Analytical performances of the AMPLIQUICK ® Respiratory Triplex assay for simultaneous detection and differentiation of SARS-CoV-2, influenza A/B and respiratory syncytial viruses in respiratory specimens호흡기 시편에서 SARS-COV-2, 인플루엔자 A/B 및 호흡기 융기 바이러스의 동시 검출 및 분화를위한 Ampliquick ® 호흡기 삼중 탈수 분석의 분석 성능Research Article Published on 2022-01-052022-09-10 Journal: PLoS ONE [Category] 진단, 치료기술, [키워드] 2019-nCoV assay acute respiratory syndrome acute respiratory syndrome coronavirus analyses assays Chain Reaction comparable Concordance coronavirus cycle threshold Diagnosis diagnostics differentiation evaluated France high correlation Influenza influenza A influenza B Manifestations multiplex observation Panel Patient polymerase chain reaction positive positive sample qualitative detection respiratory Respiratory samples respiratory specimen respiratory specimens respiratory syncytial virus respiratory tract samples respiratory viral infection respiratory viral infections RSV SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specimen Therapeutic approach therapeutic approaches triplex Viral virus viruses [DOI] 10.1371/journal.pone.0262258 PMC 바로가기 [Article Type] Research Article
Evaluation of Three Automated Extraction Systems for the Detection of SARS-CoV-2 from Clinical Respiratory SpecimensArticle Published on 2022-01-042022-10-29 Journal: International Review of Cell and Molecular Biology [Category] COVID-19, [키워드] acid acute respiratory syndrome Affect Analysis automated extraction system Basel cause clinical Concordance contagious coronavirus coronavirus disease COVID-19 COVID-19 patients Culture detect detection DNA exhibited extraction help Inc. infectious dose Limits of detection management median molecular mRT-qPCR NPs nucleic acid extraction platform polymerase chain provided Quantitative rapid increase respiratory respiratory specimen RNA Roche RT-qPCR Saliva SARS-CoV-2 SARS-COV-2 infection sensitivity specificity sputum statistically subject Switzerland System Taiwan TCID Thermo Fisher Scientific tissue Transcription USA [DOI] 10.3390/life12010068 PMC 바로가기 [Article Type] Article
A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2SARS-CoV-2의 N501Y 변이체를 신속하게 식별하기 위한 저비용 TaqMan 작은 홈 결합제 프로브 기반 1단계 RT-qPCR 분석Article Published on 2022-01-012022-09-11 Journal: Journal of virological methods [Category] SARS, 변종, 치료기술, [키워드] Accuracy allele frequency analytical sensitivity B.1.1.7 B.1.351 collected COVID-19 detect determine diagnostic Diagnostic accuracy evaluated expand Frequency Genome Genome sequencing gold gold standard health authorities house identify in some Lineage low frequency Mutation N501Y N501Y mutation N501Y variants One-step RT-qPCR positive Prevalence qPCR qPCR assay reduced reference standard respiratory specimen respiratory specimens robust RT-qPCR SARS-CoV-2 SARS-CoV-2 lineage SARS-CoV-2 variant SARS-CoV-2 variants Sequencing Surveillance TaqMan time-consuming Transmissibility variant variant of concern variants variants of concern variants of SARS-CoV-2 whole-genome sequencing widespread wildtype [DOI] 10.1016/j.jviromet.2021.114333 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare FindingArticle Published on 2021-12-292022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] absence clearance clinical suspicion COVID-19 detection disease finding information molecular diagnostics Nasopharyngeal swab nasopharyngeal swab specimen Patient performed platform positive recovered patient respiratory specimen RT-LAMP RT-qPCR Salamanca SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection Sensitivity and specificity Spain Swab tested Urine viral particle Viral RNA [DOI] 10.3390/jcm11010158 PMC 바로가기 [Article Type] Article
A Novel High-Throughput Nanopore-Sequencing-Based Strategy for Rapid and Automated S-Protein Typing of SARS-CoV-2 VariantsCommunication Published on 2021-12-192022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] Amino acid amplification amplify Analysis approaches automated bioinformatics bottleneck diagnostic flow cell identify Immune escape immunodominant molecular Mutation Nanopore Next-generation sequencing novel offer primer primer pair protocol Rapid reduced region of interest required respiratory specimen restriction S-gene S-protein S-protein variant SARS-CoV-2 SARS-CoV-2 variant sequence sequenced sample Sequencing Strategy subsequent Surveillance typing vaccine escape validation variant variants VoC whole-genome whole-genome sequencing [DOI] 10.3390/v13122548 PMC 바로가기 [Article Type] Communication
Research on the Epidemiology of SARS-CoV-2 in Essential Response Personnel (RECOVER): Protocol for a Multisite Longitudinal Cohort StudyProtocol Published on 2021-12-032022-10-29 Journal: JMIR Research Protocols [Category] COVID-19, [키워드] baseline Care clinical outcomes Cohort cohort study collected COVID-19 COVID-19 pandemic COVID-19 vaccine Critical demographics dose Effectiveness emergency response enrolled Enrollment Essential essential and frontline workers essential worker Essential workers expected First responder hcp Health health care personnel high risk Identifier incidence Infection information initiated longitudinal multisite nasal swab objective observation period participant Participants performed polymerase chain positive Prospective prospective cohort study public health report respiratory specimen responders response Result reverse transcription risk factor Risk factors RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections Surveillance Symptom symptom duration symptomatic and asymptomatic Symptoms vaccination vaccine effectiveness worker [DOI] 10.2196/31574 PMC 바로가기 [Article Type] Protocol
An open-label randomized controlled trial of the effect of lopinavir/ritonavir, lopinavir/ritonavir plus IFN-β-1a and hydroxychloroquine in hospitalized patients with COVID-19코로나19로 입원한 환자를 대상으로 한 로피나비르/리토나비르, 로피나비르/리토나비르 + IFN-β-1a 및 히드록시클로로퀸의 효과에 대한 공개 무작위 대조 시험Clinical Trial Published on 2021-12-012022-09-11 Journal: Clinical microbiology and infection : the official [Category] 임상, 치료제, [키워드] 95% confidence interval 95%CI acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adaptive adjusted odds ratio adults hospitalized age analyses Arm ARMS clinical status conducted Controlled trial coronavirus coronavirus 2019 disease COVID-19 evaluated expected hospitalized patient Hydroxychloroquine Inpatient intention-to-treat population Interferon β-1a IQR Lopinavir LOPINAVIR AND RITONAVIR Lopinavir/ritonavir male median age multicentre no significant effect not improved Occurrence Open-label Ordinal Scale outcome oxygen participant pharmacokinetic plasma concentration Primary outcome quantification Randomized Randomized controlled trial regimen Remdesivir respiratory specimen respiratory specimens respiratory tract specimens Ritonavir SARS-CoV-2 SARS-CoV-2 clearance secondary Secondary outcomes Serious Adverse Event Serious Adverse Events seven-point ordinal scale severe acute respiratory syndrome Coronavirus severe disease significant effect significantly higher solidarity Solidarity trial Standard of care the median the WHO treat Treatment Trial trough ventilatory support Virological with COVID-19 [DOI] 10.1016/j.cmi.2021.05.020 PMC 바로가기 [Article Type] Clinical Trial
Long-term specific IgG response to SARS-CoV-2 nucleocapsid protein in recovered COVID-19 patients회수 된 CovID-19 환자에서 SARS-COV-2 뉴 클레오 캡시드 단백질에 대한 장기 특이 적 IgG 반응Article Published on 2021-12-012022-09-01 Journal: Scientific Reports [Category] SARS, 진단, 치료기술, 치료법, [키워드] absence acute respiratory syndrome acute respiratory syndrome coronavirus age anti-N IgG Blood blood sampling collected coronavirus coronavirus disease Coronavirus Disease (COVID)-19 correlated COVID-19 symptom IgG IgG response immune response immunoassay Immunoglobulin Immunoglobulin G Infection Infectious diseases Microparticle Patient Pneumonia positively correlated presence or absence Protein recovered COVID-19 patient Recovered COVID-19 patients respiratory respiratory specimen respiratory specimens SARS-CoV-2 detection SARS-CoV-2 nucleocapsid Seropositivity serum sample serum samples severe acute respiratory syndrome Coronavirus significantly lower specimens symptom onset Symptoms Vaccine Virology was measured [DOI] 10.1038/s41598-021-02659-4 PMC 바로가기 [Article Type] Article
Meropenem-Resistant Pandoraea Pneumonia in a Critically Ill Patient With COVID-19Infectious Disease Published on 2021-11-122022-10-31 Journal: Cureus [Category] COVID-19, [키워드] 16s ribosomal dna sequencing acute respiratory distress antibiotic Antibiotic resistance ARDS Bacterial carbapenem carbapenems Care Co-infection COVID-19 Critical care Critically ill Culture drug resistance fibrosis Ill IMPROVE In-vitro increasingly Infection initiated Laboratory testing mechanical ventilation multi-drug resistant multi-organ failure P. aeruginosa pandoraea species Patient Pneumonia pneumoniae proinflammatory reduce regimen reported respiratory specimen respiratory viruses rising SARS-COV-2 infection secondary bacterial pneumonia sensitivity sputum culture subsequent susceptibility syndrome transitioned Treatment VAP Ventilator associated pneumonia with COVID-19 [DOI] 10.7759/cureus.19498 PMC 바로가기 [Article Type] Infectious Disease
Coronavirus Disease 2019 (COVID-19) Re-infection by a Phylogenetically Distinct Severe Acute Respiratory Syndrome Coronavirus 2 Strain Confirmed by Whole Genome Sequencing코로나바이러스 질병 2019(COVID-19) 계통발생학적으로 뚜렷한 중증 급성 호흡기 증후군에 의한 재감염 전체 게놈 시퀀싱으로 확인된 코로나바이러스 2 균주Article Published on 2021-11-022022-09-12 Journal: Clinical Infectious Diseases: An Official Publicat [Category] MERS, SARS, 진단, [키워드] acute infection Amino acid amino acids Analysis analyzed asymptomatic infection C-reactive protein collected Comparative conducted Confirmed by coronavirus 2 COVID-19 D614G elevated Evidence Genome genomic GISAID Human IgG Immunity immunological Infection natural infection nucleotide occur occurred ORF8 Patient phylogenetically Protective Proteins Re-infection respiratory respiratory specimen RT-PCR Ct SARS-CoV-2 SARS-CoV-2 IgG Sequencing Seroconversion serological serum stop codon strain symptomatic T cell epitopes the patient Vaccine design viral genome viral shedding virus virus genome was performed whole genome Whole genome sequencing [DOI] 10.1093/cid/ciaa1275 PMC 바로가기 [Article Type] Article