Strategies for Vaccination: Conventional Vaccine Approaches Versus New-Generation Strategies in Combination with AdjuvantsReview Published on 2021-01-222022-10-28 Journal: Pharmaceutics [Category] COVID-19, [키워드] absence adjuvant adjuvants adopted Antigen Antiviral treatment applied approach caused Combination Conventional COVID-19 pandemic develop disadvantage DNA vaccine effective Effective vaccines ENhance expressed Genetic group inactivated initial mRNA mRNA stability nanoparticle outbreak pathogen peptides Protein Proteins Psychological raised Research retroviral RNA RNA vaccine SARS-CoV-2 Spread Strategy suppress transfer Vaccine vaccine adjuvant Vaccine development Viral vector virulent virus [DOI] 10.3390/pharmaceutics13020140 PMC 바로가기 [Article Type] Review
SARS-CoV-2 Spike Alterations Enhance Pseudoparticle Titers and Replication-Competent VSV-SARS-CoV-2 VirusArticle Published on 2020-12-182022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] acute respiratory syndrome alteration BSL2 can be used caused Cell cell tropism conditions coronavirus cytoplasmic tail death demonstrated determine expression fusion global pandemic IMPROVE Modification Mutation Particle Particles produced pseudotyped recombinant VSV removal residue retroviral SARS-CoV-2 spike spike glycoprotein the S protein titer variant vesicular stomatitis virus virus VSV while [DOI] 10.3390/v12121465 PMC 바로가기 [Article Type] Article
Droplet Microarray Based on Nanosensing Probe Patterns for Simultaneous Detection of Multiple HIV Retroviral Nucleic Acids여러 HIV 레트로바이러스 핵산의 동시 검출을 위한 나노센싱 프로브 패턴을 기반으로 하는 액적 마이크로어레이Article Published on 2020-12-162022-09-11 Journal: ACS applied materials & interfaces [Category] SARS, 치료기술, 치료제, [키워드] 2D MoS2 nanosheets acid Adsorption demonstrated dendrimer detection droplet droplet microarray (DMA) energy transfer FIVE fluorescent fluorescent dye fluorescent resonance energy transfer (FRET) HIV HIV nucleic acids HIV-1 HIV-2 Human immunodeficiency virus human immunodeficiency virus-2 hybridized immunodeficiency Influenza influenza A Influenza A) limit of detection M gene Microarray molybdenum Multiple multiplex N gene N genes nucleic acid nucleic acids omniphobic. pattern platform probe proof proof of concept retroviral separated Simultaneous small sample size Viral viral gene viral genes viral infection viral nucleic acid viral nucleic acids weakening [DOI] 10.1021/acsami.0c16146 PMC 바로가기 [Article Type] Article
Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoproteinArticle Published on 2020-11-052022-10-29 Journal: Scientific Reports [Category] COVID-19, SARS, [키워드] 293T biosafety convalescent patient Efficiency expressing full-length G glycoprotein glycoprotein hACE2 highly pathogenic IgG levels lentiviral lentivirus mammalian cell mammalian cells Microbiology much higher neutralized Particle Patient Preclinical research produced provide pseudotype pseudotyped Pseudotyped virus recovered COVID-19 patient retroviral SARS-CoV-2 SARS-CoV-2 neutralizing antibody SARS-CoV-2 receptor SARS-CoV-2 spike glycoprotein SEROLOGICAL STUDY serum specific IgM specific neutralizing antibody spike tested the SARS-CoV-2 transduced vesicular stomatitis virus Virology virus VSV-G while [DOI] 10.1038/s41598-020-76135-w PMC 바로가기 [Article Type] Article
Anti-spike, Anti-nucleocapsid and Neutralizing Antibodies in SARS-CoV-2 Inpatients and Asymptomatic IndividualsMicrobiology Published on 2020-10-192022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] anti-N antibody anti-RBD anti-S2 anti-SARS-CoV-2 anti-SARS-CoV-2 antibody antibody asymptomatic individual asymptomatic patients Convalescent plasma therapy COVID-19 declined detectable evaluate help immune response in-house ELISAs individual induce infected patients Inpatient majority Neutralizing antibodies neutralizing antibody Neutralizing antibody response nucleocapsid Particle post-symptom onset predict pseudotyped RBD reached Reinfection retroviral SARS-CoV-2 Seroconversion serological Serological assay seropositive shown specific antibody spike subunits the SARS-CoV-2 undetectable Vaccine Vaccines [DOI] 10.3389/fmicb.2020.584251 PMC 바로가기 [Article Type] Microbiology
Medical research during the COVID-19 pandemicCOVID-19 전염병 동안 의료 연구Review Published on 2020-08-062022-09-11 Journal: World J Clin Cases [Category] 비임상, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 acute respiratory syndrome coronavirus-2 affected approach best breath Care caused clinical clinical experience clinical trial Clinical trial design clinical trials collaboration Complete conduction Contact contact with contagiousness coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus-2 country COVID-19 COVID-19 pandemic Critical deaths experimental animal models flexible Follow-up Health Health care hospitals identify implementation in vitro include infections initiated involved Laboratory long term Medical research medical staff medication mental health Modification modifications MOST Novel coronavirus number of deaths pandemic participant Patient physical positive patients professional reason reported Research respiratory retroviral rights running sample sizes SARS-CoV-1 SARS-CoV-2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus specific treatment spread to suggested survivor therapy today Treatment Trial vaccination virus viruses with COVID-19 Wuhan Wuhan, China [DOI] 10.12998/wjcc.v8.i15.3156 PMC 바로가기 [Article Type] Review
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization AssaysProtocol Published on 2020-05-062022-10-28 Journal: Viruses [Category] COVID-19, [키워드] 293T cell 293T-ACE2 ACE2 Affect ALAYT antibody Antibody binding assays ATCC BEI Resource Cell complement coronavirus COVID-19 cytoplasmic tail demonstrated described Express human sera Immunity infect lentiviral Lentiviral particle lentiviral pseudotype luciferase neutralization Neutralization assay Neutralizing activity Neutralizing antibodies NIH Particle Particles plasma Protein protocol pseudotype pseudotyped lentiviral particle pseudotyping reagent receptor Repository retroviral SARS-CoV-2 sera spike the SARS-CoV-2 vesicular stomatitis virus viral infection viral particle virus VSV [DOI] 10.3390/v12050513 PMC 바로가기 [Article Type] Protocol
Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID miceOriginal Article Published on 2015-09-082022-10-28 Journal: Archives of Virology [Category] Coronavirus, MERS, [키워드] absence association clone combined immunodeficiency comparison coronavirus determinant Driver cDNA Frame genome sequence genomic sequence Hepatitis identify Langerhans cell histiocytosis liver mice murine murine hepatitis virus new strain new virus nucleotide sequence PCR predicted Protein protein level retroviral retrovirus SCID Mouse sequence sequenced Subtract cDNA Library suppression Suppression Subtractive Hybridization Tester cDNA tissues unique virulent virus virus strain was used whole genome [DOI] 10.1007/s00705-015-2592-y PMC 바로가기 [Article Type] Original Article
Human Genetics in Rheumatoid Arthritis Guides a High-Throughput Drug Screen of the CD40 Signaling Pathway류마티스 관절염의 인간 유전학은 CD40 신호 전달 경로의 고처리량 약물 검사를 안내합니다Research Article Published on 2013-05-162022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activated After allele Analysis approved drug Arthritis association Author B cells B lymphocyte B lymphocytes Biology can be used CD19+ CD40 CD40 ligand CD40 signaling Cell cell line chemical compounds clinical care coding Combination complex Compound consequence controls correlation counter deep sequencing develop disease drug Drug discovery drugs effort eQTL ExoN exonic explain expression finding Follow-up gain-of-function Genetic genetic findings Genome-wide association studies Genome-wide association study genotyping guide GWAS healthy control homozygous Human Human genetics identify implicated increase individuals Infection Inflammation inhibit inhibitor loci locus lymphocyte Lymphocytes mononuclear cell mononuclear cells mouse NF-kB NF-κB NF-κB signaling non-risk allele observé p65 pathway Patient Peripheral blood Peripheral blood mononuclear cells Phosphorylation Protein Quantitative RA RELA reporter retroviral Rheumatoid rheumatoid arthritis risk risk allele Screen second Sequencing seropositive Signaling single SNP small molecule Small molecules small-molecule screen subject subunit therapy trait traits transcription factor treat variant while [DOI] 10.1371/journal.pgen.1003487 PMC 바로가기 [Article Type] Research Article
Identification of a Genomic Reservoir for New TRIM Genes in Primate Genomes영장류 게놈에서 새로운 TRIM 유전자에 대한 게놈 저장소 식별Research Article Published on 2011-12-012022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] African antiviral gene antiviral genes Author best Biology characterized chromosome chromosome 11 Cluster compared copy number Defense described determine Divergence duplication duplications Evolution expressed found Gene gene cluster Genes Genome genomes Han Chinese HIV Human Human genome human population identification immune system individual innate immune response innate immune system Innate immunity locus majority mechanism MOST natural selection neutralized New pathogen Pathogens positive primate primates question Region reservoir restrict restriction factor retroviral rhesus macaques screened selected Specificities target specificity Tripartite Motif ubiquitin ligase virus recognition viruses young gene [DOI] 10.1371/journal.pgen.1002388 PMC 바로가기 [Article Type] Research Article