Evaluating Antibody Mediated Protection against Alpha, Beta, and Delta SARS-CoV-2 Variants of Concern in K18-hACE2 Transgenic MiceK18-hACE2 형질전환 마우스에서 우려되는 알파, 베타 및 델타 SARS-CoV-2 변이체에 대한 항체 매개 보호 평가Article Published on 2022-03-232022-09-11 Journal: Journal of Virology [Category] MERS, SARS, 변종, 진단, 치료법, [키워드] Alpha Alpha VOC angiotensin angiotensin converting enzyme Beta Brain can be used concern convalescent convalescent plasma COVID-19 COVID-19 pandemic COVID-19 patient Critically ill decrease Delta Delta VOC disease dose Efficacy elevated enzyme evaluate Evaluating hACE2 hcp Human IgG titer immune evasion immunization in vivo model K18 K18-hACE2 K18-hACE2 transgenic mice lethality lung Mediated mice modeling modeling COVID-19 monoclonal antibody Mortality not decreased nucleocapsid nucleocapsid IgG pandemic passive immunity passive immunization polyclonal antibodies polyclonal antibody problem protection RBD Receptor binding domain response SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variants serum single dose Strains survival These data transgenic Transgenic mouse Treatment treatment group Vaccine variants variants of concern Viral Viral RNA while [DOI] 10.1128/jvi.02184-21 PMC 바로가기 [Article Type] Article
SARS-CoV-2 variants of concern display enhanced intrinsic pathogenic properties and expanded organ tropism in mouse models우려되는 SARS-CoV-2 변이체는 마우스 모델에서 향상된 고유 병원성 특성 및 확장된 기관 친화성을 나타냅니다.Article Published on 2022-02-152022-09-11 Journal: Cell Reports [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] accelerated ACE2 receptor Alpha Alpha variant Antibody neutralization Beta Beta variant cellular cellular immunity curtail cytokine cytokine production Depletion disease Disease progression elicit expand Gamma Gamma variant gastrointestinal tract hACE2 host range human ACE2 immune activation immune responses induce Infection Inflammation innate immune response interferon intrinsic ISG ISG expression K18-hACE2 lung Lungs management mice mouse model murine Natural killer cells neutralization organ pandemic pathogenic pathogenicity reduced replicate responses reveal SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants Spread Symptoms systemic infection tissue transgenic Transmissibility Trigger variants variants of concern VoC VOCs wild-type zoonotic transmission [DOI] 10.1016/j.celrep.2022.110387 PMC 바로가기 [Article Type] Article
Immunodominant Linear B-Cell Epitopes of SARS-CoV-2 Spike, Identified by Sera from K18-hACE2 Mice Infected with the WT or Variant VirusesArticle Published on 2022-02-072022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] Analysis anti-viral antibody approach B-cell epitope B.1.1.7 B.1.351 Beta collected contribute COVID-19 COVID-19 vaccine cross-protective diagnostic Efficacy epitope epitope mapping examined Frequency Genetic variant host cell humoral Identified immune immunodominant immunogenic immunological Immunotherapy implication in vitro experiment Infection K18-hACE2 linear epitope linear epitopes mice neutralization tests SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 strain SARS-CoV-2 variants serum sample serum samples spike Spike protein targets the SARS-CoV-2 the spike protein transgenic viral entry virus variant [DOI] 10.3390/vaccines10020251 PMC 바로가기 [Article Type] Article
SARS-CoV-2 spike S1 subunit induces neuroinflammatory, microglial and behavioral sickness responses: Evidence of PAMP-like propertiesSARS-CoV-2 스파이크 S1 소단위체는 신경염증, 소교세포 및 행동병 반응을 유도합니다: PAMP 유사 특성의 증거Article Published on 2022-02-012022-09-11 Journal: Brain, behavior, and immunity [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] activated Activation Anxiety avoidance Brain brain region brain regions CAGE CD11b CD200R1 cells characterized CNS cognitive CXCL1 cytokine signaling demonstrated Depression Direct direct viral infection Evidence evidence of examined function Gene Expression GFAp HEK293 HEK293 cells HMGB1 Hypothesis IFNγ IL-10 IL-1β IL-2 IL-6 IL1B in vitro include independent independent of induce Infection Inflammatory injected male mechanism MHCII microglia microglial modulated Neuroinflammation neurological Neuropsychiatric NLRP3 PAMP PAMPs pathogen pathogen-associated molecular Pattern recognition pattern recognition receptor Protein protein level protein levels receptor signaling reduced S1 subunit SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike protein Sickness Sickness behavior spike Spike protein structural protein structural proteins Symptom the S1 subunit the SARS-CoV-2 TLR TLR. TLR2 TLR4 TNF transgenic Treatment vehicle viral infection [DOI] 10.1016/j.bbi.2021.12.007 PMC 바로가기 [Article Type] Article
A Single Dose of an MVA Vaccine Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Protein Neutralizes Variants of Concern and Protects Mice From a Lethal SARS-CoV-2 Infection사전 융합 안정화 SARS-CoV-2 스파이크 단백질을 발현하는 MVA 백신의 단일 용량은 우려의 변종을 중화하고 치명적인 SARS-CoV-2 감염으로부터 마우스를 보호합니다Immunology Published on 2022-01-272022-09-11 Journal: Frontiers in Immunology [Category] 변종, 진단, [키워드] administration animal models Antibody titers C57BL/6 caused Cell clinical trials concern COVID-19 vaccine Cytokines demonstrated dose Efficacy expressed expressing expression full-length hACE2 histopathological IgG immune responses immunized immunogenicity K18-hACE2 mice lesions lethal Lungs mice modified vaccinia virus morbidity morbidity and mortality mouse model MVA vector Neutralizing antibody titer neutralizing antibody titers parental poxvirus prefusion-stabilized S protein pro-inflammatory cytokine pro-inflammatory cytokines protective efficacy Protein reducing S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 S protein SARS-CoV-2 spike SARS-CoV-2 vaccine SARS-CoV-2 viral loads single dose spike T-cell immune responses transgenic vaccine candidate variants variants of concern Viral viral loads viruses wild-type [DOI] 10.3389/fimmu.2021.824728 PMC 바로가기 [Article Type] Immunology
Plant-Based Vaccines in Combat against Coronavirus DiseasesReview Published on 2022-01-182022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] Administered antigens applied biopharming caused combat Coronaviruses CoV COVID-19 COVID-19 pandemic Diseases DNA and RNA evaluated expression glycosylated immunodominant epitope immunogenicity inactivated viruses indicate injection membrane MERS MERS-CoV Middle East mucosa mucosal nasal Oral vaccine plant plant-based vaccines plants processed produced Protein Proteins purification purified Research respiratory Respiratory system SARS-CoV SARS-CoV-2 subunit tissue transgenic transient expression Vaccine Vaccines variant Viral vector [DOI] 10.3390/vaccines10020138 PMC 바로가기 [Article Type] Review
Animal Models of COVID-19: Transgenic Mouse ModelArticle Published on 2022-01-012022-11-15 Journal: Methods in molecular biology (Clifton, N.J.) [Category] SARS, 변종, 진단, [키워드] acute respiratory syndrome animal animal model Antiviral Brain C57BL/6 cause caused changes in concern coronavirus coronavirus disease COVID-19 COVID-19 disease cytokeratin 18 promoter death described ELISA enzyme-linked immunosorbent evaluated expressing global public health hACE2 hallmark Histopathology human Angiotensin-converting enzyme IHC immune response immunohistochemistry infected intranasally Infection K18 K18 hACE2 transgenic mice lung Lungs Model morbidity Mortality mouse mouse model nasal nervous system neutralizing monoclonal antibodies pathogenesis of SARS-CoV-2 pathogenicity pathology Prophylactic protocol respiratory tracts responses SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 strain spleen Spread survival the cytokine storm therapeutic tissues transgenic transgenic mice Treatment Vaccine viral pathogenesis viral replication virus Wuhan, China [DOI] 10.1007/978-1-0716-2111-0_16 PMC 바로가기
A promiscuous T cell epitope-based HIV vaccine providing redundant population coverage of the HLA class II elicits broad, polyfunctional T cell responses in nonhuman primatesResearch article Published on 2022-01-012022-10-05 Journal: Vaccine [Category] 비임상, [키워드] alleles Antigen approach BALB/c binding CD4+ CD4+ T cell CD8+ T cell cellular immunity conserved costly Coverage COVID-19 described Diseases DNA vaccine Electroporation elicit elicited ENCODE epitope Epitope-based vaccine Epitopes Evidence facilitate generate HIV HIV vaccine HLA class II HLA-DR immune response immunization immunized immunodominant immunogen in vivo Increasing induce less MHC mice multiple epitopes Multiple HLA-binding epitopes neutralizing antibody over patients peptides platform Populational vaccine response Result rhesus macaque rhesus macaques shown T cell T cell epitope T cell response transgenic Vaccine Vaccines viral disease viral infections [DOI] 10.1016/j.vaccine.2021.11.076 [Article Type] Research article
Iota-Carrageenan Inhibits Replication of SARS-CoV-2 and the Respective Variants of Concern Alpha, Beta, Gamma and DeltaIota-Carrageenan은 SARS-CoV-2 및 우려되는 알파, 베타, 감마 및 델타의 각 변종 복제를 억제합니다Article Published on 2021-12-082022-09-10 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 치료제, [키워드] age Alpha antiviral activity Antiviral effect Beta Calu Calu-3 can not carrageenan carrageenan types Carrageenans comparable concern contribute coronavirus COVID-19 COVID-19 pandemic Defense Delta effective exhibit Gamma human lung cell human lung cells independent independent of infect Infection inhibit investigated Iota iota-carrageenan kappa-carrageenan lack Lambda lambda-carrageenan lentivirus nasal Particle Prophylaxis pseudotyped pseudotyping public health threat reduce SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 spike Spread SSPL sulfated polymer sulfated polysaccharide sulfated polysaccharides supply the SARS-CoV-2 TMPRSS2 transgenic transmission of SARS-CoV-2 transmitted Treatment vaccination Vaccine Vaccine-induced immunity variants variants of concern Viral Viral transmission virulent virus variants VoC VOCs Wuhan [DOI] 10.3390/ijms222413202 PMC 바로가기 [Article Type] Article
Postmortem Stability of SARS-CoV-2 in Mouse Lung TissueResearch Letter Published on 2021-12-012022-10-31 Journal: Emerging Infectious Diseases [Category] COVID-19, SARS, [키워드] 2019 novel coronavirus disease acute respiratory syndrome Antigen coronavirus coronavirus disease Coronaviruses COVID-19 death Deceased declined determine Experimental infection hACE2 Host Infectious virus K18 K18 hACE2 mice lung mice mouse organism Postmortem respiratory infections RNA copy RT-PCR SARS-CoV-2 severe acute respiratory syndrome coronavirus 2 TCID50 transgenic virus infectivity viruses zoonoses [DOI] 10.3201/eid2712.211621 PMC 바로가기 [Article Type] Research Letter