Efficacy of GC-376 against SARS-CoV-2 virus infection in the K18 hACE2 transgenic mouse modelK18 hACE2 형질전환 마우스 모델에서 SARS-CoV-2 바이러스 감염에 대한 GC-376의 효능Article Published on 2021-05-052022-09-10 Journal: Scientific Reports [Category] 신약개발, 치료제, [키워드] acute respiratory syndrome coronavirus antiviral activity antiviral efficacy antiviral therapies antiviral therapy Brain caused clinical symptom Clinical symptoms Control controls COVID-19 pandemic dose evaluated hACE2 health emergency in vitro in vivo Inflammation inhibitor K18 K18-hACE2 K18-hACE2 mice lead M pro mice Microbiology MOST not improved outcome reduced reduced viral loads reduction in respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus infection Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 Support survival tissue lesions toxic Transgenic mouse treat treated Treatment Viral Viral antigen viral loads viral titer viral titers Virology virus [DOI] 10.1038/s41598-021-89013-w PMC 바로가기 [Article Type] Article
Molecular Docking and Dynamics Simulation Revealed the Potential Inhibitory Activity of ACEIs Against SARS-CoV-2 Targeting the h ACE2 ReceptorChemistry Published on 2021-05-042022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 ACEi ACEIs activity Against Alacepril approach approved drugs assumed binding affinity binding free energy carried clinical trial complexes computational approach concerning coronavirus coronavirus 2 COVID-19 docking result docking study Drug discovery Dynamics effort example exhibiting experiment facilitate hACE2 Health highlight Host human Angiotensin-converting enzyme Hydrogen bond illness in-silico indications information inhibitor Interaction molecular molecular docking molecular dynamics Potential produced provided residues respiratory Revealed SARS-CoV-2 SARS-CoV-2 spike protein Simulation Spread target targeting therapeutic drug thermodynamic these compounds treat Treatment urgency [DOI] 10.3389/fchem.2021.661230 PMC 바로가기 [Article Type] Chemistry
Comprehensive characterization of N- and O- glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2SARS-CoV-2 인간 수용체 안지오텐신 전환 효소 2의 N- 및 O- 글리코실화에 대한 포괄적인 특성화Article Published on 2021-05-032022-09-11 Journal: Glycobiology [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] ACE2 ACE2 glycosylation ACE2 site mapping acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition age Analysis angiotensin angiotensin converting enzyme assist bind binding bioinformatics caused characterized Complication Comprehensive condition conducted coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patients COVID-19 receptor enzyme epitope Epitopes expressed feature GlcNAc glycopeptide glycosylation glycosylation site hACE2 hACE2 N-glycosylation. hACE2 receptor HEK293 cells human angiotensin converting enzyme 2 in viral Interaction Interpretation involved membrane fusion mode modeling N-glycosylation N-glycosylation site O-glycosylation site pandemic pattern Prevent Protein receptor Replication S protein SARS-CoV-2 SARS-CoV-2 receptor Seven severe acute respiratory syndrome Coronavirus Sex sialic acid Therapeutic strategies trimeric spike understanding viral entry [DOI] 10.1093/glycob/cwaa101 PMC 바로가기 [Article Type] Article
A novel pseudovirus‐based mouse model of SARS-CoV-2 infection to test COVID-19 interventionsCOVID-19 개입을 테스트하기 위한 SARS-CoV-2 감염의 새로운 슈도바이러스 기반 마우스 모델Research Published on 2021-04-302022-09-10 Journal: Journal of Biomedical Science [Category] MERS, SARS, 변종, 진단, [키워드] ACE2 addition Adenovirus administration angiotensin animal model binding can be used cause characterized co-transfection coronavirus coronavirus disease Coronavirus disease 2019 Coronaviruses COVID-19 COVID-19 pandemic demonstrated followed by Gag generate hACE2 HIV human ACE2 human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 human cell human cells infect Infection Intervention intranasal lentivirus lentiviruses luciferase-expressing mice non-infectious Outbreaks Plasmid Prevent pseudovirus purified Research respiratory Result SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 pseudovirus SARS-CoV-2 spike protein simple Spike protein Spread Strains supplementary material the SARS-CoV-2 transfection variant virulent virus worldwide pandemic [DOI] 10.1186/s12929-021-00729-3 PMC 바로가기 [Article Type] Research
SARS-CoV-2 501Y.V2 variants lack higher infectivity but do have immune escapeSARS-CoV-2 501Y.V2 변이체는 더 높은 감염성은 없지만 면역 탈출은 있습니다Article Published on 2021-04-292022-09-11 Journal: Cell [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] 501Y.V2 Abstract caused Cell cell type cell types cells convalescent convalescent patient Convalescent patients dominant E484K Efficacy escaped except for exhibit experiment hACE2 higher infectivity Immune escape immunized immunized mice increase in individuals Infectivity inhibitory effect Inhibitory effects K417N lack mACE2 mice monoclonal antibodies monoclonal antibody multiple mutation multiple mutations murine Mutation N501Y N501Y mutation neutralization neutralization ability neutralizing antibody neutralizing monoclonal antibodies neutralizing monoclonal antibody polyclonal antibodies polyclonal antibody Pseudotyped virus Pseudotyped viruses receptor binding region Receptor-binding domain reduced SARS-CoV-2 sera significantly South Africa Spike protein susceptibility the receptor-binding domain Vaccines variant variant of SARS-CoV-2 variants variants of SARS-CoV-2 Viral viral spike protein viruses [DOI] 10.1016/j.cell.2021.02.042 PMC 바로가기 [Article Type] Article
Mutation Signatures and In Silico Docking of Novel SARS-CoV-2 Variants of ConcernArticle Published on 2021-04-262022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] acute respiratory syndrome African Analysis assumed binding binding free energy China clade concern coronavirus D614G demonstrated E484K favor genomic greater hACE2 highest binding affinity human Angiotensin-converting enzyme immune system in silico Approach include independent molecular molecular interaction MONITOR Mutation N501Y nonsynonymous mutation novel pathway provide public health RBD reduce reported residue SARS-CoV-2 SARS-CoV-2 variant selective several variant shown signature South stable complex Surveillance the binding affinity the receptor-binding domain Transmissibility vaccination variant variants variants of concern VoC [DOI] 10.3390/microorganisms9050926 PMC 바로가기 [Article Type] Article
Infectious Clones Produce SARS-CoV-2 That Causes Severe Pulmonary Disease in Infected K18-Human ACE2 Mice감염성 클론은 감염된 K18-인간 ACE2 마우스에서 심각한 폐 질환을 일으키는 SARS-CoV-2를 생성합니다Research Article Published on 2021-04-202022-09-10 Journal: mBio [Category] MERS, SARS, 치료기술, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 benefit Brain brains causative agent cause caused changes in clinical disease clinically clone consolidation coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 vaccine development Cytokines develop effective facilitate feature Genetic hACE2 human ACE2 immunopathology in viral in vivo Infection infectious clones K18-hACE2 K18-hACE2 mice Laboratory lung Lung infection Lung pathology Lungs mCherry mice morbidity Mortality pandemic Pathogenesis Patient Pneumonia Postinfection Produce produced pulmonary Research respiratory respiratory viruses RG-rescued SARS-CoV-2 virus RG-rescued SARS-CoV-2 viruses SARS-CoV-2 SARS-CoV-2 biology SARS-COV-2 infection SARS-CoV-2 isolates SARS-CoV-2 research SARS-CoV-2 toolkit SARS-CoV-2-mCherry severe acute respiratory syndrome Coronavirus severe COVID-19 structures toolkit utility Validity valuable resource variety Viral Viral pneumonia viruses weight loss [DOI] 10.1128/mBio.00819-21 PMC 바로가기 [Article Type] Research Article
Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophagesArticle Published on 2021-04-132022-10-31 Journal: Cell Discovery [Category] COVID-19, [키워드] activated acute respiratory syndrome alveolar macrophage amplification cellular complex coronavirus cytoplasm Degradation endosomal pH endosome facilitate hACE2 Innate immunity Invasion limit Lungs lysosomal lysosome Mechanisms of disease membrane fusion packaged potential therapeutic targets released Replication respond SARS-CoV-2 SARS-COV-2 infection Spread Transgenic mouse Viral RNA virus [DOI] 10.1038/s41421-021-00258-1 PMC 바로가기 [Article Type] Article
Mutations derived from horseshoe bat ACE2 orthologs enhance ACE2-Fc neutralization of SARS-CoV-2편자박쥐 ACE2 orthologs에서 파생된 돌연변이는 SARS-CoV-2의 ACE2-Fc 중화를 향상시킵니다Research Article Published on 2021-04-092022-09-10 Journal: PLoS Pathogens [Category] MERS, SARS, 변종, [키워드] ACE2 ACE2 binding ACE2 ortholog ACE2 orthologs ACE2 proteins ACE2 residue ACE2-Fc acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 bat bat ACE2 bats binding binding affinity both viruses Cell cellular receptor Combination Consensus coronavirus described domain ENhance expressing FIVE hACE2 heterogeneity horseshoe bat horseshoe bats Human human ACE2 Hypothesis identify impacted inactive infect Infection Infectious virus Interaction intermediate introduced investigated Mutation neutralization neutralize neutralized Neutralizing ortholog orthologs pangolin pangolins potency Protein pseudovirus pseudoviruses RaTG13 RBD RBDs receptor receptor ACE2 Receptor-binding domain Region regions residue respiratory S-protein SARS-CoV SARS-CoV-1 SARS-CoV-1 and -2 SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 pseudoviruses SARS-CoV-2 RBD SARS-like coronavirus SARS-like viruses selection pressure severe acute respiratory syndrome Coronavirus soluble ACE2 species susceptibility the binding affinity the RBD the SARS-CoV-2 These data Variability variant Viral viral spike virus wild-type [DOI] 10.1371/journal.ppat.1009501 PMC 바로가기 [Article Type] Research Article
BNT162b2 Vaccine Encoding the SARS-CoV-2 P2 S Protects Transgenic hACE2 Mice against COVID-19Brief Report Published on 2021-04-012022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] Anti-viral approved BNT162b2 challenge study correlated COVID-19 Efficacy elicited encoding hACE2 immunization immunogenicity lung lesion mice mRNA vaccine neutralizing antibody of BNT162b2 Prevent protective effect SARS-CoV-2 the SARS-CoV-2 transgenic mice viral challenge viral clearance [DOI] 10.3390/vaccines9040324 PMC 바로가기 [Article Type] Brief Report