SARS-CoV-2 Seroprevalence Study in Pediatric Patients and Health Care Workers Using Multiplex Antibody ImmunoassaysArticle Published on 2022-09-142022-11-15 Journal: Viruses [Category] SARS, 변종, 진단, 치료기술, [키워드] Adults affected antibody Antigen Beta children circulating Clinical characteristics Cohort correlation COVID-19 delta variant disease ELISA estimate exacerbated False positive Gamma group HCW Health healthcare healthcare worker Healthcare workers highlight hospitals IgA IgA response IgG IgM indicated Infection isotype lowest Luminex assay Mild nucleocapsid PIMS-TS positive correlation RBD recruited SARS-CoV-2 SARS-CoV-2 proteins sensitivity Seropositivity Seroprevalence significantly lower Spain Spanish specificity study period the receptor-binding domain variants were measured worker [DOI] 10.3390/v14092039 PMC 바로가기
B-Cell Responses in Hospitalized Severe Acute Respiratory Syndrome Coronavirus 2-Infected Children With and Without Multisystem Inflammatory SyndromeArticle Published on 2022-09-132022-11-15 Journal: The Journal of Infectious Diseases [Category] SARS, 진단, [키워드] acute respiratory syndrome antibodies antibody Antibody avidity Antibody Response antibody-secreting cell antibody-secreting cells Antiviral antiviral antibody B cell B cells B-cell response children circulation complicate coronavirus coronavirus disease COVID-19 COVID-19 patient demonstrated flow cytometry Follow-up group Hospitalized IgG immune response Immunoglobulin Infection Inflammatory MIS-C phenotype plasma Postinfectious proportion respiratory response SARS-CoV-2 sustained syndrome those with MIS-C with COVID-19 [DOI] 10.1093/infdis/jiac119 PMC 바로가기
Durable antibody and effector memory T cell responses in breastmilk from women with SARS-CoV-2Article Published on 2022-09-122022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, [키워드] antibody asked B.1.1.7 B.1.351 Beta binding Blood breast milk breast permeability breastmilk CCR7 CD4 CD45RO CD8 T cell Cell complex COVID-19 COVID-19 vaccine D614G domain dried blood spot effector memory elect enrolled expressed first COVID-19 symptom flow cytometry Gamma IgA IgA response IgG IgM Immunity Immunoglobulin indicated Infant lactating memory memory T memory T cells milk mother mucosal mucosal immunity neutralize neutralized Neutralizing P.1 participant passive immunity permeability positive potassium ion pregnant women Protective reactivity receive robust SARS-CoV-2 SARS-COV-2 infection second set significantly higher sodium Stool T cell T cell response tested the spike protein timepoint was collected were assessed women [DOI] 10.3389/fimmu.2022.985226 PMC 바로가기
Intranasal administration of a single dose of MVA-based vaccine candidates against COVID-19 induced local and systemic immune responses and protects mice from a lethal SARS-CoV-2 infectionArticle Published on 2022-09-122022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, 치료기술, [키워드] absence acute respiratory syndrome Administered administration animal model blocked bronchoalveolar C57BL/6 caused CD4 CD8 coronavirus Coronavirus disease-19 COVID-19 COVID-19 progression current demonstrated effective Efficacy elicited evaluated expressing full-length histopathological IgA antibody IgG immune responses Immunity immunogenic immunogenicity induce intramuscular intranasal intranasal delivery K18-hACE2 transgenic mice Local lung lung lesion lymph nodes mice modified vaccinia virus morbidity and mortality mucosal mucosal immune MVA nasal washe Nasal washes neutralizing antibody parental Prevent pro-inflammatory cytokine PROTECT Protective protective efficacy Protein responses Result S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 transmission SARS-CoV-2 variant SARS-CoV-2 virus SARS-CoV-2-specific immune response serum single dose spleen susceptible systemic immune response systemically T-cell immune response the SARS-CoV-2 Transmission undetectable upper respiratory tract vaccination Vaccine vaccine administration vaccine candidate vaccine candidates vaccine dose virus infection [DOI] 10.3389/fimmu.2022.995235 PMC 바로가기
Arsenal of nanobodies shows broad-spectrum neutralization against SARS-CoV-2 variants of concern in vitro and in vivo in hamster modelsArticle Published on 2022-09-092022-11-15 Journal: Communications Biology [Category] SARS, 변종, 진단, 치료기술, [키워드] anti-RBD anti-S2 anti-SARS-CoV-2 antibody fragment arsenal candidate characterized conserved cross-reactivity demonstrated detectable Efficacy epitope feasibility IgG in vitro in vivo intrinsic mAb magnitude nanobodies nanobody neutralization offer provide reducing SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant SARS-CoV-2 variants shown specificity spike glycoprotein therapeutic thermodynamic viral burden [DOI] 10.1038/s42003-022-03866-z PMC 바로가기
Sialic Acid and Fucose Residues on the SARS-CoV-2 Receptor-Binding Domain Modulate IgG Antibody ReactivityArticle Published on 2022-09-092022-11-15 Journal: ACS Infectious Diseases [Category] SARS, 진단, [키워드] acid antibody antibody reactivity carbohydrate cells Chinese hamster conserved defined domain embryonic kidney cell ENhance evaluated exhibited fucose generate glycoepitope glycoproteomics IgG less mammalian cells mass spectrometry modulated monosaccharides N-linked glycans Neutralizing antibodies produced Protein RBD reactivity Receptor binding domain reduced removal SARS-CoV-2 SARS-CoV-2 positive patients serum sample sialic acid Spike protein Structure the RBD the RBD protein the SARS-CoV-2 vaccine candidates Viral proteins [DOI] 10.1021/acsinfecdis.2c00155 PMC 바로가기
Differences in systemic and mucosal SARS-CoV-2 antibody prevalence in a prospective cohort of Dutch childrenArticle Published on 2022-09-092022-11-15 Journal: Frontiers in Immunology [Category] SARS, 진단, [키워드] 95% CI affected antibodies antibody antibody assay Antibody assays Antibody prevalence association children clinical variables cohort study Concordance cross-sectional Demographic variables difference evaluate healthy children Humoral immunity IgA IgG IgG positivity Immunity Immunocompromised infected children Infection investigated medical service MONITOR mucosal mucosal antibody response mucosal IgG Netherland Non-invasive performed positive Prevalence prospective cohort RBD RBD-specific IgG Receptor binding domain reduced Saliva saliva antibodies. SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 spike serum Sex Symptom vaccination [DOI] 10.3389/fimmu.2022.976382 PMC 바로가기
Humoral response to different SARS-CoV-2 vaccines in orthotopic liver transplant recipientsArticle Published on 2022-09-092022-11-15 Journal: Vaccine [Category] SARS, 진단, [키워드] Abbott Diagnostics Ad5-nCoV Analysis analyzed applied blood sample BNT162b2 BNT162b2 vaccine ChAdOx1 China Comorbidity Complete Control controls CoronaVac COVID-19 COVID-19 vaccine DiaSorin Efficacy evaluate Gam-COVID-Vac healthy healthy controls humoral Humoral response IgG immunosuppressants Italy life liver median Mexico mRNA-1273 multivariable model Oxford-AstraZeneca Pandemia participant Patient patients Pfizer-BioNTech platform positive Prednisone recipient recipients reduced SARS-CoV-2 vaccine Serologic response serological response Sinovac USA vaccination Vaccine vaccine doses Vaccines women [DOI] 10.1016/j.vaccine.2022.08.027 PMC 바로가기
mRNA vaccination drives differential mucosal neutralizing antibody profiles in naïve and SARS-CoV-2 previously-infected individualsArticle Published on 2022-09-082022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, 치료기술, [키워드] 95% CI ACE2 ACE2 Inhibition addition antibody Antibody responses Beta coronaviruses binding BNT162b2 vaccine Breakthrough infection characterized contribute cross-reactive Defense dose healthcare worker HKU1 human coronavirus humoral IgA IgA response IgG IgG level IgG response immunoassay individual individuals induce infected with SARS-CoV-2 investigated less Meso Scale Discovery mRNA mRNA vaccination mRNA vaccine mucosal mucosal immune mucosal immunity multiplex naïve neutralizing antibody neutralizing response neutralizing responses neutralizing responses. OC43 of BNT162b2 participant Participants pathogenic plasma positively correlated profile response SARS-CoV-2 SARS-CoV-2 IgG SARS-COV-2 infection SARS-CoV-2-specific IgG second dose selected sensitivity Spearman spike-specific IgG Transmission vaccination vaccine dose variants of concern [DOI] 10.3389/fimmu.2022.953949 PMC 바로가기
Recombinant COVID-19 vaccine based on recombinant RBD/Nucleoprotein and saponin adjuvant induces long-lasting neutralizing antibodies and cellular immunityArticle Published on 2022-09-082022-11-15 Journal: Frontiers in Immunology [Category] SARS, 진단, 치료기술, [키워드] caused CD4+ CD4+ and CD8+ CD8+ T cell Cell cellular immunity Combination COVID-19 vaccine cytokine domain doses E. coli effective elicit elicited ELISA expressed female flow cytometry global pandemic granzyme groups IFN-γ IgG IgG1 IgG2a IL-12 IL-4 immune response immunized increase in induce infecting isotype long-lasting long-lasting neutralizing antibody lymphoproliferative mice N protein neutralization neutralization antibodies Neutralization antibody nucleocapsid percentage promoted Protective purified purified RBD quantified RBD recombinant Recombinant protein responses robust Saponin SARS-CoV-2 SARS-CoV-2 vaccine significantly spleen subcutaneously surrogate sVNT T cell response Test the SARS-CoV-2 Vaccine vaccine candidates vaccine group virus were measured [DOI] 10.3389/fimmu.2022.974364 PMC 바로가기