecting analysis Analysis anti-RBD IgG antibody anti-SARS-CoV-2 AUC automated binding antibody booster clinical settings Cohort collected Control coronavirus correlation COVID-19 cutoff value demonstrated Effectiveness Genscript gold standard Immunity immunoassay in both groups infected patient Mindray NAb neutralization Neutralization assay neutralization test neutralizing antibody population level protective immunity provide pseudovirus R 2 Rapid receiver operating characteristic Research ROC ROC Curve Sample SARS-CoV-2 SARS-CoV-2 infected patient second dose sensitivity shown significantly specificity subjects sVNT sVNT assay symptoms onset tested threshold vaccinated individual Vaccine virus neutralization test was performed [DOI] 10.1038/s41598-022-21317-x PMC 바로가기
ACE2 N-glycosylation modulates interactions with SARS-CoV-2 spike protein in a site-specific mannerArticle Published on 2022-11-052022-11-16 Journal: Communications Biology [Category] SARS, 변종, [키워드] accumulated ACE2 ACE2 receptor binding binding site caused cellular changes in conserved Delta glycan glycosylation glycosylation deletion glycosylation site Guidance Interaction investigated lineages modulate mutated N-glycosylation omicron parental profile provide SARS-CoV-2 SARS-CoV-2 spike protein soluble ACE2 spike spike mutation the SARS-CoV-2 therapeutic use variant variants of concern virus VOCs [DOI] 10.1038/s42003-022-04170-6 PMC 바로가기